Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives.

Platelet concentrates for topical and infiltrative use - commonly termed Platetet-Rich Plasma (PRP) or Platelet-Rich Fibrin (PRF) - are used or tested as surgical adjuvants or regenerative medicine preparations in most medical fields, particularly in sports medicine and orthopaedic surgery. Even if these products offer interesting therapeutic perspectives, their clinical relevance is largely debated, as the literature on the topic is often confused and contradictory. The long history of these products was always associated with confusions, mostly related to the lack of consensual terminology, characterization and classification of the many products that were tested in the last 40 years. The current consensus is based on a simple classification system dividing the many products in 4 main families, based on their fibrin architecture and cell content: Pure Platelet-Rich Plasma (P-PRP), such as the PRGF-Endoret technique; Leukocyte- and Platelet-Rich Plasma (LPRP), such as Biomet GPS system; Pure Platelet-Rich Fibrin (P-PRF), such as Fibrinet; Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Intra-Spin L-PRF. The 4 main families of products present different biological signatures and mechanisms, and obvious differences for clinical applications. This classification serves as a basis for further investigations of the effects of these products. Perspectives of evolutions of this classification and terminology are also discussed, particularly concerning the impact of the cell content, preservation and activation on these products in sports medicine and orthopaedics.

Echinococcus metacestode: in search of viability markers

Epidemiological studies have demonstrated that most humans infected with Echinococcus spp. exhibit resistance to disease. When infection leads to disease, the parasite is partially controlled by host immunity: in case of immunocompetence, the normal alveolar echinococcosis (AE) or cystic echinococcosis (CE) situation, the metacestode grows slowly, and first clinical signs appear years after infection; in case of impaired immunity (AIDS; other immunodeficiencies), uncontrolled proliferation of the metacestode leads to rapidly progressing disease. Assessing Echinococcus multilocularis viability in vivo following therapeutic interventions in AE patients may be of tremendous benefit when compared with the invasive procedures used to perform biopsies. Current options are F18-fluorodeoxyglucose-positron emission tomography (FDG-PET), which visualizes periparasitic inflammation due to the metabolic activity of the metacestode, and measurement of antibodies against recEm18, a viability-associated protein, that rapidly regresses upon metacestode inactivation. For Echinococcus granulosus, similar prognosis-associated follow-up parameters are still lacking but a few candidates may be listed. Other possible markers include functional and diffusion-weighted Magnetic Resonance Imaging (MRI), and measurement of products from the parasite (circulating antigens or DNA), and from the host (inflammation markers, cytokines, or chemokines). Even though some of them have been promising in pilot studies, none has been properly validated in an appropriate number of patients until now to be recommended for further use in clinical settings. There is therefore still a need to develop reliable tools for improved viability assessment to provide the sufficient information needed to reliably withdraw anti-parasite benzimidazole chemotherapy, and a basis for the development of new alternative therapeutic tools.

The Evolution of Routines under flexible Information Technology

Many technological developments of the past two decades come with the promise of greater IT flexi-bility, i.e. greater capacity to adapt IT. These technologies are increasingly used to improve organiza-tional routines that are not affected by large, hard-to-change IT such as ERP. Yet, most findings on the interaction of routines and IT stem from contexts where IT is hard to change. Our research ex-plores how routines and IT co-evolve when IT is flexible. We review the literatures on routines to sug-gest that IT may act as a boundary object that mediates the learning process unfolding between the ostensive and the performative aspect of the routine. Although prior work has concluded from such conceptualizations that IT stabilizes routines, we qualify that flexible IT can also stimulate change because it enables learning in short feedback cycles. We suggest that, however, such change might not always materialize because it is contingent on governance choices and technical knowledge. We de-scribe the case-study method to explore how routines and flexible IT co-evolve and how governance and technical knowledge influence this process. We expect to contribute towards stronger theory of routines and to develop recommendations for the effective implementation of flexible IT in loosely coupled routines.

Fortaleciéndo la soberanía alimentaria mediante la revalorización de saberes ecológicos locales: experiencia en los Andes bolivianos

Ethnobiology research contributes significantly to initiatives that aim to enhance food sovereignty among indigenous and/or traditional people. In Bolivia, one of the Latin-American countries that shows the highest poverty and undernourishment levels, the purpose of this research-action project was to enhance food sovereignty through the revitalization of the local ecological knowledge and to promote local technological innovation processes in the Andean community of Tallija-Confital. During a first step the endogenous knowledge and strategies related to food security and sovereignty were investigated, based on the principles and tools of the Revitalizing Participatory Research (RPR). In a second step local technical innovation processes were supported through a “knowledge dialogue” between exogenous and endogenous knowledge systems, focusing on the processing of the cañahua (Chenopodium pallidicaule Aellen) gluten. The research results demonstrate that Andean people have developed complex endogenous knowledge and strategies to adapt to socio-environmental changes that show a great potential to contribute to the enhancement of food sovereignty. Nevertheless, in the current globalized context that translates into new challenges for local communities, beyond the revitalization of local ecological knowledge, a dialogue between different knowledge systems can lead to important local technological innovation for the improvement of their well-being. Key words: food sovereignty, knowledge dialogue, endogenous development, technological innovation

SUBCELLULAR LOCALIZATION, PURIFICATION AND PROPERTIES OF A CDP-DIACYLGLYCEROL - DEPENDENT PHOSPHATIDYLSERINE SYNTHASE IN BACILLUS LICHENIFORMIS

A CDP-diacylglycerol dependent phosphatidylserine synthase was detected in three species of gram-positive bacilli, viz. Bacillus licheniformis, Bacillus subtilis and Bacillus megaterium; the enzyme in B. licheniformis was studied in detail. The subcellular distribution experiments in cell-free extracts of B. licheniformis using differential centrifugation, sucrose gradient centrifugation and detergent solubilization showed the phosphatidylserine synthase to be tightly associated with the membrane. The enzyme was shown to have an absolute requirement for divalent metal ion for activity with a strong preference for manganese. The enzyme activity was completely dependent upon the addition of CDP-diacylglycerol to the assay system; the role of the liponucleotide was rigorously shown to be that of phosphatidyl donor and not just a detergent-like stimulator. This enzyme was then solubilized from B. licheniformis membranes and purified to near homogeneity. The purification procedure consisted of CDP-diacylglycerol-Sepharose affinity chromatography followed by substrate elution from blue-dextran Sepharose. The purified preparation showed a single band with an apparent minimum molecular weight of 53,000 when subjected to SDS polyacrylamide gel electrophoresis. The preparation was free of any phosphatidylglycerophosphate synthase, CDP-diacylglycerol hydrolase and phosphatidylserine hydrolase activities. The utilization of substrates and formation of products occurred with the expected stoichiometry. Radioisotopic exchange patterns between related substrate and product pairs suggest a sequential BiBi reaction as opposed to the ping-pong mechanism exhibited by the well studied phosphatidylserine synthase of Escherichia coli. Proteolytic digestion of the enzyme yielded a smaller active form of the enzyme (41,000 daltons) which appears to be less prone to aggregation.^ This has been the first detailed study in a well-defined bacillus species of the enzyme catalyzing the CDP-diacylglycerol-dependent formation of phosphatidylserine; this reaction is the first committed step in the biosynthetic pathway to the major membrane component, phosphatidylethanolamine. Further study of this enzyme may lead to understanding of new mechanisms of phosphatidyl transfer and novel modes of control of phospholipid biosynthetic enzymes. ^

The status of genetically modified foods internationally and in the United States

Biotechnology refers to the broad set of techniques that allow genetic manipulation of organisms. The techniques of biotechnology have broad implications for many industries, however it promises the greatest innovations in the production of products regulated by the Food and Drug Administration (FDA). Like many other powerful new technologies, biotechnology may carry risks as well as benefits. Several of its applications have engendered fervent emotional reactions and raised serious ethical concerns, especially internationally. ^ First, in my paper I discuss the historical and technical background of biotechnology. Second, I examine the development of biotechnology in Europe, the citizens' response to genetically modified (“GM”) foods and the governments' response. Third, I examine the regulation of bioengineered products and foods in the United States. ^ In conclusion, there are various problems with the current status of regulation of GM foods in the United States. These are four basic flaws: (1) the Coordinated Framework allows for too much jurisdictional overlap of biotechnological foods, (2) GM foods are considered GRAS and consequently, are placed on the market without pre-market approval, (3) federal mandatory labeling of GM foods cannot occur until the question of whether or not nondisclosure of a genetic engineering production processes is misleading or material information and (4) an independent state-labeling scheme of GM foods will most likely impede interstate commerce. ^

La tempestad y el progreso : una aproximación a la sociedad global de la información

En las últimas décadas, la sociedad de la información se ha convertido en una cuestión de debate. Es un paradigma emergente sobre los nuevos modelos de ciencia, de producción de conocimiento e innovación tecnológica. Complejidad y cambio son dos características definidoras de este fenómeno sistémico. El autor propone una aproximación a los desafíos y problemas más importantes en la era de la información. Según los pensadores analizados, esta área de investigación comprende el conjunto de nuevas tecnologías en un proceso multidimensional relacionado con el desarrollo del capitalismo global, y particularmente con la expansión de las empresas multinacionales. La sociedad de la información representa un nuevo orden mundial, sustentado en el mito ideológico y hegemónico del progreso. El artículo tiene dos objetivos: a) identificar algunas de las principales categorías y conceptos utilizados en la discusión; b) presentar un esquema desarrollado para el examen de sus impactos y consecuencias.

Producir para el agro en un entorno turbulento. El caso de una fábrica de cosechadoras en la Argentina

La producción de maquinaria agrícola en la Argentina se inicia muy tempranamente, estimulada por las amplias posibilidades que brindaba la producción de cereales. Un rasgo destacable del sector se refiere a la importante tradición en innovación tecnológica, sin duda alentada por la naturaleza específica de la producción agrícola en nuestro país y por los altos costos de las maquinas importadas. Esta tradición en innovación y el entramado territorial resultarán claves para entender el desarrollo de una firma fabricante de cosechadoras en el sur de Santa Fe a partir de la década del cincuenta. Pueden identificarse cuatro grandes etapas en la trayectoria de Roque Vassalli SA; cada una de estas etapas presentadas en el trabajo de manera cronológica enfatiza algún aspecto de la dinámica empresarial que explica el desempeño productivo y la evolución económico-financiera de la firma en ese período. De este modo, en una primera etapa se destacan la disposición innovadora del empresario fundador, su ensamblaje con las políticas públicas de promoción del sector y la fuerte apuesta a la conformación de una red de distribuidores como factores claves para la consolidación de la empresa en el mercado interno. En cambio, en una segunda etapa se enfatiza en los problemas que presentó el mercado de maquinarias agrícolas en la segunda mitad de los años sesenta que terminaron por afectar su situación productiva y financiera. En el tercer apartado el trabajo enfoca las variables que permiten explicar la recuperación de la empresa, principalmente en la importante dinámica del mercado interno pero también en las estrategias de comercialización y la captura de amplios mercados externos. Finalmente, el análisis en la cuarta etapa destaca los límites estrechos que la coyuntura macroeconómica impuso a la gestión empresarial y que terminó con la venta de la empresa.

Producir para el agro en un entorno turbulento. El caso de una fábrica de cosechadoras en la Argentina

La producción de maquinaria agrícola en la Argentina se inicia muy tempranamente, estimulada por las amplias posibilidades que brindaba la producción de cereales. Un rasgo destacable del sector se refiere a la importante tradición en innovación tecnológica, sin duda alentada por la naturaleza específica de la producción agrícola en nuestro país y por los altos costos de las maquinas importadas. Esta tradición en innovación y el entramado territorial resultarán claves para entender el desarrollo de una firma fabricante de cosechadoras en el sur de Santa Fe a partir de la década del cincuenta. Pueden identificarse cuatro grandes etapas en la trayectoria de Roque Vassalli SA; cada una de estas etapas presentadas en el trabajo de manera cronológica enfatiza algún aspecto de la dinámica empresarial que explica el desempeño productivo y la evolución económico-financiera de la firma en ese período. De este modo, en una primera etapa se destacan la disposición innovadora del empresario fundador, su ensamblaje con las políticas públicas de promoción del sector y la fuerte apuesta a la conformación de una red de distribuidores como factores claves para la consolidación de la empresa en el mercado interno. En cambio, en una segunda etapa se enfatiza en los problemas que presentó el mercado de maquinarias agrícolas en la segunda mitad de los años sesenta que terminaron por afectar su situación productiva y financiera. En el tercer apartado el trabajo enfoca las variables que permiten explicar la recuperación de la empresa, principalmente en la importante dinámica del mercado interno pero también en las estrategias de comercialización y la captura de amplios mercados externos. Finalmente, el análisis en la cuarta etapa destaca los límites estrechos que la coyuntura macroeconómica impuso a la gestión empresarial y que terminó con la venta de la empresa.

Producir para el agro en un entorno turbulento. El caso de una fábrica de cosechadoras en la Argentina

La producción de maquinaria agrícola en la Argentina se inicia muy tempranamente, estimulada por las amplias posibilidades que brindaba la producción de cereales. Un rasgo destacable del sector se refiere a la importante tradición en innovación tecnológica, sin duda alentada por la naturaleza específica de la producción agrícola en nuestro país y por los altos costos de las maquinas importadas. Esta tradición en innovación y el entramado territorial resultarán claves para entender el desarrollo de una firma fabricante de cosechadoras en el sur de Santa Fe a partir de la década del cincuenta. Pueden identificarse cuatro grandes etapas en la trayectoria de Roque Vassalli SA; cada una de estas etapas presentadas en el trabajo de manera cronológica enfatiza algún aspecto de la dinámica empresarial que explica el desempeño productivo y la evolución económico-financiera de la firma en ese período. De este modo, en una primera etapa se destacan la disposición innovadora del empresario fundador, su ensamblaje con las políticas públicas de promoción del sector y la fuerte apuesta a la conformación de una red de distribuidores como factores claves para la consolidación de la empresa en el mercado interno. En cambio, en una segunda etapa se enfatiza en los problemas que presentó el mercado de maquinarias agrícolas en la segunda mitad de los años sesenta que terminaron por afectar su situación productiva y financiera. En el tercer apartado el trabajo enfoca las variables que permiten explicar la recuperación de la empresa, principalmente en la importante dinámica del mercado interno pero también en las estrategias de comercialización y la captura de amplios mercados externos. Finalmente, el análisis en la cuarta etapa destaca los límites estrechos que la coyuntura macroeconómica impuso a la gestión empresarial y que terminó con la venta de la empresa.

(Table 4) Concentrations of radionuclides in waters of the Barents and Kara Seas in August 2003

A methodology of experimental simulation of state of spent nuclear fuel that occurs on the sea floor due to some catastrophes or dumping is developed. Data on long-term (more than 2000 days) experiments on estimation of 85Kr and 137Cs release rate from spent nuclear fuel (fragments of irradiated UO2 pellets) were firstly obtained; these estimates prove correctness of a hypothesis offered by us in early 1990s concerning to earlier 85Kr release (by one order of magnitude higher than that of 137Cs) as compared to other fission fragments in case of loss of integrity of fuel containment as a result of corrosion on the sea floor. A method and technique of onboard 85Kr and 137Cs sampling and extraction (as well as sampling of tritium, product of triple 235U fission) and their radiometric analysis at coastal laboratories are developed. Priority data on 85Kr background in bottom layers of the Barents and Kara Seas and 137Cs and 3H in these seas (state of 2003) are presented. Models necessary for estimation of dilution of fission products of spent nuclear fuel and their transport on the floor in accident and dumping regions are developed. An experimental method for examination of state of spent nuclear fuel on the sea floor (one expedition each 2-3 years) by 85Kr release into environment (a leak tracer) is proposed; this release is an indicator of destruction of fuel containment and release of products of spent nuclear fuel in case of 235UO2 corrosion in sea water.

Results of biogeochemical studies in the Russian Arctic seas: isotope and radioisotope data

Comprehensive biogeochemical studies including determination of isotopic composition of organic carbon in both suspended matter and surface layer (0-1 cm) bottom sediments (more than 260 determinations of d13C-Corg) were carried out for five Arctic shelf seas: White, Barents, Kara, East Siberian, and Chukchi Seas. The aim of this study is to elucidate causes that change isotopic composition of particulate organic carbon at the water-sediment boundary. It is shown that isotopic composition of organic carbon in sediments from seas with high river run-off (White, Kara, and East Siberian Seas) does not inherit isotopic composition of organic carbon in particles precipitating from the water column, but is enriched in 13C. Seas with low river run-off (Barents and Chukchi Seas) show insignificant difference between d13C-Corg values in both suspended load and sediments because of low content of isotopically light allochthonous organic matter in suspended matter. Biogeochemical studies with radioisotope tracers (14CO2, 35S, and 14CH4) revealed existence of specific microbial filter formed from heterotrophic and autotrophic organisms at the water-sediment boundary. This filter prevents mass influx of products of organic matter decomposition into the water column, as well as reduces influx of OM contained in suspended matter from water into sediments.

How did the crisis affect small and medium-sized enterprises? -- from a field study of the metal-working industry in Java

This paper focuses on the impact of Indonesia's economic crisis on small and medium-sized enterprises (SMEs). It shows how the performance of SMEs during the crisis varied widely even in the same industrial subsector, and found that the factors most affecting performance have been market orientation and the linkages that the SMEs have formed with the buyers of their products. Well-performing SMEs were found to have utilized putting-out linkages with wholesalers which enabled them to switch to products having better markets. On the other hand, the SMEs which had subcontracting linkages with assemblers or contracting linkages with user-factories (with the exception of SMEs having export-oriented linkages) suffered badly in the crisis because of specificity of products with little room for switching. The paper also found that exposure to debt due to borrowing for investment has been another factor affecting performance, but that enterprise size has had no linear correlation with performance.

Technology network for machine tools in Vietnam

When Vietnam joined the WTO, it accepted foreign direct investment and started to grow. Technically, it was then greatly influenced by the enterprises that entered the country through direct investment. This report shows that the technology network for machine tools is formed via direct investment and subcontracting.

Selection and utilization of the early harvest list : evidence from the Free Trade Agreement between China and Taiwan

In this paper, we conducted an empirical investigation into the determinants of FTA utilization in exports from Taiwan to China. To do this, we first estimated the selection equation to see what kinds of products are included in the early harvest list. As a result, we found that Taiwan includes products with a medium magnitude of benefits from tariff removal in the early harvest list. Taiwan also includes products for which ASEAN countries have better access to the China market. We then estimated the equation for the determinants of FTA utilization by introducing an inverse of the Mills ratio estimated in the selection equation. The findings are that, as usual, the FTA rates are more likely to be utilized for products with a larger tariff margin. In addition, some rules of origin are found to be relatively restrictive in terms of discouraging trade.