In vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to Staphylococcus aureus and yeast species

We have studied the effect of inactivated microbial stimuli (Candida albicans, Candida glabrata, Saccharomyces boulardii, and Staphylococcus aureus) on the in vitro differentiation of lineage negative (Lin−) hematopoietic progenitor mouse cells. Purified Lin− progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. All the stimuli assayed caused differentiation toward the myeloid lineage. S. boulardii and particularly C. glabrata were the stimuli that induced in a minor extent differentiation of Lin− cells, as the major population of differentiated cells corresponded to monocytes, whereas C. albicans and S. aureus induced differentiation beyond monocytes: to monocyte-derived dendritic cells and macrophages, respectively. Interestingly, signaling through TLR2 by its pure ligand Pam3CSK4 directed differentiation of Lin− cells almost exclusively to macrophages. These data support the notion that hematopoiesis can be modulated in response to microbial stimuli in a pathogen-dependent manner, being determined by the pathogen-associated molecular patterns and the pattern-recognition receptors involved, in order to generate the populations of mature cells required to deal with the pathogen.

Diferencias en las atribuciones académicas en lengua en base al efecto positivo

La finalidad de esta investigación residió en conocer si los individuos pertenecientes a población infantil española con altas puntuaciones en Afecto Positivo (AP) poseían diferencias estadísticamente significativas con los individuos con bajas puntuaciones en AP en las seis dimensiones que componen las atribuciones académicas en el área de Lengua (Éxito: capacidad, esfuerzo y causas externas; Fracaso: capacidad, esfuerzo y causas externas). La muestra estaba compuesta por 862 estudiantes, entre 8 y 11 años. Se utilizó la subescala de lengua de la Sydney Attribution Scale (SAS) y la subescala de AP del Positive And Negative Affect Schedule for Children (PANAS-C) versión reducida. La prueba t de Student reflejó que los participantes con altos niveles de AP obtuvieron medias significativamente más altas en la atribución al éxito y al fracaso en Lengua a causas externas. Por otro lado, los sujetos con bajas puntuaciones en AP obtuvieron medias significativamente más altas en la atribución del fracaso en Lengua a la capacidad y al esfuerzo. Los resultados obtenidos complementan la literatura previa existente y dejan la posibilidad a seguir ampliando el campo del conocimiento del afecto y de las atribuciones académicas.

The nasal microbiota in infants with cystic fibrosis in the first year of life: a prospective cohort study.

BACKGROUND Respiratory tract infections and subsequent airway inflammation occur early in the life of infants with cystic fibrosis. However, detailed information about the microbial composition of the respiratory tract in infants with this disorder is scarce. We aimed to undertake longitudinal in-depth characterisation of the upper respiratory tract microbiota in infants with cystic fibrosis during the first year of life. METHODS We did this prospective cohort study at seven cystic fibrosis centres in Switzerland. Between Feb 1, 2011, and May 31, 2014, we enrolled 30 infants with a diagnosis of cystic fibrosis. Microbiota characterisation was done with 16S rRNA gene pyrosequencing and oligotyping of nasal swabs collected every 2 weeks from the infants with cystic fibrosis. We compared these data with data for an age-matched cohort of 47 healthy infants. We additionally investigated the effect of antibiotic treatment on the microbiota of infants with cystic fibrosis. Statistical methods included regression analyses with a multivariable multilevel linear model with random effects to correct for clustering on the individual level. FINDINGS We analysed 461 nasal swabs taken from the infants with cystic fibrosis; the cohort of healthy infants comprised 872 samples. The microbiota of infants with cystic fibrosis differed compositionally from that of healthy infants (p=0·001). This difference was also found in exclusively antibiotic-naive samples (p=0·001). The disordering was mainly, but not solely, due to an overall increase in the mean relative abundance of Staphylococcaceae in infants with cystic fibrosis compared with healthy infants (multivariable linear regression model stratified by age and adjusted for season; second month: coefficient 16·2 [95% CI 0·6-31·9]; p=0·04; third month: 17·9 [3·3-32·5]; p=0·02; fourth month: 21·1 [7·8-34·3]; p=0·002). Oligotyping analysis enabled differentiation between Staphylococcus aureus and coagulase-negative Staphylococci. Whereas the analysis showed a decrease in S aureus at and after antibiotic treatment, coagulase-negative Staphylococci increased. INTERPRETATION Our study describes compositional differences in the microbiota of infants with cystic fibrosis compared with healthy controls, and disordering of the microbiota on antibiotic administration. Besides S aureus, coagulase-negative Staphylococci also contributed to the disordering identified in these infants. These findings are clinically important in view of the crucial role that bacterial pathogens have in the disease progression of cystic fibrosis in early life. Our findings could be used to inform future studies of the effect of antibiotic treatment on the microbiota in infants with cystic fibrosis, and could assist in the prevention of early disease progression in infants with this disorder. FUNDING Swiss National Science Foundation, Fondation Botnar, the Swiss Society for Cystic Fibrosis, and the Swiss Lung Association Bern.

The influence of the rearing period on intramammary infections in Swiss dairy heifers: A cross-sectional study

Healthy replacement heifers are one of the foundations of a healthy dairy herd. Farm management andrearing systems in Switzerland provide a wide variety of factors that could potentially be associated withintramammary infections (IMI) in early lactating dairy heifers. In this study, IMI with minor mastitispathogens such as coagulase-negative staphylococci (CNS), contagious pathogens, and environmentalmajor pathogens were identified. Fifty-four dairy farms were enrolled in the study. A questionnaire wasused to collect herd level data on housing, management and welfare of young stock during farm isitsand interviews with the farmers. Cow-level data such as breed, age at first calving, udder condition andswelling, and calving ease were also recorded. Data was also collected about young stock that spent aperiod of at least 3 months on an external rearing farm or on a seasonal alpine farm. At the quarterlevel, teat conditions such as teat lesions, teat dysfunction, presence of a papilloma and teat lengthwere recorded. Within 24 h after parturition, samples of colostral milk from 1564 quarters (391 heifers)were collected aseptically for bacterial culture. Positive bacteriological culture results were found in 49%of quarter samples. Potential risk factors for IMI were identified at the quarter, animal and herd levelusing multivariable and multilevel logistic regression analysis. At the herd level tie-stalls, and at cow-level the breed category “Brown cattle” were risk factors for IMI caused by contagious major pathogenssuch as Staphylococcus aureus (S. aureus). At the quarter-level, teat swelling and teat lesions were highlyassociated with IMI caused by environmental major pathogens. At the herd level heifer rearing at externalfarms was associated with less IMI caused by major environmental pathogens. Keeping pregnant heifersin a separate group was negatively associated with IMI caused by CNS. The odds of IMI with coagulase-negative staphylococci increased if weaning age was less than 4 months and if concentrates were fed tocalves younger than 2 weeks. This study identified herd, cow- and quarter-level risk factors that may beimportant for IMI prevention in the future.

Concepto positivo del delito,

Mode of access: Internet.

L'economia politica studiata col metodo positivo : conferenze.

Mode of access: Internet.

Jurisprudentia publica universalis : ex jure tum naturali, tum divino positivo : nec non jure gentium nova et scientifica methodo derivata et juris publici facta /

Mode of access: Internet.

Jurisprudentia publica universalis : ex jure tum naturali, tum divino positivo : nec non jure gentium nova et scientifica methodo derivata et juris publici facta /

Mode of access: Internet.

De perfectionibus divinis opus theologicum positivo-scholasticum ...

Mode of access: Internet.

De perfectionibus divinis opus theologicum positivo-scholasticum ...

Mode of access: Internet.

[Que la caridad evangélica ha recibido un desarrollo positivo en las Órdenes religiosas, especialmente en la Real y militar Órden de Nuestra Señora de la Merced] : discurso leído en la Universidad Central /

Tesis doctoral Universidad Central (Madrid), 1860.

Elementos de derecho internacional público : precedidos de una introduccion a su estudio bajo los dos aspectos de su desarrollo histórico ó positivo y de su teoría /

Son 1 t. en 2 v.

Derecho positivo y consuetudinario en Latinoamérica : La jurisdicción sobre la subalternidad en el Bicentenario de la Independencia

El trabajo se aproximará a la problemática del pluralismo jurídico abordando la misma desde una perspectiva sociológica, con el fin de plantear una mirada transversal de las normativas vigentes en la República de Colombia, tomando a este como un caso emblemático en Latinoamérica, así como también de las bases filosóficas que dan un fundamento a esta práctica jurídica. La construcción de una nueva forma del derecho positivo en la región, a partir de la integración de las jurisdicciones especiales indígenas a la Constitución Política de los diversos países y su coordinación con los sistemas judiciales nacionales, es un objeto digno de atención en tanto, de su correcta aplicación y permanente observación, depende la integración de diversos grupos subalternos a la estructura de la sociedad mayoritaria en el subcontinente. El análisis de la experiencia Colombiana y la jurisprudencia vigente en este país, puede guiar y pautar la puesta en práctica de esta forma de pluralismo en el resto de la región, la cual es impulsada, en primer lugar, por los propios representantes del derecho positivo que intenta modificarse a partir de la misma

Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin genes: Worldwide emergence

Infections caused by community-acquired (CA)-methicillin-resistant Staphylococcus aureus (MRSA) have been reported worldwide. We assessed whether any common genetic markers existed among 117 CA-MRSA isolates from the United States, France, Switzerland, Australia, New Zealand, and Western Samoa by performing polymerase chain reaction for 24 virulence factors and the methicillin-resistance determinant. The genetic background of the strain was analyzed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The CA-MRSA strains shared a type IV SCCmec cassette and the Panton-Valentine leukocidin locus, whereas the distribution of the other toxin genes was quite specific to the strains from each continent. PFGE and MLST analysis indicated distinct genetic backgrounds associated with each geographic origin, although predominantly restricted to the agr3 background. Within each continent, the genetic background of CA-MRSA strains did not correspond to that of the hospital-acquired MRSA.

Antimicrobial resistance in Staphylococcus aureus in Australian teaching hospitals, 1989-1999

An annual survey of antimicrobial resistance in clinical isolates of Staphylococcus aureus was conducted in 21 Australian teaching hospital microbiology laboratories in eight major cities from 1989 to 1999. A total of 19,000 isolates were tested for susceptibility to 18 antimicrobials, with 3795 being methicillin-resistant (MRSA). Resistance to ciprofloxacin in MRSA increased from 4.9% to 75.9%. The proportion of MRSA resistant to erythromycin decreased significantly (99.0%-88.9%), as did that to trimethoprim (98.4%-82.4%) and to tetracycline (96.5%-80.1%). The proportion of MRSA isolated increased in Sydney, Melbourne, Canberra, Adelaide, Perth, and Darwin, but not in Brisbane. The proportion in Hobart peaked in 1994. MRSA in Perth were predominantly non-multiresistant (nmMRSA) throughout the survey (i.e., resistant to less than three of eight indicator antibiotics) due mainly to local strains that originated in the community. The proportion of nmMRSA increased to modest levels in the other cities. In eastern cities, this was due to the appearance of strains closely related to nmMRSA seen in other countries of the southwestern Pacific.