The importance of periodic droughts for maintaining diversity in the freshwater environment

Whereas some species may rely on periodic drought conditions for part of their life histories, or have life strategies suited to exploiting the habitat or changed environmental conditions that are created by drought, for other organisms it is a time of stress. Periodic drought conditions therefore generate a series of waves of colonization and extinctions. Studies on lowland wet grassland, in winterbournes and in the toiche zone of both ponds and rivers, also demonstrate that different organisms are competitively favoured with changing hydrological conditions, and that this process prevents any one species from overwhelming its competitors. Competitive impacts may be inter- and intraspecific. It is therefore apparent that the death of organisms such as adult fish during severe drought conditions, though traumatic for human onlookers and commercial interests, may be merely a regular occurrence to which the ecosystem is adapted. The variability of climatic conditions thereby provides a direct influence on the maintenance of biological diversity, and it is this very biodiversity that provides the ecosystem with the resilience to respond to environmental changes in both the short and the longer term.

Molecular genetic studies on voltage-gated ion channels

Several different methods have been employed in the study of voltage-gated ion channels. Electrophysiological studies on excitable cells in vertebrates and molluscs have shown that many different voltage-gated potassium (K⁺) channels and sodium channels may coexist in the same organism. Parallel genetic studies in Drosophila have identified mutations in several genes that alter the properties of specific subsets of physiologically identified ion channels. Chapter 2 describes molecular studies that identify two Drosophila homologs of vertebrate sodium-channel genes. Mutations in one of these Drosophila sodium-channel genes are shown to be responsible for the temperature-dependent paralysis of a behavioural mutant para^ts. Evolutionary arguments, based on the partial sequences of the two Drosophila genes, suggest that subfamilies of voltage-gated sodium channels in vertebrates remain to be identified.

In Drosophila, diverse voltage-gated K⁺ channels arise from alternatively spliced mRNAs generated at the Shaker locus. Chapter 3 and the Appendices describe the isolation and characterization of several human K⁺-channel genes, similar in sequence to Shaker. Each of these human genes has a highly conserved homolog in rodents; thus, this K⁺-channel gene family probably diversified prior to the mammalian radiation. Functional K⁺ channels encoded by these genes have been expressed in Xenopus oocytes and their properties have been analyzed by electrophysiological methods. These studies demonstrate that both transient and noninactivating voltage-gated K⁺ channels may be encoded by mammalian genes closely related to Shaker. In addition, results presented in Appendix 3 clearly demonstrate that independent gene products from two K⁺-channel genes may efficiently co-assemble into heterooligomeric K⁺ channels with properties distinct from either homomultimeric channel. This finding suggests yet another molecular mechanism for the generation of K⁺-channel diversity.

Environmental variables and fisheries diversity of the Naaf River Estuary, Bangladesh

The Naaf River estuary is one of the large estuaries in the Bangladesh coastal region not to have been affected by extensive human disturbance. This research provides information about the fisheries diversity status by Estuarine Set Bag Net (ESBN) sampling relation to physicochemical variables in both spatio-temporal scales. About 25 km of the lower estuary was divided into six zones for sample collection by considering the accessibility and availability of the ESBN operation, fish landing centers and location of the fishing villages. In total 48 samples have been analyzed which were taken throughout March to October 2006. To quantify the species diversity, all fisheries data were analyzed by using EstimateS and EcoSim software which accounts the different diversity indices viz., species richness, Shannon–Wiener diversity Index, Dominance and Evenness index. The research results demonstrate that the Naaf River estuary is a habitat of 161 (species richness, Sobs=161, Choa 1=162±2.34, ACE=161.73) different species which belong to 98 fin fishes, 23 shrimps and prawns,13 crabs, 11 molluscs, 3 echinoderms, 4 other crustaceans;while 9 remain unidentified. Results on the aquatic environment,mainly salinity and turbidity were found to have a major influence on their occurrence and distribution. All the findings indicated that the Naaf River estuary is a highly productive system and provides a favourable environment for large variety of estuarine species assemblages.

The diversity and ecological role of protozoa in fresh waters

Protozoa feed on and regulate the abundance of most types of aquatic microorganisms, and they are an integral part of all aquatic microbial food webs. Being so small, aerobic protozoa thrive at low oxygen tensions, where they feed (largely unaffected by metazoan grazing) on the abundance of other microorganisms. In anaerobic environments, they are the only phagotrophic organisms, and they live in unique symbiotic consortia with methanogens, sulphate reducers and non-sulphur purple bacteria. The number of extant species of protozoa may be quite modest (the global number of ciliate species is estimated at 3000), and most of them probably have cosmopolitan distributions. This will undoubtedly make it easier to carry out further tasks, e.g. understanding the role of protozoan species diversity in the natural environment.

A multimodalidade no livro didático de inglês como língua estrangeira: padrões de representação narrativa e de interação

O presente trabalho visa a contribuir com o avanço das pesquisas na área de multimodalidade, mais especificamente na área aplicada ao contexto de ensino de língua estrangeira. Analisa-se uma amostra de textos multimodais em um livro didático produzido e utilizado no Brasil como ferramenta para o ensino de inglês como língua estrangeira para alunos adultos iniciantes em um curso livre. Tendo em vista a preocupação, apontada no próprio material didático, em atender às necessidades e expectativas desses alunos, objetiva-se, através desta investigação: verificar como se dá a interação entre o verbal e o visual no livro didático selecionado; verificar como essa interação contribui para atingir os objetivos pedagógicos propostos pelo material; e, por fim, contribuir, de alguma maneira, para o letramento multimodal de alunos em língua estrangeira. Tais objetivos determinam a natureza híbrida desta pesquisa que, além da sua dimensão analítico-descritiva, apresenta também uma dimensão pedagógica, que visa a apresentar propostas de trabalho multimodal com algumas das atividades selecionadas para análise. A seleção dos textos multimodais para a composição do corpus desta pesquisa foi baseada na observação da recorrência de imagens com determinados personagens ao longo do livro. Tal recorrência provocou questionamentos que só poderiam ser respondidos a partir da análise desses personagens representados em situações de (inter)ação, o que deu lugar à seleção das representações narrativas que os incluíssem. Os personagens em questão são desenhos criados para os fins pedagógicos do material e são representados em situações sociais muito limitadas: a maior parte dessas representações parece formar uma sequência narrativa cuja interação acontece em uma festa; entre as outras representações, que não representam a referida festa como contexto, incluem-se interações no escritório, no restaurante, no parque e ao telefone. Uma análise da representação visual desses atores sociais revelou que, apesar da inclusão de uma negra entre os personagens, e a consequente suposta visão multicultural transmitida com essa inclusão, os participantes representam um grupo homogêneo, pertencentes ao mesmo segmento social, que só interagem entre eles mesmos em situações sociais limitadas, não sendo, portanto, representativos da diversidade étnica, social e cultural do Brasil, ou dos países em que o inglês é falado. Após a análise da representação dos atores sociais, analisam-se, com vistas a atingir os objetivos deste trabalho, os padrões de representação e de interação nos textos multimodais selecionados, segundo categorias do quadro da multimodalidade de van Leeuwen (1996). Verifica-se, a partir de tais análises, que o verbal e o visual nem sempre apresentam uma relação direta, e que, quando apresentam, tal relação não é explorada pelo material, tornando o visual um elemento meramente decorativo que, na maioria das vezes, em nada contribui para o desenvolvimento das unidades. Por essa razão, e por se tratar de uma pesquisa centrada no contexto pedagógico, propõem-se, ao final das análises, atividades de exploração de alguns dos textos multimodais analisados, visando à formação multimodal do aluno em língua estrangeira

Adaptive image sequence coding based on global and local compensability analysis

Our study of a novel technique for adaptive image sequence coding is reported. The number of reference frames and the intervals between them are adjusted to improve the temporal compensability of the input video. The bits are distributed more efficiently on different frame types according to temporal and spatial complexity of the image scene. Experimental results show that this dynamic group-of-picture (GOP) structure coding scheme is not only feasible but also better than the conventional fixed GOP method in terms of perceptual quality and SNR. (C) 1996 Society of Photo-Optical Instrumentation Engineers.

Insight on how fishing bats discern prey and adjust their mechanic and sensorial features during the attack sequence

Several insectivorous bats have included fish in their diet, yet little is known about the processes underlying this trophic shift. We performed three field experiments with wild fishing bats to address how they manage to discern fish from insects and adapt their hunting technique to capture fish. We show that bats react only to targets protruding above the water and discern fish from insects based on prey disappearance patterns. Stationary fish trigger short and shallow dips and a terminal echolocation pattern with an important component of the narrowband and low frequency calls. When the fish disappears during the attack process, bats regulate their attack increasing the number of broadband and high frequency calls in the last phase of the echolocation as well as by lengthening and deepening their dips. These adjustments may allow bats to obtain more valuable sensorial information and to perform dips adjusted to the level of uncertainty on the location of the submerged prey. The observed ultrafast regulation may be essential for enabling fishing to become cost-effective in bats, and demonstrates the ability of bats to rapidly modify and synchronise their sensorial and motor features as a response to last minute stimulus variations.

Viral metagenomic analysis of feces of wild small carnivores

Background: Recent studies have clearly demonstrated the enormous virus diversity that exists among wild animals. This exemplifies the required expansion of our knowledge of the virus diversity present in wildlife, as well as the potential transmission of these viruses to domestic animals or humans. Methods: In the present study we evaluated the viral diversity of fecal samples (n = 42) collected from 10 different species of wild small carnivores inhabiting the northern part of Spain using random PCR in combination with next-generation sequencing. Samples were collected from American mink (Neovison vison), European mink (Mustela lutreola), European polecat (Mustela putorius), European pine marten (Martes martes), stone marten (Martes foina), Eurasian otter (Lutra lutra) and Eurasian badger (Meles meles) of the family of Mustelidae; common genet (Genetta genetta) of the family of Viverridae; red fox (Vulpes vulpes) of the family of Canidae and European wild cat (Felis silvestris) of the family of Felidae. Results: A number of sequences of possible novel viruses or virus variants were detected, including a theilovirus, phleboviruses, an amdovirus, a kobuvirus and picobirnaviruses. Conclusions: Using random PCR in combination with next generation sequencing, sequences of various novel viruses or virus variants were detected in fecal samples collected from Spanish carnivores. Detected novel viruses highlight the viral diversity that is present in fecal material of wild carnivores.

Human LDL Structural Diversity Studied by IR Spectroscopy

Lipoproteins are responsible for cholesterol traffic in humans. Low density lipoprotein (LDL) delivers cholesterol from liver to peripheral tissues. A misleading delivery can lead to the formation of atherosclerotic plaques. LDL has a single protein, apoB-100, that binds to a specific receptor. It is known that the failure associated with a deficient protein-receptor binding leads to plaque formation. ApoB-100 is a large single lipid-associated polypeptide difficulting the study of its structure. IR spectroscopy is a technique suitable to follow the different conformational changes produced in apoB-100 because it is not affected by the size of the protein or the turbidity of the sample. We have analyzed LDL spectra of different individuals and shown that, even if there are not big structural changes, a different pattern in the intensity of the band located around 1617 cm 21 related with strands embedded in the lipid monolayer, can be associated with a different conformational rearrangement that could affect to a protein interacting region with the receptor.