Multi-agent simulation of competitive electricity markets: Autonomous systems cooperation for European market modeling

The electricity market restructuring, and its worldwide evolution into regional and even continental scales, along with the increasing necessity for an adequate integration of renewable energy sources, is resulting in a rising complexity in power systems operation. Several power system simulators have been developed in recent years with the purpose of helping operators, regulators, and involved players to understand and deal with this complex and constantly changing environment. The main contribution of this paper is given by the integration of several electricity market and power system models, respecting to the reality of different countries. This integration is done through the development of an upper ontology which integrates the essential concepts necessary to interpret all the available information. The continuous development of Multi-Agent System for Competitive Electricity Markets platform provides the means for the exemplification of the usefulness of this ontology. A case study using the proposed multi-agent platform is presented, considering a scenario based on real data that simulates the European Electricity Market environment, and comparing its performance using different market mechanisms. The main goal is to demonstrate the advantages that the integration of various market models and simulation platforms have for the study of the electricity markets’ evolution.

Organizational Culture in Cooperatives: an exploratory approach

In recent years, organizational culture has become one of the common themes of interest of scientific and academic research. Each organization has its own unique cultural identity. Based on the recognition that organizational culture is considered important to an organization’s results, and social economy organizations are concerned with improving managerial practices and results, our objective is to study organizational culture in cooperatives: identifying their organizational culture as a specific type of organization of the social economy, recognized as increasingly important economic agents; and in doing so, explore the usage of a widely known model, the Competing Values Framework (Quinn & Rohrbaugh 1983). Three cooperatives were studied. Their presidents were interviewed, and a questionnaire was applied to cooperative members to obtain demographic and organizational culture data. Differences between the cooperatives’ cultural profiles seem to be consistent with both the circumstances of Portuguese social economy organizations (SEOs), and to the organizations’ uniqueness regarding their trade, focuses, and history. International firm trends were compared with this study’s results, and also appear to be explained by the SEO’s management practices evolution standpoint: lack of structured way of working, and the need to improvise and innovate in order to get things done. The importance of our research is held in the fact that social economy, and the cooperative movement in particular, has a developing importance in the expansion of many economies, the lack of literature on culture in SEOs, and the exploratory usage of a well-known model of management literature in cooperatives.

Indications of carbapenem resistance evolution through heteroresistance as an intermediate stage in Acinetobacter baumannii after carbapenem administration

We describe an in vivo evolution of an antimicrobial profile from susceptibility to full-resistance to carbapenems, with heteroresistance as an intermediate stage, in an Acinetobacter baumannii strain. Heteroresistance was characterized by the growth of sub-populations within the susceptibility halo in both disk-diffusion and Etest. PCRs for the main A. baumannii carbapenemases were negative. The exact resistance mechanism, diagnostic methods and clinical relevance of heteroresistance in A. baumannii warrant further investigations. This is the first description of such phenomenon in vivo and the second report of heteroresistance to carbapenems in A. baumannii.

Organizational culture in cooperatives: an exploratory approach

In recent years, organizational culture has become one of the common themes of interest of scientific and academic research. Each organization has its own unique cultural identity. Based on the recognition that organizational culture is considered important to an organization’s results, and social economy organizations are concerned with improving managerial practices and results, our objective is to study organizational culture in cooperatives: identifying their organizational culture as a specific type of organization of the social economy, recognized as increasingly important economic agents; and in doing so, explore the usage of a widely known model, the Competing Values Framework (Quinn & Rohrbaugh 1983). Three cooperatives were studied. Their presidents were interviewed, and a questionnaire was applied to cooperative members to obtain demographic and organizational culture data. Differences between the cooperatives’ cultural profiles seem to be consistent with both the circumstances of Portuguese social economy organizations (SEOs), and to the organizations’ uniqueness regarding their trade, focuses, and history. International firm trends were compared with this study’s results, and also appear to be explained by the SEO’s management practices evolution standpoint: lack of structured way of working, and the need to improvise and innovate in order to get things done. The importance of our research is held in the fact that social economy, and the cooperative movement in particular, has a developing importance in the expansion of many economies, the lack of literature on culture in SEOs, and the exploratory usage of a well-known model of management literature in cooperatives.

Transplantation in Highly Sensitised Patients Treated with Intravenous Immunoglobulin and Rituximab

Renal transplant in highly sensitised patients is associated with increased morbidity. The aim of this retrospective study was to evaluate the clinical evolution of 30 highly sensitised deceased donor kidney transplants and the influence of different timing of B cell directed treatment and its importance in the outcome of these patients. All recipients had negative complement dependent lymphocytotoxicity cytotoxic T cell crossmatch and no identified anti human leucocyte antigen class I donor specific antibodies. T cell flow crossmatch was performed within 24h of transplantation with serum obtained pretransplant (historic, recent or baseline). Posttransplant flow crossmatch were performed prospectively starting on the 3rd posttransplantation day. The immunosuppressive regime included thymoglobulin, tacrolimus, mycofenolate mofetil and steroids. Positive flow crossmatch occurred in 20/29 patients by the 3rd posttransplantation day, and in 17/27 patients after the 3rd posttransplantation day. All patients were started on intravenous immunoglobulin before transplantation: in nine patients (group A) at 400mg/kg/day for five days; in the remaining 21 patients (group B), as a continued infusion of 2g/kg during 48h. In group A, Rituximab was added only in the presence of antibody mediated rejection; in group B, introduced on the 3rd posttransplantation day whenever a positive flow crossmatch (with serum obtained pre or posttransplant) was reported. Antibody mediated rejection was observed in 44.4% of patients in group A, and 19% of those in group B. Mean follow-up was 12.2±5.5 months. Overall allograft survival was 76.6%, 81% in group B, and 66.6% in group A. At last follow up, mean serum creatinine was 1.3±0.6 mg/dl. Renal transplantation with pretransplant positive flow crossmatch is highly associated with antibody mediated rejection, despite introduction of intravenous immunoglobulin pretransplantation. However high dose intravenous immunoglobulin for 48h plus Rituximab by the 3rd posttransplantation day reduce the incidence of antibody mediated rejection by more than 50% and allowed for allograft survival of 81% at one year, with an excellent renal function.

EVOLUTION OF PATIENTS WITH AIDS AFTER cART: CLINICAL AND LABORATORY EVOLUTION OF PATIENTS WITH AIDS AFTER 48 WEEKS OF ANTIRETROVIRAL TREATMENT

SUMMARY Combination Antiretroviral Therapy (cART) aims to inhibit viral replication, delay immunodeficiency progression and improve survival in AIDS patients. The objective of this study was to compare two different schemes of cART, based on plasma viral load (VL) and CD4+ T lymphocyte count, during 48 weeks of treatment. For this purpose, 472 medical charts of a Specialized Outpatient Service were reviewed from 1998 to 2005. Out of these, 58 AIDS patients who had received a triple drug scheme as the initial treatment were included in the study and two groups were formed: Group 1 (G1): 47 individuals treated with two nucleoside reverse-transcriptase inhibitors (NRTI) and one non-nucleoside reverse-transcriptase inhibitor; Group 2 (G2): 11 patients treated with two NRTI and one protease inhibitor. In G1 and G2, 53.2% and 81.8% respectively were patients with an AIDS-defining disease. The T CD4+ lymphocyte count increased progressively up until the 24th week of treatment in all patients, while VL became undetectable in 68.1% of G1 and in 63.6% of G2. The study concluded that the evolutions of laboratory tests were similar in the two treatment groups and that both presented a favorable clinical evolution.

BODY WEIGHT AS A DETERMINANT OF CLINICAL EVOLUTION IN HAMSTERS (Mesocricetus auratus) INFECTED WITH Leishmania (Viannia) panamensis

SUMMARY The clinical outcome of infection with Leishmania species of the subgenus Viannia in hamster model (Mesocricetus auratus) has shown to be different depending on experimental protocol. Body weight has been a relevant determinant of the clinical outcome of the infection in hamsters with visceral leishmaniasis but its importance as a clinical parameter in hamsters with cutaneous leishmaniasis is not known. In this study, the clinical evolution of infection with L. (V) panamensis was evaluated in juvenile and adult male hamsters during 11 weeks by comparing clinical parameters such as attitude, temperature, respiratory rate, appearance of the stool, and body weight between infected and non-infected groups. Results showed that body weight decreased in adult hamsters after infection by L. (V) panamensis; this observation supports the use of body weight as an additional parameter to define the management or treatment of cutaneous leishmaniasis in infected adult hamsters used as an animal experimental model for leishmaniasis.

Aplicação das FPAA na realização de sistemas de ordem fraccionária

Esta tese de dissertação tem como principal objetivo a implementação de controladores fracionários utilizando diapositivos analógicos FPAA (Field Programable Analog Array). Embora estes dispositivos já não sejam um tecnologia recente, não tiveram grande aceitação comercial, daí não ter sido grande a sua evolução nesta última década. Mas para a elaboração de alguns circuitos analógicos, nomeadamente filtros, amplificadores e mesmo controladores PID (Proporcional-Integrativo-Derivativo) analógicos torna-se numa ferramenta que pode facilitar o projeto e implementação. Para a realização deste estudo, utilizou-se a placa de desenvolvimento da Anadigm AN231K04-DVLP3 juntamente com o software disponibilizado pela mesma empresa, o AnadigmDesigner2. Para a simulação e observação dos resultados foi utilizada a DAQ (Data Acquisition) Hilink da Zelton juntamente com o software Matlab. De forma a testar a implementação dos controladores fracionários nas FPAA foram realizados alguns circuitos no software e enviados para a FPAA comparando os resultados obtidos na simulação com os visualizados no osciloscópio. Por último foi projetado um controlador PIlDm recorrendo aos métodos de aproximação inteira descritos neste documento implementados na FPAA recorrendo ao uso de filtros de primeira e segunda ordem.

Criação e atualização de aplicações para área de retalho por MDA

Nos últimos anos tem-se verificado a constante evolução dos mercados em plataformas na Internet como forma de melhoria não só dos serviços prestados, mas também para o aumento de vendas de produtos e respetiva internacionalização dos mesmos. Este aumento da procura por este tipo de softwares, assim como a constante evolução e atualização dos mesmos tem contribuído para que estas aplicações evoluam em termos de funcionalidades e complexidade. Isto contribui cada vez para a dificuldade de formar equipas capazes de manter e desenvolver estes sistemas sem que comprometa em grandes custos para as organizações. Neste sentido surgiram diversas ferramentas que permitem criar soluções pré desenvolvidas de aplicações na Internet denominadas de "E-commerce applications". Estas plataformas, apesar do utilizador não ter obrigatoriamente que deter conhecimentos de programação para proceder à sua instalação, são bastante restritas tanto aos serviços que podem ser usados, e na sua escalabilidade, visto que normalmente correm em servidores específicos e por vezes as configurações necessárias para instalação tornam-se bastante complexas de ser efetuadas. Pretende-se no âmbito desta dissertação de mestrado propor um modelo de uma arquitetura de um sistema baseado em mecanismos MDA para a área de retalho, particularmente em ambientes de e-commerce. Serão inicialmente sistematizados os principais tipos de ecommerce numa perspetiva de evolução histórica. Será igualmente enquadrado o MDA no desenvolvimento de um sistema de e-commerce. Neste sentido, serão equacionadas as diferenças entre o modelo típico de desenvolvimento de software e o desenvolvimento de software orientado pelas metodologias do MDA. No processo de especificação e desenvolvimento do modelo proposto será realizada uma análise de requisitos, assim como, a proposta do modelo da arquitetura de um sistema baseado em mecanismos MDA, tendo como orientação os requisitos e arquitetura definida na fase de análise. Finalmente no sentido de analisar o resultado esperado para um sistema orientado por metodologias definidas por MDA, serão realizado alguns testes no sistema desenvolvido de forma a analisar o seu desempenho e validar a sua adequabilidade no âmbito do processo de desenvolvimento de sistemas e-commerce

Spatial and temporal analysis of recent drought using vegetation temperature condition index: case of Somali regional state of Ethiopia

Dissertation submitted in partial fulfillment of the requirements for the Degree of Master of Science in Geospatial Technologies.

The Role of the Humoral Immune Response in the Molecular Evolution of the Envelope C2, V3 and C3 Regions in Chronically HIV-2 Infected Patients

BACKGROUND: This study was designed to investigate, for the first time, the short-term molecular evolution of the HIV-2 C2, V3 and C3 envelope regions and its association with the immune response. Clonal sequences of the env C2V3C3 region were obtained from a cohort of eighteen HIV-2 chronically infected patients followed prospectively during 2-4 years. Genetic diversity, divergence, positive selection and glycosylation in the C2V3C3 region were analysed as a function of the number of CD4+ T cells and the anti-C2V3C3 IgG and IgA antibody reactivity RESULTS: The mean intra-host nucleotide diversity was 2.1% (SD, 1.1%), increasing along the course of infection in most patients. Diversity at the amino acid level was significantly lower for the V3 region and higher for the C2 region. The average divergence rate was 0.014 substitutions/site/year, which is similar to that reported in chronic HIV-1 infection. The number and position of positively selected sites was highly variable, except for codons 267 and 270 in C2 that were under strong and persistent positive selection in most patients. N-glycosylation sites located in C2 and V3 were conserved in all patients along the course of infection. Intra-host variation of C2V3C3-specific IgG response over time was inversely associated with the variation in nucleotide and amino acid diversity of the C2V3C3 region. Variation of the C2V3C3-specific IgA response was inversely associated with variation in the number of N-glycosylation sites. CONCLUSION: The evolutionary dynamics of HIV-2 envelope during chronic aviremic infection is similar to HIV-1 implying that the virus should be actively replicating in cellular compartments. Convergent evolution of N-glycosylation in C2 and V3, and the limited diversification of V3, indicates that there are important functional constraints to the potential diversity of the HIV-2 envelope. C2V3C3-specific IgG antibodies are effective at reducing viral population size limiting the number of virus escape mutants. The C3 region seems to be a target for IgA antibodies and increasing N-linked glycosylation may prevent HIV-2 envelope recognition by these antibodies. Our results provide new insights into the biology of HIV-2 and its relation with the human host and may have important implications for vaccine design.

Proposta de solução de videovigilância para locais remotos

Desde tempos remotos que homens faziam a vigilância de bens e mercadorias e mais recentemente também de pessoas com o intuito de dissuadir roubos, atos de vandalismo e de violência. Nos últimos anos, com a evolução das novas tecnologias verificou-se a sua adoção para auxílio da vigilância. Os atos de terrorismo que têm acontecido um pouco por todo o mundo trouxeram um clima de insegurança à população mundial. Este fenómeno, juntamente com o elevado número de roubos e atos de violência levou à expansão de utilização dos meios de videovigilância de forma a dissuadir estes tipos de crime podendo mesmo, nalguns casos servir como prova para punir os autores dos mesmos. Em Portugal tem-se verificado uma escalada de crimes nas zonas mais rurais não só de bens como as alfaias agrícolas mas também de frutos e mesmo de animais. Estes crimes predominam em locais rurais, relativamente distantes das povoações e em locais onde não existem (ou são praticamente inexistentes) infraestruturas necessárias para implementar meios de videovigilância como a falta de rede elétrica e internet o que torna quase inviável a existência de sistemas de videovigilância nesses locais. Dotar esses locais das infra estruturas necessárias poderia tornar-se demasiado dispendioso e os vigilantes humanos poderiam correr riscos no meio dos montes ou noutros locais remotos para além dos seus elevados custos. Para além do problema dos roubos, existe um outro flagelo relacionado com os incêndios na floresta portuguesa, que todos os anos é dizimada pelo fogo devido a incêndios que surgem na sua maioria causados pelo homem sendo uma parte significativa os de origem criminosa. Para dar resposta a estes problemas e no sentido de vigiar e dissuadir estes tipos de crimes, iniciamos um estudo que pretende propor um protótipo de um sistema de videovigilância para locais remotos (SVR - Sistema de Videovigilância Remota) de baixo custo de forma a diminuir o número de crimes e assim minimizar os prejuízos económico e sociais causados pelos mesmos. Pretendemos estudar o problema e analisar tecnologias com potencial para propor uma solução que possa auxiliar a vigilância nesse tipo de locais com o pressuposto de poder vir a contribuir para a diminuição deste tipo de crimes devido ao seu efeito dissuasor pelo facto de se poder divulgar que estes locais já têm uma solução de vigilância oculta. A solução proposta contempla um sistema de videovigilância com uma camara construída com base num Raspberry Pi onde o vídeo é transmitido em streaming via Web através de comunicações móveis. A alimentação do sistema nestes espaços sem energia elétrica é feita através de um painel fotovoltaico. É proporcionado ao utilizador uma interface para visualizar o vídeo transmitido e um mecanismo de notificações por email. É ainda possível a visualização de imagens gravadas num cartão de memória relativas a ocorrências de deteção de movimentos. Foram realizados inúmeros testes ao protótipo SVR sendo os resultados obtidos aqui descritos.

Evolution of the Human Immunodeficiency Virus Type 2 Envelope in the First Years of Infection is Associated with the Dynamics of the Neutralizing Antibody Response

Background: Differently from HIV-1, HIV-2 disease progression usually takes decades without antiretroviral therapy and the majority of HIV-2 infected individuals survive as elite controllers with normal CD4+ T cell counts and low or undetectable plasma viral load. Neutralizing antibodies (Nabs) are thought to play a central role in HIV-2 evolution and pathogenesis. However, the dynamic of the Nab response and resulting HIV-2 escape during acute infection and their impact in HIV-2 evolution and disease progression remain largely unknown. Our objective was to characterize the Nab response and the molecular and phenotypic evolution of HIV-2 in association with Nab escape in the first years of infection in two children infected at birth. Results: CD4+ T cells decreased from about 50% to below 30% in both children in the first five years of infection and the infecting R5 viruses were replaced by X4 viruses within the same period. With antiretroviral therapy, viral load in child 1 decreased to undetectable levels and CD4+ T cells recovered to normal levels, which have been sustained at least until the age of 12. In contrast, viral load increased in child 2 and she progressed to AIDS and death at age 9. Beginning in the first year of life, child 1 raised high titers of antibodies that neutralized primary R5 isolates more effectively than X4 isolates, both autologous and heterologous. Child 2 raised a weak X4-specific Nab response that decreased sharply as disease progressed. Rate of evolution, nucleotide and amino acid diversity, and positive selection, were significantly higher in the envelope of child 1 compared to child 2. Rates of R5-to-X4 tropism switch, of V1 and V3 sequence diversification, and of convergence of V3 to a β-hairpin structure were related with rate of escape from the neutralizing antibodies. Conclusion: Our data suggests that the molecular and phenotypic evolution of the human immunodeficiency virus type 2 envelope are related with the dynamics of the neutralizing antibody response providing further support for a model in which Nabs play an important role in HIV-2 pathogenesis.

Chromosomal structure: a selectable trait for evolution

Dissertation presented to obtain the Ph.D degree in Evolutionary Biology