Acute toxicity of beta gamma-CAT, a naturally existing non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions

In vertebrates, non-lens beta gamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained

无指盘臭蛙抗微生物多肽及其基因和在制药上的应用

本发明涉及一种无指盘臭蛙抗微生物多肽及其基因和在制药上的应用，属于生物医学领域。无指盘臭蛙抗微生物多肽是无指盘臭蛙抗微生物多肽基因编码的一种单链多肽，分子量为4248.27道尔顿，等电点为11.47，多肽全序列一级结构为：Gly Leu Phe Thr Leu Ile Lys Gly Ala Ala Lys Leu Ile Gly Lys Thr Val Pro Lys Lys Gln Ala Arg Leu Gly Met Asn Leu Trp Leu Val Lys Leu Pro Thr Asn Val Lys Thr (GLFTLIKGAAKLIGKTVPKKQARLGMNLWLVKLPTNVKT)。编码无指盘臭蛙抗微生物多肽的基因由343个核苷酸组成，其中编码成熟无指盘臭蛙抗微生物多肽为第123-240位核苷酸。人工合成的无指盘臭蛙抗微生物多肽具有很强的抑制细菌和真菌生长的作用，可以作为制备病原微生物感染疾病的治疗药物被应用。本发明的无指盘臭蛙抗微生物多肽具有抗菌谱系广、抗菌活性强的特点。

The First Gene-encoded Amphibian Neurotoxin

Many gene-encoded neurotoxins with various functions have been discovered in fish, reptiles, and mammals. A novel 60-residue neurotoxin peptide (anntoxin) that inhibited tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channel (VGSC) was purified and c

Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma

Semenogelin I (SgI) is one of the most abundant proteins in human seminal plasma. SgI plays a key role in sperm coagulation and spermatozoon immobilization. in addition, SgI and/or its proteolytic fragments are involved in regulating spermatozoon motility

无指盘臭蛙分泌肽及其基因和在制药中的应用

本发明涉及一种无指盘臭蛙分泌肽及其基因和在制药中的应用，属于生物医学领域。该活性多肽是从中国两栖类动物无指盘臭蛙基因编码的一种环状多肽，分子量1587.98道尔顿，等电点9.7，多肽全序列一级结构为： Phe Met Pro Ile Leu Ser Cys Ser Arg Phe Lys Arg Cys，其第七位半胱氨酸和第十三位半胱氨酸相成分子内二硫键。编码无指盘臭蛙分泌肽的基因由300个核苷酸组成，其中编码成熟无指盘臭蛙分泌肽为第138-177位核苷酸。人工合成的无指盘臭蛙分泌肽具有显著的抑制细菌和真菌生长的作用，可以作为制备病原微生物感染疾病的治疗药物被应用。本发明的无指盘臭蛙分泌肽具有结构简单、人工合成方便、抗菌谱系广的有益特点。

Purification, characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora

While investigating the innate defense of brackish water-living amphibian and its comparison with freshwater-living amphibians, two novel 12-residue antimicrobial peptides were purified from the skin secretions of the crab-eating frog, Fejervarya cancrivo

蛇毒产量与相关因素的测试观察

报道了我国常见毒蛇的采毒量及不同月份的产量。探讨取毒时间、喂食与蛇 毒产量、质量间的相互关系。研究结果表明,取毒时间以间隔20—30d取 一次最 好、不仅产量高而且活力较强,饥饿情况下蛇毒产量高、质量好, 食物投放最好 在取毒后, 每月或20d投食1—2次, 喂食频繁则对产量、质量均有影响。蛇毒毒 素性以3—4月或10—11月最佳, 6—9月毒力低。表4参4

金环蛇抗菌肽cathelicidin-BF及其基因和应用

一种金环蛇抗菌肽cathelicidin-BF及其基因和应用，属生物医学领域。 Cathelicidin-BF是一种直链多肽，含有三十个氨基酸残基，分子量3637.54Da，等电点11.79。cathelicidin-BF全序列为：赖氨酸-苯丙氨酸-苯丙氨酸-精氨酸-赖氨酸-亮氨酸-赖氨酸-赖氨酸-丝氨酸-缬氨酸-赖氨酸-赖氨酸 -精氨酸-丙氨酸-赖氨酸-谷氨酸-苯丙氨酸-苯丙氨酸-赖氨酸-赖氨酸 -脯氨酸-精氨酸-缬氨酸-异亮氨酸-甘氨酸-缬氨酸-丝氨酸-异亮氨酸 -脯氨酸-苯丙氨酸。编码cathelicidin-BF的基因由750个核苷酸组成，其中编码成熟肽部分的为第484-573位核苷酸。Cathelicidin-BF分子量小、杀菌作用强、作用时间迅速、对多种临床耐药菌有非常强的杀灭作用。此外还具有广谱抗菌和非盐依赖等有益特点。

Bacillus solisalsi sp nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake

A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1(T), was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at

眼镜蛇(Naja naja atra)毒膜毒素MT-I的生物学效应研究

从眼镜蛇毒中纯化出膜毒素MT-I,该毒素与磷脂酶A_(2)产生协同溶血效应,使小鸡颈二腹肌等标本产生进行性挛缩,对体外Hut-78细胞有杀伤作用,腹腔注射能引起家猫的心电图异常。

金环蛇抗菌肽cathelicidin-BF变异体cathelicidin-BF15及其应用

本发明涉及一种金环蛇抗菌肽cathelicidin-BF变异体cathelicidin- BF15及其应用，属于生物医学领域。金环蛇抗菌肽cathelicidin-BF变异体 cathelicidin-BF15是一种直链多肽，含有15个氨基酸残基，分子量1939.47Da，等电点12.05。cathelicidin-BF15全序列为：缬氨酸-赖氨酸-精氨酸-苯丙氨酸-赖氨酸-赖氨酸-苯丙氨酸-苯丙氨酸-精氨酸-赖氨酸-亮氨酸-赖氨酸-赖氨酸-丝氨酸-缬氨酸。cathelicidin-BF15具有强烈的杀菌作用，对多种临床耐药菌有非常强的杀灭作用，此外具有结构简单，人工合成方便和广谱抗菌等有益特点。

Saccharibacillus kuerlensis sp nov., isolated from a desert soil

A taxonomic study was performed on strain HR1(T), which was isolated from a desert soil sample collected from Xinjiang Province (China). Cells were aerobic, Gram-positive-staining, pink-pigmented, sporulating rods with a single lateral flagellum. The orga

Gene admixture in the Silk Road region of China: Evidence from mtDNA and melanocortin 1 receptor polymorphism

Mitochondrial DNA control region segment I sequences and melanocortin 1 receptor (MC1R) gene polymorphism were examined in ethnic populations in the silk road region of China. Both the frequencies of the MC1R variants and the results of mtDNA data in this region presented intermediate values between those of Europe and East and Southeast Asia, which suggested extensive gene admixture in this area and was in general agreement with previous studies. Phylogenetic analysis of the ethnic populations in the Silk Road region that based on mtDNA data didn't show expected cluster pattern according to their ethnogenesis. We suspect that a high migration rate in female among these closely related populations and other three demographic events might account for it.

滇蛙抗氧化肽及其基因和应用

本发明涉及一种滇蛙抗氧化肽及其基因和应用，属于生物医学技术领域。滇蛙抗氧化肽是中国两栖类动物滇蛙基因编码的一种单链多肽，分子量为1653.88 道尔顿，等电点8.22，滇蛙抗氧化肽全序列为：NH2-GIRPTYNRQCEIGF-COOH。编码的基因由316个核苷酸组成，其中编码成熟部分的为第130-171位核苷酸。人工合成的滇蛙抗氧化肽具有强烈的抗氧化活性，能作为制备皮肤抗氧化保护和体内自由基清除药物的应用，并且还具有序列简单、合成方便的优点。

Molecular and functional characterization of a c-type lysozyme from the Asian corn borer, Ostrinia furnacalis

Some lepidopteran lysozymes have been reported to display activity against Gram-positive and Gram-negative bacteria, in contrast to most lysozymes that are active only against Gram-positive bacteria. OstrinLysC, a c-type lysozyme, was purified from the As