955 resultados para Prospecção clonal


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In the wake of current global image involving environmental impacts, the use of wind power has had a remarkable growth in recent years as a technique for generating electricity. In fact, it is a source featuring strong dissemination of technology which provides decrease in costs and a greater access to low-income electricity. PROINFA (Incentive Program for Alternative Energy Sources) promotes a greater diffusion of new technologies for power generation, in particular wind-produced. Due to such a scenario on the exploitation of such energy source, current analysis discusses strategies for the development of domestic wind technology and the implications for electricity-lacking rural areas. Analysis shows a similar behavior between rural populations lacking electricity and the amount of potential energy available in the region. It is expected that this assay will contribute towards the establishment of public policies for wind-energy parks on rural farms in the North and Northeast regions of Brazil.

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This is the first report on the parasitoid Palmistichus elaeisis, genus Eulophidae, found in the field parasitizing pupae of defoliating eucalyptus. Lepidopterous pests occur in eucalyptus plantations in Brazil, reaching high population levels. Due to the complexity of pest control in eucalyptus forests, alternative control methods have been proposed, for instance biological control through use of parasitoids. Natural enemies play an important role in regulating host populations because their larvae feed on the eggs, larvae, pupae or adults of other insects. The parasitic Hymenoptera are important agents in biological control programs against forest pests, and may provide economic and environmental benefits. The generalist endoparasitoid Palmistichus elaeisis Delvare and LaSalle, 1993 (Hymenoptera: Eulophidae) can develop in its host’s pupae, which overcome the host’s physiology and can therefore be used for biological control of agricultural and forest pests. This study aimed to evaluate the impact of P. elaeisis as a pupal parasitoid of S. violascens in providing a potential alternative to chemical control of the pest and creation of an alternative host. The experiment was developed in the Laboratory for Biological Control of Forest Pests, Universidade Estadual Paulista "Julio Mesquita Filho”. Parasitoids used in this test were originally collected on pupae of E. eucerus. (Lepidoptera: Riodinidae) in eucalyptus plantations at Lençois Paulistas, São Paulo state, Brazil, in 2011. Thereafter, a laboratory culture has been maintained, using pupae of Spodoptera frugiperda (J. E. Smith) (Lep.: Noctuidae) as hosts. S. violascens eggs were collected in a eucalyptus clonal plantation in Sao Paulo state (Brazil). Larvae were reared under ambient conditions on Eucalyptus urophylla S.T. Blake (Myrtaceae) leaves. The following parameters were determined: parasitism level, numbers of emerged and non-emerged parasitoids and duration of egg-adult cycle. The S. violascens pupae were dissected to evaluate the non-emerged parasitoids. The parasitism level reached 100%, with a 100% emergence rate. It was verified that 113.2±0.8 parasitoids emerged per individual pupa versus only 0.7±0.1 that did not emerge. The P. elaeisis egg-adult cycle was 20.3±0.6 days in S. violascens pupae. This opens new perspectives for utilizing this parasitoid in biological control programs against caterpillars important to forestry. Sarsina violascens in Brazil.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Geociências e Meio Ambiente - IGCE

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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In the past few years, the Brazilian oil and gas sector had a considered growth, gaining enough relevance at international level, and being extremely important for the Brazilian socioeconomic development. However, the sector is still limited due to technical deficiencies. This work aims to conduct a current study, comprehending the entire oil chain, since it is possible to mention the Brazilian market updates on this context. To support this study, the historical development of the Brazilian petroleum industry was presented and the main stages of the production process were exposed with an emphasis on exploration and production phases. The new technologies developed for deepwater extractions, such as the pre-salt regions, were also discussed and, in the end, the relation of this growing market with the opportunities of the Human Resources Program of n. 48 was highlighted. This program identifies the market's actual needs and it's scarce's areas with the purpose of building capacity and qualified workforces, since it is also aimed, in a national level, a self-sufficient human resources for the sector

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In the past few years, the Brazilian oil and gas sector had a considered growth, gaining enough relevance at international level, and being extremely important for the Brazilian socioeconomic development. However, the sector is still limited due to technical deficiencies. This work aims to conduct a current study, comprehending the entire oil chain, since it is possible to mention the Brazilian market updates on this context. To support this study, the historical development of the Brazilian petroleum industry was presented and the main stages of the production process were exposed with an emphasis on exploration and production phases. The new technologies developed for deepwater extractions, such as the pre-salt regions, were also discussed and, in the end, the relation of this growing market with the opportunities of the Human Resources Program of n. 48 was highlighted. This program identifies the market's actual needs and it's scarce's areas with the purpose of building capacity and qualified workforces, since it is also aimed, in a national level, a self-sufficient human resources for the sector

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This paper chronicles a 2-year-old girl who presented with acute leukemia/lymphoma syndrome of the T cell immuno-phenotype. At this time, the cytogenetic analysis of her bone marrow cells showed a reciprocal translocation between the short arm of chromosome 12 and the long arm of chromosome 13, t(12;13)(p13;q14). The immunophenotyping of bone marrow blast cells by flow cytometry revealed a population of cells positive for CD56, CD117, CD45, partial CD33, partial HLA-DR, CD13, CD7, CD2 and CD5. Therefore, a diagnosis of acute leukemia with a mixed T cell/myeloid phenotype was made. The patient had a poor response to classic T cell acute lymphocytic leukemia/lymphoma therapy; thus, her treatment was changed to a myeloid leukemia protocol, which produced a good response. She underwent a successful cord blood transplantation from an unrelated HLA partially matched donor. The coexistence of these two phenotypes prompts questions about the existence of clonal instability, which might influence the choice of therapy. The rarity of the t(12;13)(p13;q14) and the coexistence of T cell/myeloid markers suggest a nonrandom association. To the best of our knowledge, this is the first reported case in which a cell clone bearing a t(12;13)(p13;q14) translocation in a mixed T cell/myeloid lesion was detected. Copyright (C) 2012 S. Karger AG, Basel

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Clonal eucalyptus plantings have increased in recent years; however, some clones with high production characteristics have vegetative propagation problems because of weak root and aerial development. Endophytic microorganisms live inside healthy plants without causing any damage to their hosts and can be beneficial, acting as plant growth promoters. We isolated endophytic bacteria from eucalyptus plants and evaluated their potential in plant growth promotion of clonal plantlets of Eucalyptus urophylla x E. grandis, known as the hybrid, E. urograndis. Eighteen isolates of E. urograndis, clone 4622, were tested for plant growth promotion using the same clone. These isolates were also evaluated for indole acetic acid production and their potential for nitrogen fixation and phosphate solubilization. The isolates were identified by partial sequencing of 16S rRNA. Bacillus subtilis was the most prevalent species. Several Bacillus species, including B. licheniformis and B. subtilis, were found for the first time as endophytes of eucalyptus. Bacillus sp strain EUCB 10 significantly increased the growth of the root and aerial parts of eucalyptus plantlets under greenhouse conditions, during the summer and winter seasons.

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Human T-cell lymphotropic virus type 1 (HTLV-1) is-an RNA virus responsible for diseases such as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukemia/lymphoma (ATL). Cell-to-cell contact and Tax-induced clonal expansion of infected cells are the main modes of virus replication, making virus detection during the viremic stage difficult. Consequently, the proviral load is the current virologic marker for disease monitoring, but the mechanisms of progression have not been established yet. Thus, this study investigated the presence of virus in plasma from asymptomatic HTLV-1 carriers and from HAM/TSP patients. Real-time PCR was performed on DNA from 150 plasma samples; 12(8%) had detectable DNA amplification, including 6(4%) asymptomatic HTLV-1 carriers and 14(26%) HAM/TSP patients (p < 0.005). Of the 33 samples submitted for nested PCR, six (18%, p = 0.02) were positive for HTLV-1 RNA in the plasma. Additionally, 26 plasma samples were treated with DNAse enzyme to eliminate any DNA contamination before RNA extraction. Two of them (8%) showed amplification for HTLV-1 (p = 0.5). Therefore, this study described for the first time the detection of free HTLV-1 RNA in plasma from HTLV-1-infected subjects, regardless of their clinical status. Thus, HTLV-1 viral replication does occur in plasma, and other transmission pathways for HTLV-1 should be investigated further. (C) 2011 Elsevier B.V. All rights reserved.