Studies on transportation of fish. Pt. 1. Packaging of frozen fish in insulated containers

The paper deals with certain technological aspects of transportation of fish. Frozen fish packed in thermocole insulated plywood box (tea chests) and transported has been found to remain in good condition, for 3 to 4 days. Fish reaching the destination in a thawed state can be stored in crushed ice for a further period of 2-3 days. The effect of initial temperature of frozen fish on the storage life, the maximum storage period for different types of frozen fish packed in the insulated container and the changes in chemical and organoleptic qualities of different varieties of fish under condition of transport are also discussed.

On the response of Catla catla (Ham) and Cirrhinus mrigala (Ham) to the voltage gradient of electrical field

The threshold body voltage (voltage gradient between head and tail) required for first reaction, electro-taxis and electro-narcosis depended upon species, conductivity of the fish body, nature of current and wave shape. Larger fishes showed first reaction at a lower body voltage than smaller ones. All the three reactions were dependent on the accommodation of nerves to the electrical field and subsequent fatigue of the fishes. No significant change was observed in the period of narcosis and recovery after repeated stimulation.

A few observations on the biology of Tachysurus thalassinus Ruppell from Veraval (Gujarat)

The length-weight relationship of T. thalassinus differed in the sexes and, therefore separate equations were obtained; for males log W = -5.1728 4- 3.0495 log L; and for females log W = -5.7456 + 3.2798 log L. The spawning period of this species appears to be restricted to a short period from October to November. Fecundity has been found to range from 33 to 55 mature eggs in specimens of the size range 421-564mm. The sex ratio of males to females was 1.8:1. T. thalassinus is a bottom carnivore, crustaceans ranking first in food followed by fishes, mollusc and polychaetes.

Field application of anabolic steroids in carp seed production. Pt. 1. Rearing of fry to fingerling stage

Ethylestrenol (17β Hydroxy-17alpha-ethyl-estr-4-en-3-one) and Stanozolal (17β-Hydroxy-17alpha-methyl 1-5 alpha-androstano-(3,2-C)-pyrazole), both synthetic androgenic steroids, were fed via diet at 3ppm to the fry of catla, rohu and silver carp which were reared up to fingerling stage over a period of 167-172 days in earthen ponds. Ethylestrenol enhanced growth in silver carp and rohu but retarded growth in catla. Stanozolal depressed growth in all the 3 species. Length-weight relationship for these fry had been worked out and the relative condition factor in all the cases was very close to or slightly above 1.0.

Limnology of Chanda beel

A limno-biological investigation was conducted in Chanda beel over a period of 8 months from June ‘95 to January ‘96. The floodplain showed temporal spatial and vertical variation in physico-chemical as well as biological conditions. During study period, physico-chemical parameters were within the suitable range for fish culture. Plankton population was higher in true beel areas. Both phytoplankton and zooplankton showed direct relationship among themselves. Presence of several indication plankton genera showed that the floodplain was eutrophic in nature.

Some aspects of association and development of Lytocestus indicus Moghe in catfish Clarias batrachus (Lin.)

Some aspects of association and development of the caryophyllid cestode Lytocestus indicus Moghe, 1925 infecting the catfish Clarias batrachus (Linn.) from the Kailla Beel of Mymensingh, Bangladesh were studied. About 33.14% of Clarias batrachus were infected with a mean intensity of 3.75, mean density 1.25. The infection was not found throughout the year. Two seasonal occurrence of this cestode were observed, one in April-May and the other in August-September. However, maturation period of the worm coincided with the maturation of the host. The worm was found attached to the wall of the intestine of the host. At the site of attachment tissue layers were compressed due to mechanical injuries. Prevalence and mean intensity of infection increased with length groups. No variation in infection was significantly observed in different sexes of the host examined.

Culture feasibility of african catfish (Clarias gariepinus Lin.) fry in glass tank and synthetic hapa system using supplemental diets

An experiment was carried out with 1 0 days old Clarias gariepinus fry over a period of 42 days to determine the effects of different feeds on growth and survival of African catfish fry in glass tanks. The experiment was designed into four treatments each having three replications. Thus treatment 1 (T1) was named as Tank Tubifex (TT) and treatment 2 (T2) as Tank Sabinco (TS), treatment 3 as Pond Tubifex (PT), and treatment 4 (T4) as Pond Sabinco (PS). Live Tubifex (protein levels 64.48%) was supplied to treatments 1 and 3 and rest of the treatments were supplied Sabinco starter-1 (protein levels 40.13%). The highest and the lowest growth in total length and weight were 12.90cm, 18.77g and 6.17cm, 4.04g recorded from the treatments 3 and 2, respectively. Growth of catfish fry under treatment 3 in terms of both length and weight were significantly higher (P<0.01) than those of the other treatments. However treatment 2 showed the significantly lowest (P<0.01) growth performance among the various treatments. The highest survival rate (92%) was also obtained with treatment 3. Tubifex proved to be the best larval feed in respect of growth and survival rate.

Effects of testosterone propionate on growth, survival and sex-ratio of African catfish (Clarias gariepinus Burchell)

For studying the effects of different levels of testosterone propionate on growth, survival and sex-ratio, five different doses such as 125, 100, 75, 50, 25 mg hormone per kg feed were administered to 5-day old Clarias gariepinus fry through diet for a period of 40 days. The growth performance in terms of weight and length gain of the fry receiving 100 and 75 mg hormone per kg feed were significantly higher than those receiving 50, 25 and 0 (untreated control) mg hormone per kg feed. The groups of fry treated with higher doses of hormone showed lower survival compared to those with lower doses of hormone. The frequency of male fish in the entire hormone treated groups except the 125mg/kg group, was significantly higher than that of the expected frequency of male fish in a normal population. The highest frequency of male fish, 92.08%, was obtained with the diet containing 50 mg hormone/kg diet however, the highest levels of hormone (125mg/kg diet) resulted in relatively lower frequency of male fish.

Rearing of Clarias batrachus (Lin.) larvae with formulated diets

An experiment was carried out for a period of 20 days using 7-day old Clarias batrachus larvae of initial total length of 7.4 ± 0.49 mm and weight of 2.9 ± 0.83 mg. Three artificial diets were used for the study having three replication of each. Among these, diet-I was formulated using 20% fish meal (FM), 30% powdered milk and 30% boiled egg yolk (BEY), diet-II using 27% FM, 20% Baker’s yeast (BY), 30% BEY and 3% agar and diet-III using 20% FM, 20% BY and 45% whole egg. The larvae fed on diets-II and III showed significantly (P<0.05) better length and weight gain than those of the larvae fed on diet-I. The larvae fed on diet- III showed the best survival rate (70%). However, the condition factor of the larvae fed on diet I was significantly better than those of the larvae fed on other two diets. The results of the study showed that C. batrachus larvae could be successfully reared with diet containing 45% whole egg, 20% yeast and 20% fish meal.

Gill pathology of juvenile carps in nursery ponds

Gill pathology of juveniles of two Indian major carps Labeo rohita and Cirrhinus mrigala were studied for a culture period of three months in a private and a government fish farm pond. Under histopathological observations, only protozoan parasite, Myxobolus sp., was recorded as cyst. These myxosporidian cysts were high in the gills of L. rohita of the government farm pond followed by C. mrigala of the private farm pond. Hypertrophic gill lamellae (primary and secondary) with loss of secondary lamellae were evidenced in C. mrigala of privately operated pond.

Studies on the induced breeding and post-larval rearing of shing (Heteropneustes fossilis Bloch)

An experiment was conducted on induced breeding and fry rearing of shing, Heteropneustes fossilis (Bloch) in the Department of Aquaculture, Bangladesh Agricultural University for a period of four months from April to July 1994. Hatching rate was calculated at 21.50h and was found to be 45 to 55 % and the survival rate of larvae was 30 to 40 % at 26 to 29°C. Survival rate and growth rate of post larvae were found to be 50 to 60% and 96.6 to 117.2% respectively. Feed-3 (F3) showed the highest survival rate and growth rate of post larvae.

Growth and survival of Clarias batrachus (Lin.) larvae fed on formulated diets

An experiment was conducted for a period of 28 days using 7-day old C. batrachus larvae of initial total length (±S.D.) of 7.4 ± 0.49 mm and weight (±S.D.) of 2.8 ± 0.75 mg. Five test diets viz. diets I, II, III, IV and V were prepared. Diet I was formulated using 30% fish meal (FM), 20% soybean meal (SM) and 20% Baker's yeast (BY), diet III using 15% FM, 20% SM, 15% cotton seed meal and 30% BY and diet IV using 20% FM, 30% BY and 30% powdered milk. The remaining two diets (diets II and V) were prepared using minced clam meat (96%) and BY (90%) respectively as the sole source of protein. Three replications were used for each treatment. The larvae fed on diet II exhibited significantly (P<0.05) better length gain and specific growth rate (SGR) than those of the larvae fed on diet III and there was no significant difference among the length gain and SGR of the larvae fed on diet I,II,IV and V. The larvae fed on diet II also showed significantly better weight gain and survival than those of the larvae fed on other diets. There was no significant difference between the condition factor of the larvae fed on diet II and IV. However, the larvae fed on diet II showed better condition factor than those of the larvae fed on other diets.

Effect of different rice brans on the growth of Thai silver barb (Puntius gonionotus Bleeker) in seasonal ponds

An experiment was conducted for a period of 110 days to study the effect of different rice brans on the growth of Thai silver barb (Puntius gonionotus, Bleeker) in rainfed seasonal ponds (30 m² each). Each of the ponds was stocked with 150 fish with the mean initial body weight of 2.03 ± 0.03 g. There were three treatments namely R1, R2 and R3 each having four replicates. Three types of rice bran namely coarse, auto (fine) and red were applied to the treatments R1, R2 and R3 respectively. Fish received different types of rice bran at a rate of 5% of body weight daily. The water quality parameters were found within the productive range. The results showed that treatment R1 produced significantly (p<0.05) highest growth and treatment R2 produced the lowest growth. The survival rates varied between 77 and 84% with treatment R3 producing the highest survival. However, the overall best production (1530 kg/ha) and economic return for the culture period was obtained in treatment R1 receiving coarse rice bran. The results of the present study demonstrated that the coarse rice bran resulted in better growth and production of P. gonionotus.

Effects of periphyton grown on bamboo substrates on growth and production of Indian major carp, rohu (Label rohita Ham.)

The effects of periphyton, grown on bamboo substrates, on growth and production of Indian major carp, rohu, Labeo rohita (Hamilton), were studied in 10 ponds during July to October '95 at the Bangladesh Agricultural University, Mymensingh. Five ponds were provided with bamboo substrates (treatment I) and the rests without bamboo substrates (treatment II). It was revealed that there had been no discernible difference in the water quality parameters between treatments. A large number of plankton (30 genera) showed periphytic nature and colonized on the bamboo substrates. The growth and production of fish was significantly (p<0.05) higher in the ponds with bamboo substrates as compared to the ponds without substrates. The net production of rohu in treatment I was about 1.7 times higher than that of treatment II. Fish production was as much as 1899 kg/ha over a culture period of 4 months in the periphyton-based production system.

Limnological conditions of naturally turbid ponds and its effects on fish growth

An experiment was conducted on six naturally turbid ponds in the village of Salakandi situated to the south-west corner of Bangladesh Agricultural University, Mymensingh for a period of three months from July to September '96. The experiment was performed by studying the physicochemical factors of water and soil, the biological factors such as densities of phytoplankton and zooplankton, and the growth of fishes. During the experimental period water turbidity varied among ponds. The highest value of turbidity was found to be 679±183.6 FTU in pond 1 and the lowest was 158±23.31 FTU in pond 4. The maximum and the minimum water transparency were recorded in the month of July and September respectably. The lowest net weight of fishes was found 28.8 kg/ha/year in pond 1 due to high turbidity and the highest was 35.8 kg/ha/year in pond 4 due to low turbidity. Most of the correlations between turbidity and transparency, phytoplankton, and zooplankton were significant at 1% and 5% levels.