984 resultados para MIÑO, REINALDO
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Muscle atrophy is always associated with Dexamethasone (Dexa) treatment, however the mechanisms are not completely understood. This study investigated the effects of Dexa on myostatin and p70S6K protein expression and if previous exercise training (T) can attenuate these effects. Eighty rats were distributed into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD; 0,5 mg/kg per day, i.p., 10 days), trained control (TC) and trained treated with Dexa (TD) and underwent a training period where they were either submitted to a running protocol (60% of physical capacity, 5 days/week for 8 weeks) or kept sedentary. After T period, animals underwent Dexa treatment concomitant with training. Western Blot was performed to identify myostatin and p70S6k protein expression in the tibialis anterior (TA) and soleus (SOL) muscle. Ten days of Dexa treatment increased fasting glucose (SD=+62%), however previous T attenuated this increase (TD=+20%, p<0.05). Dexa determined significant decrease in body weight in TD (-22%) and SD (-25%), followed by TA weight reduction in SD (-23%) and TD (-20%). Previous training could not avoid these decreases. Myostatin protein expression was not altered by dexa treatment or training in TA muscle but in SOL muscle it was significantly modified after T, regardless of treatment (TC=+%23 and TD=+25) compared with their respective controls. The protein p70S6K was not modified neither by dexa nor training in any of the analyzed muscle or condition. The results of this study allowed us to conclude that previous training attenuates the hyperglycemia induced by Dexa, however it did not prevent the body or muscle weight reductions. Even in the presence of muscle atrophy, the expression of myostatin and p70S6K do not justify the mechanisms of muscle loss induced by Dexa, which suggests that other catabolic or anabolic proteins could be involved in the process of muscle atrophy after 10 days of treatment with Dexa
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The purpose of the present study was to analyze the visual control of braking a bicycle when the cyclist is surprised by an obstacle in his way. According to Lee (1976), visually controlled braking based on time to collision information utilizes the optic variables tau and its first derivative in time, tau-dot, to initiating the braking action and regulating its intensity. Seven young adults performed a bicycle braking task in curvilinear trajectory under distinct velocity (high, medium, and low) and uncertainty (certainty and uncertainty) conditions. Results showed that, independently of velocity and uncertainty levels, participants utilized tau and tau-dot to initiating and regulating the braking action, avoiding collision with the obstacle. Cognitive, attentional, and other psychological factors resulting from both increased velocity and uncertainty were not capable of altering the use of time to collision information, corroborating the tested hypothesis
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This paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in Sa (a) over tildeo Paulo State, Brazil. The introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. Bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 positive animals, vaccinated and revaccinated, presented meningoencephalitis, thereby characterizing vaccinal failure.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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A new species of Myxosporea, Henneguya aequidens sp. n. (Myxozoa: Myxobolidae), was described based on its ultrastructural features. This is a parasite of the freshwater fish Aequidens plagiozonatus, in the Peixe-boi River, Para, Brazil. This parasite was found in the gills, in the form of whitish ellipsoid cysts with mature spores inside them. The average spore body was 15 +/- 0.9 mu m in length (n = 30) and 6 +/- 0.8 mu m in width (n = 30), and the tail measured 27 +/- 0.5 mu m in length (n = 15). The spores showed typical features of the genus Henneguya with two valves of equal size and two symmetrical polar capsules of 3 +/- 0.3 mu m in length and 2 +/- 0.3 mu m in width. Each polar capsule had a polar filament forming a helix from the apical region to the polar caps, with four to six turns. Based on the ultrastructural differences in morphology of these spores, the location of the parasite, and its host specificity, this parasite was described as a new species.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Experiments of continuous alcoholic fermentation of sugarcane juice with flocculating yeast recycle were conducted in a system of two 0.22-L tower bioreactors in series, operated at a range of dilution rates (D (1) = D (2) = 0.27-0.95 h(-1)), constant recycle ratio (alpha = F (R) /F = 4.0) and a sugar concentration in the feed stream (S (0)) around 150 g/L. The data obtained in these experimental conditions were used to adjust the parameters of a mathematical model previously developed for the single-stage process. This model considers each of the tower bioreactors as a perfectly mixed continuous reactor and the kinetics of cell growth and product formation takes into account the limitation by substrate and the inhibition by ethanol and biomass, as well as the substrate consumption for cellular maintenance. The model predictions agreed satisfactorily with the measurements taken in both stages of the cascade. The major differences with respect to the kinetic parameters previously estimated for a single-stage system were observed for the maximum specific growth rate, for the inhibition constants of cell growth and for the specific rate of substrate consumption for cell maintenance. Mathematical models were validated and used to simulate alternative operating conditions as well as to analyze the performance of the two-stage process against that of the single-stage process.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)