Efeito competicional em testes de progênies de eucalipto

Many studies have recommended the use of small plots for forest experiments, although they do not consider the inter-genotype competition increase. If this competition is not isolated from the mathematics model, it can lead to incorrect selection of genetic materials. The aim of this work was to evaluate the effect of seven competition covariates in two Eucalyptus spp. progeny tests. Data from the two half-sib eucalyptus progenies were analyzed, using the randomized blocks design. The seven analyzed covariates were HegyI's competition index (IC), self-competition (AT), alo competition (AL), self-competition mean (MAT), alo competition mean (MAL), and arithmetic means of four (M4) and eight (M8) nearest neighbors. Individual and combined analyses of covariates were used for the wood volume trait. All the variance components and the changes caused by covariates use were evaluated. The competition affects the results of eucalypt progeny analysis in different ways, according to its type, self or alo competition. Most influential covariates were MAT, MAL and IC. Most promising results of competition effects reduction were observed for the IC/MAT covariates inclusion in eucalypt progeny tests.

Variáveis epidemiológicas e alterações clínicas, hematológicas e urinárias em cães sororreagentes para Leptospira spp

In order to determine antibodies anti-Leptospira spp. in dogs from Botucatu-SP with a clinical suspicion and the risk factors for this zoonosis, as well possible hematological and urine alterations were collected blood samples from 248 dogs. The diagnostic test performed was the Microscopic Agglutination Test (MAT) with 29 serovars of leptospires. Titers were considered reagent ≥200 and detected in 17.7% (44/248) of the dogs. The most frequent serovars were Autumnalis (20.5%); Pyrogenes (18.2%); Gryppothyphosa (15.9%); Canicola (13.6%); Bratislava and Copenhageni (9.1%); Andamana (4.5%) and Djasiman (2.3%). Regarding the analysis of the epidemiological questionnaire administered to owners of the dogs, there was statistical significance for sex, age and presence of rats at home, showing that, by logistic regression analysis, only the sex variable was been significantly associated to the presence of antibodies anti-Leptospira. The levels of urea and creatinine were significantly increased in the group of animals reagents to the Microscopic Agglutination Test, showing the renal damage in these animals and decreased hemoglobin level.

Molecular and morphological data support the existence of a sexual cycle in species of the genus Paracoccidioides

The genus Paracoccidioides includes the thermodimorphic species Paracoccidioides brasiliensis and P. lutzii, both of which are etiologic agents of paracoccidioidomycosis, a systemic mycosis that affects humans in Latin America. Despite the common occurrence of a sexual stage among closely related fungi, this has not been observed with Paracoccidioides species, which have thus been considered asexual. Molecular evolutionary studies revealed recombination events within isolated populations of the genus Paracoccidioides, suggesting the possible existence of a sexual cycle. Comparative genomic analysis of all dimorphic fungi and Saccharomyces cerevisiae demonstrated the presence of conserved genes involved in sexual reproduction, including those encoding mating regulators such as MAT, pheromone receptors, pheromone-processing enzymes, and mating signaling regulators. The expression of sex-related genes in the yeast and mycelial phases of both Paracoccidioides species was also detected by realtime PCR, with nearly all of these genes being expressed preferentially in the filamentous form of the pathogens. In addition, the expression of sex-related genes was responsive to the putative presence of pheromone in the supernatants obtained from previous cocultures of strains of two different mating types. In vitro crossing of isolates of different mating types, discriminated by phylogenetic analysis of the α-box (MAT1-1) and the high-mobility-group (HMG) domain (MAT1-2), led to the identification of the formation of young ascocarps with constricted coiled hyphae related to the initial stage of mating. These genomic and morphological analyses strongly support the existence of a sexual cycle in species of the genus Paracoccidioides. © 2013, American Society for Microbiology.

Antagonist coactivation of trunk stabilizer muscles during Pilates exercises

The purpose of this study was to compare the antagonist coactivation of the local and global trunk muscles during mat-based exercises of Skilled Modern Pilates. Twelve women performed five exercises and concurrently, surface EMG from internal oblique (OI), multifidus (MU), rectus abdominis (RA) and iliocostalis lumborum (IL) muscles was recorded bilaterally. The percentage of antagonist coactivation between local (OI/MU) and global muscles (RA/IL) was calculated. Individuals new to the practice of these exercises showed differences in coactivation of the trunk muscles between the exercises and these results were not similar bilaterally. Thus, in clinical practice, the therapist should be aware of factors such as compensation and undesirable rotation movements of the trunk. Moreover, the coactivation of global muscles was higher bilaterally in all exercises analyzed. This suggests that the exercises of Skilled Modern Pilates only should be performed after appropriate learning and correct execution of all principles, mainly the Centering Principle. © 2013 Elsevier Ltd. All rights reserved.

Inquérito sorológico, molecular e fatores de risco para leptospirose em mamíferos cativos, papel dos animais sinantrópicos presentes no local e aspectos de saúde pública

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Subsídios para análise do ciclo de vida de assentos à base de fibra de coco e látex

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolamento e reação em cadeia pela polimerase (PCR) para Leptospira spp. em amostras renais e hepáticas de ovinos sorologicamente positivos e negativos para leptospirose, abatidos em frigorífico, procedentes do estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ocorrência de anticorpos e caracterização genotípica de isolados de Toxoplasma gondii em pombos (Zenaida auriculata) de vida livre capturados em Londrina, Paraná

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alterações espermáticas e dos níveis plasmáticos de testosterona em cães experimentalmente infectados por Leptospira interrogans sorovar Canicola

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Infecção Experimental com Leptospira interrogans sorovariedade Canicola, relacionada à pesquisa de alterações oculares em cães

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prevalência e fatores de risco de leptospirose bovina no estado do Maranhão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção de anticorpos para Toxoplasma gondii em soros de suínos de criações de fundo de quintal na microrregião de Registro-SP, pelo método de aglutinação direta

Pós-graduação em Medicina Veterinária - FMVZ

Comparação de técnicas no diagnóstico da infecção leptospírica em ovinos e implicações na saúde pública

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pesquisa de anticorpos anti-Leptospira spp, Toxoplasma gondii e Neospora caninum em cães recolhidos das ruas e albergados em canil privado de Avaré (SP)

Pós-graduação em Medicina Veterinária - FMVZ

Diferenciação entre os estágios agudo e crônico na infecção toxoplásmica pelo método de aglutinação direta modificado e pesquisa do agente no leite de ovelhas naturalmente infectadas por Toxoplasma gondii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)