Diversity of phytoseiid mites (Acari: Mesostigmata: Phytoseiidae) in the Atlantic Forest of São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Actinedida e Acaridida (Arachnida: Acari) edáficos de Piracicaba, estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Distribuição de Leptopharsa heveae em seringal no Estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Gamasida (Arachnida: Acari) Edáficos de Piracicaba, Estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Haplotype characterization of the COI mitochondrial gene in Chrysoperla externa (Neuroptera: Chrysopidae) from different environments in Jaboticabal, state of São Paulo, southeastern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Flutuação populacional e análise faunística de Carabidae e Staphylinidae (Coleoptera) em Jaboticabal, São Paulo

In this study a population survey of Carabidae and Staphylinidae (Coleoptera) adults was carried out with the aim to analyze their populations through faunistic indexes and to determine the influence of temperature, relative humidity and rainfall. The study was conducted in two areas cultivated with soybean/corn under no-tillage and conventional tillage systems and adjacent to a forest fragment and a Pinus stand, respectively. Beetles were sampled by pitfall traps distributed in two 100-m transects. The fauna was characterized by diversity (H), evenness (J), abundance, dominance, frequency and constancy indexes. The influence of air temperature, relative humidity and rainfall was verified using stepwise regression. The carabids Abaris basistriatus Chaudoir, Odontochila nodicornis (Dejean) and Scarites sp. were classified as predominant species in both areas, while Selenophorus alternans Dejean only classified as predominant in the area under conventional tillage system, likewise for Megacephala sp. and Selenophorus seriatoporus Putz. in the no-tillage area. The predominant species showed just one population peak each year, usually observed from December to January. The pluvial precipitation influenced the occurrence of the carabids positively and significantly, with O. nodicornis and Megacephala sp. being the most demanding species in relation to the effect of humidity on their dispersal.

Assinalamentos de Meloidogyne enterolobii em Goiabeira e em Plantas Invasoras no Estado de São Paulo, Brasil

Information regarding to the geographical distribution of Meloidogyne enterolobii in guava orchards situated in the State of São Paulo, Brazil, are included, with indication of the municipalities from where the nematode was recorded and data of average number of nematodes per 10 g roots. In addition, some weed species rated as new hosts of the guava root-knot nematode are listed.

First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil

Toxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of T. gondii for infection in humans. In the present study, we report the first isolation of viable T. gondii from finishing pigs in Brazil. Antibodies to T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically T. gondii isolates from pigs from Brazil are distinct from isolates of T gondii from pigs in the USA. (c) 2005 Elsevier B.V. All rights reserved.

Ocorrência de Staphylococcus sp. em água utilizada em propriedades leiteiras do Estado de São Paulo

Com o objetivo de verificar o papel da água utilizada durante a produção do leite como via de transmissão de Staphylococcus sp., fez-se a contagem de Staphylococcus coagulase negativa e Staphylococcus aureus nas amostras de água das fontes, dos reservatórios e dos estábulos de 30 propriedades leiteiras situadas na região Nordeste do Estado de São Paulo. As maiores ocorrências de isolamentos (10,0 e 16,6%) e os maiores valores médios (4,3×10(4) e 2,5×10(4)) de contagens desses microrganismos foram encontrados nas amostras de água dos estábulos utilizada na obtenção de leite. Estes resultados são importantes pois evidenciam a possibilidade de contaminação do leite ou dos animais por cepas patogênicas.

Rotavírus bovino: fatores de risco, prevalência e caracterização antigênica de amostras em rebanhos leiteiros no estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infecções por helmintos em Javalis (Sus scrofa scrofa) criados em cativeiro na região Noroeste do Estado de São Paulo, Brasil

O estudo foi desenvolvido no período de outubro de 2001 a maio de 2002, com 51 javalis (Sus scrofa scrofa) proveniente de duas propriedades situadas nos municípios de Mirassol e Fernandópolis, Estado de São Paulo, sendo 18 animais variando de 150 a 360 dias (Grupo I) e 33 animais entre 30 a 120 dias de vida (Grupo II). O objetivo da investigação foi identificar a helmintofauna e obter subsídio para melhor conhecimento da instalação das infecções helmínticas. Foram identificadas nos dois grupos, respectivamente, oito espécies de nematódeos com as seguintes prevalências: Trichuris suis (16,7% e 30,3%); Metastrongylus salmi (50,0% e 15,2%), Metastrongylus pudendotectus (5,6% e 3,0%); Strongyloides ransomi (27,8% e 12,1%); Ascaris suum (0,0% e 3,0%), Ascarops strongylina (27,8% e 0,0%); Physocephalus sexalatus (5,6% e 0,0%); Oesophagostomum dentatum (22,2% e 0,0%). O total de nematódeos colhidos e identificados nos dois grupos de animais foi de 7958, assim distribuídos: 6573 no intestino grosso (82,6%), 1246 no pulmão (15,7%), 89 no intestino delgado (1,1%), e 50 no estômago (0,6%). As maiores variações de intensidade foram obtidas por T. suis de 1 a 1764 e por M. salmi 1 a 248 exemplares.

Detection of antibodies against bovine respiratory syncytial virus (BRSV) in dairy cattle with different prevalences of bovine herpesvirus type 1 (BoHV-1) in São Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Abundância e sazonalidade de dípteros (Insecta) em granja aviária da região nordeste do Estado de São Paulo, Brasil

Modem production systems accommodate broody hens in high densities, leading to the accumulation of excrement under the cages. This substrate is excellent for the development of sinantropic flies. Thus, the accomplishment of surveys in these places becomes essential, in order to plan better strategies of control. The present work aimed at studying the entornofauna and the seasonality of the species of dipterous present in the Crisdan poultry house located in the Municipality of Sao Joao da Boa Vista, the State of São Paulo, Brazil. In the period of January of 2001 to December of 2002, 1,012,595 flies were captured using the "jug-trap". The species were identified: Drosophi-la repleta (Wollaston, 1858), Musca domestica (Linnaeus, 1758), Ophyra spp., Hennetria illucens (Linnaeus, 1758), Fannia canicularis (Linnaeus, 1761), Chrysomya megacephala (Fabricius, 1794), and Sepsidae. More frequently D. repleta and M. domestica had added 99.47% of the dipterous. Increased rainfall and the collection months influenced the sampling of dipterous (P < 0.05). Drosophila repleta was the most abundant species, representing 91% of all captured flies. However, this diptera did not develop at the surveyed site since immatures were not captured therein.

Brucellosis due to Brucella suis in a swine herd associated with a human clinical case in the State of São Paulo, Brazil

Brucella suis has been recognized as the major etiological agent of human brucellosis in areas free from Brucella melitensis infection. However, with changes in swine management, the occurrence of swine brucellosis has decreased as has the human incidence of B. suis infection. A swine brucellosis outbreak within a herd from Jaboticabal (So Paulo, Brazil) was detected in July 2006. The herd comprised approximately 300 sows and 1,500 finishing animals. Many sows within this herd experienced abortions, while others exhibited vaginal discharge; three sows suffered posterior paralysis. Among 271 sows, 254 (93.7%) tested positive for brucellosis by complement fixation, and among 62 randomly bled finishing animals, 17 (27.4%) also tested positive. The B. suis biovar 1 was cultured from 14 aborted fetuses and six sows. Brucella was identified using routine methods. Fourteen farm workers were tested using agglutination tests, with three workers showing evidence of Brucella antibody titers. A 39-year-old woman, who worked with maternal pigs and had direct contact with aborted fetuses, presented an agglutinating titer of 480 IU/mL and displayed clinical signs of infection. Our findings suggest that despite a reduction of swine brucellosis throughout Brazil, B. suis infection still occurs, thereby posing a zoonotic risk.

Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)