A Agricultura de Conservação como resposta ao desafio da gestão sustentável do solo e da água: Uma perspetiva europeia

Qual é a perspetiva europeia? Quais são os desafios? A PAC e a gestão sustentável do solo e da água Os “deliverables” da Agricultura de Conservação (AC) em relação à gestão sustentável do solo e da água, e não só! A Agricultura de Conservação na Europa e no Mundo A Agricultura de Conservação e a PAC Mensagens

Possibilities and challenges for No-till practices in Bulgaria

What is No-till? From No-till to Conservation Agriculture (CA) Why use No-till/CA? Where is No-till/CA practiced? The Bulgarian context The Ups The challenges No-till/CA in the context of CAP

Policy and Institutional Support for CA Development (Examples from Europe, Africa, Asia)

Policy and Institutional Support for CA Development (Examples from Europe, Africa, Asia)

Development and characterisation of a neurogenic model of brain cancer

Primary glioblastoma (GB), the most common and aggressive adult brain tumour, is refractory to conventional therapies and characterised by poor prognosis. GB displays striking cellular heterogeneity, with a sub-population, called Glioblastoma Stem Cells (GSCs), intrinsically resistant to therapy, hence the high rate of recurrence. Alterations of the tumour suppressor gene PTEN are prevalent in primary GBM, resulting in the inhibition of the polarity protein Lgl1 due to aPKC hyperactivation. Dysregulation of this molecular axis is one of the mechanisms involved in GSC maintenance. After demonstrating that the PTEN/aPKC/Lgl axis is conserved in Drosophila, I deregulated it in different cells populations of the nervous system in order to individuate the cells at the root of neurogenic brain cancers. This analysis identified the type II neuroblasts (NBs) as the most sensitive to alterations of this molecular axis. Type II NBs are a sub-population of Drosophila stem cells displaying a lineage similar to that of the mammalian neural stem cells. Following aPKC activation in these stem cells, I obtained an adult brain cancer model in Drosophila that summarises many phenotypic traits of human brain tumours. Fly tumours are indeed characterised by accumulation of highly proliferative immature cells and keep growing in the adult leading the affected animals to premature death. With the aim to understand the role of cell polarity disruption in this tumorigenic process I carried out a molecular characterisation and transcriptome analysis of brain cancers from our fly model. In summary, the model I built and partially characterised in this thesis work may help deepen our knowledge on human brain cancers by investigating many different aspects of this complicate disease.