Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates

The microplate nitrate reductase assay (MNRA) and the rezasurin microtitre assay (REMA) were used for the susceptibility testing of 73 clinical isolates and the results were compared with those that were obtained using the Bactec 460 TB and Bactec MGIT 960 systems. The REMA and the MNRA were performed in 96-well plates. For the REMA, the concentrations of isoniazid (INH) and rifampicin (RIF) ranged from 1.0-0.01 µg/mL and 2.0-0.03 µg/mL, respectively. For the MNRA, the INH concentration was between 1.0-0.03 µg/mL and the RIF concentration was between 2.0-0.06 µg/mL. For the MNRA, the sensitivity, specificity, positive predictive value, negative predictive value and INH/RIF agreement were 100/95.6, 97.6/100, 96.8/100, 100/98 and 98.6/98.6, respectively, and for the REMA, they were 100/91.3, 90.4/100, 88.5/100, 100/96.1 and 94.5/97.2, respectively. Our data suggest that these two rapid, low-cost methods may be inexpensive, alternative assays for the rapid detection of multidrug resistant tuberculosis in low-income countries.

4-7-19, stade Pershing, [Lawrence] Shields, Américain, à l'arrivée de la course de relais de 3000 m [des Jeux interalliés, le n°835 William C. Gray, membre du relais américain, lève le poing en signe de victoire] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54716

5-7-19, réception du maréchal Pétain à l'Académie des sciences : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54728

5-7-19, réception du maréchal Pétain à l'Académie des sciences : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54723

5-7-19, réception du maréchal Pétain à l'Académie des sciences : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 54722

Parc des Princes, 27/7/19, tour d'honneur de [Firmin] Lambot vainqueur du Tour de France : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55182

Parc des Princes, 27/7/19, [Firmin] Lambot vainqueur du Tour de France : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55180

Parc des Princes, 27/7/19, arrivée du Tour de France, en tête [Jean] Alavoine, [Luigi Natale] Lucotti, [Honoré] Barthélémy : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55179

Parc des Princes, 27/7/19, [Jean] Alavoine 2e du classement du Tour de France : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55185

Mme Susset [du club Les Mouettes de Paris, 27/7/19, championnat de Paris, natation, Choisy-le-Roi] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55191

Mme Decorne [du club Les Mouettes de Paris, 27/7/19, championnat de Paris, natation, Choisy-le-Roi] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55190

27/7/19 [illisible, championnat de Paris, natation, Choisy-le-Roi] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55189

The hypogene iron oxide copper-gold mineralization in the Mantoverde District, Northern Chile

The Mantoverde iron oxide copper-gold (IOCC) district, northern Chile, is known for its Cu production from supergene ores. Recently, exploration outlined an additional hypogene ore resource of 440 Mt with 0.56 percent Cu, and 0.12 g/t An. The hypogene sulfide mineralization occurs mainly as chalcopyrite and pyrite, typically in specularite or magnetite-cemented breccias and associated stockworks. The host rocks underwent variably intense K feldspar alteration, chloritization, sericitization, silicification, and/or carbonatization. A district scale Na(-Ca) alteration is absent. The IOCC mineralization in the district shows a strong tectonic control by northwest- to north-northwest-trending brittle structures. Large Cu sulfide-rich veins or Cu sulfide-cemented breccias are absent. Therefore, head grades of 4 percent Cu are an exception. There is a positive correlation between Cu and An grades. Gold is probably contained mostly in chalcopyrite and pyrite. Elevated concentrations of light rare-earth elements (LREE) occur locally but are attributed to redistribution of LREE within the deposits rather than to derivation from external sources. The Cu-Au ores in the Mantoverde district are low in and have relatively low contents in heavy metals that are potentially hazardous to the environment, such as As (avg 14 ppm), Hg (<5 ppm), or Cd (<0.2 ppm). The sulfur isotope ratios of chalcopyrite from the IOCC deposits lie between -5.6 and 8.9 per mil delta(34)S(VCDT). They show systematic variations within the district, which are interpreted to reflect relative distance to inferred fluid conduits and the level of deposition within the hydrothermal system. Most initial (87)Sr/(86)Sr values of altered volcanic rocks and hydrothermal calcite from the Mantoverde district are between 0.7031 and 0.7060 and are similar to those of the igneous rocks of the region. Lead isotope ratios of chalcopyrite are consistent with Pb (and by inference Cu) derived from Early Cretaceous magmatism. The sulfur, strontium, and lead isotope data of chalcopyrite, calcite gangue, or altered host rocks, respectively, are compatible with a genetic model that involves cooling of metal and sulfur-bearing magmatic-hydrothermal fluids that mix with meteoric waters or seawater at relatively shallow crustal levels. An additional exotic sulfur input is likely, though not required, for the copper mineralization. Apart from the IOCC. deposits, there are a number of smaller magnetite(-apatite) bodies in the district. These are geologically similar to the Cu-Au-bearing magnetite bodies, but are related to splays of the north-south-trending Atacama fault zone and differ in alteration and texture.

Parc des Princes, 27/7/19, [Jean] Alavoine 2e du classement du Tour de France, gagnant [de la] dernière étape : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 55186