Uptake of oxLDL and IL-10 production by macrophages requires PAFR and CD36 recruitment into the same lipid rafts

Macrophage interaction with oxidized low-density lipoprotein (oxLDL) leads to its differentiation into foam cells and cytokine production, contributing to atherosclerosis development. In a previous study, we showed that CD36 and the receptor for platelet-activating factor (PAFR) are required for oxLDL to activate gene transcription for cytokines and CD36. Here, we investigated the localization and physical interaction of CD36 and PAFR in macrophages stimulated with oxLDL. We found that blocking CD36 or PAFR decreases oxLDL uptake and IL-10 production. OxLDL induces IL-10 mRNA expression only in HEK293T expressing both receptors (PAFR and CD36). OxLDL does not induce IL-12 production. The lipid rafts disruption by treatment with βCD reduces the oxLDL uptake and IL-10 production. OxLDL induces co-immunoprecipitation of PAFR and CD36 with the constitutive raft protein flotillin-1, and colocalization with the lipid raft-marker GM1-ganglioside. Finally, we found colocalization of PAFR and CD36 in macrophages from human atherosclerotic plaques. Our results show that oxLDL induces the recruitment of PAFR and CD36 into the same lipid rafts, which is important for oxLDL uptake and IL-10 production. This study provided new insights into how oxLDL interact with macrophages and contributing to atherosclerosis development.

El bosque termófilo en Gran Canaria

Programa de Doctorado: Biodiversidad y conservación vegetal

Entornos robustos de programación distribuida

Programa de doctorado: Ingeniería de Telecomunicación Avanzada

Gestión costera en relación con los pequeños asentamientos litorales no ordenados

Máster Oficial en Gestión Costera

El litoral como área de gestión integrada. Costas Norte y Este de Gran Canaria

Análisis de la gestión integrada aplicable a las costas Norte y Este de Gran Canaria

Solemne acto de investidura de doctores : 26 enero 2008

Apertura de acto Dr. D, José Regidor García; Lectura de la memoria de investigación del curso 2006-2007; D. José Joaquín Hernández Brito; Conferencia Dr. D. Francisco José Marcellán Español; Reconocimiento al Dr. Antonio J. Fernández Rodríguez por su trayectoria universitaria.