Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina

High-voltage-activated calcium channels are hetero-oligomeric protein complexes that mediate multiple cellular processes, including the influx of extracellular Ca2+, neurotransmitter release, gene transcription, and synaptic plasticity. These channels consist of a primary α1 pore-forming subunit, which is associated with an extracellular α2δ subunit and an intracellular β auxiliary subunit, which alter the gating properties and trafficking of the calcium channel. The cellular localization of the α2δ3 subunit in the mouse and rat retina is unknown. In this study using RT-PCR, a single band at ∼305 bp corresponding to the predicted size of the α2δ3 subunit fragment was found in mouse and rat retina and brain homogenates. Western blotting of rodent retina and brain homogenates showed a single 123-kDa band. Immunohistochemistry with an affinity-purified antibody to the α2δ3 subunit revealed immunoreactive cell bodies in the ganglion cell layer and inner nuclear layer and immunoreactive processes in the inner plexiform layer and the outer plexiform layer. α2δ3 immunoreactivity was localized to multiple cell types, including ganglion, amacrine, and bipolar cells and photoreceptors, but not horizontal cells. The expression of the α2δ3 calcium channel subunit to multiple cell types suggests that this subunit participates widely in Ca-channel-mediated signaling in the retina.

Modeling and analysis of a zipping elastomeric actuator

This thesis introduces and analyzes a dielectric elastomer actuator (DEA) working in zipping mode. Electrostatic zipping is a very familiar actuation principle used in silicon micro-electro-mechanical systems. With lower voltage supply, electrostatic zipping can provide great performance for forces and displacements of an elastic membrane. Applying this principle to dielectric elastomers, the softness of the material will provide better compliance compared to silicon materials. After the presentation of an analytical model, this thesis investigates how system geometry and elastomer pre-tensioning affect system response. Results highlight how a proper selection of system parameters makes it possible to improve system regulation and reduce operating voltage requirements. Potential applications of zipping DEAs are for microfluidic control and electro-forming.

Transistor current -- voltage relationships : a consideration of high voltage dc operation of junction transistors with special reference to avalanche multiplication and related phenomena /

"August 6, 1963"

Some investigations in the area of high voltage, high current D-C amplifiers /

Issued also as thesis (M.S.) University of Illinois.

High voltage thermionic converters. Prepared under Contract no. AF33(615)-1533

"Project no. 8173, Task no. 817305."

Investigation of current-voltage and flow characteristics of an electric discharge across the exit of the AFFDL 1-Mw arc air heater

"Sponsored by the Air Force Flight Dynamics Laboratory ... under Contract AF 33(615)-1835."

Safe operation of capacitance meters using high applied-bias voltage /

Includes bibliographical references (p. 49).

Critical voltage levels as the criterion for planning power systems: Poland.

Mode of access: Internet.

A versatile high-voltage bias supply for extended range MIS C(V) and G(V) measurements /

Includes bibliographical references (p. 61).

Operator's manual including basic issue items list : test set, generator, and voltage regulator, automotive (Allen Electric and Equipment Co. model 30-82) (4910-270-3780).

"April 1965."

Operator's manual : test set, generator, and voltage regulator, automotive, 6, 12, and 24 volt systems (Sun Electric Corp., model no. VAT-25) (4910-270-3780).

"August 1966."

Operator, organizational, direct support, and general support maintenance manual (including repair parts and special tools list and depot maintenance repair parts and special tools) : test set, generator and voltage regulator, automotive, 12-and 24-volt systems (Allen Electric and Equipment Company model no. 30-92) (4910-092-9136).

"15 October 1971."