985 resultados para 26-251
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Background: Natural polyploidy has played an important role during the speciation and evolution of vertebrates, including anurans, with more than 55 described cases. The species of the Phyllomedusa burmeisteri group are mostly characterized by having 26 chromosomes, but a karyotype with 52 chromosomes was described in P. tetraploidea. This species was found in sintopy with P. distincta in two localities of São Paulo State (Brazil), where triploid animals also occur, as consequence of natural hybridisation. We analyse the chromosomes of P. distincta, P. tetraploidea, and their triploid hybrids, to enlighten the origin of polyploidy and to obtain some evidence on diploidisation of tetraploid karyotype.Results: Phyllomedusa distincta was 2n = 2x = 26, whereas P. tetraploidea was 2n = 4x = 52, and the hybrid individuals was 2n = 3x = 39. In meiotic phases, bivalents were observed in the diploid males, whereas both bivalents and tetravalents were observed in the tetraploid males. Univalents, bivalents or trivalents; metaphase II cells carrying variable number of chromosomes; and spermatids were detected in the testis preparations of the triploid males, indicating that the triploids were not completely sterile. In natural and experimental conditions, the triploids cross with the parental species, producing abnormal egg clutches and tadpoles with malformations. The embryos and tadpoles exhibited intraindividual karyotype variability and all of the metaphases contained abnormal constitutions. Multiple NORs, detected by Ag-impregnation and FISH with an rDNA probe, were observed on chromosome 1 in the three karyotypic forms; and, additionally, on chromosome 9 in the diploids, mostly on chromosome 8 in the tetraploids, and on both chromosome 8 and 9 in the triploids. Nevertheless, NOR-bearing chromosome 9 was detected in the tetraploids, and chromosome 9 carried active or inactive NORs in the triploids. C-banding, base-specific fluorochrome stainings with CMA3 and DAPI, FISH with a telomeric probe, and BrdU incorporation in DNA showed nearly equivalent patterns in the karyotypes of P. distincta, P. tetraploidea, and the triploid hybrids.Conclusions: All the used cytogenetic techniques have provided strong evidence that the process of diploidisation, an essential step for stabilising the selective advantages produced by polyploidisation, is under way in distinct quartets of the tetraploid karyotype. © 2013 Gruber et al.; licensee BioMed Central Ltd.
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The aim of this work was to determine crop coefficients (Kc) of drip irrigated watermelon in the climatic and growing conditions of the Gurgueia Vale, State of Piaui, Brazil, located at 8 ° 26' S, 43 ° 47' W and altitude of 251 m. The reference evapotranspiration (ET0) was determined by the sum of hourly values of ET0 obtained by the Penman-Monteith method parameterized by FAO with climatic data obtained from an automatic weather station. The daily crop evapotranspiration (ETc) was measured by three weighing lysimeters of load cells. Aiming high fruit yield in this region, we recommend that the following local values of Kc and Kcb be used for planning and management of irrigation, respectively: initial stage (crop establishment) - 0.34 and 0.24; intermediate stage (growth and fruit maturation) - 1.16 and 1.10; end stage (harvest) - 0.93 and 0.86. These Kc values of initial and intermediate phases are statistically higher than the values of Kc and Kcb already adjusted according to the methodology presented in the FAO Irrigation and Drainage Paper 56. The values of Kc and Kcb at the end phase are not statistically different from the FAO values.
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Este documento es parte del informe anual de la CEPAL que cubre el período comprendido entre el 15 de febrero de 1964 y el 17 de mayo de 1965. Fue publicado como documento oficial del Consejo Economico y Social de las Naciones Unidas, 39o. período de sesiones, Suplemento No. 4 (E/4032/Rev.l y E/CN.12/731/Rev.2).