Iowa AgriNews, December 29, 2015, Vol. 15, no. 25

Crop and livestock summaries for the state of Iowa, produced by the Iowa Department of Agriculture. Previously Agri-News

Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR

O objetivo deste trabalho foi avaliar a expressão, por RT-qPCR, dos genes de nodulação nodC e nodW e do gene nopP da estirpe CPAC 15, que provavelmente atuam na infecção das raízes da soja. Foram realizados dois experimentos. No primeiro, a expressão dos genes foi avaliada nas células após a incubação com genisteína por 15 min, 1, 4 e 8 horas. Os resultados revelaram que os três genes apresentaram maior expressão imediatamente após o contato com o indutor (15 min). No segundo experimento, a bactéria foi cultivada na presença de indutores (genisteína ou exsudatos de sementes de soja) por 48 horas. A expressão dos três genes foi maior na presença de genisteína, com valores de expressão para nodC, nodW e nopP superiores ao controle. Os resultados obtidos confirmam a funcionalidade dos três genes na estirpe CPAC 15, com ênfase para o nopP, cuja funcionalidade em Bradyrhizobium japonicum foi descrita pela primeira vez.

FY15 Enrich Iowa Report State Funding for Iowa Libraries, January 15, 2015

This report illustrates the critical role of Enrich Iowa funding in enhancing lifelong learning for Iowans through libraries; improving library resources aimed at assisting job seekers; maintaining library hours that meet library customers’ needs; improving library technology services; and providing safe, accessible library buildings.

15/2/20, M. Poincaré à Thionville [avec à sa droite la grande-duchesse de Luxembourg, cérémonie de remise de la croix d'honneur à la ville] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58019

15/2/20, Thionville, la grande-duchesse Charlotte [de Luxembourg] et M. Poincaré [revue des troupes, le jour de la remise de la croix d'honneur à la ville] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58022

15/2/20, Thionville [M. Poincaré avec à sa droite la grande-duchesse de Luxembourg, cérémonie de remise de la croix d'honneur à la ville] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58024

15/2/20, M. Poincaré à Thionville [avec à sa droite la grande-duchesse de Luxembourg, estrade située place du marché, cérémonie de remise de la croix d'honneur à la ville] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58025

15/2/20, M. Poincaré à Thionville [avec à sa droite la grande-duchesse de Luxembourg, place du marché, défilé de jeunes lorraines en costume, cérémonie de remise de la croix d'honneur à la ville] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58027

FY'15 Biodiesel Fuel Revolving Fund Expenditures Report

Pursuant to Iowa Code section 307.20, the biodiesel fuel revolving fund (Fund) was created and is to be used to purchase biodiesel fuel for use in the Department of Transportation’s vehicles. The act directed that the Fund receive money from the sale of EPAct credits banked by the DOT on the effective date of the act, monies appropriated by the General Assembly, and any other monies obtained or accepted by the DOT for deposit in the Fund. This report is of the expenditures made from the Fund during FY 2015.

An overview of L-2-hydroxyglutarate dehydrogenase gene (L2HGDH) variants: a genotype-phenotype study.

L-2-Hydroxyglutaric aciduria (L2HGA) is a rare, neurometabolic disorder with an autosomal recessive mode of inheritance. Affected individuals only have neurological manifestations, including psychomotor retardation, cerebellar ataxia, and more variably macrocephaly, or epilepsy. The diagnosis of L2HGA can be made based on magnetic resonance imaging (MRI), biochemical analysis, and mutational analysis of L2HGDH. About 200 patients with elevated concentrations of 2-hydroxyglutarate (2HG) in the urine were referred for chiral determination of 2HG and L2HGDH mutational analysis. All patients with increased L2HG (n=106; 83 families) were included. Clinical information on 61 patients was obtained via questionnaires. In 82 families the mutations were detected by direct sequence analysis and/or multiplex ligation dependent probe amplification (MLPA), including one case where MLPA was essential to detect the second allele. In another case RT-PCR followed by deep intronic sequencing was needed to detect the mutation. Thirty-five novel mutations as well as 35 reported mutations and 14 nondisease-related variants are reviewed and included in a novel Leiden Open source Variation Database (LOVD) for L2HGDH variants (http://www.LOVD.nl/L2HGDH). Every user can access the database and submit variants/patients. Furthermore, we report on the phenotype, including neurological manifestations and urinary levels of L2HG, and we evaluate the phenotype-genotype relationship.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, March 15, 2016; With Addendums

Weekly letting report

Monday Morning Eye Opener, February 15, 2016

Weekly Newsletter from the Northwest District Office for libraries containing programs, activities, classes for the upcoming week.

EPI Update, January 15, 2016

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Quick Reads, December 15, 2015

IDPH Quick Reads is an electronic newsletter produced by the Director’s Office at the Iowa Department of Public Health. IDPH Quick Reads are published every three to four weeks.