Phylogenetic relationships among two species of golden monkey and three species of leaf monkey inferred from rDNA variation

Restriction maps of rDNA repeats of five species of Colobinae and three outgroup taxa, Hylobates leucogenys, Macaca mulatta, and Macaca irus, were constructed using 15 restriction endonucleases and cloned 18S and 28S rRNA gene probes. The site variation between Rhinopithecus roxellana and Rhinopithecus bieti is comparable to that between Presbytis francoisi and Presbytis phayrei, implying that R. bieti is a valid species rather than a subspecies of R. roxellana. Phylogenetic analysis on the 47 informative sites supports the case for Rhinopithecus being an independent genus and closely related to Presbytis. Furthermore, branch lengths of the tree seem to support the hypothesis that the leaf monkeys share some ancestral traits as well as some automorphic characters.

Heliconema savala n.sp. (Nematoda:Physalopteridae) from marine fish Lepturacanthus savala (Cuvier) (Trichiuridae) and Psettodes erumei (Schneider) new host (Pisces:Pettodidae) of Physalopterid Nematode, Bulbocephalus inglisi Rasheed, 1966 in Pakistan

Helicnonema savala, n.sp. obtained from the marine fish, Lepturacanthus savala in Sindh coast is distinguished from members of the genus processing in the male 10 tessellated longtitudinal ridges and a spicule ratio 1:15. Females have vulvular flap. Heliconema savala is a morphologically most closely related to Heliconema heliconema. The marine fish, Psettodes erumei is recorded as a new host of Bulbocephalus inglisi.

The behavior of a single pile under cyclic axial loads

Many piled foundations have been destroyed under significant cyclic loads in earthquakes. Centrifuge modelling of a single pile subjected to cyclic loads has been conducted to investigate the influence of cyclic loads on the axial performance of the single pile. Different pile installation procedures were applied to compare the axial behaviour of different piles under cyclic loads. Pile head permanent settlements accumulated due to cyclic axial loads, and these increased with the increasing load amplitude. Also the pile head axial secant stiffness decreased with the increasing number of axial load cycles, and with increasing amplitude. Furthermore, the axial pile performance is influenced significantly by different installation methods. © 2010 ASCE.

Effects of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation and characterization of a prothrombin activator

The action of Pallas' viper (Agkistrodon halys pallas) venom on blood coagulation was examined in vitro and a strong anticoagulant effect was observed. This action was abolished after treatment with a specific inhibitor of phospholipase A(2) activity (p-bromophenacyl bromide), revealing a procoagulant action in low concentrations of treated venom (around 1 mu g/ml). The effect of the venom an haemostasis was further characterized by measuring its ability to activate purified blood coagulation factors. It is concluded that A. halys pallas venom contains prothrombin activation activity. A prothrombin activator (aharin) was purified from the venom by Sephadex G-75 gel filtration and ion-exchange chromatography on a Mono-Q column. It consisted of a single polypeptide chain, with a mol. wt of 63,000. Purified aharin possessed no amidolytic activity on chromogenic substrates. It did not act on other blood coagulation factors, such as factor X and plasminogen, nor did it cleave or clot purified fibrinogen. The prothrombin activation activity of aharin was readily inhibited by ethylenediamine tetracetic acid (a metal chelator), but specific serine protease inhibitors such as diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride had no effect on it. These observations suggest that, like those prothrombin activators from Echis carinatus and Bothrops atrox venoms, the prothrombin activator from A. halys pallas venom is a metalloproteinase. (C) 1998 Elsevier Science Ltd. All rights reserved.

Fishery, population characteristics and stock assessment of cuttlefishes, Sepia aculeata and Sepia pharaonis at Kakinada along the east coast of India

Cuttlefishes were exploited exclusively by trawls along the coast. Growth, recruitment, mortality and exploitation rates of Sepia aculeata and Sepia pharaonis were studied. Fishery of the former was supported mainly by zero year and the latter by zero and one + age groups. Both attain sexual maturity during the first year itself and spawn during August-March with peak during November-December. Natural mortality of S.aculeata was 2.22, fishing mortality 2.34 and total mortality 4.56. It was 1.69, 1.97 and 3.66 respectively for S.pharaonis. Exploitation rate was 0.52 and Emax 0.72 for S.aculeata and it was 0.54 and 0.76 respectively for S.pharaonis. Their mortality and exploitation rates indicated that stock remains under-exploited and have considerable scope for improving the production. However, both stock and catch exhibited wide annual fluctuation with declining trend during the period. These necessitated immediate attention including measures to minimise juvenile exploitation for improving stock and fishery.

Apoptotic activity of frog Bombina maxima skin albumin

Albumin, the most abundant protein components of blood plasma, is synthesized and secreted by liver cells in vertebrates. Recently, it was demonstrated that frog Bombina maxima albumin is also expressed in skin. Both B. maxima albumins from skin and serum

休克时肾脏脂质过氧化损伤的实验研究

本实验用家兔20只,以夹闭肠系膜上动脉造成肠系膜上动脉夹闭性休克(SMAO)模型。经测定红细胞超氧化物歧化酶(SOD)活性,血浆及肾组织中自由基脂质过氧化的代谢产物丙二醛(MDA)含量的变化。发现SMAO时,血浆及肾组织MDA含量明显增高,而红细胞SOD活性降低。提示:氧自由基及其介导的脂质过氧化对其病变过程起了一定的作用。

Distribution, biology and behaviour of the giant trevally, Caranx ignobilis- a candidate species for mariculture

Fishery and biology of the giant trevally, Caranx ignobilis exploited along the Tuticorin coast of Tamilnadu were monitored during 2001-2006. Fishery occurred round the year with peak landings during April-August. Spawning and recruitment occur almost round the year with peak during November-December. Young ones are abundant in shallow coastal waters and as grows, they move to deeper waters. Growth parameters, L"' and K are estimated respectively as 143.6 cm and 0.69/year and 'to' as -0.0242 year. Estimates show that they grow fast and attain 73, 108, 126 and 134 cm in total length by first, second, third and fourth year respectively. Their weight increment is also fast and attains 5.5 kg, 16.8 kg, 25.9 kg and 33.7 kg respectively during the period. Stock assessment indicated that the stock at present is over exploited and under heavy fishing pressure. Rearing trial in aquarium tank showed that they are compatible to confined rearing conditions. Based on the distribution and biology of the species, their mariculture potential is discussed.

Simulating intertwined design processes that have similar structures: A case study of a small company that creates made-to-order fashion products

The authors use simulation to analyse the resource-driven dependencies between concurrent processes used to create customised products in a company. Such processes are uncertain and unique according to the design changes required. However, they have similar structures. For simulation, a level of abstraction is chosen such that all possible processes are represented by the same activity network. Differences between processes are determined by the customisations that they implement. The approach is illustrated through application to a small business that creates customised fashion products. We suggest that similar techniques could be applied to study intertwined design processes in more complex domains. Copyright © 2011 Inderscience Enterprises Ltd.

Degradation of tropical forest in Hainan, China, 1991-2008: Conservation implications for Hainan Gibbon (Nomascus hainanus)

The Hainan gibbon (Nomascus hainanus) is one of the most endangered primates in the world, confined to mature natural forest in Hainan Island, China. We assessed changes in habitat condition on the island between 1991 and 2008, using vegetation maps generated by remote-sensing images. We defined forest suitable for gibbons based on composition, tree size and canopy cover. During the 17-year period, the area of suitable gibbon forest decreased by 540 km(2) (35%) across the whole island, and by 6.3 km(2) (7%) in the locality of the sole remaining gibbon population at Bawangling National Nature Reserve. The forest patches large enough (>1 km(2)) to support a gibbon group decreased from 754 km(2) to 316 km(2) in total area, and from 92 to 64 in number. Suitable natural forest was mainly replaced by plantations below 760 m, or degraded by logging, grazing and planting of pines above 760 m. Meanwhile, forests in former confirmed gibbon areas became more fragmented: mean area of patches decreased by 53%. We mapped the patches of natural forest in good condition which could potentially support gibbons. We recommend a freeze on further expansion of plantations between core patches at Bawangling, Jiaxi-Houmiling and Yinggeling Nature Reserves in accordance with forest protection regulations; establishment of nature reserves in currently unprotected natural forest patches elsewhere in line with the local government's nature reserve expansion policy; and active natural-forest restoration between remaining fragments at Bawangling. (C) 2010 Elsevier Ltd. All rights reserved.

Developmental Expression of an Amphioxus (Branchiostoma belcheri) Gene Encoding a GATA Transcription Factor

GATA基因在脊椎动物和非脊椎动物的发育中行使重要的功能,该家族的成员在进化上也足非常保守的.脊椎动物的GATA基因分为两个亚群:GATA1/2/3和GATA4/5/6.通过生物信息分析,在文吕鱼的基因缓中找到了3个GATA基因:一个GATA1/2/3业家族基因,两个GATA4/5/6亚家族基因:还找到一个类GATA基因.还克隆了白氏文昌鱼(Branchiostoma belcheri)GATA123的一段序列,并研究了它在早期胚胎发育中的表达图式.结果表明GATA123在原肠胚的中内胚层表达,而在神经胚晚期和幼体早期,GATA123在脑泡和消化道中部区域表达.这种表达模式与头部发育的重要基因Otx相类似.结果提示在文吕鱼脑泡的发育过程中GATA123和Otx很可能共同发挥着重要的作用.

Studies on threshold current densities for convulsion of fish using a square wave stimulator

The threshold current densities and voltage tensions (body voltages) between the head and tail for bringing about distinct reactions in Salmo irideus, Cyprinus Carpio, Tinea tinca, Gasterosteus aculeatus and Salmo fario were studied. In C. carpio and T. tinca, absolute current densities required decreased with increase in length of fish. Threshold current densities for different reactions of fish increased with rise in water temperature and conductivity of surrounding medium except in case of T. tinca where low current densities were sufficient in higher conductivity of water. Impulse D.C. was superior to continuous D.C. Better effect was noticed in fishes in lower current densities when their bodies were parallel to the lines of current conduction.

猪线粒体DNA多态性与中国地方猪种起源分化的关系

用24种限制性内切酶分析了我国21个具代表性地方猪品种、1个引进品种和2个来自中国和越南的野生近缘种mtDNA的RFLP。结果表明:在74个个体中检出的32种限制性态型可归结成7种单倍型,其间的差异主要来源于少数几个限制性位点的点突变;地方猪种4种单倍型间的平均遗传距离为0.143％,遗传多态程度(π值)仅为0.007％,说明遗传多样性非常贫乏,提示中国地方猪种可能起源于一个野猪亚种。

On the vertical distribution of seers and other commercially important fishes in the surface drift nets

This note highlights the author's attempts to determine the vertical height for catching seers (Scomberomorus sp.) and other commercially important fish off Kakinada coast.