Climatic changes in Eurasia and Africa at the last glacial maximum and mid-Holocene: reconstruction from pollen data using inverse vegetation modelling

IEECAS SKLLQG

Evolution of vegetation and climate since the last glacial maximum recorded at Dahu peat site；South China

IEECAS SKLLQG

Response of vegetation in the Qinghai-Tibet Plateau to global warming

IEECAS SKLLQG

Vegetation and climate variations at Taibai, Qinling Mountains in central China for the last 3 500 cal BP

IEECAS SKLLQG

Carbon isotopic composition of modern soil and paleosol as a response to vegetation change on the Chinese Loess Plateau

IEECAS SKLLQG

The vegetation and climate change during Neocene and Early Quaternary in Jiuxi Basin, China

IEECAS SKLLQG

Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry

Peptide mass mapping analysis, utilizing a regenerable enzyme microreactor with metal-ion chelated adsorption of enzyme, combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was developed. Different procedures from the conventional approaches were adopted to immobilize the chelator onto the silica supports, that is, the metal chelating agent of iminodiacetic acid (IDA) was reacted with glycidoxypropyltrimethoxysilane (GLYMO) before its immobilization onto the inner wall of the fused-silica capillary pretreated with NH4HF2. The metal ion of copper and subsequently enzyme was specifically adsorbed onto the surface to form the immobilized enzyme capillary microreactor, which was combined with MALDI-TOF-MS to apply for the mass mapping analysis of nL amounts of protein samples. The results revealed that the peptide mapping could routinely be generated from 0.5 pmol protein sample in 15 min at 50degreesC, even 20 fmol cytochrome c could be well digested and detected.