Análise microbiológica e físico-química de material proteico comestível derivado do processamento da gordura oriunda do abate de bovinos

Background: The use of all by-products of bovine slaughter is of high economic importance for the industries of products of animal origin. Among these products, fat has an important role, once fat rendering may generate several different products, such as protein material that may be used in the manufacture of meat products. However, in spite of the importance that the use of all by-products has for the economic balance of the industry, there are no reports on their use in Brazil, or studies that supply data on microbiological and physical-chemical local standards for this protein. Thus, the objective of this study was to evaluate microbiological and physical-chemical characteristics of protein material obtained from fat rendering, as well as to provide support for companies to use fat rendering to generate protein material, adding value to industrialized meat products.Materials, Methods & Results: The experimental production of edible protein obtained of fat rendering was conducted in slaughterhouse with supervision of the Brazilian Ministry of Agriculture, Livestock and Food Supply. Protein material was obtained in a continuous, humid heat system at high temperatures. Fat scraps containing protein were ground and cooked at high temperature (85 degrees C), and placed in a three phase decanter centrifuge. After centrifugation, protein material was ground again and packed. Samples were collected from 15 batches of protein material, and the following microbiological analyses were carried out: counts of aerobic mesophilic and psychrotrophic microorganisms, coliforms at 35 degrees C, Escherichia coli, sulfite-reducing Clostridium, and Staphylococcus aureus, besides presence or absence of Salmonella and Listeria monocytogens. The following physical-chemical analyses were also carried out: protein, total lipid, moisture, ash, carbohydrate, and energy content. Mean counts of mesophiles, psychrotrophs, and coliforms at 35 degrees C were 4.17; 3.69 and 1.87 (log CFU/g), respectively. Levels of protein, total lipids, moisture, ashes and carbohydrates were 27.50; 7.83; 63.88%; 0.24%; and 0.55%, respectively, and energy content was 182.63 kcal/100g.Discussion: Results of microbiological analyses demonstrated that, although low, the final product showed to be contaminated. Contamination that occurred during the second grinding procedure may be an explanation for these bacterial counts. Also, the temperature used for fat fusion was not enough to eliminate thermoduric microorganisms. However, even with the presence of indicator microorganisms in the samples, none was contaminated by E. coli, sulfite-reducing Clostridium, S. aureus, Salmonella or L. monocytogenes. Physical-chemical analyses showed that the product had adequate nutritional quality. Based on these results, it was possible to conclude that protein material obtained in fat rendering showed characteristics that enable the use of this product as raw material for processed meat products. Besides, the present study was the first one to present scientific results in relation to edible by-products obtained in fat rendering, supplying important information for slaughterhouses and meat-processing plants. The study also produced relevant data on the innocuousness of the product, which may be used to guide decision-making of health inspectors.

Validation of analytical methodology for quantification of cefazolin sodium pharmaceutical dosage form by high performance liquid chromatography to be applied for quality control in pharmaceutical industry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cidadania e práticas escolares: as representações de um grupo de alunos do ensino médio de uma escola pública paulista

Pós-graduação em Educação Escolar - FCLAR

Desenvolvimento de experiências de acionamento e controle do movimento angular de motores de indução trifásico

This paper aims to show practical and effectiveexperiencesfor lessons Industrial Automation Laboratory taught inundergraduate degreein ElectricalEngineering from the University Júlio MesquitaFilho - UNESP, Guaratinguetá. Experiments carriedsimulatecontrol and drive systems of electric three phase induction motors (MIT)widely usedinindustries. The experiments simulate a manufacturing environment where there isa need to control the activation and continuous operation ofelectricmotors. Seven experimentsthat simulatethe firing of electrical motors through a controlsystem, a driver along with asimulator loads coupled to the electric motor was developed. Experiments usinga Programmable Logic Controller (PLC) as acontroller,an inverter frequencyasdriver, and MagneticBrake, as simulatorengine loads . The experiments were divided accordingto the speed reference signal used fordrivingand operating the electric motor: digital and analog. The first five experiments performing the drive control and operation of the electric motor via digital signals. The sixth and seventh experiments using an analog signal as a reference speed for the electric motor

Controle de velocidade de um motor de indução trifásico usando técnicas de ajuste empírico para bancada de teste no laboratório de automação

This work aims to make the closed loop control of a three phase induction motor, through the integration of the following equipment: a frequency inverter, the actuator system; a programmable logic controller (PLC), the controller; an encoder, the velocity sensor, used as a feedback monitoring the control variable and the three-phase induction motor, the plant to be controlled. The control is performed using a Proportional - Integrative - Derivative (PID) approach. The PLC has a help instruction, which performs the auto adjustment of the controller, that instruction is used and confronted with other adjustment methods. There are several types of methods adjustments to the PID controllers, where the empirical methods are addressed in this work. The system is deployed at the Interface and Electro Electronic Control laboratory in the Universidade Estadual Paulista Júlio Mesquita Filho, Guaratinguetá, São Paulo, then, in the future, this work becomes an experiment to be conducted in the classroom, allowing undergraduate students to develop a greater affinity to the programs used by the PLC as well as studies of undergraduate and graduate works with the help of assembly made

Proposta de uma metodologia de rebobinamento para converter motor de indução trifásico em monofásico

This paper proposes the conversion of a three-phase induction motor into single phase through rewinding its stator. It presents a methodology to calculate this rewinding process considering the constructive parameters of the, such as number of slots and number of poles, for instance. The operating principles of DC motors are highlighted to introduce the logic involved in the calculation of lap and wave windings. The construction of the stator three-phase induction motor generally uses the lap winding, which is also used in single phase to perform the conversion. The rewinding process is widely used for reconstruction of industrial and residential electric motors that have suffered damage or present flaws with use. Therefore, many three-phase electric motors that are discarded or disposed of could be recovered by the method described in this work for a purpose that contributes to energy efficiency or simply saving resources in single-phase uses

Rememorando vidas: lembranças de velhos trabalhadores

Pós-graduação em Serviço Social - FCHS

Uso de redes neurais com adaptação de pesos por modos deslizantes para controle de sistemas e aplicações em máquinas elétricas

Pós-graduação em Engenharia Elétrica - FEIS

Modelo matemático para o controle ótimo de Volt/VAr em sistemas de distribuição de energia elétrica trifásicos

Pós-graduação em Engenharia Elétrica - FEIS

Color stability of a nanofilled resin: influence of polishing and finishing and fluoride solutions according to time

Aim: The aim of the study was evaluate the finishing and polishing effect of the color stability of the composite resin Filtek Supreme XT, according to different fluoride solutions and time. Material and Methods: Specimens were prepared (n=140) with half of the samples finished and polished. The experimental groups were divided according to the presence or absence of finishing and polishing and immersion solutions (artificial saliva, sodium fluoride solution at 0.05% - manipulated, Fluordent Reach, Oral B, Fluorgard). The specimens remained in artificial saliva for 24 hours and were subjected to an initial color analysis using a spectrophotometer CIELab system. Then, they were immersed in the experimental solutions for 1 minute a day. The readings of the color change were made after 24 and 48 hours, 7, 14, 21, 30 and 60 days after the first immersion. The three-way mixed Analysis of Variance (ANOVA) ("finishing/polishing", "immersion medium" and “time”) were performed. For multiple comparisons, the Sidak test for repeated measure was used, with a 5% level of significance. Results: The finishing and polishing factor showed significant variability, independently of the immersion media (p<0.001). Cconclusion: Finishing and polishing procedures yielded better color stability to composite resin over time, regardless of the immersion media.

Estimação de parâmetros de linhas de transmissão utilizando técnicas de decomposição modal: Aplicação em linhas com plano de simetria vertical

Pós-graduação em Engenharia Elétrica - FEIS

Moisture relationships for the Southern Hemisphere

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reliability and differentiation capability of dynamic and static kinematic measurements of rearfoot eversion in patellofemoral pain

Excessive rearfoot eversion is thought to be a risk factor for patellofemoral pain development, due to the kinesiological relationship with ascendant adaptations. Individuals with patellofemoral pain are often diagnosed through static clinical tests, in scientific studies and clinical practice. However, the adaptations seem to appear in dynamic conditions. Performing static vs. dynamic evaluations of widely used measures would add to the knowledge in this area. Thus, the aim of this study was to determine the reliability and differentiation capability of three rearfoot eversion measures: rearfoot range of motion, static clinical test and static measurement using a three-dimensional system. A total of 29 individuals with patellofemoral pain and 25 control individuals (18-30 years) participated in this study. Each subject underwent three-dimensional motion analysis during stair climbing and static clinical tests. Intraclass correlation coefficient and standard error measurements were performed to verify the reliability of the variables and receiver operating characteristic curves to show the diagnostic accuracy of each variable. In addition, analyses of variance were performed to identify differences between groups. Rearfoot range of motion demonstrated higher diagnostic accuracy (an area under the curve score of 0.72) than static measures and was able to differentiate the groups. Only the static clinical test presented poor and moderate reliability. Other variables presented high to very high values. Rearfoot range of motion was the variable that presented the best results in terms of reliability and differentiation capability. Static variables do not seem to be related to patellofemoral pain and have low accuracy values.

Barriers related to non-adherence in a mammography breast-screening program during the implementation period in the interior of São Paulo State, Brazil

Mammography is the best exam for early diagnosis of breast cancer. Developing countries frequently have a low income of mammography and absence of organized screening. The knowledge of vulnerable population and strategies to increase adherence are important to improve the implementation of an organized breast-screening program. A mammography regional-screening program was implemented in a place around 54.238 women, aged 40-69 years old. It was proposed to perform biannual mammography free of cost for the women. We analyze the first 2 years of the implementation of the project. Mammography was realized in 17.964 women. 42.1% of the women hadn't done de mammography in their lives and these women were principally from low socio-economic status (OR=2.99), low education (OR=3.00). The best strategies to include these women were mobile unit (OR=1.43) and Family Health Program (OR=1.79). The incidence of early breast tumors before the project was 14.5%, a fact that changed to 43.2% in this phase. Multivariate analysis showed that the association of illiterate and the mobile unit achieve more women who had not performed mammography in their lives. The strategies to increase adherence to mammography must be multiple and a large organization is necessary to overpass the barriers related to system health and education.

Validation of analytical methodology for quantification of cefazolin sodium by liquid chromatography

A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2 =0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies. The method demonstrated satisfactory results for precision, accuracy, limits of detection and quantitation. The robustness of this method was evaluated using the Plackett–Burman fractional factorial experimental design with a matrix of 15 experiments and the statistical treatment proposed by Youden and Steiner. Finally, the proposed method could be also an advantageous option for the analysis of cefazolin sodium, contributing to improve the quality control and to assure the therapeutic efficacy