QuEChERS: a new sample preparation approach for the determination of ibuprofen and its metabolites in soils

Ibuprofen is one of the most used active pharmaceutical ingredients worldwide. A new method for the analysis of ibuprofen and its metabolites, hydroxyibuprofen and carboxyibuprofen, in soils is presented. The extraction of these compounds from the soil matrices was performed by using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. The method involves a single extraction of the investigated compounds with purified water (acidified at pH 2.5 with hydrochloric acid), and a slow and continuous addition of the QuEChERS content, followed by the addition of acidified acetonitrile (1% acetic acid), prior to the determination by liquid chromatography coupled with fluorescence detection (LC–FLD). Validation studies were carried out using soil samples with a range of organic carbon contents. Recoveries of the fortified samples ranged from 79.5% to 101%. Relative standard deviations for all matrix–compound combinations did not exceed 3%. The method quantification limits were ≤22.4 μg kg−1 in all cases. The developed method was applied to the analysis of sixteen real samples.

Adaptive mutations in bacteria: high rate and small effects

Evolution by natural selection is driven by the continuous generation of adaptive mutations. We measured the genomic mutation rate that generates beneficial mutations and their effects on fitness in Escherichia coli under conditions in which the effect of competition between lineages carrying different beneficial mutations is minimized. We found a rate on the order of 10–5 per genome per generation, which is 1000 times as high as previous estimates, and a mean selective advantage of 1%. Such a high rate of adaptive evolution has implications for the evolution of antibiotic resistance and pathogenicity.

Lipid extractions from marine deep-sea invertebrates : modifications to the Bligh & Dyer method to increase lipid yields

The deep-sea environment is difficult to sample, and often only small quantities of samples can be obtained when using less destructive methods than dredging. When working with marine animals that are difficult to sample and with limited quantities of tissue to extract lipids, it is essential to ensure that the used method extracts the maximum possible quantity of lipids. This study evaluates the efficiency of introducing modifications to the method originally described by Bligh & Dyer (1959). This lipid extraction method is broadly used with modifications, although these usually lack proper description and evaluation of increment in lipids. In this study we consider the improvement in terms of amount of lipids extracted by changing the method. Lipid content was determined by gravimetric measurements in eight invertebrates from the deep-sea, including deep-sea hydrothermal vents animals, using three different approaches. Results show increases of 14% to 30% in lipid contents obtained from hydrothermal vent invertebrate tissues and whole animals by placing the samples in methanol for 24 hours before applying the Bligh & Dyer mixture. Efficiency of the extractions using frozen and freeze-dried samples was also compared. For large sponges, the use of lyophilized materials resulted in increases of 3 to 7 times more lipids extracted when compared with extractions using frozen samples.

Management of agricultural waste on a sample of farms on the island of São Miguel (Açores)

Dissertação de Mestrado em Ambiente, Saúde e Segurança.

Optimal strategies for marine wildlife tourism in small islands

Tese de Doutoramento, Ciências Económicas e Empresariais (especialidade de Economia), 18 de Junho de 2015, Universidade dos Açores

O efeito da idade da empresa nos salários

Dissertação de Mestrado, Ciências Económicas e Empresariais, 10 de Dezembro de 2015, Universidade dos Açores.

Determinação de propriedades mecânicas através de ensaios de provetes miniatura, "Small Punch"

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Mecânica

O contributo da implementação de um sistema de controlo interno robusto para as pequenas e médias empresas portuguesas: o caso das agências de meios e publicidade

Mestrado em Auditoria

Strabismus, visual acuity, and uncorrected refractive error in Portuguese children aged 6 to 11 years

Introduction: Visual anomalies that affect school-age children represent an important public health problem. Data on the prevalence are lacking in Portugal but is needed for planning vision services. This study was conducted to determine the prevalence of strabismus, decreased visual acuity, and uncorrected refractive error in Portuguese children aged 6 to 11 years. Methods and materials: A cross-sectional study was carried out on a sample of 672 school-age children (7.69 ± 1.19 years). Children received an orthoptic assessment (visual acuity, ocular alignment, and ocular movements) and non-cycloplegic autorefraction. Results: After orthoptic assessment, 13.8% of children were considered abnormal (n = 93). Manifest strabismus was found in 4% of the children. Rates of esotropia (2.1%) were slightly higher than exotropia (1.8%). Strabismus rates were not statistically significant different per sex (p = 0.681) and grade (p = 0.228). Decreased visual acuity at distance was present in 11.3% of children. Visual acuity ≤20/66 (0.5 logMAR) was found in 1.3% of the children. We also found that 10.3% of children had an uncorrected refractive error. Conclusions: Strabismus affects a small proportion of the Portuguese school-age children. Decreased visual acuity and uncorrected refractive error affected a significant proportion of school-age children. New policies need to be developed to address this public health problem.

Cholesterol 24S-Hydroxylase overexpression inhibits the liver X receptor (LXR) pathway by activating small guanosine triphosphate-binding proteins (sGTPases) in neuronal cells

The neuronal-specific cholesterol 24S-hydroxylase (CYP46A1) is important for brain cholesterol elimination. Cyp46a1 null mice exhibit severe deficiencies in learning and hippocampal long-term potentiation, suggested to be caused by a decrease in isoprenoid intermediates of the mevalonate pathway. Conversely, transgenic mice overexpressing CYP46A1 show an improved cognitive function. These results raised the question of whether CYP46A1 expression can modulate the activity of proteins that are crucial for neuronal function, namely of isoprenylated small guanosine triphosphate-binding proteins (sGTPases). Our results show that CYP46A1 overexpression in SH-SY5Y neuroblastoma cells and in primary cultures of rat cortical neurons leads to an increase in 3-hydroxy-3-methyl-glutaryl-CoA reductase activity and to an overall increase in membrane levels of RhoA, Rac1, Cdc42 and Rab8. This increase is accompanied by a specific increase in RhoA activation. Interestingly, treatment with lovastatin or a geranylgeranyltransferase-I inhibitor abolished the CYP46A1 effect. The CYP46A1-mediated increase in sGTPases membrane abundance was confirmed in vivo, in membrane fractions obtained from transgenic mice overexpressing this enzyme. Moreover, CYP46A1 overexpression leads to a decrease in the liver X receptor (LXR) transcriptional activity and in the mRNA levels of ATP-binding cassette transporter 1, sub-family A, member 1 and apolipoprotein E. This effect was abolished by inhibition of prenylation or by co-transfection of a RhoA dominant-negative mutant. Our results suggest a novel regulatory axis in neurons; under conditions of membrane cholesterol reduction by increased CYP46A1 expression, neurons increase isoprenoid synthesis and sGTPase prenylation. This leads to a reduction in LXR activity, and consequently to a decrease in the expression of LXR target genes.

Groundwater from infiltration galleries used for small public water supply systems: contamination with pesticides and endocrine disruptors

Infiltration galleries are among the oldest known means used for small public water fountains. Owing to its ancestral origin they are usually associated with high quality water. Thirty-one compounds, including pesticides and estrogens from different chemical families, were analysed in waters from infiltration galleries collected in Alto Douro Demarcated Wine region (North of Portugal). A total of twelve compounds were detected in the water samples. Nine of these compounds are described as presenting evidence or potential evidence of interfering with the hormone system of humans and wildlife. Although concentrations of the target analytes were relatively low, many of them below their limit of quantification, four compounds were above quantification limit and two of them even above the legal limit of 0.1 lg/L: dimethoate (30.38 ng/L), folpet (64.35 ng/L), terbuthylazine-desethyl (22.28 to 292.36 ng/L) and terbuthylazine (22.49 to 369.33 ng/L).

Preparação para a performance instrumental: problemáticas e estratégias: relatório de estágio

Resumo I (Prática Pedagógica) - Nesta seção do relatório de estágio pretende-se caraterizar e apresentar todos os elementos referentes à prática pedagógica, que fizeram parte do estágio do ensino especializado da música, realizado na Escola de Música do Orfeão de Leiria no ano letivo de 2013/2014. De uma forma geral, o relatório de estágio pretende colocar em evidência aspetos pedagógicos como metodologias de ensino, questões motivacionais, estilos de aprendizagem, entre outros. Durante um ano letivo completo foram analisados, com especial atenção, três alunos que foram envolvidos no estágio, construindo e modelando os seus processos de aprendizagem. Para cada um deles foi realizado, ao longo do ano letivo, 30 planos de aula, uma planificação anual e três gravações vídeo/áudio em contexto de aula. Não foi uma tarefa nada fácil de realizar, mas que trouxe inúmeros conhecimentos não só de caráter pedagógico mas também de caráter pessoal. A parte inicial desta seção será dedicada à descrição e caraterização da escola onde foi realizado o estágio, a Escola de Música do Orfeão de Leiria/Conservatório de Artes. Será feita uma pequena abordagem histórica da instituição, bem como do seu projeto educativo, dacomunidade escolar, do seu contexto sociocultural e da classe de saxofone. Será apresentada também uma caraterização dos alunos, segundo vários parâmetros. Posteriormente, serão descritas algumas das práticas educativas desenvolvidas, com os alunos, em contexto de estágio. Para tal, serão utilizados como instrumentos, não só a experiência ativa do docente/estagiário, mas também todas as gravações, planos de aulas e planificações anuais, realizados em contexto de estágio do ensino especializado. Por fim, será realizada uma reflexão crítica do desempenho como docente de saxofone no âmbito do estágio e uma conclusão de toda a seção da prática pedagógica.

Need for, demand for, and use of European Union information

The discussion and analysis of the diverse outreach activities in this article provide guidance and suggestions for academic librarians who are interested in outreach and community engagement of any scale and nature. Cases are draw from a wide spectrum and are particularly strong in the setting of large academic libraries, special collections and programming for multicultural populations. The aim of this study is to present the results of research carried out regarding the needs, demand and consumption of European Union information by users in European Documentation Centres (EDC). A quantitative methodology was chosen based on a questionnaire with 24 items. This questionnaire was distributed within the EDC of Salamanca, Spain, and the EDC of Porto, Portugal, during specific time intervals between 2010 and 2011. We examined the level of EU information that EDC users possess, and identified the factors that facilitate or hinder access to EU information, the topics most demanded, and the types of documents consulted. Analysis was made of the use that the consumer of European information makes of databases and their behaviour during the consultation. Although the sample used was not very significant owing to its small size, it is a faithful reflection of the scarce visits made to EDCs. This study can be of use to managers of EDCs, providing them with better knowledge of the information needs and demands of their users. Ultimately this should lead to improvements in the services offered. The study lies within a frame of research scarcely addressed in specialized scholarly literature: European Union information.