Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells

我们以前的研究建立了五株猕猴饲养层细胞系来支持猕猴胚胎干细胞(rESCs)的生长:一岁猴耳皮肤成纤维细胞(MESFs)、两岁猴输卵管成纤维细胞(MOFs)、成年猴卵泡颗粒成纤维样细胞(MFGs)、成年猴卵泡颗粒上皮样细胞(MFGEs),以及MESFs的克隆成纤维细胞(CMESFs).我们发现MESFs、CMESFs、MOFs和MFGs,而不足MFGEs支持猕猴胚胎干细胞(rESCs,rhesus embryonic stem cells)的生长.通过半定量PCR的方法,我们在支持性的饲养层细胞中检测到了一些基因的高表达.在本研究中,我们运用Affymetrix公司的GeneChip Rhesus Macaque Genome Array芯片来研究这五株同源饲养层的表达谱,希望发现哪些细胞因子和信号通路在维持rESCs中起到重要作用.结果表明,除MFGE外,包括GREM2、bFGF,、KITLG,、DKK3、GREM1、AREG、SERPINF1和LTBF1等八个基因的mRNA在支持性的饲养层细胞中高表达.本研究结果提示,很多信号通路在支持rESCs的未分化生长和多潜能性方面可能起到了冗余的作用.

The mobile arm support project as a test-bed for design research at the Cambridge EDC

A host of methods and tools to support designing are being developed in Cambridge EDC. These range from tools for design management to those for the generation and selection of design ideas, layouts, materials and production processes. A project, to develop a device to improve arm mobility of muscular dystrophy sufferers, is undertaken as a test-bed to evaluate and improve these methods and tools as well as to observe and modify its design and management processes. This paper presents the difficulties and advantages of using design methods and tools within this rehabilitation design context, with special focus on the evolution of the designs, tools, and management processes.

Histoarchitecture, seasonal variation and reproductive function of the neuroendocrine organ, brain of freshwater prawn, Macrobrachium gangeticum

Histoarchitecture, seasonal variation and reproductive function of the neuroendocrine structure, brain of freshwater prawn Macrobrachium gangeticum were studied. Three types of NSCs - 'B', 'C' and 'D' were found to be concentrated in four groups in brain. These cells showed larger diameters and higher activity during breeding season. In case of females, the 'C' cells were more active during vitellogenic period. Brain extracts were found to induce gonadal maturation of both males and females.

Report of the final workshop on education, capacity development and monitoring in support of Bar Reef Marine Sanctuary (BRMS) management, Kalpitiya, Sri Lanka, 23 May, 2015

Objectives of the workshop included: presentation of research to stakeholders; briefing of stakeholders on project objectives, contents and activities; to get stakeholder perceptions and suggestions.

Hard days and nights: hardships at work, a lack of support at home, and little or no social security combine to create a bleak future for women fishsellers in Mangalore, India

Mangalore is a port city situated in the west coast state of Karnataka in India. The city hosts both large-scale and small-scale fisheries along its coastline. Traditionally, fishermen catch the product and sell it at a daily auction in the harbour to women vendors, who thereafter transport the goods to the market for commercial sale. The trade starts early in the morning, when the fishermen return to the harbour from their nightly fishing.