Contribuição ao entendimento da patogenia da sepse

The most frequent cause of vasodilatory shockis outcome from sepsis, a systemic inflammatory response to infection, characterized by hypotension, hyporeactivity to the catecholamines and disseminated intravascular coagulation. The commonest cause of sepsis has reported to be infection with Gram-negative bacteria, typically E. coli, resulting in the release of lipopolysaccharide (endotoxin) from the bacterial outer membrane during autolysis or death of these microorganisms, with the involvement of many mediators, including nitric oxide. Later it was found that plasma levels of vasopressin in sepsis patients were abnormally low and observed that some patients with advanced septic shock were extremely sensitive to the activity actions of exogenous vasopressin.

Ensaios biológicos para avaliação de segurança de produtos cosméticos

Despite efforts to the contrary, some cosmetic products can cause undesirable side effects in the users. These may often be due to individual factors or inappropriate use of the product. Thus, biological assays to assess the safety of a new cosmetic must precede its being placed on the market. Historically, these tests have always been carried out in vivo, in animals, since such tests can be used to evaluate many of the potential risks, such as irritation, allergy or systemic effects; but, recently, some research centers have been adopting in vitro alternatives, in order to replace the animal tests. This review emphasizes the need to employ biological assays to test the safety of cosmetic products, and reviews the main in vivo and in vitro tests used, focusing on the need to develop and use alternatives to the in vivo assays of product safety, so as to offer the consumers the maximum safety with the least possible risk, while ensuring the best conditions of use of the product.

Determinação da resistência de Staphylococcus aureus: Um desafio?

The aim of this study was to identify the resistance profile of Staphylococcus aureus strains, in relation to induced clindamycin resistance, and to detect oxacillin resistance by the routine phenotypic methods. The strains were isolated from nasal or lingual swabs taken from healthy adult carriers with no medical history of hospitalization or antibiotic treatment. Eighteen strains were distinguished by the different patterns generated by pulsed field gel electrophoresis (PFGE). Four (22.2%) of these showed sensitivity to clindamycin by the conventional antibacterial susceptibility test, but demonstrated inducible resistance to it by the D-test. One strain (5.6%) was characterized as borderline oxacillin-resistant S. aureus (BORSA), and another (5.6%) as CA MRSA (community-associated methicillin-resistant Staphylococcus aureus). Both of these strains were shown to be cefoxitin susceptible by the disk diffusion test. The polymerase chain reaction (PCR) failed to detect the mecA gene in this last strain and it was thus classified as BORSA. These results show the importance of incorporating the D-test into the routine lab tests for S. aureus inducible clindamycin resistance and also of including the cefoxitin resistance test among the phenotypic methods for MRSA characterization.

Multiplex PCR use for Staphylococcus aureus identification and oxacillin and mupirocin resistance evaluation

Oxacillin-resistant Staphylococcus aureus represents a serious problem in hospitals worldwide, increasing infected patients' mortality and morbidity and raising treatment costs and internment time. In this study, the results of using the Multiplex PCR technique to amplify fragments of the genes femA (specific-species), mecA (oxacillin resistance) and ileS-2 (mupirocin resistance) were compared with those of tests conventionally used to identify S. aureus isolates and ascertain their resistance to drugs. Fifty S. aureus strains were isolated from patients receiving treatment at UNOESTE University Hospital in Presidente Prudente, SP, Brazil. The 686 bp fragment corresponding to the gene femA was amplified and detected in all the isolates. On the other hand, the 310 bp fragment corresponding to the mecA gene was amplified in 29 (58%) of the isolates. All of the isolates showed sensitivity to mupirocin in the agar diffusion test, which was corroborated by the lack of any amplicon of the 456 bp fragment corresponding to the ileS-2 gene, in the PCR bands. The conventional tests to identify S. aureus and detect resistance to oxacillin and mupirocin showed 100% agreement with the PCR Multiplex results. The use of techniques for rapid and accurate identification of bacteria and assessment of their resistance may be valuable in the control of infection by resistant strains, allowing the rapid isolation and treatment of an infected patient. However, the results demonstrate that traditional phenotypic tests are also reliable, though they take more time.

Litogeoquímica E aspectos Petrogenéticos dos basaltos da Província Magmática do Paraná na porção centro-norte do Estado de São Paulo

The petrographic and geochemical characterization of flood basalts of Serra Geral Formation are here presented. The investigated areas are situated in four different regions of São Paulo state: Jaú, Ribeirão Preto, Franca and Fernandópolis. They represent almost the total area of outcrops of basalts in the São Paulo State. The petrographical data reveals that these rocks are constituted mainly by plagioclase (30-40%), pyroxenes, augite and pigeonita (20-30%) and magnetite (5-15%), and show a intergranular texture and its varieties intersertal, hialophitic and pilotaxitic. The geochemical data show a basic and tholeiitic affinity of the studied basalts, with high-Ti content (TiO2 > 1.8%), typical of the northern region of Paraná Basin. Three different magma-types were recognized: Paranapanema, Urubici and Pitanga. The first magma-type is concentrated in the Fernandópolis region, the second in the Franca region, and the Pitanga occurs in the Ribeirão Preto and Jaú regions. The distribution patterns of these magma-types and the detailed study of geochemical data showed that they are, probably, generated by a melting of a continental lithospheric mantle.

Índice de Qualidade da Água (IQA) do reservatório Tanque Grande, Guarulhos (SP): Análise sazonal e efeitos do uso e ocupação do solo

The Tanque Grande Reservoir is located at the Serra da Cantereira, Guarulhos (SP), and provides 3% of the municipal drinking water. In the last years, the urban zone has expanded towards the reservoir and has significantly altered the land use. In this work, water samples from three stations were monitored from August/2007 up to July/2008 in terms of the Water Quality Index (IQA) adopted by CETESB. The waters were usually classified in the band Good during the study period. Nevertheless, termotolerants coliforms, BOD and total phosphorus have exceeded the legal limits several times. Such occurrences may be related to the economical activities at the sub-watersheds in addition to changes in the land use of the surroundings of the reservoir.

Análise morfotectônica da Bacia do rio Santo anastácio, sp, através de parâmetros fluviomorfométricos e de registros paleossísmicos

Fluvial morphometry is method of great value regarding neotectonic analysis of extensive areas because streams, besides representing one of the main agents in the relief modeling, quickly adjust their thalwegs to even the most gentle crustal deformations. The purpose of this paper is to present the application of some morphometric techniques in the Santo Anastácio River hydrographic basin in order to identify recent tectonic deformations. Additionally, we show liquefaction structures identified in the basin and interpreted here as seismites linked to ancient earthquakes (magnitudes greater than 5). The morphometric survey includes longitudinal stream profile analysis, RDE (declivity versus stream length) index application, and the sinuosity study of the Santo Anastácio River channel. Geologic substrate comprises Cretaceous siliciclastic rocks of the Caiuá and Bauru groups, locally covered by Cenozoic sediments (alluvial plains, terrace deposits, colluvial aprons, as well as in situ regoliths). Considering that liquefaction structures affect dark clay layers of 32.340±320 year BP age (14C dating), a lower age limit for the ancient earthquake is triggered in that region. The authors believe that neotectonic understanding of the Western São Paulo State Plateau is important to the geologic and geomorphologic evolution of that landscape and to the consequent territorial planning and occupation.

Influência do sistema de preparo e manejo de um latossolo vermelho nas suas propriedades físico-hídricas

The intensive use of the land for agricultural propose causes a series of modifications in attributes, which can take to soil degradation. In this context, the main goal of this research was to evaluate the influence of the soil tillage systems and management on its physical and hydric characteristics. The evaluations were carried out in July of 1999, at experimental plots of a Latossolo vermelho, a clay oxisoil, in the Faculdade de Engenharia Agrícola of the UNICAMP, at the county of Campinas, state of São Paulo. These plots were managed with the following treatments, along a period of eight years: no-tillage, chisel ploughing, conventional system with disk ploughing and revolving hoe. The evaluated physical and hydric parameters of the soil were: soil bulk density, particle density, total porosity, macro-porosity, micro-porosity, soil-water retention curve, hydraulic conductivity and basic infiltration. Significant differences were observed between the treatments on soil bulk density, infiltration, total porosity, macro-porosity and the micro-porosity. The chisel ploughing and no-tillage systems presented the higher values of soil bulk density; nevertheless in these conservationist systems were observed the higher values of basic infiltration.

Contexto geológico e estrutural do Maciço Rio Apa, sul do Cráton amazônico - MS

Rio Apa Massif crops out in the Mato Grosso do Sul state and corresponds to the southeastern portion of the Amazonian Craton dominantly Paleoproterozoic in age. Rio Apa Complex is oldest and it is composed mainly by migmatitic orthogneisses, beyond amphybolites, tonalities and granodiorite. Alto Tererê Group is composed by schists, biotitemuscovite gneisses and micaceous quartzites generally rich in garnets, beyond metabasic rocks of low amphibolite facies. The Amoguijá Group is constituted by Alumiador Intrusive Suite, which is represented by a sieno to monzogranitic batholith and Serra da Bocaina Volcanic Suite composed of volcanoclastic rocks of alkali riolites to monzoriolites compositions and pyroclastic products. Overlaying towards East and South occurs Neoproterozoic metasedimentary rocks from the Paraguai Folded Belt (Cuiabá, Corumbá and Jacadigo Groups - Urucum Formation). Structural-metamorphic framewok is identified by five deformational phases but the actual tectonic and metamorphic structure shows the superposed tectonic array of the Paraguai Folded Belt. Rocks from Rio Apa Complex, Alto Tererê Group and Amoguijá Group record an older structural evolution defined by (Dn-1 and Dn). The deformational phases (Dn+1 and Dn+2) are visible mainly in rocks of Paraguai Folded Belt beyond the last deformation (Dn+3) that imprints all sequences.

Rochas ornamentais e de revestimento do sudoeste do estado do mato grosso

Geological, petrographical, chemicals and technological studies of rocks of the southwest region of Mato Grosso State, Brazil have the purpose of qualification and application of these rocks as dimension stones. The first objective is to enlarge the offering of new esthetic varieties that show a standardization of technologic patterns, adequate characterization and classification assuring the correct specification of the rocks in relation to the use as dimension stones. Ten rock types are recognized for dimension stones, including granites, diabases, gneisses and amphibolites grouped by its colors in red, grey and black types and showing economic potential of plowing. The results obtained in the technological essays show that the rock parameters are sited inside the standard limits established by technical rules and obey satisfactorily the limit values fixed for granites in internal and external covering uses.

Diagnóstico da qualidade da água na microbacia do córrego recanto, em americana, no estado de São Paulo

The environmental degradation observed in the Piracicaba, Capivari and Jundiaí watershed has been one of the principal preoccupations of the environmental agencies in the state of São Paulo, Brazil. In this context, there is inserted the Americana county which is cut by streams of unsuitable quality for the human consumption and other uses. The main goal of the present work was evaluated the water quality of the Recanto Creek, affluent of the Quilombo stream, at the Americana county, state of São Paulo, Brazil. The research was developed in the period from March of 2007 to March of 2008, when it was measured the flow and the following physical-chemical water quality parameters: temperature; turbidity; pH; dissolved oxygen (OD); phosphorus (P); ammonia nitrogen (NH 4); nitrate (NO 3); chemical demand of oxygen (DQO), besides total coliforms. The results demonstrated a variation of the flow from 34.3 to 375.2 L s -1, during the evaluated period. The parameters dissolved oxygen, phosphorus, ammonia nitrogen and nitrate presented values out of the limits recommended for rivers of class 3, like the Recanto Creek, suggesting water pollution due to the organic matter disposal.

Avaliação preliminar do potencial turístico do município de rifaina (SP) e os impactos decorrentes do uso público de seus atrativos paisagísticos

This paper attempts to assess both the touristic potential of scenic attractions and the environmental impacts resulting from the public use of such attractions in the Rifaina Municipality, State of São Paulo, Southeastern Brazil. In the recent past, this municipality had its economy concentrated in rural activities (livestock, corn, rice and bean crops) and in the ceramic industry (production of bricks, tiles and shackles),which was largely developed in the floodplain of the Rio Grande before the construction of the Jaguara Power Plant Dam. The impacts resulting from touristic activities were evaluated by applying the VIM - Visitor Impact Management method, with emphasis on biophysical indicators. The main touristic attractions analyzed were: (1) Rifaina artificial beach; (2) Jaguara Reservoir; (3) Morro do Chapéu (scenic hill); (4) Touristic railroad; and (5) Cuestas cliffs. Attractions (1) and (2) are the most exploited ones, including, in the second case, nautical sports, sportive and craft fishing, and summer lodging. Although currently disabled, the touristic railroad presents potential conditions to be re-explored. Attractions (3) and (5), despite keeping high landscape and ecological ecotouristic potential, are the least visited places. In the present paper, a preliminary characterization of the major issues of tourism industry in the Rifaina municipality is made, including suggestions for the sustainable exploitation of tourism resources. The final results of the environmental impact evaluation of the tourism activities include small impacts in attractions (4) and (5) and moderate impacts to the three first attractions. In order to keep the sustainable touristic potential for that municipality this study proposes the improvement of the following measures: to foster research projects on technical or scientific aspects of the touristic attractions, improvement of tourism infrastructure and the creation of an Environmental Protection Area (APA) of the cuestas' scarps.

Variação das características hidrosedimentares e geomorfologia do leito do Rio Ivaí - PR, em seu curso inferior

In the present study were observed changes in morphology of the bed of the lower course of the river Ivaí, made by bathymetric surveys, granulometric analysis and measures the flow velocity. Changes made considerable changes in relation to flow and bad load. The study was conducted in the last 110 km of the river Ivaí, between the municipalities Tapira and Icaraíma - PR. In this section the channel flows with meandering pattern, embedded in sandstone Caiuá and sediment modern of its flood plain. This is the only major river in the state that so far has no modifications, making it an important site for studies of fluvial geomorphology.

Acesso a medicamentos essenciais em farmácias e drogarias do Município de Araraquara

A survey to determine the availability of 20 essential medicines for the diseases with highest prevalence in primary health care was conducted in the city of Araraquara. The presence and the price of these medicines in private sector pharmacies and drugstores of the city were recorded. Two forms, recommended by the WHO, were used in the survey, one for availability and the other for prices. The drugs most commonly available in pharmacies and drugstores were: propranolol (90.5%), captopril (96%) and ranitidine (96%), while the least available were ferrous sulfate (27%), beclomethasone (33.8%) and ibuprofen (41.9%). The drugs that showed the greatest variation among the prices charged were: propranolol (97.1%), hydrochlorothiazide (96.4%) and glibenclamide (95.0%), while the least variable were salbutamol (30.8%) and trimethoprim-sulfamethoxazole (30.2%). Generic drugs, which were half (10) of those assessed, had the lowest prices. The indicators of access, referring to the ability of patients to acquire drugs for the treatment of major diseases at the primary health care level, showed that no establishment stocked all 20 essential drugs and that wide variations existed in their prices, undermining their availability to drug users, whose only sources are pharmacies and drugstores. These data demonstrate the importance of the popular pharmacy to improve the access to medicines, by lowering the cost and increasing the availability of the items selected for the National List of Essential Drugs (RENAME).

Aplicação da metodologia de superfície de resposta na remediação de um solo arenoso contaminado artificialmente com cobre

Soil flushing is an alternative remediation technology for soils contaminated with heavy metals, which the main contaminant removing process consists in percolating an extraction solution. This work aimed to use the response surface methodology to point out combinations among the parameters of the extraction solution (Na2EDTA concentration, volume e pH) in order to reduce the concentration of copper in a sandy soil to risk levels lower than the intervention levels for exposure scenarios adopted by the Environmental Company of Sao Paulo State. Thus, a series of tests in leaching columns were carried out using a Fluvisol artificially contaminated (1257,3 mg kg-1). The tests were conducted in triplicate and setup a central composite rotatable design with 15 different parameters combinations of the extraction solution and one replicate in the center point. Using 5% significance level, the adjusted model (R2 = 0,98) indicated combinations of Na2EDTA concentration, pH and volume of the extraction solution which allow reduction of copper concentration below levels reported by environmental agency of Sao Paulo State for industrial, residential, agricultural or maximum exposure scenarios.