CO_2浓度升高对春小麦不同生育时期抗氧化系统的影响

利用开顶式气室研究了CO2浓度升高(550μmol/mol)对小麦(Triticum aestivumL)孕穗期和开花期抗氧化系统的影响。结果表明,高浓度CO2下,小麦叶片外渗电导率和丙二醛的含量下降,说明膜脂过氧化程度有所降低;虽然O2产生速率和H2O2含量2个时期相比开花期大于孕穗期,但是两者均低于对照,说明高CO2浓度下活性氧产生减少;SOD,POD,CAT的活性与对照相比有所增加并达到显著或极显著水平;随着CO2浓度的升高,ASA和Car含量也随之增加,但是随着生育期的推移,两者含量均有不同程度的减少;最终生物量和籽粒产量分别增长18%和14%,说明CO2浓度升高有利于减轻小麦的氧化损伤,促进植物生长。

不同光强下核桃楸、水曲柳和黄菠萝的光合生理特征

研究辽东山区次生林3种树种幼苗(核桃楸、水曲柳和黄菠萝)对生长光环境的适应性,在生长季测定4种光强下(全光、60%透光、30%透光、15%透光,分别记为FI,II,LI和WI)树种幼苗叶片气体交换参数、净光合速率、单位叶面积叶绿素含量和比叶重(LMA)。结果表明:随着光强的减弱,3个树种的光补偿点(LCP)、光饱和点(LSP)、最大光合速率(Pmax)、暗呼吸速率(Rd)、类胡萝卜素(Car)、LMA、单位叶鲜质量(LFA)均呈下降趋势;全光下单位叶面积的叶绿素Chl含量均低于弱光处理。本研究结果支持水曲柳在幼苗时期具有一定耐荫性的观点。树种生理生态指标的可塑性分析表明:黄菠萝能适应更宽的光强幅度,且黄菠萝的需光性大于核桃楸。不同光环境下3个树种的生理生态变化特征对解释森林生态系统内树种的共生和演替具有重要意义。

不同海拔长白山岳桦的生理变化

通过分析长白山国家级自然保护区内不同海拔(A1:1700 m,A2:1800 m,A3:1900 m,A4:2000 m,A5:2050 m)梯度岳桦叶片中各种生理指标含量的变化,探讨了林线树木适应高山环境的生理机制。结果表明:随着海拔的升高,比叶面积(SLA)显著减小,A5与A1相比下降了35.90%,差异达到显著水平;叶绿素含量随海拔梯度升高而降低,但叶绿素a/b比值(Chla/Chlb)和Car的相对含量(Car/Chl)随海拔梯度升高而增加;在海拔1900 m左右,MDA含量和MP均处于最低水平,各种酶的活性均为最低;当海拔超过2000 m,接近森林分布的界限时,MDA含量和MP升高,并达到最大值,各种酶的活性都出现了一定程度的下降。综合本次研究表明,在海拔1900 m比较适合岳桦的生长;海拔超过2000 m,岳桦体内生理抗性下降,不利于岳桦的生长发育,因此高海拔限制了岳桦的分布。

Micellization and reversible pH-sensitive phase transfer of the hyperbranched multiarm PEI-PBLG copolymer

A novel, hyperbranched, amphiphilic multiarm biodegradable polyethylenimine-poly(gamma-benZyl-L-gluta- mate) (PEI-PBLG) copolymer was prepared by the ring-opening polymerization of gamma-benzyl-L-glutamate-N-car-boxyanhydride (BLG-NCA) with hyperbranched PEI as a macroinitiator. The copolymer could self-assemble into core-shell micelles in aqueous solution with highly hydrophobic micelle cores. As the PBLG content was increased, the size of the micelles increased and the critical micelle concentration (CMC) decreased. The surface of the micelles had a positive potential. The cationic micelles were capable of complexing with plasmid DNA (pDNA), which could be released subsequently by treatment with polyanions. The PEI-PBLG copolymer formed unimolecular micelles in chloroform solution. ne pH-sensitive phase-transfer behavior exhibited two critical pH points for triggering the encapsulation and release of guest molecules. Both the encapsulation and release processes were rapid and reversible. Under strong acidic or alkaline conditions, the release process became partially or completely irreversible.

Synthesis and characterization of novel bipolar PPV-based copolymer containing triazole and carbazole units

Two new blue light-emitting PPV-based conjugated copolymers containing both an electron-withdrawing unit (triazole-TAZ) and electron-rich moieties (carbazole-CAR and bicarbazole-BCAR) were prepared by Wittig condensation polymerization between the triazole diphosphonium salt and the corresponding dialdehyde monomers. Their structures and properties were characterized by FT-IR, TGA, DSC, UV-Vis, PL spectroscopy and electrochemical measurements. The resulting copolymers are soluble in common organic solvents and thermally stable with a T-g of 147degreesC for TAZ-CAR-PPV and of 157degreesC for TAZ-BCAR-PPV. The maximum photoluminescence wavelengths of TAZ-CAR-PPV and TAZ-BCAR-PPV film appear at 460 nm and 480 nm, respectively. Cyclic voltammetry measurement demonstrates that TAZ-BCAR-PPV has good electrochemical reversibility, while TAZ-CAR-PPV exhibits the irreversible redox process. The triazole unit was found to be an effective pi-conjugation interrupter and can play the rigid spacer role in determining the emission colour of the resulting copolymer.

Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation

Insulin-like growth factor-binding protein (IGFBP)-3 is the major insulin-like growth factor (IGF) carrier protein in the bloodstream. IGFBP-3 prolongs the half-life of circulating IGFs and prevents their potential hypo-glycemic effect. IGFBP-3 is also expressed in many peripheral tissues in fetal and adult stages. In vitro, IGFBP-3 can inhibit or potentiate IGF actions and even possesses IGF-independent activities, suggesting that local IGFBP-3 may also have paracrine/autocrine function(s). The in vivo function of IGFBP-3, however, is unclear. In this study, we elucidate the developmental role of IGFBP-3 using the zebrafish model. IGFBP-3 mRNA expression is first detected in the migrating cranial neural crest cells and subsequently in pharyngeal arches in zebrafish embryos. IGFBP-3 mRNA is also persistently expressed in the developing inner ears. To determine the role of IGFBP-3 in these tissues, we ablated the IGFBP-3 gene product using morpholino-modified antisense oligonucleotides (MOs). The IGFBP-3 knocked down embryos had delayed pharyngeal skeleton morphogenesis and greatly reduced pharyngeal cartilage differentiation. Knockdown of IGFBP-3 also significantly decreased inner ear size and disrupted hair cell differentiation and semicircular canal formation. Furthermore, reintroduction of a MO-resistant form of IGFBP-3 "rescued" the MO-induced defects. These findings suggest that IGFBP-3 plays an important role in regulating pharyngeal cartilage and inner car development and growth in zebrafish.

不同海拔微孔草抗氧化系统的比较研究

对生长在3个不同海拔自然生境下微孔草（Microula sikkimensis）叶中的抗氧化系统进行了比较研究.结果表明,生长在高海拔的微孔草叶中抗氧化酶类SOD、POD和CAT活性比生长在低海拔的活性高,其中大通牛场的微孔草叶中3种酶活性最高;APX的活性随海拔的升高而升高,且低海拔地区西宁的APX活性极显著低于高海拔地区大通和海北站（P〈0.01）;作为非酶抗氧化系统物质之一的抗坏血酸（ASA）含量随海拔的升高而降低.高海拔地区微孔草叶中可溶性糖含量极高（P〈0.01）;可溶性蛋白含量随海拔升高呈V字形变化.光合色素Chl a、Chlb和Car的含量均随着海拔升高而增加,Chl a/b比值随海拔升高而降低.MDA的含量随海拔升高有增加的趋势,西宁和大通的相比较,MDA含量差异显著（P〈0.05）,说明微孔草叶细胞膜脂过氧化程度随海拔升高加剧.生长在不同海拔高度的微孔草对不同海拔高度环境变化具有相应的生理适应性和抗氧化策略.

高寒草甸植物抗氧化系统对长期增强UV-B辐射的响应

通过研究自然条件下模拟平流层臭氧破坏5%时近地表面增加的太阳UV-B辐射对高寒草甸4种典型植物（矮嵩草Kobresia humilis、垂穗披碱草Elymus nutans、麻花艽Gentiana straminea和鹅绒委陵菜Po-tentilla anserina）的抗氧化系统的影响表明,尽管各植物的抗氧化系统组分变化不同,但4种植物的膜脂过氧化程度没有加剧,长期增强UV-B辐射没有对膜系统造成损伤。在自然长期增强UV-B条件下,4种植物的膜脂过氧化产物——丙二醛（MDA）含量与对照相比无显著差异。垂穗披碱草、鹅绒委陵菜的谷胱甘肽（GSH）含量增加,麻花艽的超氧化物歧化酶（SOD）活性与鹅绒委陵菜的过氧化氢酶（CAT）活性上升,同时麻花艽的类胡萝卜素（Car）含量亦显著增加。可见这些植物已能很好地适应UV-B强辐射,其抗氧化能力除了与抗氧化系统各组分的协同作用有关外,也可能与种的适应性有关。

长期增强UV-B辐射对高寒草甸植物光合速率和抗氧化系统的影响

研究了在野外自然条件下，长期增强UV-B辐射对高寒草甸3种典型植物矮嵩草（Kobresia humilis）、垂穗披碱草（Elymus nutans）和钉柱委陵菜（Potentilla saundersiana）光合放氧速率、光合色素和抗氧化系统的影响。结果表明：长期增强UV-B辐射对3种植物的净光合速率没有明显影响。增强UV-B辐射下，3种植物的叶绿素含量变化不同，Chla／b值，类胡萝卜素（Car）含量，Car／Chl值与对照相比都有升高，说明植物叶片的光合能力、吸收紫外线的能力增强以及忍受逆境能力均有增强，从而产生光保护，有利于光合作用正常进行。由于3种植物膜脂过氧化程度的不同及SOD活性的普遍抑制，植物经受了氧化胁迫。垂穗披碱草叶片GSH含量显著七升，矮嵩草的形态矮小及钉柱委陵菜GSH含量与POD活性显著上升，都能减轻它们所经受的氧化胁迫，使光合器官免受损伤。所以，这些保护性色素的积累和抗氧化系统内部的协同作用可能是高寒草甸植物光合作用正常进行的重要原因。

Comparative analysis of allantoin quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in Nitraria tangutorum Bobr. Seed by HPLC-APCI-MS and CE

This paper describes the simultaneous determination of allantoin, quercetin, and 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA) in Nitraria tangutorum Bobr seed by HPLC-APCI-MS and CE (capillary electrophoresis) methods. The final optimized chromatographic conditions were investigated in a reversed-phase Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m). A seventeen-minute gradient elution, (A: aqueous acetonitrile 20% (v/v); B: aqueous acetonitrile 60% (v/v); C: pure acetonitrile 100%) at a flow rate of 1.0 mL/min was selected for the separation of three natural products with diode array detection (DAD) at 220 nm. A CE experiment was carried out in a fused silica capillary with 32 mmol/L boric acid (pH 10), 32 mmol/L SDS and acetonitrile (10.0%, v/v). The applied potential and temperature was, respectively, set at 19 kV and 25 degrees C. After development, the validation was performed in parallel for HPLC and CE, with the same standards and sample to avoid differences due to the manipulation. The validation parameters of both techniques were adequate for the intended purpose.

冗余度机器人作业区域轨迹的自动规划

机器人化是提高工程机械施工控制自动化的关键问题。对于工程机械机器人 ,特别是具有空间冗余度的工程机械机器人 ,其执行机构末端的轨迹规划的自动实现研究对提高其自动化程度有着重要意义。本文以泵车为例 ,提出了一种泵车布料机构的浇筑过程的自动轨迹规划算法 ,该算法通过将其所浇筑区域离散成浇筑点集 ,对两浇筑点的轨迹利用冗余度机器人学的最小关节范数法 ,并通过在关节速度和加速度非连续之处采用平滑或连续处理 ,从而实现了泵车布料机构浇筑过程的自动轨迹规划。

混凝土泵车布料机构自动浇筑及过程仿真

机器人化是提高工程机械施工控制自动化的关键问题·通过将泵车布料机构的浇筑区域离散为浇筑点集 ,对两浇筑点内轨迹利用冗余度机器人学的最小关节范数法的轨迹规划 ,并将两相临浇筑段自动连接和自动协调 ,从而实现了混凝土泵车布料机构浇筑过程自动轨迹规划·

汽车零件精段自动生产线机械手设计

在高温、多粉尘、多有害气体的危险环境中,工业机械手的应用就尤为显得重要,我们设计的液压组合式机械手特点是巧妙地使油缸远离手爪,以防油液受热使油的黏度下降。能在振动、油污等条件下稳定和可靠的工作,在锻造水压机生产流水线中,代替人手来搬运,装卸和操作,不但减轻了工人的劳动强度还大大的提高了劳动生产率。

汽车混流总装线投产排序优化研究

汽车混流总装线上交替装配不同型号的汽车产品,不同型号的汽车在同一装配工位上装配的零部件可能不同,如果不对车型的投产顺序加以优化排序,连续投入一种相同的车型,会使同一个工位的零部件消耗率严重不均衡。为了使各装配工位的零部件消耗率达到均匀化和平准化,本文采用模拟退火优化算法,对车型上线序列进行优化,最后的实例验证表明算法是可行的。

一种基于模糊petri网专家系统的面向对象方法设计

简要介绍了模糊petri网以及模糊产生式规则,给出了基于模糊petri网的专家系统的框架设计,并提出了模糊产生式规则和模糊petri网的详细设计,根据本设计方案开发了汽车变速箱故障诊断专家系统,证明设计方案简洁高效,扩充性和实用性好。