Use of HEPG2 cells to assay the safety of cosmetic active substances

The importance of this study is based on the need to obtain simple and efficient in vitro models to predict the in vivo toxicity of cosmetics, aiming not to use animals as experimental model. Here, we proposed the use of HepG2 cells, which are widely applied to simulate the hepatic function of the human organism in vitro. This cell line was chose since recent studies have shown that the liver is potentially the most frequently targeted organ by cosmetic ingredients, and beyond that, considering the widely application of in vitro assays to test the cutaneous permeation of cosmetic products, including the assays applying modified Franz cells, this technique becomes indispensable. Three different cosmetic active substances were used, and the toxicity to HepG2 cells was assessed by the MTT method. The treatment with hyaluronic acid showed no toxicity to HepG2 cells. Treating the cells with P. guajava L. extract were verified that increasing the amount of the extract in the media, the cellular viability decreased, and finally, the treatment of alpha-lipoic acid showed a cytoprotective effect in relation to the treatment with propylene glycol. The study demonstrated the suitability in using HepG2 cells to assess the safety of cosmetic active substances, helping in the prediction of if the substance could be hepatotoxic if could reach the bloodstream

Avaliação dos benefícios da adoção de boas práticas de manejo no bem-estar de bezerros leiteiros

Pós-graduação em Zootecnia - FCAV

Avaliação dos efeitos da terapia fotodinâmica com hipericina-glucamina no tratamento da doen ça periodontal induzida em ratos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

TLR 2, TLR 4, ROS, óxido nítrico, P38 e IKK em células mononucleares de cães com leishmaniose Visceral tratadas com o imunomodulador P-MAPA: Larissa Martins Melo. -

Pós-graduação em Ciência Animal - FMVA

In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus

Aim To evaluate the inflammatory process induced by Biodentine and mineral trioxide aggregate (MTA) in rat subcutaneous tissues. Methodology A polyethylene tube filled with Biodentine (n = 20) or MTA (n = 20) was placed into the dorsal subcutaneous of forty male rats; in the control group (CG; n = 20), empty tubes were implanted. After 7, 15, 30 and 60 days, the polyethylene tubes surrounded by connective tissue were fixed and embedded in paraffin. The number of inflammatory cells was estimated in HE-stained sections; numerical density of interleukin-6 (IL-6)-immunolabelled cells was also performed. The differences amongst the groups were analysed statistically by Tukey's test (P ≤ 0.05). Results A high number of inflammatory cells and IL-6-positive cells were observed at 7 days, in all groups; however, in the Biodentine group, the number of inflammatory cells and IL-6-immunolabelled cells was significantly higher (P ≤ 0.05) in comparison with the other groups at 7 and 15 days. In the capsules of animals from all groups, a gradual and significant reduction (P ≤ 0.05) of these parameters was seen over time. At 60 days, the capsules exhibited numerous fibroblasts and bundles of collagen fibres; in addition, the number of IL-6-positive cells was not significantly different amongst Biodentine, MTA and control groups. Conclusions There was a significant regression in the inflammatory reaction in the capsules indicating, therefore, that Biodentine is a biocompatible material.

Sensores eletroquímicos à base de nanomateriais carbonáceos e catalisadores biomiméticos para determinação de tetraciclina em diferentes tipos de amostras

Pós-graduação em Química - IQ

Variação sazonal das variáveis físicas e químicas em riachos de duas bacias da região centro-sul do estado do Paraná, sul do Brasil

The present study was developed in two streams from the two different drainage basins located in the mid-southern region of Paraná state. In each stream were evaluated some physical and chemical parameters in open (without streamside vegetation) and shaded (with streamside vegetation) segments. Monthly samplings were carried out from January to December 2007. The following limnological parameters were measured: water temperature, specific conductance, oxygen saturation, pH, turbidity and current velocity. In the same stream were not observed differences among segments from the drainage basins, and nominal values and seasonal pattern have been very similar between open and shaded ambient. A Principal Component Analysis (PCA) showed small divergence between streams and segments, with separation of groups with base sampling dates. The results suggest that the region is homogeneous according to physical and chemical water conditions, therefore, stronger influenced for time factor, and diverging seasonal periods associated a climatic region condition.

Distance exercised during submaximal training on race winnings for Thoroughbred racehorses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da produção de citocinas, expressão gênica e análise funcional de células SHED expostas ao LTA bacteriano

Pós-graduação em Odontologia Restauradora - ICT

Estudo comparativo de filmes de corantes fenotiazínicos na superfície de eletrodo de carbono vítreo e adsorvidos em titânio fosfatado e suas aplicações eletroanalíticas

Pós-graduação em Ciência dos Materiais - FEIS

Avaliação in vitro do P-cresol sobre o metabolismo oxidativo e apoptose dos neutrófilos de cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Natação e desenvolvimento motor em idade escolar

Swimming is a sport well suited to any age group, it worked properly when the individual stimuli that provides assist in human development. But water stimulation, is not something that is so easily in physical education in Brazil. Development corresponds to changes that occur throughout life, but that depends on the stimuli provided. Several studies have shown positive effects of swimming in relation to the child. This study aimed to verify if children swimmers and physical education classes have better motor development than those who do not practice the sport. Collection was done with two groups of 10 students between 9 and 11 years. As a group consisting of non-swimmers, and another formed by those that complement the physical education class with the regular practice of swimming. To develop the study was administered a battery of the proposed motor adapted Rosa Neto (2002) in which each child performed tasks corresponding to their chronological age (CA) and were only able to perform the task at a later age, when it was obtained successful in completing the task as originally proposed. At the end of the application of all statistical tests was done getting mean and standard deviation of the old motor (IM), calculated the ratio General Motor (MGQ) for the classification of the child's motor development and application of the t test with p <0.05 to observe the level of significance in the results. The results showed that the group swimmers had better agility tests which resulted in a difference between (IC) and (IM) higher compared with the non-practitioner. They also had higher average (MGQ), but these were not sufficient to classify the group into another level of development, however, by observing isolated cases, two children that practice would be a greater level of development. Through the t test was observed that the group swimmers had significant difference in balance ability. We conclude that the swimming group got positive overall...

Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ketorolac eye drops reduce inflammation and delay re-epithelization in response to corneal alkali burn in rabbits, without affecting iNOS or MMP-9

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Synthesis, Characterization, and Antifungal Activities of Amphiphilic Derivatives of Diethylaminoethyl Chitosan against Aspergillus flavus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)