Anti-inflammatory effect of MAPK phosphatase-1 local gene transfer in inflammatory bone loss

Alveolar bone loss associated with periodontal diseases is the result of osteoclastogenesis induced by bacterial pathogens. The mitogen-activated protein kinase (MAPK) phosphatase 1 (MKP-1) is a critical negative regulator of immune response as a key phosphatase capable of dephosphorylating activated MAPKs. In this study, rat macrophages transduced with recombinant adenovirus (Ad.)MKP-1 specifically dephosphorylated activated MAPKs induced by lipopolysaccharide (LPS) compared with control cells. Bone marrow macrophages from MKP-1 knockout (KO) mice exhibited higher interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α, and select chemokine compared with wild-type (WT) mice when stimulated by LPS. In addition, bone marrow cultures from MKP-1 KO mice exhibited significantly more osteoclastogenesis induced by LPS than when compared with WT mice. Importantly, MKP-1 gene transfer in bone marrow cells of MKP-1 KO mice significantly decreased IL-6, IL-10, TNF-α and chemokine levels, and formed fewer osteoclasts induced by LPS than compared with control group of cells. Furthermore, MKP-1 gene transfer in an experimental periodontal disease model attenuated bone resorption induced by LPS. Histological analysis confirmed that periodontal tissues transduced with Ad. MKP-1 exhibited less infiltrated inflammatory cells, less osteoclasts and less IL-6 than compared with rats of control groups. These studies indicate that MKP-1 is a key therapeutic target to control of inflammation-induced bone loss.

Conhecimento odontológico de adultos atendidos em uma unidade de saúde da família do município de São Carlos, Brasil

The Family Health Strategy is a primary care public policy that is becoming a decisive step towards the transformation of the Brazilian healthcare model. This study evaluated the dental knowledge among individuals who attended a Family-Health Unit (FHU) in the city of São Carlos, SP, Brazil. Methods: The evaluation was based on a questionnaire with 20 questions about oral health, prevention and development of dental caries and periodontal disease. The questionnaire was given to 168 individuals, over 18 years of age and both sexes, to fill out under the supervision of a trained dentist. After data collection, the answers contained in the original questionnaires were entered into a database built using the program Excel® (Microsoft Corporation, USA) and a descriptive statistical analysis was done. Results: 66.67% of the subjects reported having received information about dental caries and periodontal disease before attending the FHU, and dentists were cited as the main source of such information (60.71%). With regard to dental caries, 70.24% of the individuals stated that they knew about the subject, but the majority of answers revealed incorrect explanation of it. The multifactorial etiology (bacteria/sugar/poor hygiene) was not mentioned. A low percentage of subjects (24.40%) claimed to know what periodontal disease is, and of these, the highest percentage of responses was related to gingivitis (26.83%) and gingival bleeding (12.20%). Out of all subjects, 80.36% affirmed that oral hygiene is important, and among them, the main reasons cited were the maintenance of oral health (29.63%), to avoid diseases (16.30%) and to preserve and prevent disease (14.81%). Conclusion: Responses from the questionnaires revealed deficient dental knowledge among the individuals. To improve the understanding about this issue, the information obtained through the questionnaire can be used to develop educational programs that will focus specifically on the major deficiencies found.

Rua da Frente: Avenida Getúlio Vargas como contexto na prostituição em São Carlos - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Clima e produção do espaço urbano: contribuição ao estudo da Geografia do Clima no contexto das cidades de São Carlos e Marília

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Condomínios residenciais fechados: a urbanização do Grupo Encalso Damha em São Carlos - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Overproduction and properties of lipase by wild strain of Burkholderia lata LBBIO-BL02 using chicken fat

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caso de Poesia (Antônio Carlos de Brito, vulgo Cacaso)

This paper deals with characteristically anti-authoritarian aspects of Antônio Carlos de Brito’s work, alias Cacaso (1944-1987). It aims to provide evidence on his peculiar poetry writing exercise, which incorporates political and aesthetic tensions into its own language, rather than just refer to them. By anchoring in a poetics of everyday life and by being engaged in a dialogue with Brazilian literary tradition, Cacaso manages image, tone and emotion in order to instantly illuminate the impasses of his generation.

Estudo da interação entre a via genética do microRNA156/squamosa promoter-binding protein-like e brassinosteróides durante o desenvolvimento de Arabidopsis thaliana

Pós-graduação em Ciências Biológicas (Genética) - IBB

Knowledge and use of wild edible plants in rural communities along Paraguay River, Pantanal, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mites on Annonaceae species in northeast Brazil and in the state of Para

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bartonella clarridgeiae and Bartonella vinsonii subsp berkhoffii exposure in captive wild canids in Brazil

Wild canids are potential hosts for numerous species of Bartonella, yet little research has been done to quantify their infection rates in South America. We sought to investigate Bartonella seroprevalence in captive wild canids from 19 zoos in São Paulo and Mato Grosso states, Brazil. Blood samples were collected from 97 wild canids belonging to four different native species and three European wolves (Canis lupus). Indirect immunofluorescent antibody testing was performed to detect the presence of B. henselae, B. vinsonii subsp. berkhoffii, B. clarridgeiae, and B. rochalimae. Overall, Bartonella antibodies were detected in 11 of the canids, including five (12·8%) of 39 crab-eating foxes (Cerdocyon thous), three (11·1%) of 27 bush dogs (Speothos venaticus), two (8·7%) of 23 maned wolves (Chrysocyon brachyurus) and one (12·5%) of eight hoary foxes (Lycalopex vetulus), with titres ranging from 1:64 to 1:512. Knowing that many species of canids make excellent reservoir hosts for Bartonella, and that there is zoonotic potential for all Bartonella spp. tested for, it will be important to conduct further research in non-captive wild canids to gain an accurate understanding of Bartonella infection in free-ranging wild canids in South America.

Production of exopolysaccharide from rhizobia with potential biotechnological and bioremediation applications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Amblyomma yucumense n. sp (Acari: Ixodidae), a parasite of wild mammals in Southern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Does the rattle of Crotalus durissus terrificus reveal its dietary history?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)