Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects.

Transformation of cells in tissue culture results in a variety of cellular changes including alterations in cell growth, adhesiveness, motility, morphology, and organization of the cytoskeleton. Morphological and cytoskeletal changes are perhaps the most readily apparent features of transformed cells. Although a number of studies have documented a decrease in the expression of specific tropomyosin (TM) isoforms in transformed cells, it remains to be determined if the suppression of TM synthesis is essential in the establishment and maintenance of the transformed pheno-type. To address the roles of different TM isoforms in transformed cells we have examined the effects of expressing specific TM isoforms in transformed cells using a Kirsten virus-transformed cell line (ATCC NRK1569) as a model system. In contrast to normal fibroblasts, the NRK 1569 cells contain reduced levels of TM-1 and undetectable levels of TM-2 and TM-3. These cells have a rounded morphology and are devoid of stress fibers. Employing expression plasmids for TM-2 and TM-3, stable cell lines were established from the NRK 1569 cells that express these isoforms individually. We demonstrate that expression of TM-2 or TM-3 leads to increased cell spreading accompanied by the formation of identifiable microfilament bundles, as well as significant restoration of well-defined vinculin-containing focal adhesion plaques, although expression of each isoform exhibited distinct properties. In addition, cells expressing TM-2, but not TM-3, exhibited contact-inhibited cell growth and a requirement for serum.

Pieter Bruegel the Elder’s Netherlandish Proverbs and Sixteenth Century Dutch Semiotics

"In 1559, Pieter Bruegel the Elder‘s depiction of {7f2015}Netherlandish Proverbs‖ illustrated his profound understanding of the Dutch love for proverbs, their contemporary values, and appreciation for moral lessons in art forms. Depicting gestures and poses that represented proverbial phrases enabled Bruegel‘s leap from didactic labels employed by other artists to his inscription-free success of {7f2015}Netherlandish Proverbs.‖ My examination reveals that Bruegel‘s employment of gestural imagery, indicating rhetorical phrases or proverbs, was reinforced by a history of scholarly curatorship for written proverb collections, humanist interest in proverbs, and use of Dutch vernacular to bolster protonational pride"

Improving kNN multi-label classification in Prototype Selection scenarios using class proposals

Prototype Selection (PS) algorithms allow a faster Nearest Neighbor classification by keeping only the most profitable prototypes of the training set. In turn, these schemes typically lower the performance accuracy. In this work a new strategy for multi-label classifications tasks is proposed to solve this accuracy drop without the need of using all the training set. For that, given a new instance, the PS algorithm is used as a fast recommender system which retrieves the most likely classes. Then, the actual classification is performed only considering the prototypes from the initial training set belonging to the suggested classes. Results show that this strategy provides a large set of trade-off solutions which fills the gap between PS-based classification efficiency and conventional kNN accuracy. Furthermore, this scheme is not only able to, at best, reach the performance of conventional kNN with barely a third of distances computed, but it does also outperform the latter in noisy scenarios, proving to be a much more robust approach.

Phraseological features in conversational routines: An integrative model of autonomy

This paper deals with the analysis of routine formulas according to their degree of independence in discourse, with the aim of establishing a gradual line which restructures Sphere III (Corpas 1996, Alvarado 2015) — where phraseological utterances belong. The system developed by Briz and the Val.Es.Co. Group (2003, 2014) will help us segment conversation and check that phraseological utterances show various degrees of independence, both in Peninsular Spanish and in Latin American Spanish, which will make it possible to restructure Sphere III. The methodology utilized corresponds to the phraseological and pragmatic approach, and the examples were extracted from the Corpus de Conversaciones Coloquiales of Briz and the Val.Es.Co. Group (2002), and from the Corpus Preseea (2014).

On terminological figurativeness: From theory to practice

Terminologists’ interest in studying the role of metaphor and metaphorical terms in specialized communication has proliferated since the first papers addressing this issue appeared in the 1990s. However, we believe that some facets of terminological meaning still remain overlooked or merit further analysis. This paper attempts to contribute to the study of one of these facets: the figurative meaning of some compound terms used in the domain of luxury marketing and business. In order to present a systematized view of this phenomenon we will adopt some theoretical tools from the Conventional Figurative Language Theory, in order to confirm the validity of some of its postulates for compound term analysis. Next, a contrastive approach between English, Spanish and Russian compound terms will put the theoretical ideas into practice with the aim of illustrating their applied and metalinguistic potential. Some basic conclusions will be offered at the end of the paper.

Sharḥ Qānūn Āmulī : manuscript, undated

Paper.

Literatura Infantil na Educação Pré-escolar: fruição, leitura literária e compreensão leitora

Com o presente artigo, defende-se que a Educação Pré-escolar é a primeira etapa da educação básica e que a criança é um sujeito ativo na construção do seu saber. Deve por isso ser estimulada e incentivada nesse sentido pelo educador de infância, que tem um papel decisivo na instauração de um ambiente educativo promotor de aprendizagens contextualizadas, integradoras e significativas. No que ao lugar dos livros no jardim-de-infância diz respeito, assume-se também, neste artigo, que se deve proporcionar aos mais novos um contacto precoce e sistemático com livros de qualidade, sobretudo para desenvolver a fruição, a sensibilidade estética, a imaginação, o pensamento divergente e a compreensão leitora.

Graft preservation solutions in cardiovascular surgery.

Vein grafts are still the most commonly used graft material in cardiovascular surgery and much effort has been spent in recent years on investigating the optimal harvesting technique. One other related topic of similar importance remained more or less an incidental one. The storage solutions of vein grafts following procurement and prior to implantation are, despite their assumed impact, a relatively neglected theme. There is no doubt that the endothelium plays a key role in long-term patency of vein grafts, but the effects of the different storage solutions on the endothelium remain unclear : In a review of the literature, we could find 20 specific papers that addressed the question, of which the currently available preservation solutions are superior, harmless, damaging or ineffective. The focus lies on saline and autologous whole blood. Besides these two storage media, novel or alternative solutions have been investigated with surprising findings. In addition, a few words will be spent on potential alternatives and novel solutions on the market. As there is currently no randomized clinical trial regarding saline versus autologous whole blood available, this review compares all previous studies and methods of analysis to provide a certain level of evidence on this topic. In summary, saline has negative effects on the endothelial layers and therefore may compromise graft patency. Related factors, such as distension pressure, may outbalance the initial benefit of autologous whole blood or storage solutions and intensify the harmful effects of warm saline. In addition, there is no uniform consent on the superiority of autologous whole blood for vein graft storage. This may open the door to alternatives such as the University of Wisconsin solution or one of the specific designed storage solutions like TiProtec™ or Somaluthion™. Whether these preservation solutions are superior or advantageous remains the subject of further studies.

Interleukin-6-deficient mice are highly susceptible to Giardia lamblia infection but exhibit normal intestinal immunoglobulin A responses against the parasite.

In the present study, interleukin-6 (IL-6)-deficient mice were infected with Giardia lamblia clone GS/M-83-H7. Murine IL-6 deficiency did not affect the synthesis of parasite-specific intestinal immunoglobulin A. However, in contrast to wild-type mice, IL-6-deficient animals were not able to control the acute phase of parasite infection. Reverse transcription-PCR-based quantitation of cytokine mRNA levels in peripheral lymph node cells exhibited a short-term up-regulation of IL-4 expression in IL-6-deficient mice that seemed to be associated with failure in controlling the parasite population. This observation suggests a further elucidation of IL-4-dependent, Th2-type regulatory processes regarding their potential to influence the course of G. lamblia infection in the experimental murine host.

Constitutiones examinis generalis Societatis Iesu

Capitular grab. xil.

Commentarius in duos libros Aristotelis de ortu, atque interitu ... / authore Gasparo Cardillo Villalpandeo ...

Marca tip. en port.

Silua de varia lection / compuesta por el Magnifico cauallero Pero Mexia.

Lugar e impresor tomados del colofón

Sermones de sanctis / diui Vincentii ; de sanctis & in orationem dominicam sermonum opus sancti Vincentij, ordinis Praedicatorum, diuini praeconis, ad vetustorum exemplarium fidem diligenter emendatum ; cum eorum indice locupletissimo.

Capitulares grab. xil.

DVD-based distance-learning program for university entrance exams -- RCT experiments in rural Bangladesh

In contrast to the remarkable progress in developing countries in improving primary education, access to higher education in many countries remains limited, especially in rural areas where the quality of education is inadequate. We evaluate a DVD-based distance-learning program in rural Bangladesh, targeted at students aiming to take university entrance tests. We conducted two experiments: one to evaluate the effect of the distance-learning program and the second to determine the demand and price sensitivity. Our first experiment shows that the DVD-based distance-learning program has a considerable positive effect on the number of students passing entrance exams. This effect does not depend on cognitive scores, but does depend on non-cognitive attributes, indicating the importance of commitment, which is imposed through our program. In the second experiment, we offered a random subsidy to interested participants. The uptake decision is price-sensitive, although the price sensitivity is not correlated with students' past academic performance or their socio-economic status, suggesting that increasing the price should not disproportionately exclude poor students.