Não preferência para alimentação e antibiose em cultivares de amendoim a Stegasta bosquella (Chambers) (Lepidoptera: Gelechiidae)

This work aimed to evaluate the attractiveness, non-preference for feeding and antibiosis in straight and runner growth habit peanut cultivars to Stegasta bosquella (Chambers). Eight cultivars were evaluated, four of straight growth habit (IAC Tatu, IAC 22, IAC 8112 and IAC 5) and four of runner growth habit (IAC Runner 886, IAC 147, IAC 125 and IAC 503). Free-choice and no-choice feeding tests were performed, using pairs of overlapped leaf discs with 1.0 cm diameter, which were placed in Petri dishes where third instar larvae of S. bosquella were released. The attractiveness to the larvae was assessed in predetermined times, in addition to the dry mass consumed. In the antibiosis assay, the biological parameters were evaluated: period and viability of larvae, pre-pupae, pupae, and total, weight of larvae and pupae, sex ratio and longevity. None of the runner growth habit cultivars exhibited non-preference for feeding-type resistance. Among the straight growth habit cultivars, IAC 5 and IAC 22 were the least attractive and consumed in the free-choice feeding test, and IAC 5 and IAC 8112 were the least attractive in the no-choice test. The runner growth habit cultivars IAC 147 and IAC Runner 886 affected the larval survival of S. bosquella, exhibiting antibiosis-type resistance. For the straight growth habit cultivars, IAC 22 and IAC 8112 affected the larval viability, presenting antibiosis-type resistance. The straight and runner growth habit cultivars did not influence the biological parameters of weight of pupae, sex ratio and longevity of S. bosquella.

Efeito da alimentação do adulto na qualidade do endoparasitoide larval da broca comum da cana-de-açúcar

Aiming qualitative improvements in the mass rearing of Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) in laboratory, tests were conducted with adults feed before parasitism on Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae, using 14 artificial diets based on sucrose, brewer yeast and honey. The design was completely randomized with 15 treatments and five replications. The biological parameters evaluated in F1 and F2 generations were: number of adults emerged; sex ratio; adult emergence percentage; weight of pupae mass; and male and female longevity. The highest average of emerged adults were 85 in diet with sucrose 5% for F1 generation, and 91 in diet with sucrose 5% + yeast brewer 2,5% for F2; the smaller average of adult emergency were 63.60 in diet with honey 5% + yeast brewer 2.5 % for F1 generation and 66.80 in diet with honey 5% for F2. Diets that produced more females were honey 5% + yeast brewer 2.5%, showing the sex ratio of 0.82 for F1 generation, and sucrose 10% + 2.5% yeast brewer 2.5% (0.75) for F2, while sucrose 10% + yeast brewer 2.5% (0.60 for F1) and honey 2.5% (0.59) provided fewer females for F2 generation. The highest percentage of adult emergence were 93.90% with the diet containing sucrose 2.5% + yeast brewer 2.5%) and 93.89 % with honey 5% + yeast brewer 2.5%, and the lowest 81.71% (honey 5% + yeast brewer 2.5%) for F1 and 78.96 % (honey 5%) to F1 and F2, respectively. The diet with Mel 2.5% produced greater weight of pupae mass. The adult longevity did not differ significantly for all diets in F1 and F2 generations. Diets containing 5% or 10% of sucrose or honey provide, in general, improve the qualitative development of C. flavipes in mass rearing.

Determinação dos tipos de resistência a Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) em genótipos de soja

The aim of this work was to evaluate the resistance types in soybean genotypes to Spodoptera cosmioides (Walker) in laboratory. Soybean genotypes assessed were as follows: 'IAC 100'(resistance standard), 'BR16'(susceptible standard), 'Dowling', PI 227687, PI 274454, 'IGRA RA 626 RR', PI 227682, 'BRSGO 8360', 'IGRA RA 516 RR'and 'P 98Y11 RR'. Free-choice and no-choice feeding non-preference tests were done using two newly-hatched larvae per genotype or one third-instar larva per genotype in both tests. Larvae attractiveness was evaluated in different times, and at the end of the experiments the leaf area consumed was quantified. In the antibiosis test, newly-hatched larvae were individualized into Petri dishes, where leaflets of the genotypes were offered over the larval stage, and the following biological parameters were assessed: period and viability of larvae, pupae and overall (larvae + pupae), weight of larvae and pupae, sex ratio and adults longevity. Overall, in the feeding preference tests, significant differences were not found in leaf consumption among the genotypes. In the antibiosis assay, genotypes PI 227687, PI 227682 and 'IAC 100'caused 100% larval mortality and the lowest weight of larvae, ranging between 37.65 and 85.56 mg. All soybean genotypes evaluated do not exhibit feeding non-preference type resistance to S. cosmioides, and PI 227687, PI 227682 and 'IAC 100'highlighted for possessing antibiosis.

The importance of relative humidity for telenomus remus (Hymenoptera: Platygastridae) parasitism and development on Corcyra cephalonica (Lepidoptera: Pyralidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Formulations of Melia azedarach to control diabrotica speciosa (germar) (coleoptera: chrysomelidae) larvae in corn and plant enhancement

We evaluated oil and powder formulations of Melia azedarach for controlling larvae of Diabrotica speciosa (Germar) in corn and plant enhancement. Five concentrations of each formulation were evaluated and compared to fipronil (negative control) and distilled water (positive control). After treatment, the number of surviving insects (larvae, pupae, and adults), the adult body weight, the sex ratio, and the longevity were recorded, while the height, dry weight of aerial part and roots, and number of leaves of plants were measured. The oil formulation at 4.0 mL reduced the larvae population of D. speciosa similarly to the insecticide fipronil, which resulted in greater height, dry weight of the root system, and number of leaves. Powder formulation at concentrations of 40, 80, and 160 mg caused larval mortality above 80%; however, these concentrations did not prevent reduction of plant height and dry weight of aerial part. Further studies assessing the residual period of M. azedarach control against D. speciosa larvae and its phytotoxicity, which are common traits associated with azadirachtin application, are necessary to subsidize the next steps of this alternative control strategy.

Effect of melatonin intake on oxidative stress biomarkers in male reproductive organs of rats under experimental diabetes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Non-synonymous mutations mapped to chromosome X associated with andrological and growth traits in beef cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic relationship among reproductive traits in Nellore cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative study of resistance to heat in two species of leaf-cutting ants

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Life cycle of Raoiella indica (Acari: Tenuipalpidae) on ornamental plants, mostly Arecaceae

The red palm mite, Raoiella indica Hirst, has been primarily found associated with coconut and musaceous plants in the New World. However, it has also been recorded on several other palms, heliconiaceous and zingiberaceous species. This study was conducted to evaluate the suitability of different botanical families on which R. indica has been collected in the field and of arecaceous plants of the natural vegetation of the neotropics. In total, ten species of Arecaceae as well as Heliconia psittacorum [Heliconiaceae] and Alpinia purpurata [Zingiberacae] were evaluated, using coconut as a control. The study was carried out under controlled conditions (29 +/- A 0.5 A degrees C, 60 +/- A 10 % RH and photoperiod 12 h of light). Raoiella indica was able to complete immature development only on coconut, Adonidia merrillii, Ptychosperma macarthurii, H. psittacorum and A. purpurata. Duration of the immature phase (egg-adult) ranged between 21.5 days on coconut to 34.1 days on A. purpurata. Longevity was at least 50 % greater and oviposition at least 38 % higher on coconut than on other plants. Intrinsic rate of increase (r(m)) was higher on coconut (0.166) and A. merrillii (0.042), but negative on the other two plant species. Raoiella indica could not reach adulthood on any of the other ten arecaceous species considered in the study. The results suggested R. indica to be a threat to A. merrillii in addition to coconut, but not to other evaluated plants. However, complementary studies should be conducted to investigate whether the experimental procedures adopted in this study could not have prevented the mite from a better performance than it could have been under field conditions, especially in relation to Mauritia flexuosa, one of the dominant arecaceous plants in South America.

Growth and development time of subtropical Cladocera Diaphanosoma birgei Korinek, 1981 fed with different microalgal diets

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biologia comparada de populações da lagarta–do–cartucho em folhas de milho e mandioca

The fall armyworm is a pest that feeds on various botanies species. The objective of this experiment was to study the biology of this pest in corn and cassava leaves. Caterpillars were collected in area under cultivation with cassava and maintained on artificial diet for two generations. Under controlled conditions in a climatic chamber (B.O.D) in the laboratory (25 degrees, 60 +/- 10% RH and photophase 14 hours) were evaluated daily 50 caterpillars in corn treatments and 50 in cassava, where duration and viability of the larval phase and pupal, weight of pupas were observed after 24 hours, deformation percentage of pupas and adults, longevity, fecundity and total life cycle. The viability of larvae fed on leaves of maize and cassava was 74% and 60%, respectively. The larval period of the insects was shorter in maize 16.89 days (seven instars) and cassava 20.08 days (six instars). The pupal phase lasted 11.42 days in cassava treatment and 10.87 in the maize. The pupal weight of females and males was higher in corn 204.91 mg and 198.97 mg, respectively. The biological cycle varied depending on the ingested food. Adult longevity lasted 9.88 days for insects fed on cassava leaves. Therefore, cassava affected the development of S. frugiperda.

Aspectos biológicos e tabela de vida de fertilidade de Aphis gossypii Glover (Hemiptera: Aphididae) em cultivares de algodoeiro DeltaOPAL e NuOPAL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Root-supported overdentures associated with temporary immediate prostheses: a case-report

Complete denture fabrication can be a treatment challenge for many dentists, especially when the patient has remaining teeth. So, planning for use of clinical alternatives to improve the treatment prognosis and to enable greater comfort to patient must be considered. This case report describes the use of remaining roots to aid in the stability, support and retention of root-supported overdentures. Thus, immediate prostheses were planned for temporary rehabilitation prior to definitive overdentures. The aim of this study was to demonstrate the planning of root-supported overdentures attached with a ball system for retention associated with immediate dentures between the periods of preparation to fabrication of the definitive overdentures. The proposed treatment met the patient's needs such as being able to carry on normal activities during the sequencing of procedures to be performed over a period of time. The treatment had longevity with patient's knowledge about the necessity of oral hygiene and periodic controls.

First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)