Avaliação preliminar dos efeitos de Coffea arabica sobre a obesidade induzida por dieta hipercalórica

Obesity along with overweight, has been considered one of the most serious public health problems in the world, especially because they are the main risk factors for many chronic diseases, such as coronary heart disease, type II diabetes mellitus, hypertension and some types of tumors, which are associated with high mortality rates. The use of functional foods and appropriate diets to call health promotion has grown as a mechanism for prevention, control and treatment of chronic diseases such as obesity. Several ethnopharmacological studies indicate plant species for the treatment of disorders associated with obesity with a major attraction of this regimen is perceived as safer and more effective health than the traditional treatment with appetite suppressants. This work aims to develop and standardize models of biological assays in stress and obesity, also aims to evaluate the effect of oil green beans of Coffea arabica in the regulation of body weight and energy balance in mice. To this end, trials were made in vivo studies of this product and their effects on energy metabolism in non-obese mice with obesity induced by hypercaloric diet. After induction of obesity by 8 weeks, animals were treated for 21 days with the extracts orally. After 21 days the animals were killed to evaluate the effects of these products on daily feed intake and on body weight. The group treated with the oil of Coffea arabica L. showed significant weight loss and feed intake high. According to the results, we conclude that the standardized extract of Coffea arabica L. decreased body weight without restriction or decrease the amount of food ingested

Projeto de exploração de mercado e desenvolvimento de novos conceitos para segmento cosmético brasileiro: aplicação e avaliação de métodos de pesquisa

The current growth of the cosmetic Brazilian market and the incessant search for innovations requires the company´s Research & Development departments to be efficient and assertive. Therefore this work aimed to approach the process of development of new concepts, since the market exploration using quantitative tools, Survey, until the concept creation by qualitative tools. As results of this project an analysis of the tools and guidelines used nowadays and the creation of new exploration methods were delivered

Avaliação preliminar do efeito da Physalis angulata L. sobre a obesidade induzida por dieta hipercalórica

The obesity has been considered one of the most serious public health problem in the worldwide scale, especially for being one of the main risk factors for many chronic diseases that characterize the metabolic syndrome. In addition to these diseases, obesity also causes dermatoses that affect not only the individual emotions but also cause various cutaneous infections. Thus, overweighting can be an agent that causes many types of stress: psychological, physical and social. In order to improve people’s life quality, several ethnopharmacological studies indicate plant species for the treatment of disorders associated with obesity due to the attraction of this regimen is perceived as safer and more health effective when compared to the treatment performed by traditional anorectic substances. If considered both food intake and also oral treatment with different compounds can promote change in the gene expression, besides an appropriate diet has been maintenance mechanism of body's vital functions. The aim of this work was to develop new standard methods for induced obesity and stress, which stress is induced through physical and psychogenic stressors in Swiss male mice. Also to evaluate the effect of supercritical extract of Physalis angulata L. on the food intake and corporeal weight loss

Cultivo celular in vitro: importância para a pesquisa biomédica e dimensão da problemática de autenticação de linhagens celulares

Experimental models composed by human and animal cell lines are simplified and informative, allowing them to be widely used for biomedical research. Most laboratories that use in vitro cultivated cells maintain a variation of cell lines stored and cultivated. Therefore, misidentification and cross-contamination events can happen during cell lines handling. This problem can generate a repertoire of dubious results and papers, which may prejudice biomedical research. Recently it was created the International Cell Line Authentication Committee (ICLAC), which aims to spread knowledge about cross-contamination and misidentification of in vitro cell lines. Despite of the efforts spent trying to aware scientific community about the importance of the correct identification of cells, the number of papers based on misidentified cell lines it´s still worrying, compromising the reliability of out coming results and conclusions regarding them. The present study aims to analyze and discuss the main advantages and limitations of eukaryote in vitro cell lines use, characterizing the cell lines authentication problems. Therefore, compilation and critical analyses of literature data was realized, aiming to improve the understanding about this subject. Based on information about 445 cell lines with issues published by ICLAC it´s clear that contamination in human cell lines represented 89,2 % of mentioned problems. HeLa cell line was the responsible for most contamination, especially in 92 normal tissue cell lines, representing 44,6% of the contamination. These results reinforce the importance of periodic maintenance of cell lines cultures by labs and implementation of authentication methods as polymorphic STRs, besides obtaining cell lines from reliable sources and cell banks

Pesquisa e experiência: linhas de fuga e dramaturgia científica

The discussions waged by several authors in the field of philosophy of science about scientificity and scientific work often fall to the shock of Sciences, who had long substantiate their studies from the perspective of Natural Sciences. Fact that the man away from research, transforming it into a simple object of study. Among these questions, it is necessary to think in a very scientificity of the humanities, which considers the various types of knowledge that makes up the formation of the individual. In this way, approaching the experience, treated by Agambem Benjamin Larrosa and the educational research with children presents itself as a chance to rethink the current paradigm of Sciences. This gives rise to the objective of this work reflect, based on a research conducted with children and imagetic production, the idea of 'research and its relation to the concept of experience'. We will take as its starting point the elaborations on this concept as presented by Giorgio Agamben. We are assuming that the images of that research, are configured as research papers or research and, in this sense, the research is defined as a documentary research

Geologia e pesquisa mineral na região oeste do maciço Caritianas, municípios de Alto Paraíso e Itapuã D'Oeste, Rondônia

The granites Rondonianos, located in Rondônia Tin Pronvíncia, the southwestern portion of the Amazonian Craton, have great economic importance due to its large potential metallogenic. These granites stands out Intrusive Suite or Suite Granites Latest Rondônia Rondônia, with approximately 997-998 Ma (Bettencourt et al., 1997, 1999), for their tin mineralization having the largest reserves of this ore from Brazil. These tin mineralization in veins greissen are genetically related to these granites, deposits with different structural styles both within granitic plutons (endogreissen) as the host rocks (exogreissen). The western region of the Massif Caritianas has few published works, and the geology and potential econômicoão that were never fully known, now with the recovery of tin in the world market need new campaigns and prospective discoveries of new deposits

Pesquisa-ação sobre o uso de indicadores de desempenho do equipamento em uma fábrica de geradores

In the industrial environment the challenge is use better the productive resources: people and machine. The following work has the main goal improve the efficient losses analysis in the stator bar’s production bottleneck equipment situated in the Electric generator’s factory. The action research involved Theory of Constraints on the restriction system identification and developed the data collection framework by losses typology for indicator measurement. The research showed the data collection standardization importance to obtain reliable data and strategic efficiency indicator to optimize equipments. Besides of this, OEE and TEEP indicator demonstrated efficiency results to analyze the actual efficiency when the machine works and the increase capacity opportunity to treat the hide costs in the organization following the continuous improvement

Caracterização morfológica e fenotípica de leveduras do gênero Cryptococcus com ênfase na pesquisa de Cryptococcus neoormans isoladas de amostras de água de mar e areia de três ambientes costeiros do estado de São Paulo: Canal de São Sebastião, Baixada Santista e Ubatuba

The yeast Cryptococcus neoformans is the etiologic agent of cryptococcosis, an infectious cosmopolitan disease that affects humans. Although rare, this disease is potentially fatal, especially for immunocompromised hosts. This pathogen is frequently isolated from excrements of pigeons and parrots, with many environmental sources such as birds, pigeon droppings, eucalyptus leaves, decaying trees, towers, churches and places of storage of grain (the port area). The isolation of this microorganism has been obtained also from the aquatic environment. The identification of environmental sources is needed to protect human health, especially susceptible populations such as immunocompromised. Therefore, this study investigated the presence of Cryptococcus neoformans in yeast isolates obtained from samples of sea water and sand from three regions of São Paulo: São Sebastião Channel, Santos and Ubatuba. Isolates were analyzed according to micro-and macroscopic characteristics and biochemical tests: microculture, urease, ink nankin, auxanograma, zymogram and phenol. We analyzed 199 isolates, 175 of which had features suggestive for Cryptococcus spp. in microculture. All these 175 isolates were sown in the Christensen urea middle to verify the production of urease and submitted to the technique nankin ink to visualize the capsule. Of these, only 24 were selected for the next test that was the auxanograma (assimilation of carbohydrate and nitrogen). Of the 24, 10 were tested in zymograms (fermented sugar), from which 5 were selected for the phenoloxidase test in medium containing dopamine. None of the 5 isolates tested had black or brown color characteristic of Cryptococcus neoformans. According to these tests, we arrived at 5 isolates identified to the genus Cryptococcus, but not the neoformans specie

Avaliação preliminar dos efeitos de Bidens pilosa L. sobre a obesidade induzida por dieta hipercalórica

The obesity along with overweight has been considered one of the most serious public health problems in the world, especially because they are the main risk factors for many chronic diseases such as coronary heart disease, diabetes mellitus, hypertension and some types of tumors, which are associated with high mortality rates. The use of functional foods and appropriate diets to promote health has grown as a prevention mechanism. Previous studies suggested Bidens pilosa L. for the treatment of disorders associated with obesity, with one important attraction of this regiment is perceived as safer and more effective health than the traditional anorexigen .The aim of this project is to standardize a new model of obesity induced by hipercaloric diet in Swiss mice, and also to evaluate the effect of Bidens pilosa L. extract in the reduction of food intake, weight and toxicity. After induction of obesity, the animals were treated for 21 days with the extract. Then the animals were killed and samples were collected for further analysis. Preliminary, the effect of this product has being evaluated on the daily food intake and body weight. In dose of 100mg/Kg, there were no significant changes in food intake and body weight. Thus, this concentration of Bidens pilosa was not effective in reducing body weight of obese animals, and did not reduce food intake

Aumento da precisão do controle de estoque: pesquisa ação em indústria de bens de consumo

This work is an action research conducted in an industry of consumer goods, presenting a new model of inventory management in the company's processors. This replacement of the inventory management of the company stemmed from the need to reduce the large number of deviations in the product write off in stock, thereby generating a low accuracy and reliability of data on inventories of processors shown by the company's ERP system. Spending on inventory adjustments could thus be reduced with the implementation of the new model, thus generating a cost savings for the company and thus increasing their competitive potential in the market. In the old system adopted by the company, write off raw material inventory was done automatically by the system for customized transactions by the company. However, since the implementation of ERP in the company, the automatic write off based on historical consumption of each product were made in many cases at random, generating a lot of mistakes. The new management system has replaced the automatic system by manual at the time of the return of the processed product in the company, thus creating a control which lots and quantities were consumed in the processing and making the stock shown by the ERP reliable and accurate

Pesquisa de genes de resistência do M. tuberculosis a isoniazida e rifampicina por sequenciamento e pela tecnologia de DNA-STRIP

Tuberculose (TB), causada por Mycobacterium tuberculosis, é uma das doenças infecciosas que mais causam mortes. Estima-se que mais de dois bilhões de pessoas estejam infectadas no mundo. O tratamento da TB consite em associação de fármacos, isoniazida, rifampicina, pirazinamida e etambutol, nos primeiros 2 meses e 4 meses de isoniazida e de rifampicina. Internacionalmente, são consideradas cepas multi resistentes (MDR), as que apresentam resistência simultânea a isoniazida e a rifampicina. A rápida detecção de resistência é essencial para o controle e tratamento da TB, reduzindo, assim, o custo do tratamento e a transmissão da doença. Neste projeto, os isolados já identificados fenotipicamente como resistentes a isoniazida e/ou rifampicina, foram submetidos ao sequenciamento de Sanger para pesquisa de 3 genes relacionados a resistência a isoniazida (katG, inhA e ahpC) e 1 gene de resistência a rifampicina (rpoB). Foi realizada uma comparação destes genes mutados para a resistência utilizando o novo teste desenvolvido pela Biomérieux, denominado GenoType® MTBDRplus, que se baseia na tecnologia DNA-STRIP. Através deste novo teste, foi observada mutação em 22 isolados clínicos de M. tuberculosis para genes de resistência a isoniazida e/ou rifampicina, sendo 4 provenientes do MS e 18 de SP. Já pelo sequenciamento genético foi observada mutação em 24 isolados para genes de resistência a isoniazida e/ou rifampicina, sendo 6 provenientes do MS e 18 de SP. Portanto, através do sequenciamento de Sanger, foi possível detectar um número maior de isolados mutados e mais mutações quando comparado ao teste GenoType® MTBDRplus. Isso acontece porque na técnica de sequenciamento, um fragmento do gene como um todo é analisado e no caso do teste GenoType® MTBDRplus, é verificada apenas a ausência ou presença das mutações mais frequentes descritas na literatura, além de não ser analisado o gene ahpC. A grande ...

A formação do professor reflexivo no periódico Educação e Pesquisa - 1984 a 2010

Este estudo teve como objetivo compreender a formação docente na perspectiva do professor reflexivo, buscando averiguar nesse processo temas emergentes vinculados ao periódico Educação e Pesquisa, fonte de coleta de dados. Neste itinerário optamos pela pesquisa qualitativa, do tipo descritivo, escolhendo como técnica para a coleta de dados a fonte documental, seguida de análise documental. Entre os resultados coletados na revista, periódico de 1975 a 2010, apontaram-se como significativos os eixos reflexão, saberes, identidade e profissionalização docente, prática pedagógica, formação inicial e formação continuada. Neste contexto observou-se que as pesquisas de ênfase critico reflexiva vem contribuindo teoricamente para a disseminação do conceito da formação reflexiva inicial e continuada de professores que buscam por meio desta perspectiva o desenvolvimento profissional e o reconhecimento como produtores de conhecimento na área que atuam. Outras questões observadas assinalaram para a compreensão da formação reflexiva como uma concepção que pode auxiliar o docente no processo de reconhecimento da sua identidade profissional marcada pela influência direta da cultura da qual os docentes estão inseridos. Nesta direção foi também identificado que as tomadas de decisões no âmbito escolar, dentro e fora da sala de aula, requerem do docente a mobilização de saberes que vão além da teoria aprendida nos anos de formação acadêmica, buscando auxilio nas vivências pré-profissionais para a elaboração dos saberes docentes. Porém, elucidou-se que os docentes reflexivos e investigadores da sua própria prática tendem a ser favorecidos por este tipo de prática, podendo provocar inovações na educação e em seu próprio desenvolvimento profissional. Concluindo, pode-se dizer que o estudo do periódico Educação e Pesquisa perspectivado... (Resumo completo, clicar acesso eletrônico abaixo)

Regiões competitivas agrícolas e vulnerabilidade territorial: o papel do Consórcio Pesquisa Café

This academic work presents the final results of the undergraduate research about new agricultural technical systems developed by “Consórcio Pesquisa Café” (CPC), which main objective was to analyze the impacts of scientific inputs in different coffee producing regions, particularly in South of Minas Gerais. The creation of the CPC meets the demands of a global scientific coffee-growing (SANTOS, 2000) under development in the current period technical-scientific-informational (SANTOS, 2009). On this period, also called globalization, the technoscience, the information and the finances guide the actions of companies and institutions submitted by imperative of competitiveness on international market. The Brazilian’s coffee-growing regions (South of Minas Gerais, Cerrado Mineiro, Espírito Santos Ridge’s, Western Bahia, among others) on search of higher productivities, begin gather technical, informational and communicational densities (SANTOS, 2009). Considering these facts, we detail in this research, the major agricultural technical systems developed by “Consórcio Pesquisa Café” (CPC), expressed in new management techniques, new cultivars and new chemicals, mechanical and biological inputs. In the concluding part of this undergraduate research we analyze how these new technical systems contribute to increased competitiveness and vulnerability of South of Minas Gerais in front of international market

Treinamento aeróbio na prevenção de alterações cardíacas decorrentes da obesidade em ratos (Rattus Norvegicus Albinus, Wistar)

A obesidade é um problema de saúde pública mundial e está fortemente associada à doença cardíaca isquêmica, aterosclerose, hipertensão arterial, entre outros problemas cardiovasculares. Na atualidade, o tratamento sugerido para as doenças cardiovasculares, comumente decorrentes da obesidade, consiste na diminuição da ingestão de gordura aliada à atividade física. Contudo, pouco se sabe quanto aos protocolos de treinamento mais adequados para a prevenção ou tratamento dessas doenças. O presente estudo objetivou analisar os efeitos do exercício físico na intensidade de 80% do limiar anaeróbio (LAn) sobre os aspectos bioquímicos cardíacos de ratos obesos. Para a indução da obesidade, os animais, a partir dos 90 dias de idade, foram mantidos com dieta hiperlipídica/hipercalórica (35% de gordura animal/vegetal) e os animais controles, mantidos com dieta balanceada (AIN-93M). Todos os animais pertencentes ao grupo treinado foram submetidos a testes de lactato mínimo para a identificação do limiar anaeróbio (LAn) durante exercício de natação, treinando desde os 28 dias de idade até aos 90 dias em intensidade equivalente a 80% do LAn 1 h/dia, 5 dias/semana. Depois dos 90 dias uma parte dos animais treinados interrompeu o treinamento ficando em observação até o final do experimento (150 dias de idade). Os demais animais permaneceram sedentários ao longo de todo o experimento. Foram analisados: peso corporal, tolerância à glicose e à insulina (TTGo e TTI), perfil lipídico sérico (colesterol total, triglicerídeos, AGL e HDL), concentrações séricas de glicose e insulina e alíquotas do ventrículo esquerdo foram retiradas para análise das concentrações de triglicerídeos, glicogênio, proteína/DNA e atividade lipogênica. Os animais tratados com dieta hiperlipídica tiveram aumento significativo no peso corporal, intolerância à glicose e resistência à insulina e o treinamento físico neutralizou tais...

Horto de plantas medicinais e tóxicas “Prof.ª Dra. Célia Cebrian Araújo Reis”: centro de referência botânica para o ensino, a pesquisa e a extensão

O Horto de Plantas Medicinais e Tóxicas “Prof.ª Dra. Célia Cebrian Araújo Reis” fundado em 1989 no campus de Araraquara da UNESP é um Horto de Referência Botânica e uma coleção de plantas vivas de interesse farmacêutico, objeto deste estudo que objetivou em caracterizá-lo e divulgá-lo. Para isso foi realizado um resgate histórico por meio de pesquisas em documentos datados da época da fundação e realização de entrevistas com um dos docentes fundadores e com o funcionário da unidade; levantamento florístico com seleção de algumas espécies arbóreas para depósito em herbário e registro, assim como o desenvolvimento de um sistema heurístico de catalogação própria para dar entrada nas plantas que futuramente virão a ser registradas no horto, modelo de ficha monográfica botânica e exemplo de ficha devidamente preenchida; e a construção de página na internet vinculada ao site da FCFAr disponibilizada na Web apresentando parte do conteúdo desenvolvido neste trabalho, incluindo lista de plantas registradas para consulta.