965 resultados para Mt. Everest


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本文报道了利用乘积算符方法分析多脉冲及二维FT-NMR实验的模拟程序PROPER-MT.该程序对分析弱耦合I_mS_n(I=1/2;S=1/2;1≤m十n<4)自旋体系实验脉冲序列是普遍适用的;它可给出实验过程中体系任何时刻算符的解析表达式.用PROPER-MT序对一些典型的多脉冲及二维FT-NMR实验进行了模拟,特别对多量子滤波及多自旋滤波脉冲序列进行了分析计算,得到了预期的结果.

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To understand the systematic status of Larimichthys crocea in the Percoidei, we determined the complete mitochondrial (mt) genome sequence using 454 sequencing-by-synthesis technology. The complete mt genome is 16,466 bp in length including the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and the noncoding control region (CR). Further sequencing for the complete CR was performed using the primers Cyt b-F and 12S-R on six L crocea individuals and two L polyactis individuals. Interestingly, all seven CR sequences from L crocea were identical while the three sequences from L polyactis were distinct (including one from GenBank). Although the conserved blocks such as TAS and CSB-1, -2, and -3 are readily identifiable in the control regions of the two species, the typical central conserved blocks CSB-D, -E, and -F could not be detected, while they are found in Cynoscion acoupa of Sciaenidae and other Percoidei species. Phylogenetic analysis shows that L crocea is a relatively recently emerged species in Sciaenidae and this family is closely related to family Pomacanthidae within the Percoidei. L crocea, as the first species of Sciaenidae with complete mitochondrial genome available, will provide important information on the molecular evolution of the group. Moreover, the genus-specific pair of primers designed in this study for amplifying the complete mt control region will be very useful in studies on the population genetics and conservation biology of Larimichthys. (c) 2008 Elsevier B.V. All rights reserved.

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Metallothionein (MT) is a superfamily of cysteine-rich proteins contributing to metal metabolism, detoxification of heavy metals, and immune response such as protecting against ionizing radiation and antioxidant defense. A metallothionein (designated AiMT2) gene was identified and cloned from bay scallop, Argopecten irradians. The full length cDNA of AiMT2 consisted of an open reading frame (ORF) of 333 bp encoding a protein of 110 amino acids. with nine characteristic Cys-X-Cys, five Cys-X-X-Cys, five Cys-X-X-X-Cys and two Cys-Cys motif arrangements and a conserved structural pattern Cys-x-Cys-x(3)-Cys-Tyr-x(3)Cys-x-Cys-x(3)-Cys-x-Cys-Arg at the C-terminus. The cloned ANT showed about 50% identity in the deduced amino acid sequence with previously published MT sequences of mussels and oysters. The conserved structural pattern and the close phylogenetic relationship of AiMT2 shared with MTs from other mollusc especially bivalves indicated that AiMT2 was a new member of molluscan MT family. The mRNA transcripts in hemolymph of AiMT2 under cadmium (Cd) exposure and bacteria challenge were examined by real-time RT-PCR. The mRNA expression of AiMT2 was up-regulated to 3.99-fold at 2 h after Listonella anguillarum challenge, and increased drastically to 66.12-fold and 126.96-fold at 16 and 32 h post-challenge respectively. Cadmium ion exposure could induce the expression of AiMT2, and the expression level increased 2.56-fold and 6.91-fold in hemolymph respectively after a 10-day exposure of 100 mu g L-1 and 200 mu g L-1 CdCl2. The sensitivity of AiMT2 to bacteria challenge and cadmium stress indicated it was a new Cd-dependent MT in bay scallop and also regulated by an immune challenge. The changes in the expression of AiMT2 could be used as an indicator of exposure to metals in pollution monitoring programs and oxidative stress, and bay scallop as a potential sentinel organism for the cadmium contamination in aquatic environment. (C) 2008 Elsevier Inc. All rights reserved.

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A method for culturing medulla terminalis (MT) neurons in the eyestalk of Chinese shrimp, Fenneropenaeus chinensis, was first established. The neurons showed immediate outgrowth in the culture medium supplemented with glutamine, glucose and antibiotics. The cells grew for about 2-7 days and then sustained for a week or more. At least six types of neurons were distinguished on the basis of size and form of soma and outgrowth pattern of cells. (C) 2003 Elsevier Science B.V. All rights reserved.

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The complete mitochondrial (mt) genome sequence of Oratosquilla oratoria (Crustacea: Malacostraca: Stomatopoda) was determined; a circular molecule of 15,783 bp in length. The gene content and arrangement are consistent with the pancrustacean ground pattern. The mt control region of O. oratoria is characterized by no GA-block near the 3' end and different position of [TA(A)]n-blocks compared with other reported Stomatopoda species. The sequence of the second hairpin structure is relative conserved which suggests this region may be a synapomorphic character for the Stomatopoda. In addition, a relative large intergenic spacer (101 bp) with higher A + T content than that in control region was identified between the tRNA(Glu) and tRNA(Phe) genes. Phylogenetic analyses based on the current dataset of complete mt genomes strongly support the Stomatopoda is closely related to Euphausiacea. They in turn cluster with Penaeoidea and Caridea clades while other decapods form a separate group, which rejects the monophyly of Decapoda. This challenges the suitability of Stomatopoda as an outgroup of Decapoda in phylogenetic analyses. The basal position of Stomatopoda within Eumalacostraca according to the morphological characters is also questioned. (C) 2010 Elsevier Inc. All rights reserved.

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The annual cycle of nutrient-phytoplankton dynamics in Bohai Sea (BS) is simulated using a coupled physical-biological model in this study. By comparison, the modeled seasonal variations of nutrients and primary productivity agree with observations rather well. Although the annual cycles of chlorophyll a and primary production are both characterized by a double-peak configuration, a structural difference is still apparent: the phytoplankton biomass reaches the highest value in spring while summer is characterized by the most productivity in the BS, which can be ascribed to the combined impact of seawater temperature and zooplankton-grazing pressure on the growth of algae. Based on the validated simulations, the annual budgets of carbon, nitrogen and phosphorus are estimated, and are about 0.82 mt C surplus, 39 kt N deficit and 12kt P surplus, respectively, implying that the BS ecosystem is somewhat nitrogen limited. The contribution of two external nutrient sources, namely river discharges and resuspended sediments, to the growth of algae is also examined numerically, and it is found that the influence of river-borne nutrients mainly concentrates in estuaries, whereas the reduction of sediment-borne nutrients may significantly inhibit the onset of algae bloom in the whole BS. (c) 2007 Elsevier Ltd. All rights reserved.

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本论文基于对我国沿海重金属污染加剧以及滩涂贝类资源衰退现象的关注,围绕典型滩涂贝类四角蛤蜊生物化学、细胞生物学和免疫学特征开展研究,调查了渤海湾天津近岸海域四角蛤蜊体内重金属含量和分布规律,以及四角蛤蜊对沉积物重金属的富集能力。室内模拟研究了四角蛤蜊对重金属镉和汞的富集能力及同化机制(assimilation mechanism)差异性,四角蛤蜊各组织对镉和汞的解毒机理和负载能力,以及重金属胁迫对四角蛤蜊血细胞结构和功能的损伤效应。研究结果可望为探讨四角蛤蜊受重金属胁迫细胞水平上的响应机制,揭示重金属污染和贝类资源衰退的关系,建立快速可靠的重金属污染生物标志物指标体系,开展生态健康评估和生境修复等提供科学依据。主要研究结果如下: 1.查明了渤海湾天津近岸四角蛤蜊体内和表层沉积物中Cd、Pb、Cu、Zn、Mn、Cr和Ni七种重金属的含量和周年时空分布特征;表层沉积物中Cd具有较强的污染程度和潜在生态风险,四角蛤蜊对重金属元素Cd和Pb具有较高的富集能力,对环境重金属污染具有很好的指示作用。 2.查明了Cd对四角蛤蜊在24h,48h和96h的半致死浓度(LC50)分别为15.96mg/L、5.15mg/L和2.38mg/L,汞的24h,48h和96h LC50分别为3.71mg/L,0.61mg/L和0.21mg/L。镉和汞对四角蛤蜊的安全质量浓度分别为0.0238mg/L和0.0021mg/L。 3.四角蛤蜊软体部对Cd和Hg的富集能力有显著差异。暴露过程中,四角蛤蜊软体部Cd和Hg的增加量分别为0.12-7.7µg/g和0.002-0.024µg/g,富集率分别为0.3-6.2%和0.11-0.68%,吸收率常数分别为0.07-1.10和0.001-0.005。 4.四角蛤蜊受Cd和Hg胁迫后,外套膜、鳃和肝胰腺的金属硫蛋白(MT含量均在暴露浓度和胁迫时间上都有极显著变化。组织内MT量的大小关系为:肝胰腺>鳃>外套膜。肝胰腺可以作为双壳贝类MT金属污染指示研究的目标组织。四角蛤蜊对Cd和Hg不同的解毒机制,导致组织中MT表达含量和Cd胁迫显著相关,而与Hg胁迫无显著相关性。 5.四角蛤蜊肝胰腺和鳃中抗氧化系统酶、脂质过氧化产物、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)在不同Cd胁迫浓度和暴露时间下有显著差异。与对照组相比,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)的活力和丙二醛(MDA)含量均随着暴露时间的增加而增加,达到一个峰值,然后减低。而ACP和ALP活力则表现出先被抑制,然后升高的变化趋势。肝胰腺中CAT和GPx活力高于鳃,鳃中SOD和MDA含量高于肝胰腺,这种差异与两种组织不同的解毒机理有关。 6.重金属胁迫能够造成四角蛤蜊血细胞超微结构的损伤、溶酶体膜稳定性改变、微核和总畸形核生成,各种损伤均表现出明显的浓度依赖效应。2μg/L Hg暴露14天后,血细胞溶酶体膜中性红保持时间(NRR)、微核生成率(MN)和总畸形核生成率(TNA)与对照组相比没有显著差异。相同暴露时间下,25 μg/L Hg处理组血细胞NRR值低于25 μg/L Cd处理组,而MN和TNA值则相反,说明Hg胁迫对四角蛤蜊血细胞溶酶体膜具有较强的损伤作用。研究发现,NRR、MN和TNA三种指标对于衡量重金属污染对四角蛤蜊血细胞的毒性效应有很好的协同检测作用,可望作为有效的生物标志物在环境监测技术中得以应用。

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大地电磁(MT方法是一种新发展的地球物理技术,近十年已被国外越来越多的研究者用于海底电磁场的调查,旨在获得海底的电性结构。基于自1990年3月10至1990年3月22日在中日全用KX90-1航次以一海底电位磁力计(OBEM)于31°52.47'N, 129°7.17'E,水深884米处所取得的电磁场资料,通过各种数字信号处理,得到一维大地电磁的测深结果,首次确定了冲绳海槽北部的电性结构如下:层位(向下) 电阻率(欧姆·米) 厚度(千米) 1 0.3 3 2 5000 20 3 0.6 4 4 5000 13 5 100 100 基底 2 ∞,根据已知的冲绳海槽及周转地区的地质地球物理资料,对于所研究地区从以上的电性结构可以得到以下几点主要认识:1、莫霍面埋深或地壳厚度约为27千料;2、岩石圈厚度约40千米;3、软流圈厚度约100千米;4、壳内高导层是由于岩石的熔融或部分熔融;5、热流值大致在60-100毫瓦/米~2。

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利用ERA40逐日再分析资料、NCEP/NCAR2逐日再分析资料、中国740个测站日降水资料、上海台风研究所提供的西太平洋热带气旋资料、Kaplan等重建的月平均SSTA资料、NOAA逐日长波辐射(OLR)等资料,应用离散功率谱分析、带通滤波、EOF分析等统计方法,研究了东亚夏季风(EASM)的移动特征、东亚地区季节内振荡(ISO)的基本特征、季节内振荡对东亚夏季风活动的影响、季节内振荡对东亚夏季风异常活动的影响机理。主要结论如下: (1)综合动力和热力因素定义了可动态描述东亚夏季风移动和强度的指数,并利用该指数研究了东亚夏季风的爆发和移动的季节内变化及其年际和年代际变化特征。研究发现,气候平均东亚夏季风前沿分别在28候、33候、36候、38候、40候、44候出现了明显的跳跃。东亚夏季风活动具有显著的年际变率,主要由于季风前沿在某些区域异常停滞和突然跨越北跳或南撤引起,造成中国东部旱涝灾害频繁发生。东亚夏季风的活动具有明显的年代际变化,在1965年、1980年、1994年发生了突变,造成中国东部降水由“南旱北涝”向“南涝北旱”的转变。 (2)东亚季风区季节内变化具有10~25d和30~60d两个波段的季节内振荡周期,以30-60d为主。存在三个主要低频模态,第一模态主要表征了EASM在长江中下游和华北地区活动期间的低频形势;第二模态印度洋-菲律宾由低频气旋式环流控制,主要表现了ISO在EASM爆发期间的低频形势;第三模态主要出现在EASM在华南和淮河活动期间的低频形势。第一模态和第三模态是代表东亚夏季风活动异常的主要低频形势。 (3)热带和副热带地区ISO总是沿垂直切变风的垂直方向传播。因此,在南海-菲律宾东北风垂直切变和副热带西太平洋北风垂直切变下,大气热源激发菲律宾附近交替出现的低频气旋和低频反气旋不断向西北传播,副热带西太平洋ISO以向西传播为主。中高纬度地区,乌拉尔山附近ISO以向东、向南移动或局地振荡为主;北太平洋中部ISO在某些情况下向南、向西传播。 (4)季风爆发期,伴随着热带东印度洋到菲律宾一系列低频气旋和低频反气旋, 冷空气向南输送,10~25天和30~60天季节内振荡低频气旋同时传入南海加快了南海夏季风的爆发。在气候态下,ISO活动表现的欧亚- 太平洋(EAP)以及太平洋-北美(PNA)低频波列分布特征(本文提出的EAP和PNA低频波列与传统意义上的二维定点相关得到的波列不同)。这种低频分布形式使得欧亚和太平洋中高纬度的槽、脊及太平洋副热带高压稳定、加强,东亚地区的低频波列则成为热带和中高纬度ISO相互作用影响东亚夏季风活动的纽带。不同的阶段表现不同的低频模态,30~60d低频模态的转变加快了EASM推进过程中跳跃性;30-60d低频模态的维持使得EASM前沿相对停滞。 (5)30-60d滤波场,菲律宾海域交替出现的低频气旋和低频反气旋不断向西北传播到南海-西太平洋一带。当南海-西太平洋地区低频气旋活跃时,季风槽加强、东伸,季风槽内热带气旋(TC)频数增加;当南海-西太平洋低频反气旋活跃时,季风槽减弱、西退,TC处于间歇期,生成位置不集中。 (6)在El Nino态下,大气季节内振荡偏弱,北传特征不明显,但ISO由中高纬度北太平洋中部向南和副热带西太平洋向西的传播特征显著,东亚地区ISO活动以第三模态为主,EASM集中停滞在华南和淮河流域,常伴随着持续性区域暴雨的出现,易造成华南和江淮流域洪涝灾害,长江和华北持续干旱。在La Nina态下,大气季节内振荡活跃,且具有明显的向北传播特征,PNA低频波列显著,东亚地区ISO活动以第一模态单峰为主;EASM主要停滞在长江中下游和华北地区,这些地区出现异常持续强降水,华南和淮河流域多干旱;在El Nino态向La Nina态转换期,ISO活动以第一模态双峰为主,长江中下游常常出现二度梅。

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Duplications and rearrangements of coding genes are major themes in the evolution of mitochondrial genomes, bearing important consequences in the function of mitochondria and the fitness of organisms. Yu et al. (BMC Genomics 2008, 9: 477) reported the complete mt genome sequence of the oyster Crassostrea hongkongensis (16,475 bp) and found that a DNA segment containing four tRNA genes (trnK(1), trnC, trnQ(1) and trnN), a duplicated (rrnS) and a split rRNA gene (rrnL5') was absent compared with that of two other Crassostrea species. It was suggested that the absence was a novel case of "tandem duplication-random loss" with evolutionary significance. We independently sequenced the complete mt genome of three C. hongkongensis individuals, all of which were 18,622 bp and contained the segment that was missing in Yu et al.'s sequence. Further, we designed primers, verified sequences and demonstrated that the sequence loss in Yu et al.'s study was an artifact caused by placing primers in a duplicated region. The duplication and split of ribosomal RNA genes are unique for Crassostrea oysters and not lost in C. hongkongensis. Our study highlights the need for caution when amplifying and sequencing through duplicated regions of the genome.

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Background: There are many advantages to the application of complete mitochondrial (mt) genomes in the accurate reconstruction of phylogenetic relationships in Metazoa. Although over one thousand metazoan genomes have been sequenced, the taxonomic sampling is highly biased, left with many phyla without a single representative of complete mitochondrial genome. Sipuncula (peanut worms or star worms) is a small taxon of worm-like marine organisms with an uncertain phylogenetic position. In this report, we present the mitochondrial genome sequence of Phascolosoma esculenta, the first complete mitochondrial genome of the phylum. Results: The mitochondrial genome of P. esculenta is 15,494 bp in length. The coding strand consists of 32.1% A, 21.5% C, 13.0% G, and 33.4% T bases (AT = 65.5%; AT skew = -0.019; GC skew = -0.248). It contains thirteen protein-coding genes (PCGs) with 3,709 codons in total, twenty-two transfer RNA genes, two ribosomal RNA genes and a non-coding AT-rich region (AT = 74.2%). All of the 37 identified genes are transcribed from the same DNA strand. Compared with the typical set of metazoan mt genomes, sipunculid lacks trnR but has an additional trnM. Maximum Likelihood and Bayesian analyses of the protein sequences show that Myzostomida, Sipuncula and Annelida (including echiurans and pogonophorans) form a monophyletic group, which supports a closer relationship between Sipuncula and Annelida than with Mollusca, Brachiopoda, and some other lophotrochozoan groups. Conclusion: This is the first report of a complete mitochondrial genome as a representative within the phylum Sipuncula. It shares many more similar features with the four known annelid and one echiuran mtDNAs. Firstly, sipunculans and annelids share quite similar gene order in the mitochondrial genome, with all 37 genes located on the same strand; secondly, phylogenetic analyses based on the concatenated protein sequences also strongly support the sipunculan + annelid clade (including echiurans and pogonophorans). Hence annelid "key-characters" including segmentation may be more labile than previously assumed.

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The complete mitochondrial (mt) DNA sequence was determined for a ridgetail white prawn, Exopalaemon carinicauda Holthuis, 1950 (Crustacea: Decopoda: Palaemonidae). The mt genome is 15,730 bp in length, encoding a standard set of 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which is typical for metazoans. The majority-strand consists of 33.6% A, 23.0% C, 13.4% G, and 30.0% T bases (AT skew = 0.057: GC skew = -0.264). A total of 1045 bp of non-coding nucleotides were observed in 16 intergenic regions,,including a major A+ T rich (79.7%) noncoding region (886 bp). A novel translocation of tRNA(Pro) and tRNA(Thr) was found when comparing this genome with the pancrustacean ground pattern indicating that gene order is not conserved among caridean mitochondria. Furthermore, the rate of Ka/Ks in 13 protein-coding genes between three caridean species is Much less than 1, which indicates a strong Purifying selection within this group. To investigate the phylogenetic relationship within Malacostraca, phylogenetic trees based oil Currently available malacostracan complete mitochondrial sequences were built with the maximum likelihood and Bayesian models. All analyses based oil nucleotide and amino acid data strongly support the monophyly of Decapoda. The Penaeidae, Reptantia, Caridea, and Meiura clades were also recovered as monophyletic groups with Strong Statistical Support. However, the phylogenetic relationships within Pleocyemata are unstable, as represented by the inclusion or exclusion of Caridea. (C) 2009 Elsevier B.V. All rights reserved.

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There are 47 genera and 161 species of Gramineae except the cultivated species in the area of the Karakorum and Kunlun Mountains. The results of research on the distribution of the genera and species of Gramineae in the Karakorum and Kunlun Mountains show that (1) The Gramineae mainly contains elements of North Temperate, rich Old Word Temperate and other Temperate. It is obvious that the floristic nature of Gramineae in the Karakorum and Kunlun Mountains is the North Temperate; (2) All Pantropic genera can stretch to the Temperate Zone in this region, which all parts of the Pantropic type are the Temperate nature to a certain degree. For example, Erianthus ravennae from mediterranean to the Karakorum and Kunlun Mountains through the Central Asia; (3) As most genera of Grasses are the type of Temperate and the Frigid Zone, they have distinct floristic characteristics of mountainous and plateau flora such as Orinus, Alopecurus, Elymus, Trisetum, Littledalea, Elytrigia, Stephanachne and Paracolpodium etc. All of these indicate adaptive phenomenon of alpine specialization and cold-xerophilization on Grasses in this area; (4) Endemic genus of Gramineae is absent due to its nature and history and the endemic species are also rare in the Karakorum and Kunlun Mountains. Most of the genera with one or fewer species have originated from its relative and widespread genera, such as Ptilagrostis from Stipa, Timouria from Achnatherum, and so on; (5) Flora of the Karakorum and Kunlun Mountains is most closely related to the flora of Tibet, and is also extensively to its adjacent areas.

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In the world-wide zoogeographic division, there has been no consentaneous understanding about the delimitation between palaearctic and oriental realms in western China. In this study, we will discuss the division based on amphibian distribution in Shaanxi, Gansu, Sichuan, Yunnan, and Tibet according to species coefficient similarity between each zoogeographic province. The results show that the northern border lies from Qinling Mountains-Feng Xian (Shaanxi)-Debu (Gansu)-Aba (Sichuan)-Batang-Bomi (Tibet), to Linzhi districts, and the southern border is from Taibai-Feng Xian in Shaanxi-Wen Xian (Gansu)-Songpan-Kangding-Daocheng (Sichuan), to Zhongdian-Gongshan in Yunnan, and westward to Motuo and Bomi district in Tibet. (c) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.

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As a complement to conventional MT, Long-period Magnetotellurics (LMT) has been developed at low frequency for soundings of deep electric structures. Eastern Himalayan Syntaxis (EHS) and surrounding area is a key place for the study of dynamics of the uplift of Tibetan plateau. Experiments in the pioneer studies for EHS3D project showed that the study area shares an unusual low resistive crust and upper mantle. Conventional MT could not provide sufficient information about the deep structures of the study area that requested long period MT measurement to be complemented. This thesis presents the LMT studies in eastern Tibet along the EHS3D-3 Profile from Xiachayu to Yushu including data acquisition, processing, inversion and interpretation. The effective period of the measured LMT signals extend from 10s up to 30000s for the duration more than one week measurement. The resulting model shows that the LMT sounding coincides with the MT data in overlapped periods. Especially the induction arrows and tippers derived from LMT data provide more information about the base of the conductors beneath the plateau with higher resolution. Anomalous induction coefficients and 2-D model suggest extensive conductive bodies beneath Lhasa block and Qiangtang terrain which would be a possible evidence for partial melt and fluids at depth.