Propuesta de plan de manejo de San Miguel de los Baños

Estudi de la localitat de San Miguel de los Baños (Cuba) segons el punt de vista de l’ecoturisme per tal de proposar la zona com a àrea protegida i evitar la seva degradació

Molecular and immunological characterization of gluten proteins isolated from oat cultivars that differ in toxicity for celiac disease.

A strict gluten-free diet (GFD) is the only currently available therapeutic treatment for patients with celiac disease (CD). Traditionally, treatment with a GFD has excluded wheat, barley and rye, while the presence of oats is a subject of debate. The most-recent research indicates that some cultivars of oats can be a safe part of a GFD. In order to elucidate the toxicity of the prolamins from oat varieties with low, medium, and high CD toxicity, the avenin genes of these varieties were cloned and sequenced, and their expression quantified throughout the grain development. At the protein level, we have accomplished an exhaustive characterization and quantification of avenins by RP-HPLC and an analysis of immunogenicity of peptides present in prolamins of different oat cultivars. Avenin sequences were classified into three different groups, which have homology with S-rich prolamins of Triticeae. Avenin proteins presented a lower proline content than that of wheat gliadin; this may contribute to the low toxicity shown by oat avenins. The expression of avenin genes throughout the development stages has shown a pattern similar to that of prolamins of wheat and barley. RP-HPLC chromatograms showed protein peaks in the alcohol-soluble and reduced-soluble fractions. Therefore, oat grains had both monomeric and polymeric avenins, termed in this paper gliadin- and glutenin-like avenins. We found a direct correlation between the immunogenicity of the different oat varieties and the presence of the specific peptides with a higher/lower potential immunotoxicity. The specific peptides from the oat variety with the highest toxicity have shown a higher potential immunotoxicity. These results suggest that there is wide range of variation of potential immunotoxicity of oat cultivars that could be due to differences in the degree of immunogenicity in their sequences.

R-ESHAP as salvage therapy for patients with relapsed or refractory diffuse large B-cell lymphoma: the influence of prior exposure to rituximab on outcome. A GEL/TAMO study.

BACKGROUND The role of re-treatment with rituximab in aggressive B-cell lymphomas still needs to be defined. This study evaluated the influence of prior exposure to rituximab on response rates and survival in patients with diffuse large B-cell lymphoma treated with rituximab plus etoposide, cytarabine, cisplatinum and methylprednisolone (R-ESHAP). DESIGN AND METHODS We retrospectively analyzed 163 patients with relapsed or refractory diffuse large B-cell lymphoma who received R-ESHAP as salvage therapy with a curative purpose. Patients were divided into two groups according to whether rituximab had been administered (n=94, "R+" group) or not (n=69, "R-" group) prior to R-ESHAP. RESULTS Response rates were significantly higher in the R- group in the univariate but not in the multivariate analysis. In the analysis restricted to the R+ group, we observed very low complete remission and overall response rates in patients with primary refractory disease (8% and 33%, respectively), as compared to those in patients who were in first partial remission (41% and 86%) or who had relapsed disease (50% and 75%) (p<0.01 in both cases). Overall, 60% and 65% of patients in the R+ and R- groups, respectively, underwent stem-cell transplantation after the salvage therapy. With a median follow-up of 29 months (range, 6-84), patients in the R+ group had significantly worse progression-free survival (17% vs. 57% at 3 years, p<0.0001) and overall survival (38% v 67% at 3 years, p=0.0005) than patients in the R- group. Prior exposure to rituximab was also an independent adverse prognostic factor for both progression-free survival (RR: 2.0; 95% CI: 1.2-3.3, p=0.008) and overall survival (RR: 2.2; 95% CI: 1.3-3.9, p=0.004). CONCLUSIONS R-ESHAP was associated with a high response rate in patients who were not refractory to upfront rituximab-based chemotherapy. However, the survival outcome was poor for patients previously exposed to rituximab, as compared to in those who had not previously been treated with rituximab.

Free flaps in surgical dermatology. Comparison between fasciocutaneous and myocutaneous free flaps in facial reconstructions.

BACKGROUND Dermatologic surgeons routinely harvest pedicled flaps at distance with an axial or random pattern to repair facial defects. These types of skin flaps are time-consuming and have high economic, social and personal costs. These drawbacks could be avoided with the introduction of a single-step transfer of free flaps to the recipient site, with microvascular anastomosis. OBJECTIVE To demonstrate that better results are obtained with myocutaneous or fasciocutaneous free flaps and which one is more suitable in surgical dermatology. MATERIAL AND METHODS We selected two patients of opposite sexes and similar ages who had undergone Mohs surgery to remove recurrent malignant tumors that were located in the upper cheek bordering the zygomatic zone. The woman was treated with a fasciocutaneous radial free flap and the man with a rectus abdominis free flap. RESULTS Both patients had excellent immediate postoperative outcomes. Complications observed in the male patient were related to a previous pulmonary alteration. The fasciocutaneous radial free flap reconstruction was easier to perform than the rectus abdominis free flap; nevertheless, the radial free flap is very thin and, although the palmaris longus tendon is used, it does not yield enough volume, requiring later use of implants. In contrast, the rectus abdominis free flap transfers a wide flap with enough fat tissue to expand in the future. As for the cosmetic results regarding the donor site, the rectus abdominis free flap produces better-looking scars, since secondary defects of the palmar surface cannot be directly closed and usually require grafting - a situation that some patients do not accept. CONCLUSIONS In surgical dermatology, each case, once the tumor has been extirpated, requires its own reconstructive technique. The radial free flap is suitable for thin patients who are willing to cover their arm with a shirt. The rectus abdominis free flap is best suited for obese patients with deep and voluminous defects, although it is necessary to dislocate the navel from its original position.

Identification and in vitro reactivity of celiac immunoactive peptides in an apparent gluten-free beer.

Gluten content from barley, rye, wheat and in certain oat varieties, must be avoid in individuals with celiac disease. In most of the Western countries, the level of gluten content in food to be considered as gluten-free products is below 20 parts per million measured by ELISA based on specific anti-gluten peptide antibody. However, in beverages or food suffering complex hydrolytic processes as beers, the relative proportion of reactive peptides for celiac patients and the analytical techniques may differ, because of the diversity of the resulting peptide populations after fermentations. A beer below 20 parts per million of gluten but yet detectable levels of gluten peptides by anti-gliadin 33-mer antibodies (G12 and A1) was analyzed. We identified and characterized the relevant peptides for either antibody recognition or immunoactivity in celiac patients. The beer was fractionated by HPLC. The relative reactivity of the different HPLC fractions to the G12/A1 antibodies correlated to the reactivity of peripheral blood mononuclear cells isolated from 14 celiac individuals. Peptides from representative fractions classified according to the relative reactivity to G12/A1 antibodies were identified by mass spectrometry. The beer peptides containing sequences with similarity to those of previously described G12 and A1 epitopes were synthesized and confirmed significant reactivity for the antibodies. The most reactive peptides for G12/A1 also confirmed the highest immunogenicity by peripheral blood mononuclear cell activation and interferon γ production from celiac patients. We concluded that preparative HPLC combined with anti-gliadin 33-mer G12/A1 antibodies were very sensitive and specific methods to analyze the relevant immunogenic peptides in hydrolyzed gluten.

HERV-W polymorphism in chromosome X is associated with multiple sclerosis risk and with differential expression of MSRV.

BACKGROUND Multiple Sclerosis (MS) is an autoimmune demyelinating disease that occurs more frequently in women than in men. Multiple Sclerosis Associated Retrovirus (MSRV) is a member of HERV-W, a multicopy human endogenous retroviral family repeatedly implicated in MS pathogenesis. MSRV envelope protein is elevated in the serum of MS patients and induces inflammation and demyelination but, in spite of this pathogenic potential, its exact genomic origin and mechanism of generation are unknown. A possible link between the HERV-W copy on chromosome Xq22.3, that contains an almost complete open reading frame, and the gender differential prevalence in MS has been suggested. RESULTS MSRV transcription levels were higher in MS patients than in controls (U-Mann-Whitney; p = 0.004). Also, they were associated with the clinical forms (Spearman; p = 0.0003) and with the Multiple Sclerosis Severity Score (MSSS) (Spearman; p = 0.016). By mapping a 3 kb region in Xq22.3, including the HERV-W locus, we identified three polymorphisms: rs6622139 (T/C), rs6622140 (G/A) and rs1290413 (G/A). After genotyping 3127 individuals (1669 patients and 1458 controls) from two different Spanish cohorts, we found that in women rs6622139 T/C was associated with MS susceptibility: [χ2; p = 0.004; OR (95% CI) = 0.50 (0.31-0.81)] and severity, since CC women presented lower MSSS scores than CT (U-Mann-Whitney; p = 0.039) or TT patients (U-Mann-Whitney; p = 0.031). Concordantly with the susceptibility conferred in women, rs6622139*T was associated with higher MSRV expression (U-Mann-Whitney; p = 0.003). CONCLUSIONS Our present work supports the hypothesis of a direct involvement of HERV-W/MSRV in MS pathogenesis, identifying a genetic marker on chromosome X that could be one of the causes underlying the gender differences in MS.

Mutation prevalence of cerebral cavernous malformation genes in Spanish patients.

OBJECTIVE To study the molecular genetic and clinical features of cerebral cavernous malformations (CCM) in a cohort of Spanish patients. METHODS We analyzed the CCM1, CCM2, and CCM3 genes by MLPA and direct sequencing of exons and intronic boundaries in 94 familial forms and 41 sporadic cases of CCM patients of Spanish extraction. When available, RNA studies were performed seeking for alternative or cryptic splicing. RESULTS A total of 26 pathogenic mutations, 22 of which predict truncated proteins, were identified in 29 familial forms and in three sporadic cases. The repertoire includes six novel non-sense and frameshift mutations in CCM1 and CCM3. We also found four missense mutations, one of them located at the third NPXY motif of CCM1 and another one that leads to cryptic splicing of CCM1 exon 6. We found four genomic deletions with the loss of the whole CCM2 gene in one patient and a partial loss of CCM1and CCM2 genes in three other patients. Four families had mutations in CCM3. The results include a high frequency of intronic variants, although most of them localize out of consensus splicing sequences. The main symptoms associated to clinical debut consisted of cerebral haemorrhage, migraines and epileptic seizures. The rare co-occurrence of CCM with Noonan and Chiari syndromes and delayed menarche is reported. CONCLUSIONS Analysis of CCM genes by sequencing and MLPA has detected mutations in almost 35% of a Spanish cohort (36% of familial cases and 10% of sporadic patients). The results include 13 new mutations of CCM genes and the main clinical symptoms that deserves consideration in molecular diagnosis and genetic counselling of cerebral cavernous malformations.

Gestão Por Objectivos:Estudo de Caso Realizado nas Escola Segundárias de São Miguel

O presente trabalho aborda a problemática da Gestão da Escola Secundária de Calheta de São Miguel, sobretudo na percepção da gestão feita no respectivo estabelecimento e propor um conjunto de soluções para o melhoramento do seu funcionamento no futuro próximo. O trabalho teve como suporte teórico alguns autores como: Peter drucker, George Odiorne, Paul Mali, Humble, Sebastião Teixeira, Adalberto Chiavenato e outros autores que retratam a problemática da gestão das organizações, focando a relação existente entre gestão das organizações e os resultados.

PROBLEMA DE FREQUÊNCIA DO PRÉ-ESCOLAR NA COMUNIDADE DOS RABELADOS ESTUDO DE CASO: RABELADOS EM ESPINHO BRANCO S. MIGUEL, ILHA DE SANTIAGO:RABELADOS EM ESPINHO BRANCO S. MIGUEL, ILHA DE SANTIAGO

A exploração dos resultados do estudo revela que tanto a informação como o trabalho monográfico que hora se apresenta espelha um estudo elaborado no quadro do curso de Educação de infância da Universidade de Cabo Verde, como uma das exigências para obtenção do grau de Licenciatura, sob a temática “Problema de Frequência do Pré-Escolar na Comunidade dos Rabelados em Espinho Branco S. Miguel – Ilha de Santiago”. Procura-se neste trabalho trazer para debate a problemática de frequência do pré-escolar na Comunidade, bem como o que pensem os pais que não matricularam os seus filhos nesse jardim,ou seja que perspectivas futura para essas crianças e comunidade em geral,visto que há crianças estão dentro do sistema do ensino pré- escolar e que moram em “funcos” tradicionais, assim como outras que se encontram fora do sistema. Convém referir ainda que cinquenta por centro das crianças inscritas no pré- escolar estão registadas e outras não, numa perspectiva de melhoria das prestações auferidas. Centrada em duas abordagens, uma teórica e outra prática, este trabalho projecta de forma resumida alguns pensamentos cujo relevância está na sua estreita ligação com a temática que aqui se desenvolve, sempre em perfeita harmonia com a realidade, objecto do estudo. Conhecer o papel do pré-escolar no desenvolvimento das crianças; conhecer o pensamento dos pais sobre a problemática em estudo. Entre os diversos autores citados, registam-se os trabalhos desenvolvidos por FREDERICO FROEBOL (1783-1852), e Maria Montessori (1870-1952) que proponham peculiares para o desenvolvimento do trabalho pedagógico com crianças; por alguns clássicos psicólogos e sociólogos tais como Piaget (1946-1983), Erickson (1976). Esta rica explanação culmina com uma exploração dos dados resultante de observações, entrevistas cuja pretensão era de auscultar e analisar as opiniões dos diversos intervenientes quanto ao problema de frequência do pré-escolar na comunidade dos rabelados em Espinho Branco S. Miguel – Ilha de Santiago. A frequência do pré-escolar revela como de essencial a contribuir para melhoria de uma comunidade, como tal a dos rabelados em espinho Branco, S. Miguel – Ilha de Santiago.

Capital Social e Políticas Públicas: Análise da implementação do Programa de Luta Contra a Pobreza no Meio Rural nos municípios de São Miguel e Tarrafal

Este trabalho foi desenvolvido no quadro da realização do Mestrado em Ciências Sociais pela Universidade de Cabo Verde. Tem como objectivo analisar as possibilidades e os limites de concretização da estratégia de redução da pobreza adoptada pelo Programa de Luta Contra a Pobreza no Meio Rural que considera o capital social como recurso para o desenvolvimento das comunidades. Em específico, procura analisar como essa estratégia é apropriada e implementada pelas associações comunitárias de desenvolvimento e o impacto das actividades desenvolvidas por essas associações no capital social e na redução da pobreza nos municípios de São Miguel e Tarrafal na ilha de Santiago. A recolha de dados foi feita, basicamente, junto de líderes de associações comunitárias de desenvolvimento e de chefes de agregados familiares, com base num guião de entrevista e num questionário previamente elaborados. Em termos teóricos, a concepção de capital social formulada por Robert Putnam constitui o eixo analítico do presente trabalho. Os dados recolhidos apontam que para além dos inquiridos não assumirem a ideia de comunidade preconizada pelo Programa de Luta Contra a Pobreza no Meio Rural, que as actividades desenvolvidas pelas associações comunitárias de desenvolvimento não contribuíram para um reforço significativo do capital social das comunidades abrangidas pelo estudo, não obstante reconhecerem que essas actividades tiveram um impacto positivo na redução da pobreza.

Insurrection de Naples en 1647. T. 1 / étude historique de Don Angel de Saavedra, duc de Rivas,... ; ouvrage trad. de l'espagnol et précédé d'une introd. par le baron Léon d'Hervey de Saint-Denys

Comprend : Diverses réflexions touchant les révolutions de Naples,...

Insurrection de Naples en 1647. T. 2 / étude historique de Don Angel de Saavedra, duc de Rivas,... ; ouvrage trad. de l'espagnol et précédé d'une introd. par le baron Léon d'Hervey de Saint-Denys

Comprend : Diverses réflexions touchant les révolutions de Naples,...

Estratégias de Superação das Dificuldades da Leitura e da Escrita na II Fase do Ensino Básico em São Miguel: O Caso do Pólo Nº II de Veneza

O presente trabalho que tem como tema “Estratégias de Superação das Dificuldades da Leitura e Escrita na II Fase do Ensino Básico em São Miguel: O Caso do Pólo Nº II de Veneza”, faz uma abordagem geral sobre dificuldades de aprendizagem da leitura e da escrita na 2ª fase do ensino básico com enfoque no Concelho de S. Miguel, particularizando o Pólo Nº II de Veneza, realçando as principais causas dessas dificuldades e por fim propor estratégias de superação das mesmas.