De libris deperditis in S. V. T. codice citatis

Dedicatio: Daniel Ithimaeus [ruots. pr.].

De aspide surda, Psalm. LVIII: v. 5, 6

Invocatio: D.D.

De terminis ventorum, occasione dicti, Joh. III:v.8

Invocatio: D.F.G.

De effato Salvatoris Joh. V: v. 39. Ereunate tas grafas &c

Dedicatio: Carolus Frid. Mennander.

De Aegyptiis & Cuschaeis ad Deum conversis, Psal. LXVIII: v. 32

Invocatio: D.A.G.

Determinação de ácido valpróico em soro por cromatografia líquida de alta eficiência com detector de arranjo de diodos (CLAE-DAD), após derivatização com brometo de fenacila

Valproic acid (VA) is a drug used to control seizures in several epileptic conditions. In VA pharmacotherapy, therapeutic drug monitoring is recommended to obtain adequate seizure control and avoid toxicity. The aim of this study was to validate a method for the determination of valproic acid in serum, employing high performance liquid chromatography with diode array detection (HPLC-DAD), after derivatization with phenacyl bromide. The calibration curve (y=0.0133x-0.0025) presented good linearity with r²=0.9999. Accuracy (101-115%), intra-assay precision (4.53-8.15%) and inter-assay precision (3.15-6.77%) were acceptable. The quantification limit was 2.0 µg/mL. The method presented similar results to enzyme immunoassay.

De petitione Naamanis Syri, II. Reg. cap. V. vers. 18

Invocatio: D.D.

Metathesin ton saleuomenon, Hebr. cap. XII: v. 27

Invocatio: I.N.J.

Kainàn tû Arfaksád [kreikkaa] Luc. 3. v. 36

Invocatio: In nomine Jesu.

De libris deperditis in S. V. T. codice citatis

Arkit: 1 arkintunnukseton lehti, C4 D2.

De Canaane servo servorum, Gen. IX: v. 25

Invocatio: Q.F.F.Q.S.

Determinação de etanol e voláteis relacionados em sangue e fluido oral por microextração em fase sólida em headspace associada à cromatografia gasosa com detector de ionização em chama

The aim of this study was to validate a method for the determination of acethaldehyde, methanol, ethanol, acetone and isopropanol employing solid-phase microextraction associated to gas chromatography with flame ionization detection. The operational conditions of SPME were optimized by response surface analysis. The calibration curves for all compounds were linear with r² > 0.9973. Accuracy (89.1-109.0%), intra-assay precision (1.8-8.5%) and inter-assay precision (2.2-8.2%) were acceptable. The quantification limit was 50 µg/mL. The method was applied to the meaurement of ethanol in blood and oral fluid of a group of volunteers. Oral fluid ethanol concentrations were not directly correlated with blood concentrations.

Daño oxidativo en la microalga Pseudokirchneriella subcapitata expuesta a aguas receptoras de un efluente minero en del Río Blanco (V Región, Chile)

In this investigation antioxidant response and toxicity of metals in receiving water effluent miner in the Blanco river in Pseudokirchneriella subcapitata was assessed. The catalase activity, lipid damage through Tbars, the growth rate of was determined. The result showed an inhibition of the growth rate of P. subcapitata which correlated with increased catalase activity and the lipid liperoxidation. These responses were correlated with the concentrations of copper and iron.

De fundamento telluris; ex occasione Job. XXVI. v. 7

Invocatio: Divina favente gratia.

Determinação de citrato de sildenafila e de tadalafila por cromatografia líquida de ultraeficiência com detecção por arranjo de diodos (CLUE-DAD)

A simple ultra-performance liquid chromatographic method for the simultaneous determination of sildenafil citrate and tadalafil was developed and validated. Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1:10) with methanol. Analysis was performed in an Acquity® UPLC system with Acquity® BEH C18 column (2.1 x 50 mm, with 1.7 μm particles). The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min. The method presented adequate specificity, linearity, precision and accuracy and allowed the determination of the drugs in seized forensic samples.