Shortspine thornyhead (Sebastolobus alascanus) abundance and habitat associations in the Gulf of Alaska

Shortspine thornyhead (Sebastolobus alascanus) abundance was estimated from 107 video transects at 27 stations recorded from a research submersible in 1991 off southeast Alaska at depths ranging from 165 to 355 m. Numbers of invertebrates in seven major taxa were estimated, as was substrate type. Thornyhead abundance ranged from 0 to 7.5/100 m2, with a mean of 1.22/100 m2, and was positively correlated with depth and amount of hard substrate. Invertebrate abundances were not significantly correlated with numbers of thornyheads. Shortspine thornyhead abundance estimates from this study were several times higher than estimates produced by bottom trawl surveys off southeast Alaska in 1990 and 1993, the two years of survey that encompassed the submersible transects; however, the trend of increasing abundance with depth was similar in the trawl surveys and in the submersible transects, suggesting that trawl surveys systematically underestimate abundance of shortspine thornyheads

The protein, peptide and free amino-acid contents of some species of Gracilaria from south-east coast of India

Four species of Gracilaria are investigated for their free amino-acid contents, as well as amino-acid constituents in the proteins and the peptides, using quantitative paper chromatographic technique. Amino-acid constituents of different species of Gracilaria differ only in amount, while free amino-acids and the amino-acids in the peptides vary both in quality and quantity. A number of amino-acids recorded as protein constituents have even escaped detection in the peptides, while in the free state they occur either in all the species or in some only except homocystine. Moreover, some amino-acids occur exclusively in the free state.

The free and combined amino acid contents in species of Caulerpa from southeast coast of India

Caulerpa racemosa var. macrophysa, C. racemosa var cornyphora and C. scalpelliformis are analyzed by quantitative paper chromatographic technique for their amino acid contents in proteins, peptides and free state. It is found that no appreciable variation occurs in the quality of amino acid make up in these algae; but quantitative differences are apparent in them. Moreover, both qualitative and quantitative variations occur in the amino acid make up of the peptides, and in the free state. The results are compared with those of the other investigations.

Los elefantes marinos de península Valdés

Durante la primavera austral, la Península Valdés (Chubut, Argentina) es el escenario de la masiva visita del elefante marino sudamericano (Otaria flavescens). Se trata de la temporada reproductiva. El recuento año tras año de este conjunto migrante es un trabajo indispensable para conocer la dinámica de la población. Los censos poblacionales terrestres ofrecen un invalorable conocimiento de sus fluctuaciones y migraciones. Se brindan particularidades de la elefantería de Valdés y sus poblaciones, aspectos biológicos relevantes y otras lecturas sugeridas.

Investigation of extinction in unsteady flames in turbulent combustion by 2D-LIF of OH radials and flamelet analysis

An experimental and theoretical investigation of premixed turbulent combustion in an engine simulator is presented. The distribution of hydroxyl radicals formed in the combustion of propane/air mixtures was visualized by 2D-LIF and used to monitor the progress of the combustion process. For stoichiometric mixtures, images showed a continuous wrinkled flame front, while in lean (λ=1.5) mixtures, local flame extinction was observed as discontinuities in the reaction zone. A bright active reaction zone was still observed in flame inlets and closed concave structures. The effects of self-absorption and of collisional quenching on the fluorescence signal are considered and appear to have only a minor net influence on the shape and width of the flame front. The images are evaluated and interpreted in terms of the Lewis number effect and the laminar flamelet model. Analysis was performed by determining the contour lines of the images (specifically, the ratios of average maximum to equilibrium OH concentration) and comparing with corresponding ratios from unstrained flame simulations. The results show that although the degree of turbulence is not high enough for straining effects to be important, flamelet curvature does play a significant role in the combustion of lean mixtures; this is manifested by a mean effective flame velocity that is less than the laminar burning velocity. © 1991 Combustion Institute.

从电鳐电器官中提取N2-AchR纯品建立EAMG模型

下载PDF阅读器目的 从厦门海域单鳍电鳐的电器官中提取烟碱型乙酰胆碱受体(N2-AchR)纯品,用纯化的N2-AchR 免疫Lewis大鼠,建立实验性自身免疫性重症肌无力(EAMG)动物模型.方法 参照文献报道[1-2]从电鳐电器官提取N2-AchR纯品,并采用SDS凝胶电泳蛋白定性鉴定及考马斯亮兰定蛋白含量;以纯提的蛋白主动免疫Lewis大鼠,共免疫3次,于末次免疫后第3日进行EAMG大鼠临床评分及攀网时间、肌电图、血清Nz-AchRab含量、突触后膜上N2-AehR数目的 检测.结果 纯化的蛋白含量为1.097m咖l(标准品为1mg,ml);EAMG模型组与佐剂组比较,攀网时间明显降低(p<0.01);临床评分及肌电图第五个反应幅度衰减百分率显著升高(P<0.01);血清N2-AchRab含量明显增加(P<0.01);突触后膜上Nr-AchR数目显著减少(P<0.01).结论 从单鳍电鳐电器官纯提的Nz-AchR蛋白成功诱导EAMG模型,为进一步研究重症肌无力创造了良好条件.

眼镜蛇毒因子消耗补体对大鼠移植心急性排斥反应的抑制作用

目的 研究中华眼镜蛇毒因子(CVF)消耗补体对大鼠心脏移植急性排斥反应的影响.方法 以近交系BN大鼠为供者,Lewis大鼠为受者,建立腹腔异位心脏移植模型.实验分为2组,每组8只.CVF组:心脏移植术前3 d、2 d时,经受者尾静脉注射CVF 50 μg/kg;术前12 h至移植心停跳时,经尾静脉注射CVF 20 μg/kg,每2 d注射1次.对照组:术前、术后不给予受者任何特殊处理.术后观察移植心的存活时间,测定术后第1、3、5和6d及移植心停跳时的血清总补体活性(CH50法)、移植心C3沉积及CD3+T细胞浸润情况,并观察移植心的病理变化.结果 CVF组和对照组的移植心存活时间分别为(11.69±0.72)d和(6.65±0.35)d,两组比较,差异有统计学意义(P＜0.01).CVF组移植心组织内C3沉积和CD3+T细胞浸润程度均较对照组同期明显减轻,病理损害程度也较对照组同期明显减轻.结论 CVF消耗补体对大鼠心脏移植急性排斥反应起到了明显的抑制作用,从而延长移植心存活时间.

云南眼镜蛇毒因子用于预致敏大鼠同种心脏移植的实验研究

目的观察纯化的云南眼镜蛇毒因子(Y-CVF)对预致敏大鼠同种心脏移植急性体液排斥反应的作用。方法BN大鼠到Lewis大鼠连续3次皮肤移植预致敏后行颈部异位心脏移植。将15对大鼠用随机数字法分成2组,实验组(n=8)于心脏移植前24h静脉给予Y-CVF80μg/kg;对照组(n=7)不用Y-CVF。观察移植心的生存时间,移植心停跳后病理学检查排斥类型,免疫组织化学染色观察移植心IgG和补体C3的沉积。结果Lewis大鼠预致敏后抗BN大鼠抗体滴度由0升高至1∶1028~1∶2056。对照组移植心存活时间为12·71h±13·94h,实验组移植心存活时间为99·50h±38·72h,与对照组比较差异有统计学意义(t=5·599,P<0·01)。病理检查结果证实,实验组均未发生急性体液排斥,仅见以大量单核淋巴细胞浸润为特征的急性细胞排斥反应。对照组则见以小血管内血栓形成为特征的急性体液排斥反应。免疫组织化学IgG染色实验组和对照组均为阳性,C3染色对照组为阳性,而实验组为阴性。结论使用Y-CVF可克服预致敏大鼠同种心脏移植急性体液排斥反应的发生。

User responses to the learning demands of consumer products

Designers are typically male, under 35 years old and unimpaired. Users can be of any age and currently over 15% will have some form of impairment. As a result a vast array of consumer products suit youthful males and in many cases exclude other demographics (e.g. Keates and Clarkson, 2004). In studying the way a range of users learn how to use new products, key cognitive difficulties are revealed and linked back to the areas of the product causing the problems. The trials were structured so each user had to complete a specific set of tasks and were consistent across the user spectrum. The tasks set aimed to represent both everyday usage and less familiar functions. Whilst the knowledge gained could provide designers with valuable guidelines for the specific products examined, a more general abstraction provides knowledge of the pitfalls to avoid in the design of other product families.