Mitochondrial cytochrome b gene sequences of Old World monkeys: With special reference on evolution of Asian colobines

The classification and phylogenetic relationships of the Old World monkeys are still controversial. For Asian colobines, from three to nine genera were recognized by different primatologists. In the present study, we have sequenced a 424 bp mitochondrial tRNA(Thr) gene and cytochrome b gene fragment from Macaca mulatta, Mandrillus sphinx, Mandrillus leucophaeus, Semnopithecus entellus, Trachypithecus vetulus, T. johnii, T. phayrei, T. francoisi, Pygathrix nemaeus, Rhinopithecus roxellanae, R. bieti, R. avunculus, Nasalis larvatus, and Colobus polykomos in order to gain independent information on the classification and phylogenetic relationships of those species. Phylogenetic trees were constructed with parsimony analysis by weighting transversions 5 or 10 fold greater than transitions. Our results support the following conclusions: (1) the Old World monkeys are divided into two subfamilies; (2) that among the colobines, Colobus, the African group, diverged first, and Nasalis and Rhinopithecus form a sister clade to Pygathrix; (3) that there are two clades within leaf monkeys, i.e. 1) S. entellus, T. johnii, and T. vetulus, and 2) T, phayrei and T. francoisi; (4) that Rhinopithecus avunculus, R. roxellanae, and R. bieti are closely related to each other, and they should be placed into the same subgenus; (5) that Rhinopithecus is a distinct genus; and (6) that the ancestors of Asian colobines migrated from Africa to Asia during the late Pliocene or early Pleistocene.

An analytical study of the sea water slime with reference to bacterial flora

A detailed study of the primary film of slime with reference to the bacterial flora, its quality and quantity, as observed on different material surfaces exposed to sea water is carried out. A correlation has been established between bacteria and slime deposited on different surfaces.

Ice storage characteristics of perch with special reference to its suitability for canning

Perch (Pagrus spinifer), one of the most abundantly available fishes of Gujarat coast, was subjected to a detailed study for assessing its storage life in ice and amenability of the iced fish for canning. Changes in the salt soluble nitrogenous material and myosin content of the iced fish showed good correlation with the changes in the organoleptic and physical qualities. The fish was found to have a storage life of 9 days in ice and samples stored up to 7 days were suitable for canning.

Taxonomic problems in the Drosophila melanica species group (Diptera : Drosophilidae) from southern China, with special reference to karyotypes and reproductive isolation

Karyotypes and reproductive isolation were studied in two allopatric populations of Drosophila tsigana, one from Guizhou Province in southern China and the other from Hokkaido in northern Japan, and in one population of a closely related species, D. longi

Height-depth (breadth) relationship of shells in the backwater oyster Crassostrea madrasensin (Preston) of the Cochin harbour

A study of the height-depth relation in the Indian backwater oyster Crassostrea madrasensis (Preston) was carried out. The plot of height against depth showed an exponential trend and a relationship of the form H = ADB. Plot of height against depth also showed larger deviations in height for oysters with greater depth. Analysis showed that variations in height do not result in corresponding variations in depth, particularly in oysters with increased height.

Preservation of Sardinella longiceps in iced and chilled seawater. Part 1 - Changes during storage with particular reference to bacterial load and nitrogenous compounds

Oil sardines in prime condition were subjected to onboard chilling. Two lots were chilled in CSW (samples C and CI), a third lot was chilled in crushed ice (sample I) and a fourth lot left not iced on deck (Sample AI). Upon landing sample AI was iced and sample CI was removed from the CSW and iced. All the four samples were kept in a chilled room for storage studies. The fish chilled and stored in CSW recorded the least, and the fish subjected to delayed icing, the highest values for all the indices of spoilage namely, free amino nitrogen, trimethylamine (TMA) and total volatile base nitrogen (TVBN). The total psychrophilic bacterial number also showed a similar trend. The organoleptic assessment of the cooked samples revealed C I, CI, AI to be the order of preference throughout the storage. This assessment was found to hold good for the rest of the parameters as well. The CSW held fishes were found to be distinctly superior to the iced ones for the first five days of storage. Such a marked prevalence in quality for five days would suffice for the fish to fetch a premium in the market over other landings of the same fish whether chilled or not chilled. Chilling on board in CSW and icing the same after landings, did not show encouraging results.

Preservation of Indian oil sardine (Sardinella longiceps) in iced and chilled seawater. Part 2 - Changes during storage with particular reference to salt penetration and lipid deterioration during CSW holding

Oil sardines in prime condition were chilled on board. Two lots were chilled in CSW (samples C & CI), one lot ice (sample I) and a fourth lot was left un-iced on deck (sample AI). Sample AI was iced after landing and sample CI was taken out of the chilled seawater and. iced. All the four samples were kept in a chilled room for storage studies. Sample C, chilled and stored in CSW, recorded a gradual gain in weight and an increase in salt content of the muscle. Presence of salt did not seem to cause any excessive protein denaturation. Salt extractability decreased at a gradual rate in all cases. Presence of salt seemed to wield no noticeable influence on lipid hydrolysis and subsequent peroxidation. Results of chemical and sensory evaluations highlight this. Holding sardines in CSW gave a product of excellent quality for the first four to five days of storage. Beyond the fifth day of storage quality deteriorated rapidly and there was no noticeable superiority for this sample (sample C) over the on board iced fish. This was evident in the sensory evaluation as well. However, a storage life of five days in a readily acceptable state is sufficient for the fish to be disposed in the market at a premium sale price over other landings of the same species.

Hydrobiological studies of an irrigation tank with reference to the culture of large murrel, Channa marulius (Hamilton) and striped murrel Channa striatus (Bloch)

The importance of selection of species for culture according to the ecological niches and fish food organisms is highlighted with respect to the Fox Sagar, an irrigation take. The tank was infested with submerged vegetation as well as minnows and weed fishes, which rendered the tank unsuitable for the culture of Indian major carps. The tank was stocked with 8000 fingerlings of Channa marulius and C. striatus during 1981 by the local fisherman co-operative society. Only partial harvest was possible during 1982 because of high water level. The final harvest was in April-May, 1983. The yield obtained was 3640 kg during the culture period of about 20 months.

Diversity, abundance distribution and secondary production of intertidal and subtidal benthic macrofauna of Bushehr Province with emphasis on annelida groups

The present investigation was undertaken to establish a reference situation for future use, to identify temporal and spatial composition of macrofauna and estimate some ecological indices in the sub tidal waters along the Bushehr coastal waters in Persian Gulf. Six transects were selected including Genaveh, Farakeh, Shif, Bandargah, Rostami and Asalouyeh, at each transect 3 station were sampled in depths of zero, 5 and 10 metres. Sampling was seasonally carried out by a VAN VEEN grab 0.0225 m2, during summer 2008 until spring 2009. Samples were wet sieved immediately using 0.5 mm mesh size sieves and sediment retained in the sieve was preserved in 4% buffered formalin solution. Macrofauna specimen were separated from the sediments using decantation and elutriation methods, enumerated and identified up to the Genus level. Environmental factors such as temperature. pH, and salinity were recorded in field using sensitive probs and refractometer (for salinity) and also sediment samples were taken for TOM and grain size analysis in all the stations. 5611 specimens belonging to 66 genera were collected during the present study. Polychaetes were dominant both in terms of genus number (31) and relative abundance (74 % of total macrofaunal abundance). The other dominant groups were Artheropoda, (16.1%), Molusca (2.8%), Echinodermata (1.29%) and others including Nematoda, Nemertina, Echiura and Turbellaria (5.8%). Thirty one Genera belong of 27 families of polychaeta, one genus and family of Subphylum Chlicerata,19 genera belong to 14 families of Crustacea, 8 genera belong to 6 families of Molusca, were indentified in the studied region. 1 family (Polygordidae) and 3 genera (Flabeligera, Pilargis and Polygordius) of Polychaeta, 1 family (Nymphonidae) and genus (Nymphon) of Chelicerata, 1 Family (Nematoplanidae) and genus (Nematoplana) of Turbellaria, were identified for the first time in Persian Gulf area. The result indicated that macrofauna organism have strong relationship with the grain size characteristics of the sediments they inhabit. The most surface deposit feeder specimens such as Prionospio and Cossura were found in zero meters depth of Genaveh, Farakeh, Bandargah, Rostami and Asalouyeh stations with sandy substratum, however the most burrowing deposit feeder and scavenger specimens such as Capitella and Petaloproctus were collected in 5 and 10 meter depths of stations with silty–clay substratum. The annual mean abundance, Shanon- weiner diversity and evenness of macrofauna were estimated1152.73 N/ m² , 2.72 and 0.792 respectively .The annual average biomass and secondary production were computed 1.797 gDW m² and 3.594 gDW m² y-1 .The average of water temperature, salinity, pH and oxygen concentration were recorded between 16.37-36.05 °C, 38-42 g/l, 7.89-8.76 and 4.23-8.23 mg/l, respectively during this study in 6 studied region. Among of investigated stations Asalouyeh adjacent of effluent canal of Gas and petrochemical industry sewage and Farakeh regions adjacent the Helleh estuary had the lowets and the highest community indices. The average of diversity and density in 5 meters depth stations with moderate of sand, silt and clay were slightly more than 2 other depths stations, it seems that 5 meters stations are made a transition habitats between 2 sandy and clay habitats, that can be used by 2 groups of surface and borrowing deposit feeders. Based on the data provided in this survey, the temperature variation, sediment texture, TOM, type habitat and manmade factors of Gas and petrochemical industries have had the most effect on the macrofauna community structure in the studied region during sampling periods.

A review of spider majid crabs of the northern Arabian Sea, with particular reference to Pakistani species, and with description of an undescribed Pleistacantha

The present report deals with the history of work done on northern Arabian Sea majids, their zoogeographical consideration and recognition of present status of certain genera which are established by recent workers. I have nothing of substance to add to previous treatment by Griffin & Tranter (1986). Emphasis is given to species inhabiting coastal waters of Pakistan. The occurrence of the genus Pleistacantha in the Arabian Sea and its adjacent gulfs is discussed in detail due to presence of a unique female which was taken by Fridtj of Nansen Cruise in 1977. This seems to be an undescribed species. At the moment the female is just given as Pleistacantha sp1 till more specimens obtained determine its position. Some interesting features of Doclea muricata are also considered.

Technical report on the environmental monitoring of the cage area at the Source of the Nile (SON) Fish Farm for quarter 4: October-December 2011

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested for technical assistance of NaFIRRI to undertake regular environment monitoring of the cage site as is mandatory under the NEMA conditions. As the SON is a key collaborator/client of the institute, NAFIRRI agreed to undertake the assignment subject to facilitation by the client. The institute agreed to conduct quarterly surveys of key environmental parameters at the site including selected physical-chemical and biological factors, nutrient status, column depth, water transparency and sedimentation. Samples and field measurements were to be taken at 3 sites: within and/or close to the fish cages (WIC), upstream (USC) and downstream (DSC) of the cages. The first environmental monitoring survey was undertaken in February 2011; the second in May 2011 and the third in September 2011. The surveys cover physical-chemical parameters, nutrient status, invertebrate and fish communities. The present report presents field observations made for the fourth quarter survey undertaken in November 2011 and provides a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and the different aquatic biota at and around the cage site including natural fish communities.