A simplified procedure for determination of clofentezine residues in fruits by liquid chromatography with UV detection

A rapid and sensitive method is described for the determination of clofentezine residues in apple, papaya, mango and orange. The procedure is based on the extraction of the sample with a hexane:ethyl acetate mixture (1:1, v/v) and liquid chromatographic analysis using UV detection. Mean recoveries from 4 replicates of fortified fruit samples ranged from 81% to 96%, with coefficients of variation from 8.9% to 12.5%. The detection and quantification limits of the method were of 0.05 and 0.1 mg kg-1, respectively.

The influence of fruit morphology and habitat structure on ant-seed interactions: A study with artificial fruits

Artificial fruits designed to simulate lipid-rich non-myrecochorous diaspores were used to test for the effect of fruit morphology and habitat structure on ant-seed interactions in an Atlantic Forest site in SE Brazil. The outcome of the interaction (i.e., if the fruit was removed, cleaned by ants on the spot or had no interaction with ants) and the time of ant response were the investigated variables. Models simulating drupes and arilate diaspores were used to test for morphological effects and four habitat attributes (litter depth, number of logs, number of trees, and percentage of bromeliad coverage on the forest floor), likely to be correlated with the ant diversity and abundance in the study site, were measured to test for the effect of habitat structure. The proportion of fruits removed or cleaned did not differ between the two morphological models. Sites in which fruits were cleaned had more trees than those in which no interaction occurred. This may be a result of the foraging behavior of arboreal ants that frequently descend to the forest floor to exploit fleshy diaspores. Sites in which model removal occurred had lower litter depth than both those in which models were cleaned and those in which no interaction occurred. A negative correlation was observed between litter depth and ant response time. Accumulation of leaf litter at a given point may have constrained the movements of large ants in general, and ponerine ants (that are important seed removers) in particular. We conclude that that local pattern in litter depth and tree density influence the frequency and outcome of interactions between ants and non-myrmecochorous, fleshy diaspores.

Autocorrelação espacial em população natural de Terminalia argentea Mart et Succ. no cerrado de Selvíria, MS

The objective of this study was determine the spatial distribution of genotypes of Terminalia argentea Mart et Suce. (Capitão-do-campo) in a natural population, aiming to outline strategy to genetic conservation in situ and ex situ. The population (Terminalia argentea) is located in an area of cerrado on the Teaching and Research Farm of FEIS / UNESP. It was sampled seeds in 30 trees to determine the biochemistry and technological traits. The trees were also located per GPS apparatus, with objective of obtaining geographic coordinate and to analysis the genotype spatial structure from I Moran Index. The analysis of spatial autocorrelations, from I Moran index, indicated the tendency of a larger structure among trees near spatially. In another hand, trees distant spatially showed smaller similarity. The spatial structure was more visible in a ray of 353m.

An alternative LC-UV procedure for the determination of prochloraz residues in fruits

An alternative method using liquid chromatography with UV detection for the determination of prochloraz as 2,4,6-trichlorophenol in mango, papaya and orange is described. Ethyl acetate, acetone and dichloromethane were tested for extraction of prochloraz from the fruits. After extraction the residue of prochloraz was derivatized with pyridine hydrochloride. The analysis was carried out using liquid chromatography with UV detection and gas chromatography with electron-capture detection. Average recoveries of prochloraz from spiked fruits (0.1 and 0.2 mg kg-1) ranged from 80% to 94% with relative standard deviations between 5.6% and 12.6% (n=8). Detection and quantification limits were 0.05 and 0.1 mg kg-1, respectively. The LC-UV method was applied to mango and papaya samples submitted to dip treatment with a prochloraz formulation under laboratory conditions. In addition, fruit samples obtained from local markets were analysed. ©2005 Sociedade Brasileira de Química.

Plantas do cerrado brasileiro com atividade contra Mycobacterium fortuitum

Mycobacterium fortuitum is a rapidly-growing species of bacteria, ubiquitous in the environment and related to important human mycobacterioses. It has been isolated from blood, abscesses, the endocardium and surgical and traumatic wounds. This mycobacterium is hard to treat, being recognized in the literature as resistant even to the drugs used in the treatment of tuberculosis. The objective of this study was to screen extracts prepared from plants of the Brazilian cerrado (extended savanna-like belt) with known activity against M. fortuitum, employing the Microplate Alamar Blue Assay (MABA) as the analytical method. Out of 26 extracts tested against M. fortuitum, the nonpolar extract of Quassia amara (in methylene dichloride) gave the best result (MIC 62.5μg/ mL), followed by the nonpolar extracts of Syngonanthus macrolepsis, Davilla elliptica and Turnera ulmifolia, with equal MICs of 125μg/ml. The polar extracts (in ethanol and methanol) obtained from the same plants were considered inactive, since the MIC values determined were above 500μg/mL and not significantly different from those of extracts from other plants, without known activity.

Minimally processed guava Fruits (Psidium guaiava L.)

'Paluma' guavas, after internal quality evaluation using magnetic resonance tomography, were used to produce fresh-cut product. Fruits were peeled or not, cut in halves and seed removed, and they were packaged in polystyrene trays covered with PVC film or in a PET container with a lid. These packages were stored for 12 days at 5°C, 10°C and ambient temperature (22.6°C). Tomography evaluation verified that impacts produced internal bruising with loss of cellular integrity and liquefication of the placenta tissues. Compression was more evident on the pericarp and cutting promoted superficial deformation. Storage temperature affected the weight loss, with fruit packaged in the polystyrene tray having a greater weight loss. The peeling did not influence weight loss. Product stored at 5°C and 10°C for 8 days had low microbial growth (<103 UFC.g-1) and no coliforms. Rapid spoilage and a short shelf life (3-4 days) occurred when the product was stored at ambient temperature. Peeling reduced ascorbic acid concentration and total soluble solids. Use of calcium to protect fresh-cut products was not efficient. Calcium absorption capacity of 'Pedro Sato' guava was tested using 45Ca. Fruits treated with 2% CaCl2, with or without the radioisotope, were divided in four layers (epicarp, mesocarp, endocarp and seed) and analyzed for the total and 45Ca calcium. It was observed that the applied calcium remained in superficial layers of45fruits, which was confirmed by autoradiography. Internal layers did not contain 45Ca, indicating that calcium was not distributed into different parts of the fruit.

Fenologia e artrópodes de Copaifera langsdorffii Desf. no cerrado

There was evaluated the phenology and arthropods as well as the effect of weather on copaiba (Copaifera langsdorffii Desf.) (Leguminoseae). The emission of new leaves and inflorescences as well as the opening of them occurred in the rainy period. There were observed the higher diversity and abundance of insects, principally galling insects.

Physical and chemical characterization of dovyalis fruits

The objective of this work was to establish physical and chemical characterizations of dovyalis hybrid fruits (Dovyalis abyssinica and D. hebecarpa). Samples of 25 fruits were characterized by evaluation of length and width, weight, percentage and number of seeds per fruit, peel percentage, pulp percentage, total soluble solids (TSS), titratable acidity (TA), vitamin C and TSS/TA ratio. Dovyalis fruit has good physical quality for market with an average of 75% pulp. Vitamin C content averaged 120.3 mg/100 g of fresh fruit, characterizing dovyalis as a good source of vitamin C. © 2005 by The Haworth Press, Inc. All rights reserved.

Storage in cerrado soil and germination of Psychotria vellosiana (Rubiaceae) seeds

The regeneration of plant communities from seed depends, to a large extent, on the capacity of the seed remaining viable in the soil. The viability and germination of artificially buried Psychotria vellosiana seeds in cerrado soil were studied, with the purpose of discovering some physio-ecological aspects of dispersed seeds and evaluating their potential to constitute a soil seed bank. Seed samples were placed in nylon envelopes and buried in the soil of a Cerrado reserve at two different depths and sites. Buried seeds were retrieved periodically and tested for germination along with dry-stored seeds. In general, there was a reduction in seed germination with storage time, both in soil and dry stored conditions, and in some assays exhumed seeds germinated faster than dry stored ones. In general the soil storage favoured seed viability of ungerminated seeds as compared to dry stored ones, with the seeds remaining partially viable after 10 months of storage. The lack of germination of viable seeds suggests that seeds showed true dormancy and/or required an extended time to germinate. It was observed that some seeds had germinated while buried and such in situ germination tended to increase with rainfall. The water availability in the soil might be a limiting factor for successful germination of P. vellosiana in the field, and the seeds may constitute a persistent soil seed bank in the cerrado as dispersed seeds remain viable in the soil until the following period of seed dispersal.

Dinâmica da regeneração do subsolo de áreas degradadas dentro do bioma Cerrado

The objective of this research was to evaluate the physical and chemical conditions of revegetated subsoil in degraded areas in a Cerrado biome and to verify which plants promote better conditions for soil recovery. The research was conducted in the remaining area of the hydroelectrical plant site at Ilha Solteira (SP). The experimental design was a completely randomized with five treatments and three replications. The treatments consisted of: natural regeneration area, brachiaria area, Pinus sp. area, exposed soil area and Cerrado grassland (used as control). The following soil characteristics were appraised: porosity; density, stability of aggregates, infiltration rate, temperature, exchangeable cations, organic matter, pH and potential acidity. The results show that Pinus is not a good species to recover the chemical attributes of the subsoil. The revegetated areas need to be improved in their physical attributes to allow a better development of the vegetation. Brachiaria and the natural regeneration were the most promising treatments, presenting results similar to natural Cerrado.

Carotenoid composition of Brazilian fruits and vegetables

Brazil has a wide diversity of food sources of carotenoids. The updated Brazilian database consists of more than 270 items of fruits, vegetables and their prepared and processed products. The database demonstrates variations due to variety, maturity, production technique, climate and processing. Many of these foods are not found in the US and European databases. Good to rich sources (>20 μg/g) of β-carotene are: acerola, bocaiúva, mango 'Extreme' and tucumã. Sources of both α-carotene and β-carotene are buriti, carrot, Cucurbita moschata 'Menina Brasileira', 'Baianinha' and 'Goianinha', and red palm oil. Commercially produced and uncultivated or semi-cultivated leafy vegetables, C. maxima 'Jerimum Caboclo' and the hybrid Tetsukabuto, cooked broccoli are sources of lutein and β-carotene. The edible Tropaeolum majus flower is especially rich in lutein. Although many fruits have β-cryptoxanthin as principal carotenoid (e.g. caja, nectarine, peach, orange-fleshed papaya, tree tomato), the levels are below 20 μg/g. Good to rich sources of lycopene are guava and guava products, papaya, pitanga and pitanga juice, tomato and tomato products, and watermelon. Sources of zeaxanthin are rare; although the principal carotenoid of piqui, the amount is low, lower than that found in buriti.

Flebotomíneos (Diptera, Psychodidae) de área de cerrado no Município de Corumbataí, Centro-leste do Estado de São Paulo, Brasil.

Distribution and ecology knowledge of sandfly species is essential for epidemiology vigilance and risk determination for transmission of leishmaniasis. Sandfly trapping was carried out in a cerrado strictu sensu vegetation fragment in a rural area of Corumbataí Municipality, São Paulo State, Brazil, during July to November 2004. Two CDC light traps were used from 18h to 8h, once a month, resulting in 112 total hours of capture. During this period, 60 sandfly specimens of ten different species were sampled. The most abundant species and the one found in all captures was Pintomyia monticola totalizing 15 (25.0%) specimens, the second more abundant one was P. pessoai with 14 (23.3%) and the third one was Brumptomyia guimaraesi with 12 (20.0%) individuals collected. Other captured species were Psathyromyia aragaoi, B. avellari, B. brumpti, B. cunhai, P. bianchigalatiae, Evandromyia termitophila and Nyssomyia whitmani which corresponded to 19 (31.2%) specimens captured. Pintomyia pessoai and N. whitmani presence indicates transmission risk of American Cutaneous Leishmaniasis in the area.

Mutagenic effect of native species of the Brazilian Cerrado with anti-ulcerogenic activity

Neea theifera Oerted (Nyctaginaceae), Guapira noxia Linn. (Nyctaginaceae) and Hancornia speciosa Gomes (Apocynaceae) are plant species found in Brazilian Cerrado used popularly for the treatment of gastric ulcers. Here they are assessed for mutagenic activity by analysis of the reverse mutations induced in Salmonella typhimurium strains TA100, TA98, TA102 and TA97a, by extracts of the plants, with and without metabolic activation. Methanol and chloroform extracts of N. theifera and G. noxia and methanolic and aqueous extracts of H. speciosa were tested at five different concentrations. It was found that only the methanolic extract of H. speciosa exhibited a positive mutagenic effect, on strains TA98 and TA100 in the absence of metabolic activation. The phytochemical analysis of the species suggested that condensed tannins are the main compounds responsible for the observed effect.

Propriedades químicas de um latossolo vermelho de cerrado cultivado com algodão sob diferentes preparos e adubações

The high rate of mineralization of organic matter on savannah soils, which is reached fi ve times faster than in temperate regions, leads us to the challenge of electing the best system of management that maintains and/or increase it in soil, guaranteeing its quality and sustainability. In this sense the present research aimed to study the effects of green, organic and mineral manure on the chemical properties on the chemical properties of an Oxisol, on Savannah area, cultivated with cotton (Gossypium hirsutum) under conventional tillage and no-tillage in the pasture fi eld (Brachiaria decumbens) for 20 years. The experimental design was a randomized block design with split plots. The main plots consisted of two treatments: conventional tillage and no-tillage, and the subplots of six treatments: control (no fertilizer), mineral fertilizer recommended for the crop, according to the soil chemical analysis, organic fertilizer (cattle manure - 20 t ha-1), organic fertilizer (cattle manure - 20 t ha-1) + 1/2mineral fertilization recommended according to the analysis of soil, green manure-1 (Crotalaria juncea) and green manure-2 (Pennisetum americanum). There were studied the following soil chemical properties: P, OM, pH, K, Ca, Mg, Al, Al + H, S, exchange capacity cations and base saturation. The soil samples for the analysis were performed on layers of 0,00-0,05 m, 0,05-0,10 and 0,10-0,20 m. Then it came the following conclusions: the fertilization interfere in soil chemical properties and the preparation did not interfere, the cattle manure and its association with the mineral fertilizer caused increasing level elements in the soil, Crotalaria juncea and Pennisetum americanum did not infl uence on soil chemical properties.