Produção de enzimas ligninolíticas, glioxal oxidase e proteases por fungos marinhos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção e aplicação de ramnolipídios produzidos por Pseudomonas aeruginosa LBI 2A1

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise comparativa entre a produção de biogás em aterro controlado e variações no potencial elétrico natural por meio de perfilagem geofísica

This work aims to analyze data from a Natural Electric Potential landfill type ditch located in Cordeirópolis (SP). The procedure measures initially involved the assembly and installation of an apparatus cables, sensors and drain geophysical measurements and flow rate of biogas in a ditch later filled by solid waste. Biogas is a product of degradation of organic matter in waste by the action of microorganisms in an anaerobic environment. Its features high methane content in the gas potentially usable as fuel in energy generation systems or steam. The study area is characterized by clayey soil composition change from the diabase sill correlated to the event Serra Geral Formation siltstones overlapping Tatuí with groundwater level site around 50m. The cables were installed vertically in parallel with drain gas manifold, which allowed the collection of data by the technique geophysical logging every 15 days over a period of eight months. For data analysis we used four parameters in the study, the closing time of the ditch, natural electric potential, the flow velocity of biogas and rain. With the tabulated data plots were constructed for each collection day showing values in the range of the cable is in contact with waste (200 cm - 400 cm) and range of the cable in contact with the soil (600 cm - 800 cm). Subsequently graphs were generated with the period of eight months to analyze the data tabulated. A statistical correlation data, which show the influence of rainfall on the production of biogas. The results were satisfactory and demonstrated the feasibility of the research method in studies for the feasibility analysis for the capture of biogas energy

Avaliação da toxicidade da vinhaça utilizando Oreochromis niloticus (Perciformes: Cichlidae) como organismo teste

Contamination of rivers and lakes, waste disposed by infiltration into the soil as fertilizer, for example, has been highlighted around the world, since such compounds can contain toxic elements, causing changes in aquatic and/or terrestrial ecosystem. Due to the possible presence of these elements in these wastes, it is possible assess damage to organisms exposed through breaks in genetic material and mutations. In this sense, this work aimed at exploring the cytotoxic, mutagenic and genotoxic vinasse pontential, residue used as fertilizer, from the processing of sugar cane into alcohol, using fish (Oreochromis niloticus - Cichlidae) as a test organism. The individuals were exposed to different dilutions of vinasse in the water and later carried out analysis of their erythrocytes using the micronucleus test and nuclear abnormalities associated and the comet assay. In the first bioassay, tilapia exhibited 5% and 10% vinasse concentrations died after 48h. The number of micronucleated erythrocytes was statistically significant for the 1% vinasse concentration and also for nuclear abnormalities such as broken-egg and nuclear bud, when compared with negative and positive controls; were also significant lobed type erythrocytes, when compared to the negative control. The test results of the comet assay were not significant for 1% vinasse when compared to the negative control, due to the high rate of comets with damage control, which is unusual. The same was observed for changes as notched and blebbed type. In the second chemical analyzes conducted, the detection of heavy metals in the vinasse sample, charged only the presence of copper and chromium, the latter with a concentration 89 times higher than in the first test. Differentially the first bioassay, fish exposed to dilution of 5% did not die... (Complete abstract click electronic access below)

Análise comparativa entre sistemas de resfriamento de injeção de plástico

This study aims to prove the economic feasibility of the installation of mechanical compression chillers on plastic injection molding machines in order to reduce the production cycle time of toothbrush cables in a specific case study. This evidence was confirmed by the comparative analysis of the system replaced and the new system installed. The old system had only one closed loop cooling tower which pumped chilled water to the injection molds, and the new system has the same tower sending cold water to the condensers of individual chillers installed on each injection machine. We conducted an analysis of energy efficiency in each system, showing that in terms of thermal efficiency virtually nothing has changed, but in terms of electricity demand the new system consumes 60.3 kW more. We conducted an analysis of machine productivity for both systems, showing a much higher productivity of the new system due to reduced cycle times caused by the presence of chillers and their greater cooling capacities. Equipped with data such as electricity rates, increases in operating costs and initial investments, the increase in consumption and demand of electricity plus the cycle time reduction were also calculated over so the simple payback 1 year and 2 months was reached

Influência de bioestimulante no desenvolvimento de plantas de pepino japonês enxertadas e não enxertados em condições de ambiente protegido

The aim of this experiment was evaluate the effect of the bioestimulant administered in grafted and non-grafted japanese cucumber (Cucumis sativus L.) plants, under greenhouse conditions, in gas exchanges during the development of the plant and in the increase of yield. The experiment was carried out in the experimental area of Agronomic Sciences University of UNESP, Campus of Botucatu, São Paulo State, Brazil. The experiment design was completely randomized, in a factorial arrangement of 2x5, grafted and non-grafted plants and 5 treatments with bioestimulant: control, indolbutyric acid 0,0005% + citocinine 0,0009% + gibberelic acid 0,005% 250 mL ha-1; indolbutyric acid 0,0005% + citocinine 0,0009% + gibberelic acid 0,005% 375 mL ha-1; indolbutyric acid 0,0005% + citocinine 0,0009% + gibberelic acid 0,005% 500 mL ha-1; Yuca extract (Yucca elephantipes) + manganese + iron + copper + sulfur 375 mL ha-1 applied 15 days after the transplant, in intervals of 7 days between the applications, via leaf. The effect of the treatments were evaluated through the observations of the following characteristics: production of fruits (number and mass), average mass of the fruit and measures of gas exchanges. It can be concluded that indolbutyric acid 0,0005% + citocinine 0,0009% + gibberelic acid 0,005% 250 mL ha-1 increased CO2 assimilation rate and the water efficiency, influencing in addition to increase the quantity of the fruits

Estudo para determinação da kVp em equipamentos de raios X convencional

The quality assurance control program (QACP) in clinical radiology is very important to acquire, image quality, patient dose reduction and cost for the institution. The verification of the real tension on the x ray tube, it is one of many parameters that may be determined on a QACP. This act on image quality as absorbed dose in patient. Once proved the importance of this fact, this study come up with the determination of tension to any X ray tube used on medical routine, on quick, safe and low cost manner. To reach the aim of this study, the methodology consisted on measuring expose rates (ER) using different thicknesses of copper (Cu) plates like filters and relating these results with Half Layer Value (HLV). Afterwards, the HLV was associated to real tension that was acquired with kilo voltage of peak (kVp) measurers used on clinical routine. So walking in this path, when performed the ER, on any X ray tube, it’s possible acquire the HLV and consequently the real kVp, considering measures obtained before, on the methodology of kVp estimative development

Análises de solos e sedimentos de corrente, nas áreas das usinas de fundição de chumbo do Calabouço (CIEM-CPRM) e de beneficiamento de minério aurífero do Morro do Ouro (Parque Municipal Morro do Ouro), no município de Apiaí - SP

Located in Apiaí, Vale do Ribeira, southern region of São Paulo state, the Usina do Calabouço areas (CIEM / CPRM) and Morro do Ouro (Parque Municipal do Morro do Ouro), were targeted for stud ies due to its old buildings, respectively a foundry in lead ore, called Experimental de Chumbo e Prata (Usina Calabouço), and a gold mine with processing plant of the gold-bearing ore produced there. Nowadays, the areas are used for public visitation, and in both places the rests of the buildings remains. Particularly in the CIEM/CPRM, due to the materials witch was produced before, it can suggest the existence of anomalous amounts of the involved metals (CIEM / CPRM) and possible contaminations by chemical products used in the improvement of the auriferous ore (P. M. Morro do Ouro). These potential contamination were confirmed with geochemical survey of soils and current sediments accomplished in both areas, for the elements arsenic, cadmium, lead, copper, mercury, silver and zinc, which were used as parameters guiding values for soil and groundwater the state of São Paulo (CETESB), and watershed values stipulated by CPRM (poor, background and anomalous), in it rising geochemistry during Folha Apiaí's execution (SG-22-X-B-V).

Cinética de degradação do sistema resina epóxi/fibra de carbono

With the increasing demand for electricity, the retraining of transmission lines is necessary despite environmental restrictions and crossings in densely populated areas to build new transmission and distribution lines. Solution is reuse the existent cables, replacing the old conductor cables for new cables with higher capacity power transmission, and control of sag installed. The increasing demand for electrical power has increased the electric current on the wires and therefore, it must bear out temperatures of 150°C or more, without the risk of the increasing sag beyond the established limits. In the case of long crossings or densely populated areas, sag is due to high weight of the cable on clearance. The cable type determines the weight, sag, height and the towers dimensions, which are the items that most influence the investment of the transmission line. Hence, to reduce both cost of investment and maintenance of the line, the use of a lighter cable can reduce both number and the height of the towers, with financial return on short and long term. Therefore, in order to increase the amount of transmitted energy and reduce the number of built towers and sag, is recommended in the current work substitute the current core material (steel or aluminium) for alternatives alloys or new materials, in this case a composite, which has low density, elevated stiffness (elasticity module), thus apply the pultruded carbon fiber with epoxy resin as matrix systems and perform the study of the kinetics of degradation by thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA) and differential scanning calorimetry (DSC), according to their respective standards

Estudo da variação do limite de resistência à tração ao longo da bobina de vergalhão de alumínio 1350 AA

Therebar of aluminum 1350 AA produced by CBA are used inthe manufacture of wires and cables for electric power transmission, which marketshows increasingly favorableto aluminum due to itslow densityand high electrical conductivity, but to ensure that this materialmeets all specifications of projectsfor electricity transmission, it must have homogeneity in the chemicaland mechanicalproperties.One of the points of improvement in the process of rod production isreducing the high variation of the limitof tensile strengthalong the coils, therefore, this work seeks a better understanding of the factors that significantly influence the mechanical properties of rebar, specifically assessing the influence oftemperatureat the output of the coils, which can cause a recovery effect on the material andif thereare relevantdifferences between the two modes of rebar production: auto and manual.Samples of six coils have been specifically produced forthis study, which weresubsequently subjected to different annealing temperatures for one hour and ten minutes, similar to what occurs in the output of the coil from the machine. The tensile tests showed that aluminum 1350 AA is significantly influenced by temperature, whose behavior was very similar to that presented in the literature. It was found that the phenomenon of recovery occurred more significantly at high temperatures. Through the optical electron microscope Zeiss, 18 surface maps were made with 100x magnification for each sample in different conditions and the images were analyzed using entropy and fractal dimension, aiming to relate the condition of surface hardening on mechanical property of the samples in that condition. The results showed that these methods can be applied, provided they do not have any kind of imperfection on the surface, once they can influence the results. The study concluded that a more efficient cooling is required in ... (Complete abstract click electronic access below)

Cachaça tradicional e bidestilada: aceitação e perfis sensoriais

O mercado da cachaça, bebida tipicamente brasileira, vem crescendo muito no Brasil e no mundo. Porém, apesar do grande volume de produção, nosso país ainda exporta uma quantidade muito pequena do produto, cerca de 1%. Visando atingir o mercado internacional, e também atender às crescentes exigências do mercado interno, é ainda necessária uma melhor padronização dessa bebida, além da obtenção de um produto com boas características físico-químicas e sensoriais. O principal objetivo deste trabalho foi realizar estudos, visando avaliar o efeito do processo de bidestilação e do envelhecimento, na padronização e na qualidade sensorial, da cachaça comparando-se a aceitação de amostras de cachaça obtidas tradicionalmente, por bidestilação e também envelhecidas. Assim, foram produzidas oito amostras de cachaça, por destilação simples e utilizando-se o processo de bidestilação, em alambiques de cobre, a partir do mesmo vinho de cana, e então envelhecidas em tonéis de carvalho durante seis meses. As referidas amostras foram submetidas a testes de aceitação sensorial utilizando-se sessenta provadores e os resultados obtidos, mostraram que a bidestilação produz uma aguardente mais padronizada e ainda que sua aceitação, aumenta de forma mais acentuada durante o processo de envelhecimento

Mapeamento geológico no alvo Morro do Corcunda - Greenstone Belt Pilar de Goiás (Santa Terezinha-GO)

The following work refers to a geologic mapping in the Morro do Corcunda target, located between the cities of Pilar of Goiás and Santa Terezinha in the northwest portion of the State of Goiás. This mapping was carried through in 1:10,000 scale and covers an area of approximately 60km2. Collections of samples had been carried through for laborarorial analysis, and from those twenty-three thin scetions have been produced in order to describe the main lithologies that occur in the area. It was possible to observe anomalous gold targets in the region through chip samples carried through during the stage of field work. The gathered field data and the ones that have been made available by the company Yamana Gold Incorporation were congregated, and a data integration was carried through. This integration made possible the correlation of the litologies found in field with the Greenstone Belt Pilar de Goiás sequence and the structural evolution of the area.

Síntese por reação em estado sólido de pós cerâmicos multifuncionais à base de CaCu3Ti4O12 modificados com estrôncio

The study of ceramic materials is constantly evolving, especially in research related to advanced ceramics. Once these have many applications, this paper relates to synthesis by solid state reaction of calcium copper titanate (CCTO) ceramic material means doping with strontium. The powders were characterized using thermal analysis techniques such as TG (thermogravimetry), DTA (differencial thermal analysis), dilatometry, X-ray diffraction (XRD) and scanning electron microscopy (SEM). The compositions have submitted weight loss at around 6% with respect to carbonates used, and was attributed a temperature of 950° C to perform the calcination according to thermogravimetric analysis. After the process of calcination and milling, the particles presented approximately spherical shapes and high percentages of substitution Ca2+ with Sr2+ was evident by the presence of necks between to particles due to the milling calcination. Analyses with Energy Dispersive Spectroscopy (EDS) showed stoichiometries in different samples very similar to the theoretical stoichiometry

Mapeamento geológico e petrologia dos complexos máfico-ultramáficos de Mangabal Sul e Mangabal Norte, região de Sanclerlândia, Goiás

The Mangabal Sul and Mangabal Norte mafic-ultramafic complexes are interpreted as intrusive stratiform bodies in the Goiás Magmatic Arc during the Brazilian cycle, being economically important for harboring significant amounts of nickel and copper sulfides. The main lithotypes of the complexes are gabbronorites, olivine gabbronorites, pyroxenites and peridotites, with variated degrees of deformation, recrystallization and metamorphism superimposed, with metamorphic peak of amphibolite to granulite facies evidenced mainly by the occurrence of coronitic olivine in metamafic rocks and the occurrence of syn-kinematic retrometamorphism associated with the development of the main foliation Sn. The Sn foliation planes show NE-SW preferential direction, consistent with the foliation direction of VIII the enclosing gneisses and schists, also concordant with the general elongation of mafic and ultramafic bodies displayed on map. The sulfide phase presents textures that indicate remobilization, associated with the occurrence of significant amounts of rutile within the ore which reinforces this idea. Along with the sulfides, the occurrence of expressive quantities of titanium oxides such as ilmenite and rutile, make the area more economically attractive. It can be suggested that the Mangabal Norte and Mangabal Sul complexes are contemporary, have the same genetic affinity and suffered the same deformational and metamorphic processes, evidenced by their structural and petrological similarities

Estudo dos micronutrientes na produção de ramnolipídios por mutante de Pseudomonas aeruginosa LBI

Os biossurfactantes apresentam inúmeras vantagens, como baixa toxicidade, biodegradabilidade e alta estabilidade, mas não são amplamente utilizados devido ao custo de produção. A utilização de substratos baratos, linhagens mutantes que associados à otimização das condições de cultivo pode levar a uma redução nos custos, possibilitando assim a substituição dos surfactantes sintéticos pelos biológicos. Uma maneira empregada para maximizar a produção dos biossurfactantes é a limitação de nutrientes. Os esforços empregados nesse sentido são direcionado para as proporções carbono: nitrogênio, entretanto os efeitos dos elementos traços são pouco conhecidos. Devido a esses fatores, o presente trabalho avaliou a importância dos seguintes elementos traços: ferro, zinco, cobalto, cobre, manganês e do sal citrato de sódio dihidratado, nas fermentações realizadas utilizando o mutante de Pseudomonas aeruginosa LBI 2A1. Para tanto foram realizadas fermentações em frascos Erlenmeyer, onde se utilizou diferentes concentrações desses elementos. A influência dos mesmos na produção de ramnolipídios foi constatada, uma vez que a produção desse biotensoativo foi aumentada em mais de três vezes alterando apenas a concentração de um único elemento traço (Fe). Os experimentos realizados permitem, também, inferir a respeito das melhores concentrações desses micronutrientes para a produção de ramnolipídios