Evaluación de la posible capacidad genotóxica de fármacos antihipertensivos: losartán e irbesartán

[es] La hipertensión arterial (AHT), estado patológico definido como la elevación persistente de la presión arterial, es considerada uno de los principales problemas de salud pública. El tratamiento de la patología se realiza preferentemente con fármacos antihipertensivos. Los pacientes se encuentran sometidos a una exposición larga e ininterrumpida a estos fármacos. Los fármacos antihipertensivos se clasifican en función del mecanismo de acción por el que logran su efecto. Una de las clases son los antagonistas de los receptores de angiotensina II (ARB). Los ARB son la última clase terapéutica incluida en la terapia antihipertensiva. En este Trabajo Fin de Grado, se ha evaluado la posible capacidad genotóxica in vitro, de dos tipos de fármacos ARB (losartán e irbesartán) mediante el empleo de uno de los métodos citogenéticos más utilizados, el ensayo de micronúcleos (MN). El índice de división nuclear (NDI) fue usado también como medida de genotoxicidad. El análisis se ha realizado en linfocitos de sangre periférica (PBL) de 10 individuos control mediante dos tipos de cultivo uno sin fármaco y otro añadiendo los fármacos a los cultivos en una concentración igual a la que se encuentra en el plasma de pacientes. Los resultados muestran un aumento estadísticamente significativo de la frecuencia de células binucleadas con micronúcleos (BNMN) pero no se observan diferencias estadísticamente significativas en el índice de división nuclear. Estos resultados sugieren un posible efecto genotóxico de los fármacos pero sería necesario llevar a cabo estudios en una población más amplia e in vivo con los mismos fármacos para confirmarlo.

Surveying bacterial human pathogens in shrimp culture in Thailand

Any presence of bacterial human pathogen in shrimp products may be of public health concern. This note concludes that Salmonella do not appear to constitute a part of the microbial flora where shrimp culture is practiced in Thailand. Vibrio cholerae 01, the cause of cholera are rarely recovered from the environment with no isolates containing genes encoding cholera toxin. Further studies are needed to describe the prevalence of bacterial human pathogens in shrimp culture, especially determination of possible postharvest cross-contamnation with these pathogens

Reducing feed costs in semi-intensive finfish culture: an update on mixed feeding schedules and an idea for enhancing endogenous food supply in ponds

Some interesting ideas on improving the cost-effectiveness of feeding in semi-intensive finfish aquaculture are presented.

Community-based fish culture in seasonal floodplains

During the rainy season in extensive river floodplains and deltaic lowlands, floods render the land unavailable for crop production for several months each year. These waters are considerably underutilized in terms of managed aquatic productivity. This raises the opportunity to enclose parts of these floodwater areas to produce a crop of specifically stocked aquatic organisms aside from the naturally occurring ‘wild’ species that are traditionally fished and are not affected by the culture activity, resulting in more high-quality, nutrient-dense food production and enhanced farm income for all stakeholders, notably the poor. The WorldFish Center and its national partners recently tested the concurrent rice-fish culture in the shallower flooded areas and the alternating rice-fish culture in the deep-flooded areas of Bangladesh and Viet Nam through a community-based management system. Results indicate that community-based fish culture in rice fields can increase fish production by about 600 kg/ha/year in shallow flooded areas and up to 1.5 t/ha/year in deep-flooded areas, without a reduction in the rice yield or wild fish catch.

Fishpen culture as a new production system in dammed valleys in the mid-Casamance, Senegal

Fishpen culture is a possible means to increase fish production in Casamance, Senegal and to develop aquaculture without negative environmental effects. To study this possibility, a fishpen study was conducted in two dammed valleys, Guidir and Balobar in the area. Wild Sarotherodon melanotheron from 7.7 to 25.4 g and Tilapia guineensis from 7.7 to 35.0 g were stocked at densities ranging from 1.5 to 2.5 individuals/ super(m). Results are summarized in this article.

Production-related risk in rice-fish culture: a target MOTAD analysis

The article is a summary of the preliminary results of an AFSSRN-funded study on Risk Programming of Rice-Fish Production Systems in the Philippines conducted early 1993 by the AFSSRN-CLSU team. The results show that rice-fish culture leads to a higher rice production compared to rice monoculture.

Hatchery and culture technology for the sea cucumber Holothuria scabra Jaeger, in India

The seed of the sea cucumber Holothuria scabra jaeger is being produced at the Central Marine Fisheries Research Institute in India. This article describes the techniques being used in the production of seed and the experiments being carried out for the rearing of juveniles. Trials to grow juveniles in hatcheries on prawn farms have shown spectabular results that are both cost efficient and environmentally friendly.

Freshwater pearl culture in India

Freshwater pearl culture is growing as a source of employment and income in many Southeast Asian countries. Bangladesh, Thailand, the Philippines and Vietnam have initiated freshwater pearl culture projects in recent years. In India, the Central Institute of Freshwater Aquaculture is carrying out research on identification of suitable local pearl mussel species (Genera Lamellidens)defining appropriate surgical implantation procedures, developing post-operative care procedures and captive pond culture of mussels.

Integration of freshwater prawn culture with rice farming in Kuttanad, India

The integration of paddy cultivation with prawn/fish culture can become a viable alternative to effectively utilize the vast area of derelict polders (embanked coastal flood plains) in Kuttanad, India. Nearly 55 000 ha of wetlands in Kuttanad are available for paddy cultivation year-round. Around 5 000 ha of the polders are utilized for Macrobrachium rosenbergii culture as a follow-up crop. Of the total area, about 250 ha of fallow polders are utilized for monoculture of M. rosenbergii from March to October, while in 4 750 ha polyculture with Indian and exotic carps is practiced from November to June. Stocking density is 15 000 to 60 000/ha for monoculture of M. rosenbergii, while in polyculture with carps, it is 5 000 to 20 000/ha of prawn and 5 000 to 10 000/ha of fish. Production from monoculture varies from 95 to 1 297 kg/ha whereas production from polyculture systems it is 70 to 500 kg/ha of prawn and 200 - 1 200 kg/ha of fish. Profits range from Rs. 5 000 to 20 000/ha. An evaluation is made of how the present polders of Kuttanad are best utilized for culture of M. rosenbergii following different systems of integrated farming and how the integration is useful in the aquaculture sustainability of Kuttanad, a tropical wetland ecosystem.

Safe use of pesticide in rice-fish culture

Results of the study on the relative safety of nine different pesticides: Diazinon 40% EC, Diazinon 10 G, Elsan 50% EC, Sumithion 50% EC, Furadan 3 G, Padan 50% SP, Kitazin 48% EC, Kitasin 17% G and EPN 45% EC for various fish species: Cyprinus carpio, Labeo rohita, Oreochromis mossambicus, Channa punctata, Clarias batrachus and Anabas testudineus used in rice-fish culture are presented and discussed.

Mangrove forests and shrimp culture in Ngoc Hien District, Minh Hai Province, Vietnam

As a result of increased population growth, the attraction of high profits from shrimp culture and ineffective mangrove forest management, a number of serious environmental problems exist in Ngoc Hien District, Minh Hai Province, Vietnam. These environmental problems are highlighted in this article.

A simple and objective bioeconomic index for choosing species for culture

Details are given of the development of a bioeconomic culture index that may be used to assist in choosing fish for culture. This index takes into account growth performance indexes and mean annual prices of the fish. Examples are given of the culture indices of some of Kuwait's commercially important fish species. The proposed indices given realistic output for these fish and allow comparison and evaluation of different taxa with widely differing biological and economic characteristics.

Pen fish culture in reservoirs: an alternative to land based nurseries

An experiment to rear carp seed was conducted in Tamil Nadu, India during October 2001 to April 2002 as a part of an ambitious programme aimed at standardization of pen fish rearing technology for production of stocking material of desired size at a lower cost. The experiment used six pens erected using locally available materials in the exposed marginal area of an existing reservoir. The high survival rate of carps (67.2-94.7%) and reasonable returns on investment (26.2%) obtained in the experiment indicated that fish seed rearing in pens erected in suitable areas of existing reservoirs could serve as a cheaper alternative to the expensive land-based nursery ponds.