柑桔小实蝇生物学特性及其防治研究

柑桔小实蝇 Dacus (bactrocera) dorsalis (Hendel)在云南景谷1年发生5代,以老熟幼虫和蛹在土壤中越冬或越夏。2月中至8月中旬,成虫产卵于芒果果实内,致使芒果腐烂。室内用40%氧化乐果、50%杀螟松和80%的敌敌畏1 000、1 500倍液,对成虫的毒杀率达100%,幼虫达88.73%,蛹为10.0%。大田防治用1 000倍液,同时用性信息素进行诱杀,保果效果达95.74%。

Nutrient salts in the United Arab Emirates waters (the Arabian Gulf and the Gulf of Oman)

This paper deals with the levels and distributions of nutrient salts in the United Arab Emirates waters. Water samples were collected bimonthly during 1994-1995 from the marine environment of the United Arab Emirates, which extends more than 800km along the Arabian Gulf and the Gulf of Oman. Concentrations of ammonium, nitrite, nitrate, phosphate, silicate, as well as total concentrations of total dissolved nitrogen, phosphorus, and silicon in the area were scattered in the ranges: (ND-6.32; mean: 0.84 µg-at N/l), ND-3.02; mean: 0.42 µg-at N/l), (ND-10.88; mean: 1.18 µg-at N/1), (ND-4.22; mean: 0.62 µg-at P/l), (1.14-28.80; mean: 6.52 µg-at Si/l), (1.52-39.58; mean: 12.28 µg-at N/l), (0.40-4.98; mean: 1.07 µg-at P/l), and (2.77-44.74; mean: 13.02 Si/l) respectively. Of inorganic nitrogen species, ammonium was the highest in the Arabian Gulf waters and nitrate was the highest at the Gulf of Oman. The dissolved inorganic nitrogen total species, phosphate and silicate amounted to 16.4, 47.6, 56.5% respectively, of the concentrations of nitrogen, phosphorus and silicon in the Arabian Gulf and 22.6, 64.4, 44.9% respectively, in the Gulf of Oman, indicating that more than 80% of nitrogen was present in organic forms. Distributions of nutrient in the two regions were higher in the summer season and lower in the winter season due to the oxidation of organic materials. Regional distributions revealed higher values for nitrite (1.3 times), nitrate (2.8 times), phosphate (2.2 times), total dissolved nitrogen (1.3 times), total dissolved phosphorus (1.6 times), and total dissolved silicon (1.3 times) in the Gulf of Oman compared to the Arabian Gulf, indicating more oligotrophic conditions at the Arabian Gulf Whereas no distinct patterns of distribution were observed in the Arabian Gulf waters, an increase in the seaward direction was measured at the Gulf of Oman. Vertical distributions indicated a general increase with depth in the two regions. The mean ratios for total concentrations of phosphorus, nitrogen, and silicon in the Arabian Gulf (1: 11.6: 12.6) and the Gulf of Oman (1: 10.1: 11.8) were lower than the Redfield ratio.

Assessment of the heavy metals among suspended particulates and dissolved phases in Suez Canal water

The concentrations and distribution of particulate and dissolved heavy metals, viz: Cu, Zn, Pb, Cd, Fe and Mn have been determined seasonally during 2003 in water samples collected from the Suez Canal. The presented data clarifies that the metals exhibited clear differences in their distribution between particulate and dissolved forms. The concentration of particulate heavy metals ranged between 0.09-3.13, 0.57-15.02, 0.18-3.87, 0.02-0.73, 2.74-49.62 and 0.15-5.08 µg/L for Cu, Zn, Pb, Cd, Fe and Mn, respectively. In the same respect, these values for dissolved forms were 0.28-4.12, 0.57-9.08, 0.27-2.50, 0.02-1.24, 1.94-42.50 and 0.11-3.65 µg/L. The concentrations of particulate metals viz: Zn, Pb, Cd, Fe and Mn were high was compared to the dissolved forms. Dissolved copper, rather than particulate, showed the highest percentage of total copper. The particulate forms of Pb, Cd, Fe and Mn always had higher concentrations than the dissolved forms during the course of study except in the summer season. The northern part of the Suez Canal at Port Said showed mean concentrations of particulate and dissolved Cu=1.43 and 2.10, Zn=8.61 and 3.17, Pb=1.72 and 1.23, Cd=0.35 and 0.35, Fe= 23.49 and 15.83 and Mn=2.09 and 1.82 µg/L. These high concentrations may be attributed to the greater activities, particularly loading and unloading operations at Port Said harbour s industrial effluents and domestic drainage of Port Said city. In contrast, the Sinai side could be considered as reference site, as it was almost clean, i.e., without harmful outfalls, where Cu=0.16 and 0.56, Zn=2.14 and 0.94, Pb=6.29 and 3.44, Cd=0.055 and 0.088, Fe=6.29 and 3.44 and Mn=0.56 and 0.26 µg/L for particulate and dissolved metals respectively.

脂肪酸钙对放牧肉牛超数排卵效果及雌二醇和孕酮水平的影响

以探讨添加脂肪酸钙对放牧肉牛(婆罗门和BMY)超数排卵效果及血中雌二醇(E2)和孕酮(P4)水平的影响为目的.将48头供体分为Ⅰ(放牧或对照组)、Ⅱ(放牧+浓缩饲料500 g/d)和Ⅲ(放牧+浓缩饲料500 g/d+脂肪酸钙100 g/d)3组,从同期发情处理开始到冲胚结束试验期32 d,并对供体牛在8个处理时段进行采血,用放射免疫分析法(RIA)分析E2和P4水平.试验结果表明:Ⅰ,Ⅱ,Ⅲ组供体牛超数排卵的排卵率分别为75.4%,74.4%和81.7%(P＞0.05),受精率分别为81.4%,55.6%和93.2%(P＞0.05);获胚(卵)数分别为7.0枚,6.3枚和7.3枚(P＞0.05),可用胚数分别为3.9枚,2.6枚和4.7枚,Ⅲ组显著高于Ⅱ组(P＜0.05);E2平均水平分别为0.57 pg/mL,0.47 pg/mL和0.63 pg/mL(P＞0.05),P4平均水平分别为3.53 ng/mL,5.03 ng/mL和3.74 ng/mL,Ⅱ组高于Ⅰ,Ⅲ组(P＜0.01).表明适量添加脂肪酸钙能改善全放牧肉牛的营养、增强体质,促进排卵与受精,增加超数排卵的可用胚数;能使供体牛的E2和P4水平比对照组有所提高;添加效果冬、春季优于夏、秋季.

Report of the training workshop on the application of Ecopath with Ecosim to BOBLME, Phuket, Thailand, 8-12 September, 2014

The workshop's objective was to provide information to improve a regional Ecopath with Ecoism (EwE) model.The workshop described the basis and principles underlying the software, encompassing the basics of Ecopath to Ecospace, economics and management strategy evaluation.

Influence of cold storage time on the protein changes and fatty acids deterioration of (Rutilus frisi kutum) during frozen storage

The main aim of this research was to identify fatty acids composition of Caspian sea of White fish Rutilus frisi kutum tissue and their changes during one year cold storage (-18Ċ).The secondary aim was to determine the changes of moisture, ash, protein, fat, and to investigate the effects of storage time on peroxide, TBAi, FFA, and extractability of myofibrillar proteins of the fish tissue during one year cold storage (-18 Ċ). 10 samples of (Rutilus frisi kutum) were randomly collected from Anzali landings. The samples were frozen at -30 Ċ and kept in cold storage at -18Ċ for one year. According to time table, the samples were examined. The results showed that 27 fatty acids were identified. The unsaturated fatty acids (UFA) and saturated fatty acids (SFA) were 74/09 and 21/63 %, respectively, in fresh tissue. So that DHA (C22:6) oleic acid (C18:1c) had high amounts (15/07 ,20/57 ) among the UFA and palmitic acid (C16:0) was the most (13/09 %) among the SFA. The effects of freezing and cold storage on fish tissue showed that UFA and SFA contents have reached to 58/79 and 22/17 %, respectively, at the end of cold storage. It indicated that these compound change to each other during frozen storage. Also ω-3 and ω-6 series of fatty acids was 24/22 and 15/56% in fresh tissue, but their contents decreased to 8/68 and 5/11% at the end of period. Among the fatty acids C22:6, C18:1c and C16:0 had the most changes. The changes of fatty acids were significantly at 95% level expected for C18:0. Results showed that moisture, ash, protein, and fat contents were 75/9±0/03, 1/28±0/012, 21/8±0/2, and 4/1±0/01 % respectively, in fresh tissue. The moisture, ash, protein, and fat contents were 72/3±0/04, 1/83±0/05, 1/91±0/01 and 19/9±0/01 % respectively, at the end of storage period. Lipid damage was measured on the basis of free fatty acids (FFA), peroxide value (PV), and Thiobarbituric acid index (TBA-i). PV, TBARS and FFA concentration of frozen Caspian Sea white fish stored at -18 Ċ the temporal variation of these three variables were statistically significant (p<0.001). Results of White fish myofibrillar proteins showed aggregation of bound reduced for stored at 12 months. SDS-PAGE analysis revealed that, the intensity of the myosin heavy chain and actin bound was reduced with increasing storage time. SDS-PAGE patterns showed that myosin heavy chain was much more susceptible to hydrolysis than actin. Key words: Rutilus frisi kutum, frozen storage, ω-3, ω-6, protein myofibrillar

Identification and measurement of fatty acids, amino acids in roe of tuna fish (Thunnus tonggol) and its TVB and peroxide value during cold storage

The first aim of this research was to identify fatty acids, amino acids composition of Thunnus tonggol roe and their changes during cold storage (-18'C). The second aim was to determine the changes of moisture, protein, fat and ash contents of the roe during one year cold storage (-18'C). 60 samples of longtail tuna (Thunnus tonggol) ovaries were randomly collected form Bandar-e-Abbas landings. The samples were frozen at-30'C and kept in cold store at -18'C for one year. According to a time table, the samples were examined for identification of fatty acids, amino acids, moisture, protein, fat, ash, peroxide and T.V.N. and their changes were evaluated during this time. The results showed that 26 fatty acids were identified. The unsaturated fatty acids (UFA) and saturated fatty acids (SFA) were 62.33 and 37.6%, respectively, in fresh roe. So that, DHA (C22:6) and oleic acid (C18:1) had high amounts (24.79 and 21.88%) among the UFA and palmitic acid (C16:0) was the most content (22.75%) among the SFA. The PUFA/SFA was 0.91. Also, 17 amino acids were identified that essential amino acids (EAA) and nonessential amino acids (NE) were 10478 and 7562 mg/100g, respectively, and E/NE was 1.38. Among the EAA and NE, lysine (2110mg/100g) and aspartic acid (1924 mg/100g) were the most contents. Also, results showed that moisture, ash, protein and fat contents were 72.74, 1.8, 19.88 and 4.53%, respectively, in fresh roe. The effects of freezing and cold storage on the roes showed that UFA and SFA contents have reached to 49.83 and 48.07%, respectively, at the end of cold storage. It indicated that these compounds change to each other during frozen storage. Also, n-3 and n-6 series of fatty acids were 32.75 and 1.61% in fresh roe. But their contents decreased to 22.96 and 1.25% at the end of period. Among the fatty acids, 22:6 and C16:0 had the most changes. The changes of fatty acids were significantly at 95% level except for C15:1, C18:3(n-3) and C20:4(n-6). All of the amino acids decreased in frozen storage and their changes were significantly (P<0.05). EAA was 7818 mg/100g and E/NE was 1.27 at the end of storage period. Among the amino acids, leucine and lysine had the most changes. Moisture, ash, protein and fat contents were 70.13, 1.82, 19.4 and 6.51%, respectively, at the end of storage period. The peroxide value and T.V.N. increased during storage. So that, their contents have reached to 5.86 mg/kg and 26.37 mg/100 g, respectively, at the end of frozen storage. The best shelf life of Thunnus tonggol roe was 6 or 7 months, because of lipid oxidation and increasing of peroxide.

云南8种树蛙的染色体组型比较研究

报道了云南8种树蛙的染色体组型, 并对目前所已知的树蛙种类的染色体组型进行了比较。

八种稀土金属配合物和铂配合物抗HIV 活性的研究

　为了寻找治疗艾滋病的新药,研究了8 种稀土金属(镧、铒、镝、钆、镨、镱、钐、钕) 配合物和铂 配合物的抗HIV 活性。我们以C8166 细胞系为指示细胞,测定样品对HIV - 1IIIB诱导的细胞合胞体形成 的抑制作用,并以MTT方法测定它们对细胞存活率的影响,以研究稀土和铂配合物对细胞的毒性。根 据公式,计算出其CC50和EC50值,并进一步计算出其TI 值。结果表明,镧配合物、钐配合物和铂配合物 的EC50值分别为0. 006μg/ ml 、74. 28μg/ ml 和21. 54μg/ ml ,它们的治疗指数分别为63、21 和45 ,具有一定的 抗HIV - 1 活性。其它的配合物具有很低或者不具有抗HIV - 1 活性。

Sequence context analysis of 8.2 million single nucleotide polymorphisms in the human genome

We analyzed n-mers (n=3-8) in the local environment of 8,249,446 human SNPs and compared their distribution with that in the genome reference sequences. The results revealed that the short sequences, which contained at least one CpG dinucleotide, occurred