New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK)

4-Methyl-5-beta-hydroxyethylthiazole kinase (ThiK) catalyses the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole. This work reports the first crystal structure of an archaeal ThiK: that from Pyrococcus horikoshii OT3 (PhThiK) at 1.85 angstrom resolution with a phosphate ion occupying the position of the beta-phosphate of the nucleotide. The topology of this enzyme shows the typical ribokinase fold of an alpha/beta protein. The overall structure of PhThiK is similar to those of Bacillus subtilis ThiK (BsThiK) and Enterococcus faecalis V583 ThiK (EfThiK). Sequence analysis of ThiK enzymes from various sources indicated that three-quarters of the residues involved in interfacial regions are conserved. It also revealed that the amino-acid residues in the nucleotide-binding, magnesium ion-binding and substrate-binding sites are conserved. Binding of the nucleotide and substrate to the ThiK enzyme do not influence the quaternary association (trimer) as revealed by the crystal structure of PhThiK.

Orientational Order of Liquid-Crystalline 4-Alkyl-N-(p-cyanophenyl)piperidines by 2H and 13C NMR Spectroscopies

The orientational order of nematic 4-alkyl-N-(4-cyanophenyl) piperidines (I) has been determined from H-2 and C-13 NMR spectra. Molecular-order parameters are derived from the carbon-13 chemical shift of the cyano carbon atom in the nematic and the isotropic phases; the sign of the diamagnetic anisotropy is positive. Deuterium quadrupolar splittings from the partially deuterated piperidine ring of I are then related to various C-D bonds.

Role of the loop L-4,L-5 in allosteric regulation in mtHsp70s: in vivo significance of domain communication and its implications in protein translocation

Mitochondrial Hsp70 (mtHsp70) is essential for a vast repertoire of functions, including protein import, and requires effective interdomain communication for efficient partner-protein interactions. However, the in vivo functional significance of allosteric regulation in eukaryotes is poorly defined. Using integrated biochemical and yeast genetic approaches, we provide compelling evidence that a conserved substrate-binding domain (SBD) loop, L-4,L-5, plays a critical role in allosteric communication governing mtHsp70 chaperone functions across species. In yeast, a temperature-sensitive L-4,L-5 mutation (E467A) disrupts bidirectional domain communication, leading to compromised protein import and mitochondrial function. Loop L-4,L-5 functions synergistically with the linker in modulating the allosteric interface and conformational transitions between SBD and the nucleotide-binding domain (NBD), thus regulating interdomain communication. Second-site intragenic suppressors of E467A isolated within the SBD suppress domain communication defects by conformationally altering the allosteric interface, thereby restoring import and growth phenotypes. Strikingly, the suppressor mutations highlight that restoration of communication from NBD to SBD alone is the minimum essential requirement for effective in vivo function when primed at higher basal ATPase activity, mimicking the J-protein-bound state. Together these findings provide the first mechanistic insights into critical regions within the SBD of mtHsp70s regulating interdomain communication, thus highlighting its importance in protein translocation and mitochondrial biogenesis.

Implicações da quebra de ética e decoro parlamentar na 4ª e 5ª legislaturas da Câmara Legislativa do Distrito Federal

Aborda as implicações da quebra de ética e decoro parlamentar na 4ª e 5ª Legislaturas da Câmara Legislativa do Distrito Federal, os critérios técnicos e políticos utilizados na condução e julgamento dos processos por quebra de decoro e confirma a tese de que tais processos, de caráter político, passam pela lógica do corporativismo. Para fundamentar a análise, faz uma breve retrospectiva bibliográfica e examina os diversos processos relativos à quebra de decoro parlamentar na 4ª e 5ª Legislaturas, à luz da Resolução n° 110, de 17 de maio de 1996, que instituiu o Código de Ética e Decoro Parlamentar e criou a Comissão de Ética, e suas alterações posteriores. Analisa as posições dos parlamentares nos casos de quebra de princípios éticos e de decoro no âmbito da Comissão de Ética e Decoro e da Corregedoria, ao longo das duas últimas legislaturas concluídas.

Heriotzaren trataera haur hezkuntzan: 4 eta 5 urteko geletarako proposamen didaktikoa

128 p. : il.

Aquisição social onerosa e privada da propriedade: artigo 1228, 4 e 5 do código civil

A pesquisa proposta pretende esclarecer os pontos obscuros e controvertidos do artigo 1228, 4 e 5 do Código Civil, tendo por finalidade a busca da efetividade de tal dispositivo legal, que possui, na sua essência, o reconhecimento do direito fundamental de moradia e, ainda, tutela o direito ao trabalho. O dispositivo em questão rompe com o paradigma da posse como mera sentinela avançada do direito de propriedade e reconhece a defesa da posse autônoma exercida por aqueles que realmente cumprem com a sua função social. A partir do preenchimento dos requisitos previstos na lei, concede-se a legitimação da posse aos possuidores e, com o pagamento da indenização ao proprietário, converte-se a posse em direito de propriedade. Dessa forma, o instituto visa não apenas à regularização fundiária de áreas urbanas ou rurais, mas, principalmente, à efetividade dos direitos fundamentais de moradia e trabalho, que dão substância ao principio norteador de qualquer sociedade civilizada: o princípio da dignidade da pessoa humana. Assim sendo, na busca pela efetividade do dispositivo, o estudo tem ainda como finalidade desenvolver a natureza jurídica específica do instituto, reconhecendo-o como um modo autônomo de aquisição onerosa do direito de propriedade, não se equiparando a formas de desapropriação ou de usucapião do direito de propriedade.

放牧对内蒙古冷蒿小禾草草原植物群落特征的影响

了解天然草原放牧生态系统中，不同管理措施对草－畜系统的影响及系统中草－畜互作方式，是实现草原生态系统持续管理的基础。在内蒙古典型草原区，我们建立了24hm2围栏放牧实验样地。本文通过对试验样地5个放牧率（1.33，2.67，4.00，5.33和6.67羊／hm2）和两种放牧方式下（连续放牧与轮牧）一年排除放牧后植被变化的研究，试图揭示8年的放牧对草地群落的作用结果，探寻放牧率与植物群落生产力、种类组成和演替方向的关系，以求为建立合理的放牧制度提供佐证。 研究发现：①不同的放牧率下8年的放牧使地上生物量出现显著差异，中度放牧（SR3）下的草原群落达到了最大地上生物量，在较轻的放牧率（SR1，SR2）下有超补偿生长出现，地下生物量随着放牧率的升高而下降。②放牧使群落植物组成发生重大变化，随着放牧率的升高，表现出明显的向星毛委陵菜退化草原演替的趋势，无牧的群落表现出恢复演替的趋势。③在轻牧（SR1）处理下植物群落达到了最高的多样性，在多样性与生物量之间，不能发现较具规律性的关系。④轮牧群落的生产力潜力显著高于连续放牧，连续放牧方式造成植物斑块状分布，降低了草场的利用效率。⑤放牧造成了草原植物生态位分化。

人脑星形细胞瘤PTEN/MMAC1基因突变的研究

】目的了解在人脑星形细胞瘤中678’9::;! 基因第五、第八外显子的突变情况及其在肿瘤恶性进展中的作用。 方法应用聚合酶链反应（6!<），银染6!<=单链构象多态性（>>!6）技术对?0 例人脑星形细胞瘤，@ 例正常脑组织和2 例外周 血标本进行检测。结果?0 例病人中4 例发生678’9::;! 第五、第八外显子点突变，突变率为5?ABC（4 9 ?0），其中01 例病理 !D"级的星形细胞瘤无突变，20 例#D$级的星形细胞瘤中有4 例发生点突变，突变率0?C（4 9 20），两者相较，相差显著（!E 1A1?）。结论人脑星形细胞瘤中存在678’9::;! 基因第五、第八外显子点突变。这可能是肿瘤恶性进展的重要事件之一。

江豚的年龄鉴定、生长和生殖的研究

本文根据在长江中下游,长江口和中国东北沿海收到的68头江豚标本,对江豚年龄鉴定的方法作了摸索和研究。从年龄,生长和生殖的角度系统分析了江豚的一些种群生物学特征。江豚年龄与生长的关系在一定范围内遵循的幂函数型式。4—5龄以前,江豚的生长迅速,此后减慢。江豚首次性成熟年龄雌性4龄,雄性4.5龄。性成熟时体长雌性133cm,雄性140cm。根据16头胎儿资料,用对数分割法反推出江豚的交配期较长,并有两个可能的交配高峰,分娩的高峰为2—4月。断奶年龄约为0.5龄。粗略估算长江江豚的年生殖率为20％。长江的江豚和中

长白山阔叶红松林早春植物群落特征研究

研究了长白山阔叶红松林内早春植物群落特征 ,为进一步深入研究其在森林生态系统中物质循环和能量流动等生态功能奠定基础。早春植物主要有12种2变种 ,隶属于8属4科。按Raunkiaer生活型分类标准统计 ,早春植物都属于地下芽植物。分别以相对多度、相对频度、相对显著度和重要值为标准对各种早春植物进行排序 ,重要值排在前5位的植物分别是黑水银莲花、朝鲜银莲花、荷青花、五福花、大人字果。早春植物群落物种多样性的Shannon指数为2.5229,群落均匀度为0.9560,生态优势度为0.3212。

BLENDS OF POLY[3,3-BIS(CHLOROMETHYL)OXETANE] WITH POLY(VINYL ACETATE) .1. THERMAL AND MECHANICAL-PROPERTIES

Blends of poly[3,3-bis(chloromethyl)oxetane] (Penton) with poly(vinyl acetate) were prepared. Compatibility, morphology, thermal behavior, and mechanical properties of blends with various compositions were studied using differential scanning calorimetry (DSC), dynamic mechanical measurements (DMA), tensile tests, and scanning electron microscopy (SEM). DMA study showed that the blends have two glass transition temperatures (T(g)). The T(g) of the PVAc rich phase shifts significantly to lower temperatures with increasing Penton content, suggesting that a considerable amount of Penton dissolves in the PVAc rich phase, but that the Penton rich phase contains little PVAc. The Penton/PVAc blends are partially compatible. DSC results suggest that PVAc can act as a beta-nucleator for Penton in the blend. Marked negative deviations from simple additivity were observed for the tensile strength at break over the entire composition range. The Young's modulus curve appeared to be S-shaped, implying that the blends are heterogeneous and have a two-phase structure. This was confirmed by SEM observations.

Cultivar de trigo BRS Angico testada e indicada para as regiões de adaptação 4 e 5 do Estado de Santa Catarina.

2005