Avaliação laboratorial do uso de solução salina hipertônica e isotônica e de furosemida no tratamento da intoxicação por amônia em bovinos

Para testar a eficiência de vários tratamentos de intoxicação por amônia em bovinos, foram utilizados 25 garrotes que receberam cloreto de amônio por infusão intravenosa (iv) até o surgimento de quadro convulsivo. em seguida, os animais foram alocados em um dos cinco grupos experimentais e tratados da seguinte forma: 1) controle: infusão (iv) de 300mL de solução salina isotônica (SSI), no decorrer de 4h; 2) infusão (iv) de 30mL kg-1 PV de SSI no decorrer de 4h e administração de 4L de água intraruminal por meio de sonda esofágica (ASE); 3) mesmo tratamento do grupo 2 e dose única (iv) de furosemida (2mg kg-1 PV) (F); 4) injeção (iv) de 5mL kg-1 PV de solução salina hipertônica (SSH) 7,2% nos primeiros 30min, seguida de 20mL kg-1 PV de SSI e 4L de ASE; 5) mesmo tratamento do grupo 4 e dose única de F. No decorrer de 4h após a convulsão, foram determinados os teores plasmáticos de amônia e glicose, ureia, creatinina, potássio e sódio séricos, volume e gravidade específica da urina, e excreção urinária de amônio e ureia. No momento da convulsão, os teores de amônia plasmáticos foram muito altos e idênticos em todos os tratamentos, mas no 120°min, nos grupos tratados com associação de SSH+SSI+ASE (grupos 4 e 5), houve diminuição desse metabólito. O uso de furosemida (grupos 3 e 5) não aumentou a excreção total de urina. A terapia com associação de SSH+SSI+ASE aumentou ainda o volume urinário e a excreção percentual urinária de ureia e amônia durante o período crítico da 1ª hora de tratamento, mas o uso de SSI+ASE (grupos 2 e 3) teve resultados intermediários. A eficiência do tratamento com SSH+SSI+ASE ou SSI+ASE foi superior ao grupo controle. Embora com efeito menor que o observado com SSH+SSI+ASE, a SSI+ASE promoveu melhora no quadro clínico geral e, ao término do experimento, promoveu também uma adequada desintoxicação da amônia.

ISOENZYMATIC POLYMORPHISM and ACTIVITY of PEROXIDASES of COMMON BEAN(Phaseolus vulgaris L.) UNDER SALINE STRESS

Uma das utilizações da técnica de cultura de tecidos para o melhoramento vegetal é a identificação de linhas de células que apresentem tolerância ao estresse salino. Para se estudar os mecanismos bioquímicos envolvidos na expressão genética da tolerância a salinidade, calos oriundos de eixos embrionários de quatro cultivares de feijão (Phaseolus vulgaris L.; cultivares IAC - carioca, IAPAR 14, JALO-EEP 558, BAT - 93), foram cultivados em meio sólido Murashige & Skoog (1962), suplementado com NaCl nas concentrações de 0, 20, 40, 60 e 80 mM. Após 14 dias de incubação, os calos foram coletados e analisados quanto aos padrões isoenzimáticos e de atividade das peroxidases. Os cultivares BAT e IAPAR apresentaram duas zonas de atividade em comum na região anódica e apenas uma zona enzimática específica a cada um deles (migração mais rápida).Possivelmente as duas zonas anódicas intermediárias sejam produtos do mesmo loco enzimático, porém com alelos diferentes, consequentemente diferentes mobilidades eletroforéticas. O cv. JALO apresentou duas zonas anódicas de atividade em comum com os cultivares IAC e IAPAR com uma zona anódica exclusiva de migração mais lenta, a qual apresentou atividade mais intensa de todos os cultivares analisados. Este cultivar revelou ainda uma zona catódica provavelmente dimérica e heterozigota nos indivíduos de todos os tratamentos aplicados. Provavelmente, esta é a mesma zona que ocorre em homozigose com fixação do alelo lento para os indivíduos de todos os tratamentos efetuados nos cultivares BAT e IAPAR. O cv. IAC apresentou duas bandas anódicas em comum com os cv. IAPAR e JALO. Apresentou também a banda anódica mais rápida em comum com o cv. IAPAR e uma banda anódica exclusiva de migração mais lenta. Curiosamente, os indivíduos deste cv. mantidos em meio suplementado com 20 mM de NaCl não apresentaram atividade nas três zonas anódicas mais lentas. Ocorreu no cv. IAC uma única zona de atividade catódica, dimérica e heterozigota para os indivíduos provenientes de todos os tratamentos, composta provavelmente de dois alelos diferentes da zona correspondente ao cv. JALO. Amostras provenientes dos tratamentos 40 e 60 mM de NaCl, desta zona catódica, apresentaram maior atividade enzimática. A análise da atividade da peroxidase no extrato bruto, revelou que os cultivares responderam diferentemente ao aumento da concentração salina no meio de cultura, com aumento pronunciado dessa atividade nos cultivares IAC e JALO.

Tolerância de diferentes cultivares de cana-de-açúcar (Saccharum spp.) a herbicidas

This work aimed to evaluate the tolerance of sugarcane cultivars to sulfentrazone, imazapic, isoxaflutole, clomazone and ametryn + trifloxysulfuron-sodium. The experiment was arranged in a randomized block design in a split-plot scheme. The cultivars were allocated to the plots and the herbicides to the sub-plots (five 8.0 m long rows and 1.5 m spacing, with 4 repetitions. The herbicides sulfentrazone (0.8 kg ha(-1)), imazapic (0.147 kg ha(-1)), isoxaflutole (0.1125 kg ha(-1)), clomazone (1.1 kg ha(-1)), ametryn (1.463 kg ha(-1)) + trifloxysulfuron sodium (0.037 kg ha-1) and control were evaluated on 3-yr-old ratoons of the cultivars IACSP94-2094, IACSP94-2101, IACSP93-3046, IACSP94-4004, IAC86-2480 and RB72454 in post emergence. The traits evaluated were: plant toxicity symptoms in the plant leaves; total chlorophyll content and photochemical efficiency (Fv/Fm) at 15, 30 and 60 days after application (DAA); height (cm) at 30 and 270 DAA, and plant stand (stalk m(-1)) at 30 and 180 DAA. Diameter (cm), estimated productivity (t ha(-1)) and quality analysis were evaluated at 270 DAA. The sugarcane cultivars IACSP94-2094, IACSP93-3046, IACSP94-4004, IAC86-2480, RB72454, and IACSP94-2101 especially, were susceptible to clomazone up to 30 DAA, due to leaf chlorosis and lower chlorophyll content, but had no effect on quality characteristics and productivity. The cultivars were also tolerant to other herbicides.

Tolerância de perdas de solo na bacia do rio Itiquira, Mato Grosso, Brasil

The geographic area of the present study corresponds to the basin of the Itiquira river high course (Figure 1), in the portion that extends from the tributaries of its source, in the east of the plateau Correntes/Itiquira, in the neighbourhoods the city of Alto Garç as, to the scarp of the São Jerônimo mountain range, toward the west of Itiquira, in the state of Mato Grosso, totalizing 5,361 km 2. The area is placed in the eastern part of the Alto Paraguai basin, in the western portion of the Paraná Sedimentary Basin plateau. Through GIS techniques, it was possible to asses total soil losses from the Itiquira river basin, considering the years of 1966, 1985 and 1996, being based on the Universal Soil Loss Equation. Thus, in 1966 the basin lost 201,546.94 ton of soil, with an average loss of 0.37 ton/ha/year. Considering that the total area of the Itiquira river basin is of 536,100 ha, while in 1985 the soil losses had passed to 1,760,833.40 ton, with an increase of approximately 8.5 times. The average of soil losses in 1985 was of 3.28 ton/ha/year. In 1996 the basin lost 1,662,043.24 ton, with a reduction of only 9.4% in relation to 1985 but, in relation the 1966, the increase continued in the order of 8 times. The average losses per hectare in this year was in the order of 3.10 ton/ha/year (Chart 1). The map of potential of the laminar erosion for 1966 (Figure 3), shows to the highest values in small areas, situated in the northeast of the area, in Alto Garças, with values between 10 the 20 ton/ha/year and some spots in sources of the Itiquira and Ariranha rivers, with values between 1 the 5 ton/ha/year. In a general way, however, the area presents low soil loss for laminar erosion in this year, with inferior values to 1 ton/ha/year. The higher class of erosion, over 10 ton/ha/year, occupied 2,947 ha in 1966. In the year of 1985 (Figure 4), the erosive process spread over the entire studied area, and the class of erosion over of 10 ton/ha/year, already started to occupy 78,437 ha, implying an increase of approximately 27 times in 19 years. A strong increment in the erosive process was noticed in the western part of the area, to along the BR-163 road, exactly where great areas of natural vegetation (open pasture) had been transformed in culture and pasture areas. In the north-eastern part of the area it was also noticed an increment in the erosive process in agreement with the increase of culture areas and reduction of the natural areas, but it was not of so intense form as in the western portion of the area. In the year of 1996 (Figure 5), the class of erosion over of 10 ton/ha/year had diminished for a total of 53.499 ha noticing a retraction of the erosive process in the western part of the area, alongside the BR-163 road. On the other hand, it occurred a strong increment in the northern part of the area, in the neighbourhoods of the city of Alto Garças, alongside the BR-364 road and part of the MT-040 road. In a general way, in the outskirts of the city of Itiquira, in the central part of the area, it was verified an increase of the amount of zones with erosion between 0-1 ton/ha/year, passing to the immediately superior class, of 1-3 ton/ha/year; scarce data of hydrosedimentology in the UHE Itiquira (1999), shows good agreement with the values gotten for the EUPS (Chart 2). Based on the hydric classification proposal for FAO (1967) (Chart 3), it is noticed that areas with high degree of erosion (> 50) in the analysed area are very restricted, occupying 493 ha in 1985 and 332 ha in 1996 (Chart 4). In 1996 appeared as isolated spots in the north of Itiquira and Alto Garças, however beyond limits of the Itiquira river basin. These areas require special cares in its use as agricultural areas. Aiming at identifying and indicating the areas of potential risk of erosion and that need implementation of conservation practices, it was elaborated the map of limit of tolerance to the soil losses. In 1966 (Figure 6), areas with soil losses over of the tolerable were restricted to small spots located in the eastern part of the area, occupying 0.43% of the total of the area; already in 1985 (Figure 7), this percentage passed to 5.86%, spreading for all the area; in 1996 (Figure 8) it is observed a fast reduction of the areas with soil losses over of the tolerance limit, passing 5.43% of the total of the area. All the areas with losses over of the tolerable value must be considered as risk areas and were done in these areas studies for implementation of conservation practices.

Crescimento inicial do cafeeiro irrigado com água salina e salinização do solo

The coffee crop is expanding to new areas with not enough studies about its response to saline irrigation water. The initial growth of coffee plant was evaluated, in greenhouse at the Engineering Department of the Federal University of Lavras (UFLA), under different levels of irrigation water salinity. The completely randomized design was used with 6 treatments (S0 = 0.0 dS m -1, S1 = 0.6 dS m -1, S2 = 1.2 dS m -1, S3 = 1.8 dS m -1, S4 = 2.4 dS m -1 and S5 = 3.0 dS m -1) and 4 replications. The irrigation was accomplished according to soil water retention curve and resistance block reading, restoring the soil water content to its field capacity. It was verified that water salinity affected the plants characteristics significantly. The water salinity above 1.2 dS m -1 caused damage to plant development resulting, in some cases, in death of plants. The leaf area of plant was the variable most affected by salinity of irrigation water. By the end of the experiment, the soil was classified as saline-sodic.

Estudo fitoquímico preliminar e bioensaio toxicológico frente a larvas de Artemia salina Leach. de extrato obtido de frutos de Solanum lycocarpum A. St.-Hill (Solanaceae)

The phytochemical profile of ethanolic extract of Solanum lycocarpum fruits was analyzed and preliminary toxicity tests were performed against brine shrimp larvae. The extract was subjected to preliminary phytochemical analysis to identify the main classes of secondary metabolites and tested against the larvae of A. salina to obtain the median lethal concentrations (LC50%). The phytochemical tests showed the presence of phenols, tannins, saponins, alkaloids and free steroids. The extract was fractionated with various solvents for toxicity testing against the larvae and the hydroalcoholic fraction showed considerable cytotoxicity (CL50% = 285.546 g/mL).

Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro

To better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), ≥ 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39°C, whereas in the HS group, embryos were subjected to 41°C for 12 h, and then returned to 39°C. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS.

A importância do teste de tolerância à glicose oral no diagnóstico da intolerância à glicose e diabetes mellitus do tipo 2 em mulheres com síndrome dos ovários policísticos

PURPOSE: To evaluate the importance of the oral glucose tolerance test for the diagnosis of glucose intolerance (GI) and type 2 diabetes mellitus (DM-2) in women with PCOS. METHODS: A retrospective study was conducted on 247 patients with PCOS selected at random. The diagnosis of GI was obtained from the two-hour oral glucose tolerance test with 75 g of glucose according to the criteria of the World Health Organization (WHO) (GI: 120 minutes for plasma glucose =140 mg/dL and <200 mg/dL), and the diagnosis of DM-2 was obtained by both the oral glucose tolerance test (DM: 120 minutes for plasma glucose =200 mg/dL) and fasting glucose using the criteria of the American Diabetes Association (impaired fasting glucose: fasting plasma glucose =100 and <126 mg/dL; DM: fasting glucose =126 mg/dL). A logistic regression model for repeated measures was applied to compare the oral glucose tolerance test with fasting plasma glucose. ANOVA followed by the Tukey test was used for the analysis of the clinical and biochemical characteristics of patients with and without GI and/or DM-2. A p<0.05 was considered statistically significant. RESULTS: PCOS patients had a mean age of 24.8±6.3, and body mass index (BMI) of 18.3 to 54.9 kg/m2 (32.5±7.6). The percentage of obese patients was 64%, the percentage of overweight patients was 18.6% and 17.4% had healthy weight. The oral glucose tolerance test identified 14 cases of DM-2 (5.7%), while fasting glucose detected only three cases (1.2%), and the frequency of these disorders was higher with increasing age and BMI. CONCLUSIONS: The results of this study demonstrate the superiority of the oral glucose tolerance test in relation to fasting glucose in diagnosing DM-2 in young women with PCOS and should be performed in these patients.

Tolerância de Espécies de Mucuna a Herbicidas Utilizados na Cultura da Cana-de-Açúcar

The emergence of Mucuna species in sugarcane plantations, even after herbicide spraying for weed management, allowed us to elaborate the hypothesis that these plants are tolerant to the herbicides commonly used in the culture. Thus, to prove this hypothesis, this work was carried out to study the tolerance of Mucuna aterrima, Mucuna cinerea and Mucuna deeringiana to application of herbicides with different mechanisms of action in pre- and postemergence. The experiment was arranged in a randomized block design with 9 treatments and five replications in a factorial 3 × 3, plus controls. At pre-emergence, the first factor was constituted by the herbicides sulfentrazone (800 g ha-1), imazapic (245 g ha-1), and amicarbazone (1,400 g ha-1), and the second factor by the Mucuna species, besides the additional control for each species studied. At post- emergence, the herbicides tested were clomazone (1,100 g ha-1), ametryn+trifloxysulfuronsodium (1,463 + 37 g ha-1), and 2,4-D (1,209 g ha-1). In the chemical management at pre-emergence, the species were sensitive to amicarbazone, followed by sulfentrazone, and tolerant to imazapic. At post-emergence, all species were sensitive to ametryn + trifloxysulfuron-sodium and 2,4-D, but tolerant to clomazone.

Tolerância à deficiência hídrica de genótipos de arroz de terras altas

Water deficit is one of the environmental factors that cause a great reduction in the upland rice grain yield. The objective of this study was to evaluate genotypes of upland rice with broad genetic diversity for tolerance to this stress and to identify secondary traits for evaluation of this tolerance. Sixty-four genotypes were evaluated during two years at the Experiment Station of EMATER, in Porangatu, GO, under well-watered and water deficit conditions. The genotypes showed differences in grain yield and were influenced differently by the water treatments, both under a mild water deficit in the first year and under a severe one in the second year. The genotype grain yield under water deficiency was significantly and positively correlated with tiller fertility, number of panicles m-2, 100-grain weight, and plant height, and negatively with spikelet sterility and the number of days for the occurrence of 50% panicle emergence. It was also observed that the most productive genotypes under water deficit conditions showed lower leaf temperature, indicating improved water status. Nine genotypes were productive in both water conditions and three were productive only when well-watered.

Tolerância à deficiência hídrica em cultivares de cana-de-açúcar avaliada por meio de variáveis morfofisiológicas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Tolerância de Digitaria ciliaris, Digitaria horizontalis e Digitaria nuda à herbicidas usados em cana-de-açúcar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Silício na tolerância ao alumínio por plantas de arroz

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da tolerância de leveduras a um coquetel de inibidores que simula um hidrolisado de bagaço de cana quando adicionado a um meio sintético

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uma contribuição para o estudo do efeito causado pela névoa salina em isoladores cerâmicos de chaves fusíveis

Pós-graduação em Ciência e Tecnologia de Materiais - FC