Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture

Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development. At the 10th Keele meeting, we reported that long-term exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process. We now report that long-term exposure (20–25 weeks) to Al chloride or Al chlorohydrate at 10−4 M or 10−5Mconcentrations can also increase themigration of oestrogen unresponsiveMDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence technology. In parallel, Al exposure was found to give rise to increased secretion of active matrixmetalloproteinaseMMP9 as measured by zymography, and increased intracellular levels of activated MMP14 as measured by western immunoblotting. These results demonstrate that Al can increase migration of human breast cancer cells irrespective of their oestrogen responsiveness, and implicate alterations to MMPs as a potential mechanism worthy of further study.

Regulation of Multidrug Resistance-Associated Protein 2 by Calcium Signaling in Mouse Liver

Mu hiding resistance associated protein 2 (Mrp2) is a canalicular transporter responsible for organic anion secretion into bile. Mrp2 activity is regulated by insertion into the plasma membrane; however, the factors that control this are not understood. Calcium (Ca(2+)) signaling regulates exocytosis of vesicles in most cell types, and the type II inositol 1,4,5-triphosphate receptor (InsP(3)R2) regulates Ca(2+) release in the canalicular region of hepatocytes. However, the role of InsP(3)R2 and of Ca(2+) signals in canalicular insertion and function of Mrp2 is not known. The aim of this study was to determine the role of InsP(3)R2-mediated Ca(2+) signals in targeting Mrp2 to the canalicular membrane. Livers, isolated hepatocytes, and hepatocytes in collagen sandwich culture from wild-type (WT) and InsP(3)R2 knockout (KO) mice were used for western blots, confocal immunofluorescence, and time-lapse imaging of Ca(2+) signals and of secretion of a fluorescent organic anion. Plasma membrane insertion of green fluorescent protein (GFP)-Mrp2 expressed in HepG2 cells was monitored by total internal reflection microscopy. InsP(3)R2 was concentrated in the canalicular region of WT mice but absent in InsP(3)R2 KO livers, whereas expression and localization of InsP(3)R1 was preserved, and InsP(3)R3 was absent from both WT and KO livers. Ca(2+) signals induced by either adenosine triphosphate (ATP) or vasopressin were impaired in hepatocytes lacking InsP(3)R2. Canalicular secretion of the organic anion 5-chloromethylfluorescein diacetate (CMFDA) was reduced in KO hepatocytes, as well as in WT hepatocytes treated with 1,2-bis(o-aminophenoxy)ethane-N,N,N`,N`-tetra-acetic acid (BAPTA). Moreover, the choleretic effect of tauroursodeoxycholic acid (TUDCA) was impaired in InsP(3)R2 KO mice. Finally, ATP increased GFP-Mrp2 fluorescence in the plasma membrane of HepG2 cells, and this also was reduced by BAPTA. Conclusion: InsP(3)R2-mediated Ca(2+) signals enhance organic anion secretion into bile by targeting Mrp2 to the canalicular membrane. (HEPATOLOGY 2010;52:327-337)

The geodiamolide H, derived from Brazilian sponge Geodia corticostylifera, regulates actin cytoskeleton, migration and invasion of breast cancer cells cultured in three-dimensional environment

We are investigating effects of the depsipeptide geodiamolide H, isolated from the Brazilian sponge Geodia corticostylifera, on cancer cell lines grown in 3D environment. As shown previously geodiamolide H disrupts actin cytoskeleton in both sea urchin eggs and breast cancer cell monolayers. We used a normal mammary epithelial cell line MCF 10A that in 3D assay results formation of polarized spheroids. We also used cell lines derived from breast tumors with different degrees of differentiation: MCF7 positive for estrogen receptor and the Hs578T, negative for hormone receptors. Cells were placed on top of Matrigel. Spheroids obtained from these cultures were treated with geodiamolide H. Control and treated samples were analyzed by light and confocal microscopy. Geodiamolide H dramatically affected the poorly differentiated and aggressive Hs578T cell line. The peptide reverted HsS78T malignant phenotype to polarized spheroid-like structures. MCF7 cells treated by geodiamolide H exhibited polarization compared to controls. Geodiamolide H induced striking phenotypic modifications in Hs578T cell line and disruption of actin cytoskeleton. We investigated effects of geodiamolide H on migration and invasion of Hs578T cells. Time-lapse microscopy showed that the peptide inhibited migration of these cells in a dose-dependent manner. Furthermore invasion assays revealed that geodiamolide H induced a 30% decrease on invasive behavior of Hs578T cells. Our results suggest that geodiamolide H inhibits migration and invasion of Hs578T cells probably through modifications in actin cytoskeleton. The fact that normal cell lines were not affected by treatment with geodiamolide H stimulates new studies towards therapeutic use for this peptide.

Boerdery

"A time-lapse document of a farm house in the Netherlands mapping the changing seasons, the light and shadows." http://www.innersense.com.au/mif/debruyn_films.html

Byron Bay hysteria

"This time lapse video work of De Bruyn's evidences the bold rythmic structures found in his nahd-painted 16 mm film work. A prolific fimmaler, de Bruyn is a master of his craft and shows in this work that that mastery knows no bounds in this format."  Fractured Light exhibition catalogue

Byron Bay

Another time-lapse video piece by de Bruyn, Byron Bay #04/09 evokes nostalgia in its subject matter of the coastline of northern New South Wales.

Tuning fork: Shopfront

The audience of this performance got to view actions and installations building and evolving over time, and a projected time-lapse video.
In this iteration of Tuning Fork, James Cunningham & Joncli Keane continue the development of their two pronged approach to finding the watering hole of stillness and action, collabora tion and orientation.Originating from a showing in the Bell Tower II series, in the Judy's new Shopfrontspaoe, Tuning Fork will Fuks accentuate the durational aspect of movement through time-lapse video, heighten the trans formative potential of objects using three-metre long carbon rods as instruments of sense, sound and support and alter the space through the numerous tape measures which take on a life of their own. James Cunningham and Jondi Keane inhabit the Shopfront from Friday 7 November, eJqJioring, measuring, filming and projecting, in order to fine tune their interactions for the performance season commencing 19 November.

Opening

Opening, a collaboration between Paul Carter, Dirk de Bruyn and Soo Yeun You, combines the Nearamnew artwork etched in the bed of Fed Square’s river of cobblestones with the oral histories and creative research expressed in Ground Truthing.

Opening brings together single frame and time lapse techniques, real-time footage, environmental recordings and choreography in a 37-minute creative manifestation.

This is a unique and special project which brings to life the history, land, design and community of Fed Square. It will transport you through the curving path that spirals out to Lake Tyrrell in Northern Victoria and whirls back to Fed Square.

Telescope

Telescope is a feature length time lapse of reflections, changes in sunlight of my backyard, assembled over 20 years. Telescope starts in Super 8 and ends with digital video, shot mostly while the family were themselves at work, somewhere else. It is an emptied landscape. When people think of Australia they imagine open space and bush. But really most Australians inhabit or were born in suburban spaces, often with backyards with fences, big enough for fruit trees, lawns and clotheslines. I consider this a place of absence that speaks to many things that our culture avoids.The backyard as emblem of a White Australia that hit its highpoint in the 1950s, for example. Australia is a migrant culture settled by waves of newcomers escaping, running away from somewhere else, leaving to forget. Another story concerns the continued invisibility of the indigenous people. When the British first planted the Union Jack on Australian soil and said "there is nothing here" they set up a tradition of denial as our founding principle. This still plagues us. What is festering in Australian backyards are these denials and erasures that I try to bring out in the soundtrack, that plays like the radio that meanders through a lazy Sunday afternoon. Such sounds try to tell stories of absence, of occupation, and of a nostalgia for an Australia that no longer exists, but still palpably reverberates around the suburban backyard.

Experiments

Projected on two screens, with two separate soundtracks, the always exceptional, and occasionally brilliant, photographic images are enhanced by Dirk de Bruyn's rigorous control over a wide variety of experimental techniques.

Without overindulging in any of them, de Bruyn uses animation, optical illusions, time lapse, solarization, hand tinting, flash frames, refilming and flicker effects, accompanied by a dense atmosphere of word puns, dialogue, primal screams, music and even recycled and letraseted soundtracks.

Expanding horizon (Reykjavík)

Expanding horizons (Reykjavík) (2012-2013) is part of an ongoing series of interactive net-art/installation works that explores the space of the horizon line. Produced with time-lapse photography, animated movements, and playing with perception, this work explores how the vastness of a horizon line can be captured, compressed and re-presented for viewers to align with as an artwork

Telescope

Assembled from more than 25 years of footage, starting in Super 8 and ending with digital video, Melbourne-based experimental filmmaker Dirk de Bruyn's lens captures the comings and goings of Australian suburban life. A nostalgia-filled soundtrack accompanies footage of empty backyards, over-crowded lounge rooms and sun-drenched porches. Interspersed with line-animation, de Bruyn recalls an Australia that no longer exists in this time-lapse feature length film.

Found Found Found

Through time-lapse and pixilated animation, recorded on the run through Serbia, Europe, international air travel through Australasia, and including recordings at the 2013 Christmas Markets in Dusseldorf, this short roaming personal narrative contemplates our current pre-occupation with mobile technologies and the concomitant reshaping of everyday life and public space. It features one extreme response to technological and political change: Alex Jones’ Infowars radio program. The film suggests surveillance, metamorphosed from avant-garde and minimalist cinema, as the ‘new norm’, and witnesses the new stasis that hypermobility institutes globally and the florid thinking it elicits.

Activities between activities of focus-Relevant when assessing DNA transfer probabilities

Consideration of the indirect transfer of 'touch DNA' is increasingly becoming part of criminal investigations. Focus is often concentrated on the actions relating to the pick-up of the relevant DNA and key actions associated with transfer to the exhibit from which the sample in question was collected. There is often a time lapse between such actions. As any contact can influence the gain and/or loss of DNA, it is relevant to have an awareness of what hands touch during everyday activities in order to assist consideration of what may be occurring during potential time lapses within contemplated scenarios. To gain an appreciation of the manner and frequency of hands contacting various surfaces during everyday activities, we analysed several videos of individuals performing a variety of general activities. The findings indicate that several items are touched over a relatively short period of time. Appreciation and consideration of general activities that may have occurred between key focus activities are necessary to assess any impact these may have on what is deposited at the final collection site. The information this provides is imperative when weighting alternative transfer scenario propositions.

Structural, functional, and bioinformatics studies reveal a new snake venom homologue phospholipase A(2) class

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)