The role of lakes in carbon cycling in boreal catchments

Lakes are an important component of ecosystem carbon cycle through both organic carbon sequestration and carbon dioxide and methane emissions, although they cover only a small fraction of the Earth's surface area. Lake sediments are considered to be one of rather perma-nent sinks of carbon in boreal regions and furthermore, freshwater ecosystems process large amounts of carbon originating from terrestrial sources. These carbon fluxes are highly uncer-tain especially in the changing climate. -- The present study provides a large-scale view on carbon sources and fluxes in boreal lakes situated in different landscapes. We present carbon concentrations in water, pools in lake se-diments, and carbon gas (CO2 and CH4) fluxes from lakes. The study is based on spatially extensive and randomly selected Nordic Lake Survey (NLS) database with 874 lakes. The large database allows the identification of the various factors (lake size, climate, and catchment land use) determining lake water carbon concentrations, pools and gas fluxes in different types of lakes along a latitudinal gradient from 60oN to 69oN. Lakes in different landscapes vary in their carbon quantity and quality. Carbon (C) content (total organic and inorganic carbon) in lakes is highest in agriculture and peatland dominated areas. In peatland rich areas organic carbon dominated in lakes but in agricultural areas both organic and inorganic C concentrations were high. Total inorganic carbon in the lake water was strongly dependent on the bedrock and soil quality in the catchment, especially in areas where human influence in the catchment is low. In inhabited areas both agriculture and habitation in the catchment increase lake TIC concentrations, since in the disturbed soils both weathering and leaching are presumably more efficient than in pristine areas. TOC concentrations in lakes were related to either catchment sources, mainly peatlands, or to retention in the upper watercourses. Retention as a regulator of the TOC concentrations dominated in southern Finland, whereas the peatland sources were important in northern Finland. The homogeneous land use in the north and the restricted catchment sources of TOC contribute to the close relationship between peatlands and the TOC concentrations in the northern lakes. In southern Finland the more favorable climate for degradation and the multiple sources of TOC in the mixed land use highlight the importance of retention. Carbon processing was intensive in the small lakes. Both CO2 emission and the Holocene C pool in sediments per square meter of the lake area were highest in the smallest lakes. How-ever, because the total area of the small lakes on the areal level is limited, the large lakes are important units in C processing in the landscape. Both CO2 and CH4 concentrations and emissions were high in eutrophic lakes. High availability of nutrients and the fresh organic matter enhance degradation in these lakes. Eutrophic lakes are often small and shallow, enabling high contact between the water column and the sediment. At the landscape level, the lakes in agricultural areas are often eutrophic due to fertile soils and fertilization of the catchments, and therefore they also showed the highest CO2 and CH4 concentrations. Export from the catchments and in-lake degradation were suggested to be equally important sources of CO2 and CH4 in fall when the lake water column was intensively mixed and the transport of sub-stances from the catchment was high due to the rainy season. In the stagnant periods, especially in the winter, in-lake degradation as a gas source was highlighted due to minimal mixing and limited transport of C from the catchment. The strong relationship between the annual CO2 level of lakes and the annual precipitation suggests that climate change can have a major impact on C cycling in the catchments. Increase in precipitation enhances DOC export from the catchments and leads to increasing greenhouse gas emissions from lakes. The total annual CO2 emission from Finnish lakes was estimated to be 1400 Gg C a-1. The total lake sediment C pool in Finland was estimated to be 0.62 Pg, giving an annual sink in Finnish lakes of 65 Gg C a-1.

Mykorritsa fosforin kierrättäjänä

Suomen maatalousmaihin kertynyttä fosforia hyödynnetään tehottomasti, ja samalla muokkauskerroksen suuri fosforimäärä on alttiina huuhtoutumiselle. Arbuskelimykorritsaa (AM) hyödyntämällä on mahdollista tehostaa viljelykasvin fosforinottoa ja kasvua, ja siten vähentää fosforin huuhtoutumista. Tämän tutkielman tavoitteena oli selvittää mykorritsan vaikutus kasvin kasvuun ja fosforinottoon karjanlantalannoituksella mineraalilannoitukseen verrattuna sekä näiden lannoitusten pitkäaikaisvaikutusta AM-sieniyhteisöihin. Jotta lannoituskäytäntöjen vaikutus mykorritsaan voitiin suhteuttaa muihin maan laatutekijöihin, näiden käytäntöjen vaikutus myös satomääriin sekä muihin maan laatumittareihin arvioitiin. Pitkäaikainen kenttäkoe perustettiin kolmelle paikkakunnalle Pohjois-Ruotsissa vuosina 1965–66. Kuusivuotinen viljelykierto koostui joko viisivuotisesta nurmesta ja ohrasta tai ohramonokulttuurista. Lannoituskäsittelyt 32-vuoden ajan olivat suositusten mukainen (NPK) ja edelliseen nähden kaksinkertainen (2NPK) mineraalilannoitus sekä karjanlantalannoitus (KL), jonka ravinnemäärä vastasi NPK -käsittelyä. Kolmen lannoituskäsittelyn vaikutusta mykorritsan tehokkuuteen kasvin kasvun ja fosforiravitsemuksen näkökulmasta tutkittiin astiakokeissa. Mykorritsasieniyhteisöjen toiminnallisten erojen selvittämiseksi tehtiin takaisin- ja ristiinsiirrostuskoe. (5 v-%) steriloitua maanäytettä NPK- ja KL -käsittelyistä siirrostettiin käsittelemättömiin maanäytteisiin, jotka olivat samoista lannoituskäsittelyistä. Mykorritsan positiivinen vaikutus kasvin kasvuun ja fosforiravitsemukseen oli suurin kun käytettiin karjanlantaa. NPK ja 2NPK -käsittelyiden välillä ei havaittu eroja. Takaisin- ja ristiinsiirrostuskokeessa ei ollut tilastollisesti merkitseviä eroja. Nurmi- ja ohrasadot olivat suurimmat kun mineraalilannoitetta annettiin suosituksiin nähden kaksinkertainen määrä. Satomäärät olivat yhtä suuret tai suuremmat kun käytettiin karjanlantaa NPK –lannoituksen sijaan. Karjanlantakäsittely lisäsi maaperän kokonaishiili- ja kokonaistyppipitoisuutta verrattuna NPK -käsittelyyn, joka sisälsi saman määrän ravinteita. Samalla huuhtoutumiselle altis liukoisen fosforin pitoisuus säilyi alhaisella tasolla. Karjanlanta edisti mykorritsan toimintaedellytyksiä, ja siksi mykorritsasta saatua hyötyä fosforinotossa ja kasvuvaikutuksena mineraalilannoitteisiin verrattuna, mutta se ei vaikuttanut mykorritsasieniyhteisön toiminnallisiin ominaisuuksiin. Karjanlantalannoitus paransi mitattuja maan ominaisuuksia kokonaisuudessaan, eikä se vähentänyt satoja.

The potential of microbial ecological indicators to guide ecosophisticated management of hydrocarbon-contaminated soils

Soil is an unrenewable natural resource under increasing anthropogenic pressure. One of the main threats to soils, compromising their ability to provide us with the goods and ecosystem services we expect, is pollution. Oil hydrocarbons are the most common soil contaminants, and they disturb not just the biota but also the physicochemical properties of soils. Indigenous soil micro-organisms respond rapidly to changes in the soil ecosystem, and are chronically in direct contact with the hydrophobic pollutants on the soil surfaces. Soil microbial variables could thus serve as an intrinsically relevant indicator of soil quality, to be used in the ecological risk assessment of contaminated and remediated soils. Two contrasting studies were designed to investigate soil microbial ecological responses to hydrocarbons, together with parallel changes in soil physicochemical and ecotoxicological properties. The aim was to identify quantitative or qualitative microbiological variables that would be practicable and broadly applicable for the assessment of the quality and restoration of oil-polluted soil. Soil bacteria commonly react on hydrocarbons as a beneficial substrate, which lead to a positive response in the classical microbiological soil quality indicators; negative impacts were accurately reflected only after severe contamination. Hydrocarbon contaminants become less bioavailable due to weathering processes, and their potentially toxic effects decrease faster than the total concentration. Indigenous hydrocarbon degrader bacteria, naturally present in any terrestrial environment, use specific mechanisms to improve access to the hydrocarbon molecules adsorbed on soil surfaces. Thus when contaminants are unavailable even to the specialised degraders, they should pose no hazard to other biota either. Change in the ratio of hydrocarbon degrader numbers to total microbes was detected to predictably indicate pollutant effects and bioavailability. Also bacterial diversity, a qualitative community characteristic, decreased as a response to hydrocarbons. Stabilisation of community evenness, and community structure that reflected clean reference soil, indicated community recovery. If long-term temporal monitoring is difficult and appropriate clean reference soil unavailable, such comparison could possibly be based on DNA-based community analysis, reflecting past+present, and RNA-based community analysis, showing exclusively present conditions. Microbial ecological indicators cannot replace chemical oil analyses, but they are theoretically relevant and operationally practicable additional tools for ecological risk assessment. As such, they can guide ecologically informed and sustainable ecosophisticated management of oil-contaminated lands.

Modelling the Effect of Climate Change on Environmental Pollution Losses from Dairy Systems in the UK

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Caracterização química de um solo contaminado por resíduos sólidos urbanos na estrada Arroio Pavuna em Jacarepaguá no município Rio de Janeiro

Neste trabalho, escolheu-se a estrada canal Arroio Pavuna, em Jacarepaguá, para avaliar quimicamente a qualidade do solo que recebe diariamente resíduos sólidos urbanos. A região deste estudo é também conhecida como estrada do Urubu, sendo este animal um dos principais vetores presente no local. Para uma caracterização química, foram determinados pH, carbono orgânico, matéria orgânica, concentrações dos metais alumínio, cádmio, chumbo, cobre, cromo, ferro, níquel e zinco, utilizando a técnica de Absorção Atômica por Chama, além da identificação dos principais compostos orgânicos através da técnica de Cromatografia Gasosa acoplada a Espectrometria de Massas. Também foi realizado o teste de comportamento com oligoquetas da espécie Eisenia andrei para avaliar a função de habitat do solo analisado. Os resultados obtidos apontaram que a região apresenta um solo ácido na faixa de 5,51 a 6,70, bem como as concentrações de Cobre, Cromo, Níquel e Zinco acima dos valores orientadores de referência de qualidade dos solos, um teor médio de matéria orgânica na faixa de 21,71 g.kg-1 a 29,73 g.kg-1, além de presença de compostos orgânicos com estruturas muito complexas. O solo apresentou função de habitat limitada. E como sugestão para a remediação deste solo, a fitorremediação apresenta bons rendimentos na literatura para remoção parcial ou total desses metais

Avaliação da influência do manejo agrícola no uso e cobertura das terras através de ferramentas de geoprocessamento e indicadores de qualidade dos solos na bacia hidrográfica do Córrego da Caçada - Paraty/RJ

A avaliação da qualidade dos solos e a redução da Mata Atlântica tem sido alvo de diversas pesquisas no Brasil e no mundo, principalmente quando estes estão atrelados ao recorte espacial de Unidades de Conservação. No entanto, tem sido difícil para os gestores dessas unidades a avaliação dos impactos ambientais gerados ao longo dos anos nas bacias hidrográficas, principalmente pela falta de investimentos. Esta dissertação teve por objetivo geral avaliar o atual estágio de degradação da bacia hidrográfica do Córrego da Caçada pertencente à Área de Proteção Ambiental Federal de Cairuçu, no município de Paraty RJ, analisando quantitativamente e qualitativamente a redução dos fragmentos de Mata Atlântica e estabelecendo relações com a degradação física e química dos solos dentro e fora dos fragmentos florestais. A metodologia utilizada para a redução ou avanço dos fragmentos de Mata Atlântica baseou-se no uso de fotografias aéreas do ano de 1956 e imagens de satélite de 2012, onde possibilitou a avaliação espaço-temporal do uso e cobertura das terras, através da produção de um mapa temático final. Além disso, foram elaborados mapas temáticos de reconhecimento da área de estudo, como o de hipsometria, de declividade, de orientação e forma das encostas, além da geração de perfis topográficos. Para a avaliação da qualidade física, química e biológica dos solos foram determinadas as curvas de distribuição granulométrica, a densidade relativa dos grãos sólidos e a densidade aparente, porosidade total, os limites de liquidez e plasticidade, a estabilidade dos agregados em água, análises morfológicas, a saturação de bases, a capacidade de troca catiônica (CTC), a saturação por alumínio, fósforo, pH e o carbono orgânico. Para tal, foi realizada a abertura de três perfis, sendo um em área de fragmento florestal e dois em áreas de pasto. O resultado das análises permitiu, segundo a Sociedade Brasileira de Ciência do solo, a classificação de dois tipos de solos na bacia, sendo: Cambissolo Háplico Tb Distrófico Típico em área fragmento florestal e em área de pasto, e um Latossolo Amarelo Tb Distrófico Típico em área de pasto. Os resultados de laboratório mostraram que os solos avaliados têm baixa fertilidade e valores variados nos resultados de física do solo. No entanto, além do histórico de uso do solo caracterizado pelas práticas rudimentares do manejo empregado pelos Caiçaras, o clima predominante na região possibilita um regime pluviométrico anual que passa dos 2.000mm de chuva/ano, caracterizando solos muito lixiviados e pobres quimicamente. Portanto, conclui-se que a relação das propriedades físicas e químicas avaliadas junto ao manejo inadequado ao longo dos anos tem apresentado um cenário de grandes dificuldades para a recuperação florestal na bacia hidrográfica do Córrego da Caçada, o que mostra a importância da avaliação dos impactos ambientais não só pelo recorte de bacias hidrográficas, como contextualizar seu posicionamento dentro de Unidades de Conservação, com legislações e objetivos específicos.

云雾山不同草地群落土壤活性有机碳分布特征

为探讨黄土高原草地植被演替过程活性有机碳的变化规律,揭示草地退耕后的分布特征,在云雾山草原区采集不同草地群落土壤,采用高锰酸钾氧化法对0~100 cm土层活性有机碳进行分析。结果表明:不同草地群落土壤活性有机碳含量均高于坡耕地,活性有机碳含量大小顺序为:长芒草(Stipa bungeanaTrin.)群落>大针茅(Sti-pa grandisP.Smirn.)群落>铁杆蒿(Artemisia gmeliniiWeb.ex Stechm.)群落>百里香(Thymus mongolicusRonn.)群落>退耕草地>坡耕地,且群落间差异达极显著水平(P<0.01);在恢复初期(坡耕地-退耕草地-百里香)活性有机碳含量增加较多,恢复到长芒草群落活性有机碳含量达到最大值,趋于稳定;活性有机碳的密度与含量表现出相同的变化规律,并随着土层的加深而呈现减小趋势。相关性分析表明,活性有机碳含量与土壤总有机碳含量呈极显著线性正相关关系(r=0.9742),土壤活性有机碳比总有机碳更能反映草地植被恢复初期土壤有机碳库的变化。

基于土壤质量的保护性农业技术及其政策取向

与保护性耕作不同,保护性农业是世界农业发展史上一次全新的思想革命。本文总结分析了保护性农业的概念及其特点,结合国内外土壤质量研究的发展,阐述了保护性农业与土壤质量研究新进展,并提出我国保护性农业的发展方向。

黄土高原子午岭地区植被恢复过程中的土壤质量评价

选择黄土高原子午岭地区植被自然恢复1~140 a不同年限的阳坡坡地为研究对象,通过相关性分析筛选土壤表层(0~20 cm)16项表征土壤物理、化学、生物学性质的指标,运用主成分分析计算土壤质量综合指数,评价子午岭地区植被恢复过程对土壤质量的影响。结果表明:土壤总孔隙度、平均重量直径(MWD)、有机质质量分数、速效磷质量分数、蔗糖酶活性、碱性磷酸酶活性、真菌数量、微生物总量构成土壤质量评价指标体系;土壤质量综合指数随植被恢复年限的增加而增加;植被自然恢复1~140 a间,土壤质量综合指数变化范围为0.155 9~0.850 9,较裸露休闲地增加4.2~27.5倍;根据土壤质量综合指数变化规律,可将140 a植被恢复过程中的土壤质量演变过程分为3个阶段,即植被恢复初期(1~20 a)的土壤质量综合指数呈快速增长,植被恢复中期(20~40 a)的土壤质量综合指数呈波动性增长,植被恢复后期(40~140 a)的土壤质量综合指数呈稳定增长。植被演替过程中不同植被生活型土壤质量综合指数表现为乔木林地>灌木林地>草地。

黄土高原水蚀风蚀交错带不同土地利用方式坡面土壤水分特性研究

为分析不同土地利用方式下坡面土壤水分特性的差异和规律,对黄土高原水蚀风蚀交错带典型坡面上农地、林地和草地的土壤水分特性进行了分析。结果表明:(1)苜蓿地土壤的持水能力和供水能力最强,其次是杏树林和长芒草地,谷子地最差;(2)通过土壤水分有效性分析发现,水蚀风蚀交错带田间持水量相当于-0.2×105Pa土壤基质势时的土壤含水量,永久凋萎点则低于-2.0×106Pa土壤基质势对应的土壤含水量;(3)苜蓿地有效水含量最高,谷子地有效水含量最低,而且苜蓿地土壤有效水含量的提高主要是提高了迟效水部分;(4)对不同土地利用方式下的土壤比水容量变化曲线研究表明,土壤比水容量在田间持水量附近随土壤含水量的降低减小的很快,而当土壤水分降低到田间持水量的50%~60%以下时,土壤比水容量基本不变。

黄土高原沟壑区不同年限苹果园土壤碳、氮、磷变化特征

管理措施是影响土壤质量演变的重要因素。分析和讨论了5、101、5年苹果园耕层(0—20 cm)和0—200 cm土壤有机碳、全氮、全磷、有效磷和硝态氮含量及其影响因素。结果表明,5年、10年和15年的塬面苹果园表层土壤有机碳依次为7.5、6.7和6.7 g/kg;全氮依次为0.940、.85和0.83 g/kg;但土壤全磷和速效磷含量随着种植年限而增加,与5年苹果园相比,塬面10年苹果园土壤全磷、速效磷含量分别提高了11%、60%,并且磷素的变异性随年限而增加。坡地10年、15年和20年苹果园土壤有机碳依次为6.36、.2和6.5 g/kg,全氮依次为0.76、0.76和0.81 g/kg;与10年苹果园相比,15年苹果园土壤全磷、速效磷含量分别提高了20%、28%。土壤剖面0—80 cm内不同土地利用方式土壤碳、氮、磷含量随土层加深而降低,80 cm以下不同利用条件苹果园土壤碳、磷含量差异不大,氮素含量在100 cm土层下随苹果园种植年限增加而增加。

施肥和海洋微生物调控对桉树人工林土壤质量的影响

本文研究施化肥和海洋微生物制剂对桉树人工林土壤质量的影响。研究结果表明：施用不同的化肥对桉树人工林土壤质量影响具有明显差异；从海洋植物根际分离得到的微生物菌株制成的微生物制剂中的活性微生物菌株能够在桉树人工林土壤中定殖，对桉树生长具有一定促进作用。桉树凋落叶分解过程中是否释放化感物质是桉树人工林发展过程中人们普遍关注的问题之一，本论文也对该部分做了初步研究。 对施用长效尿素、芬兰复合肥、高峰复合肥三种不同化肥对桉树人工林土壤质量的的影响进行了初步研究，研究结果表明长效尿素在保障土壤氮素供应、促进土壤纤维素分离能力提高和增强土壤对磷元素吸收方面具有重要作用；芬兰复合肥在增强土壤呼吸作用和促进土壤酶活性提高方面优于长效尿素和高峰复合肥。 以两株海洋来源的枯草芽孢杆菌(Bacillus subtilis 3512, Bacillus subtilis 3728)和一株海洋木霉(Trichoderma TF4)为研究材料，在实验室条件下对其生防机理进行了研究，研究表明:两株枯草芽孢杆菌通过产生脂肽和蛋白酶对植物病原菌产生抑制作用；海洋木霉TF4则能够产生HCN，IAA类植物生长激素，同时还具有一定的解磷能力，具有很好的应用前途，采用传统分类学方法和分子系统学方法鉴定为棘孢木霉(T.asperellum)。这三株海洋菌株制成微生物菌剂，在原位条件和盆栽条件下考察了其对桉树生物量和土壤质量指标的影响，研究结果表明将三株海洋微生物混合后添加少量三叶草作辅剂，能有效改善桉树人工林土壤质量，并促进桉树树高和胸径的增加，具备进一步研究和开发成产品的价值。 用高效液相色谱(HPLC)对桉树凋落叶中毒性物质进行分离、纯化，以小麦、绿豆、大速生菜为指示植物，对分离到的物质进行毒性跟踪，分离到一个毒性较强的组分，经1H氢谱和NMR鉴定为3–β甲酰基–乌索酸。

氮肥对黄瓜根际土壤线虫群落组成、结构及其多样性的影响

土壤线虫是土壤动物的主要功能类群之一，在土壤养分分解和循环中起到重要的作用。本研究通过施用两种形态氮肥，硝态氮(NO-3–N)和铵态氮(NH+4–N)，对黄瓜整个生长期内根际土壤线虫的群落组成、结构及其多样性等的影响进行了比较研究。为增进土壤健康，提高土壤质量以及合理施用氮肥提供科学的理论依据。研究结果如下： 1. 氮肥处理后，不同浓度NO-3–N处理提高了根际土壤线虫数量，而NH+4–N处理（202.5 kg N/ hm2）抑制了线虫数量的增加。线虫群落结构相对较稳定，营养类群变化不大。且少量优势科/属会对土壤线虫群落特征起着至关重要的作用。 2. 在整个生长季节内，非寄生线虫的群体动态变化与寄生线虫的群体的动态变化具有相反的变化趋势。其中，NO-3–N处理和NH+4–N处理后植物寄生线虫出现频率的变化趋势相近，都是由高到低；非植物寄生线虫出现频率的变化趋势则都是由低到高。这说明非植物寄生线虫数量增长和空间占位对植物寄生线虫群体有一定的抑制作用。另外，也反映了适量氮肥在一定程度上能够减轻植物寄生线虫对黄瓜的危害。 3. 由多样性指数变化可知，NO-3–N和NH+4–N在低肥（67.5 kg N/hm2）和高肥（202.5 kg N/hm2）处理较中肥（135.0 kg N/hm2）处理，更不利于提高土壤线虫多样性地提高和线虫群落的稳定性。中肥不同形态氮肥处理与对照相比，H´指数在初花期和结果期显著增加了土壤线虫的H´指数，说明施入两种形态的氮肥能够提高线虫生物多样性程度。NH+4–N处理在初花期和结果期显著降低了土壤线虫种类的丰富性。土壤线虫生物多样性变化中，H´和SR指数在一定程度上能够反映施用无机氮肥对土壤线虫的多样性的影响，而J指数和λ指数效果不明显。NO-3–N和NH+4–N处理相比较，NO-3–N处理对黄瓜土壤线虫的多样性指数影响更大，促进了土壤线虫群体的多样化和种类的丰富度，更有利于提高土壤线虫的多样性，增加其稳定性。这些结果表明适量无机氮肥特别是NO-3–N的施用对黄瓜土壤线虫的生物多样性有一定的维护和提高作用。 4. 线虫数量与土壤质量指标的相关分析表明：线虫数量与有关土壤理化生指标，如土壤NO-3–N、NH+4–N、有机质含量等的正相关程度高，与总酚含量等显著负相关；与根际土壤微生物，细菌、真菌、放线菌数量等呈显著正相关。另外，线虫数量与土壤含水量未表现出显著相关关系。 Nematodes play a major role in decomposition and nutrient cycling in soil. Nematode community analyses are useful in assessing soil ecosystem status and function. The effects of two forms of mineral nitrogenous fertilizers (NO-3–N and NH+4–N) on nematode community composition, structure and diversity in rhizosphere of cucumber were investigated during different growing seasons of cucumber. Systematically research of effects of nitrogenous fertilizers could help to obtain better undstanding of a healthy soil and using nitrogenous fertilizers in reason. The main results are as follows: 1. The total numbers of nematode were more abundant in NH+4–N treatments than other teatments. However, NH+4–N teatment（202.5 kg N/hm2）dramatically inhibited it. All the tropic groups in the soil nematode communities were stable, and the dominant family or genus had an important function in the nematode community structure. 2. There was similar trend of the frequency of plant parasitic nematodes between NO-3–N and NH+4–N treatment, the similar trend of the frequency of non-plant parasitic nematodes was also found. But the frequency of plant parasitic nematodes exhibited a contrary trend to that of plant parasitic nematodes after different nitrogenous fertilizer treatments. The results showed that the increasing trend of the frequency and the niche of non-plant parasitic nematodes inhibited the plant parasitic nematodes, and indicated that right chemical fertilizers dosage could abate plant parasitic nematodes harm to cucumber. 3. The changes of the biodiversity index showed that the nitrogen treatment（135.0 kg N/hm2）promoted the stabilization of soil nematode diversity than other nitrogen treatments(67.5 kg N/hm2 and 202.5 kg N/hm2). In the treatment（135.0 kg N/hm2）,The changes in nematode diversity between the control plots and treated plots were compared by the biodiversity index (H´, J, SR, λ). Among these tested index, H´ and SR were effective in reflecting the effects of different nitrogenous fertilizers on the diversity of soil nematodes. In comparison with the NH+4–N treatment, the NO-3–N treatment promoted the stabilization of soil nematode diversity. 4. Correlation coefficients between nematode abundance and soil quality indices indicated that the total numbers of nematode were affected positively by NO-3–N, NH+4–N and the organic matter, and negatively by total phenolic acids; the total num- bers of nematode had positive correlation with bacteria, fungi and actinomycetes nu- mbers. Soil water contents had only a weak negative influence on it.

花椒叶和土壤浸提液对土壤微生物、土壤酶及土壤化学性质的影响

花椒（Zanthoxylum bungeanum Maxim.）是川西地区重要的经济植物，化感作用是花椒连作障碍的原因之一，而花椒凋落物和根系分泌物对土壤质量的影响是花椒化感作用的一个重要方面。系统研究花椒如何影响土壤有助于深入理解和解决花椒连作障碍。本文主要以大红袍（10a生）花椒叶和种植过花椒的土壤的浸提液浇灌花椒幼苗进行试验，分析叶浸提液与土壤浸提液对非花椒生长土壤中土壤微生物、土壤酶及土壤化学性质的影响。主要结果如下： 1.花椒叶浸提液和土壤浸提液减少了土壤中微生物的种类、组成和数量。本试验中未施加浸提液的土样中根际微生物明显高于非根际区，在经过花椒叶浸提液处理后，根际细菌、真菌和放线菌数量以及微生物总数都有所减少，这样将会导致土壤中的有效养分的供给减少，进而可能影响植物的生长。 2．施加花椒叶浸提液和土壤浸提液，以及花椒幼苗的栽种，对不同土样中的土壤酶各有促进和抑制作用。在浸提液处理下，水解酶之间及氧化还原酶之间各存在相互促进作用。 3.施加花椒叶浸提液和土壤浸提液均抑制了根际土中全氮和有机质含量，叶浸提液还抑制了无苗土中全磷含量，土壤浸提液还抑制了无苗土中全氮含量与根际土全磷、有机质含量。但两种浸提液均促进了根际土中有效磷和水解性氮含量、根外土中全磷含量，叶浸提液促进了根际土中全磷含量，土壤浸提液促进了根外土中有效磷含量。全氮和有机质含量的下降可能对植物生长发育不利。 4．土壤化学性质与土壤酶活性在不同土样中有不同的相关性。全氮含量在施加叶浸提液的土样中与蛋白酶活性呈正相关。水解性氮含量在施加叶浸提液的土样中与蛋白酶活性、蔗糖酶活性呈正相关。全磷含量在施加叶浸提液的土样中与多酚氧化酶活性呈正相关；在施加土壤浸提液的土样中与蛋白酶活性、蔗糖酶活性呈正相关，与多酚氧化酶活性呈负相关。有效磷含量在施加叶浸提液的土样中与多酚氧化酶活性呈正相关，与蛋白酶活性呈负相关；在施加土壤浸提液的土样中与蛋白酶活性、过氧化氢酶活性呈正相关。有机质含量在施加叶浸提液的土样中与蛋白酶活性、蔗糖酶活性呈正相关。 Zanthoxylum bungeanum is one of the most important cash crops in Eastern Tibetan Plateau. Allelopathic effects could be one of reasons for Z. bungeanum’s continuous cropping impediment. The effects of secretion of leaf and root of Z. bungeanum on soil quality is a important way of Z. bungeanum’s allelopathic effects. However, allelopathic effect of Z.bungeanum on soil microbes, enzyme activities and chemical property were seldom studied. In this study, leaf and soil extracts of Da Hongpao(DHP), the most common varieties of Z.bungeanum in this area, were used to assess allelopathic effect of Z. bungeanum on soil biology and biochemistry by pot experiments . The main results showed that: 1. The irrigation of two kinds of extracts reduced the species, component and quantity of soil microbes. In rhizosphere soil which irrigated by distilled water, the quantity of soil microbes is significantly different from exoroot soil. In rhizosphere soil which irrigated by leaf extracts, the quantity of bacterial, fungi, actionmycete and gross of microbes were decreased, it may resulted in reduce of Available nutrient in soil, and influenced the growth of plants. 2．The irrigation of two kind of extracts reduced or enhanced the enzyme activities in different soils. Interaction between hydrolytic ferments and redoxases were promoted each other. 3. The irrigation of two kinds of extracts reduced the total N and organic matter in rhizosphere soil. Leaf extracts also reduced the total P in soil without seedling. Soil extracts reduced total N in soil without seedling and total P, organic matter in rhizosphere soil. But both extracts also enhanced available P and hydrolysable N in rhizosphere soil, total P in exoroot soil. Leaf extracts enhanced total P in rhizosphere soil. Soil extracts enhanced available P in exoroot soil. The reduction of total N and organic matter may influence growth of plants. 4．Positive correlations between total N and prolease, hydrolysable N and prolease, hydrolysable N and saccharase, total P and polyphenol oxidase, available P and polyphenoloxidase, organic matter and prolease, organic matter and saccharase, were studied in soil irrigated by leaf extracts. In soil irrigated by soil extracts, there are positive correlations between total P and prolease, total P and saccharase, available P and prolease, available P and catalase, while negative correlation between total P and polyphenoloxidase, available P and prolease, available P and catalase was found.

次生植被下土壤活性有机碳组分季节动态研究

为了揭示不同类型植被下土壤有机碳及其活性组分季节动态变化及其特点，探讨不同的植被恢复模式对土壤有机碳组分的影响，分析影响土壤有机碳组分变化的因素，评估土壤有机活性有机碳组分参数在植被恢复过程中土壤质量监测的可靠性，为植被恢复及低效林改造技术提供理论依据。本研究选择岷江上游大沟流域的几种人工林（云杉林、油松林、华山松林、日本落叶松林）以及次生落叶阔叶灌丛下土壤，通过剖面机械分层取样，测定土壤总有机碳（TOC）和三种活性碳组分微生物碳（SMBC）、水溶性碳（WSOC）、易氧化碳（EOC）等来反映土壤变化特点。主要结果是： 1. 土壤有机碳含量平均在15.48~25.46 g kg-1之间在5月份时含量最低，随生长季的开始，有机碳含量逐渐增加，到9月份时含量达到最大值；由于新形成的凋落物不能被迅速分解利用补充土壤碳库，而原有碳库经历一个生长季的分解利用，因此，生长季末期即11月份的含量较小；土壤微生物碳含量平均在132.78~476.73mg kg-1之间，9月份和11月份含量都比较高；水溶性碳在生长季中逐步增大，含量在51.95~77.18 mg kg-1之间，到11月份时达到最大值；土壤易氧化碳平均含量在3.74~5.79g kg-1之间，含量最低值出现在5月份，但和其他碳组分不同的是其在7月份时含量较高。 2. 土壤有机碳及其活性碳组分大小关系为：TOC>EOC >SMBC>WSOC；比值约为300：70：5：1。 3. 土壤不同层次间比较，土壤碳指标都表现为随土壤深度增加而逐渐减小, 表层积聚作用明显。 4. 对土壤总有机碳量与活性碳组分以及活性碳之间进行了相关分析表明，土壤总有机碳含量与土壤微生物量碳、水溶性碳、易氧化碳之间的相关性均达到显著水平（P<0.05），有机碳总贮量很大程度上制约着土壤活性碳组分。土壤微生物量碳、水溶性碳、易氧化碳两两之间也都存在着显著相关关系（P<0.05），并随着不同植被类型或立地条件因子发生变化而变化。 5. 土壤有机碳及其活性组分与土壤养分状况之间的相关性分析发现，随着海拔、坡向或者植被类型的改变，其林下土壤有机碳及其活性组分与土壤养分的相关性也发生较大的变化。总体而言，岷江上游地区海拔、坡向、土壤自然含水量、植被盖度、凋落物厚度、土壤全N对次生林下土壤有机碳及其组分有重要影响。而AP、AK、C/N对土壤碳变化变化影响较小。 6. 通过不同海拔、坡向以及植被类型之间的综合比较分析发现，土壤微生物碳SMBC和水溶性碳WSOC比TOC和EOC更能敏感地反映出比较敏感的指示林下土壤质量的变化。 In order to reveal seasonal dynamics of soil labile organic carbon under different secondary vegetation, to analyze effect of different vegetation restoration pattern on soil organic carbon and its fractions, and to find the factors influencing changes in soil organic carbon and its fractions, further to estimate those parameters reliability for soil quality monitoring in the process of vegetation restoration. Soils were selected from several plantations, including Picea asperata Pinus tabulaeformis, Pinus armandii and Larix kaempferi and secondary shrub in Dagou Watershed of the upper reach of Minjiang River. The measurement of TOC, SMBC，WSOC and EOC were made, because these parameters can reflect change of soil characteristics. The major results are: 1. There were the lowest soil organic carbon and its labile fractions contents in May. At the time of growth initiation, they increased gradually and reached maximum in September. After that the soil organic carbon content decreased. Because current litter couldn’t be rapidly decomposed, and supplemented into carbon pool, while intrinsic carbon pool experienced decomposition and utilization of growth season, Which led a decrease in soil organic carbon content in November. Average value was 15.48~25.46 g kg-1; average SMBC content was 132.78~476.73mg kg-1.There were higher SMBC content in September and November as compared with other times; Water soluble organic carbon content increased from 51.95 mg kg-1 in May to 77.18 mg kg-1 in November; EOC content was lowest in May y. Average value was 3.74~5.79g kg-1. Differeing from other parameters of carbon fractions, EOC content was higher in July. 2. The content of soil organic carbon and its labile carbon fractions ranked as follows：TOC>EOC >SMBC>WSO，and ratio was about 300：70：5：1. 3. Consider as soil different layers，all of the parameters decreased gradually with increasing soil depth, thus displayed a significant accumulation in the surface layer soil. 4. Correlations coefficient analysis revealed that, TOC significantly correlated with SMBC, WSOC and EOC indicating total storage of organic carbon limited soil labile carbon fractions in great extent. On the other hand, there were significant correlations between SMBC，WSOC and EOC. But these relationships changed with vegetation types and/or environmental conditions. 5. The relationships between soil organic carbon and its labile fractions and soil nutrient traits changed with altitude，slope aspect and vegetations. Therefore our results suggested that altitude，slope aspect，soil natural water content，vegetation coverage, litter thickness and soil total nitrogen play a important role change in soil organic carbon and its fractions in upper reaches of Minjiang River. While AP、AK、C/N slightly influenced soil carbon. 6. Our results, on the other hand suggested that SMBC and WSOC are more sensitive to the change of altitudes, slope aspects, vegetation types than TOC and EOC, thus two parameters may be good index reflecting change of soil quality. These results provide insights into theoretical and technological evidences for the vegetation regeneration restoration and improvement of low-quality and benefit forest in the upper reaches of Minjiang River regions.