Verandah, Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Verandah, Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Side boundary gate, Lahz House, Spring Hill

House beyond with skylight to bathroom visible on left. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Side boundary fence, Lahz House, Spring Hill

House beyond with skylight to bathroom visible on left. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Side boundary fence, Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Street elevation, Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Sitting room, Lahz House, Spring Hill

Kitchen and dining area extension beyond. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Side boundary pathway, Lahz House, Spring Hill

Lahz House on left. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Side boundary fence, Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Verandah alcove, Lahz House, Spring Hill

Double hung window and sculpture in verandah alcove. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Lahz House, Spring Hill

Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Bathroom, Lahz House, Spring Hill

Bathroom vanity with glass skylight above. Winner of the Robin Dods Award RAIA Queensland Chapter 1990

Unexpected role of surface transglutaminase type II in celiac disease

Background & Aims: In celiac disease (CD), transglutaminase type II (TG2) has 2 fundamental roles: (1) as the autoantigen recognized by highly specific autoantibodies and (2) the modifier of pathogenic gliadin T-cell epitopes. It follows that inhibition of TG2 might represent an attractive strategy to curb the toxic action of gliadin. Here we studied the validity of this strategy using the organ culture approach. Methods: Duodenal biopsy specimens from 30 treated patients with CD, 33 untreated patients with CD, and 24 controls were cultured with or without gliadin peptides p31-43, pα-9, and deamidated pα-9 for 20 minutes, 3 hours, and 24 hours. In 31 patients with CD and 16 controls, TG2 inhibitor R283 or anti-TG CUB 7402 or anti-surface TG2 (6B9) mAbs were used in cultures. T84 cells were also cultured with or without peptides with or without TG inhibitors. Mucosal modifications after culture were assessed by immunofluorescence, in situ detection of TG activity, confocal microscopy, and fluorescence-activated cell sorter analysis. Results: The enzymatic inhibition of TG2 only controlled gliadin-specific T-cell activation. The binding of surface TG2 contained gliadin-specific T-cell activation and p31-43-induced actin rearrangement, epithelial phosphorylation, and apoptosis, both in organ cultures and T84 cells. Conclusions: These data indicate a novel and unexpected biological role for surface TG2 in the pathogenesis of CD suggesting a third role for TG2 in CD. These results have a specific impact for celiac disease, with wider implications indicating a novel biologic function of TG2 with possible repercussions in other diseases. © 2005 by the American Gastroenterological Association.

Adenosquamous carcinoma of the esophagogastric junction. Case report

Adenosquamous carcinoma is a rare tumor with coexisting elements of infiltrating squamous cell carcinoma and adenocarcinoma. This tumor is reported to arise in different organs but rarely in the oesophagus. In most cases, it shows highly aggressive biological behaviour with high propensity to regional lymph-node metastasis and poor prognosis. We describe the management of a patient with an aggressive adenosquamous carcinoma of the esophagogastric junction.

Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography

Archaeal replicative DNA polymerase D (PolD) constitute an atypical class of DNA polymerases made of a proofreading exonuclease subunit (DP1) and a larger polymerase catalytic subunit (DP2), both with unknown structures. We have determined the crystal structures of Pyrococcus abyssi DP1 and DP2 at 2.5 and 2.2 angstrom resolution, respectively, revealing a catalytic core strikingly different from all other known DNA polymerases (DNAPs). Rather, the PolD DP2 catalytic core has the same 'double-psi beta-barrel' architecture seen in the RNA polymerase (RNAP) superfamily, which includes multi-subunit transcriptases of all domains of life, homodimeric RNA-silencing pathway RNAPs and atypical viral RNAPs. This finding bridges together, in non-viral world, DNA transcription and DNA replication within the same protein superfamily. This study documents further the complex evolutionary history of the DNA replication apparatus in different domains of life and proposes a classification of all extant DNAPs.