Description of three new species of Hepatozoon (Apicomplexa, Hepatozoidae) from Rattlesnakes (Crotalus durissus terrificus) based on molecular, morphometric and morphologic characters

Hepatozoon spp. are commonly found infecting snakes. Since the latter are parasitized by diverse forms and data in the literature show divergence, we studied Hepatozoon spp. diversity on Crotalus durissus terrificus snakes using both molecular and morphological approaches. Naturally infected animals were employed. Blood was collected, blood smears were prepared and an aliquot was stored at -20. °C for DNA extraction. Five specimens of C. durissus terrificus were selected, each of them infected with one gamont type. Morphological and morphometric analyses of the found gamonts led to their grouping into three populations. For molecular characterization, seven oligonucleotide pairs that amplify distinct regions of rDNA gene were tested by adopting the PCR technique. Only the oligonucleotide pairs HepF300/Hep900 and HEMO1/HEMO2 were efficient in amplifying and distinguishing different isolates of Hepatozoon spp. from snakes. The better results were obtained when both oligonucleotide pairs were used in association. Based on the molecular and morphologic differences, three new species were proposed: Hepatozoon cuestensis sp. nov.; Hepatozoon cevapii sp. nov. and Hepatozoon massardii sp. nov. This is the first description of new Hepatozoon species from snakes, based on molecular characterization and morphological data, in South America. © 2013 Elsevier Inc.

Estudo da rugosidade e ultra-estrutura superficial do esmalte dental humano submetido a diferentes métodos profiláticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Atributos físicos e mecânicos do solo avaliados por processamento digital de imagem e ensaios de rotina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização anatômica quantitativa e composição de óleos essenciais em três estágios foliares de clones de eucalipto e sua relação com a ferrugem

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise micromorfológica e relação com atributos de um solo sob diferentes usos e manejos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento de óvulo, fruto e semente de Xyris (Xyridaceae, Poales)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of grinding with diamond-disc and -bur on the mechanical behavior of a Y-TZP ceramic

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Micromorfologia e anatomia floral das seções neotropicais de Bulbophyllum Thouars (Orchidaceae, Asparagales): considerações taxonômicas e evolutivas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudio descriptivo in vitro de dos agentes blanqueadores de distinta concentración sobre la micromorfología del esmalte bovino

Dental tooth bleaching is a conservative option for the treatment of tooth stains. It is based on the use of hydrogen peroxide as an active agent. Despite its effectiveness to lighten tooth colour, there is concern regarding its use due to the effects it could have over enamel surface. There is scarce evidence on the subject and contradictions exist between different authors. The aim of this study was to compare enamel surface micromorphology after bleaching teeth with different concentrations of hydrogen peroxide solutions. Method: 50 healthy bovine incisors sectioned horizontally at the cemento-enamel junction were prepared. Contents of pulp chamber and tooth surfaces were cleaned. The buccal surface of each tooth was divided vertically, assigning one half to the control group (CG) and the other randomly to: Group 1: 25 samples treated with 15% hydrogen peroxide with nitrogen doped titanium dioxide. Group 2: 25 samples treated with 35% hydrogen peroxide. Square samples (2x2 mm.) were obtained and observed by SEM (magnification of 5.000x and 10.000x). Results: All treated groups showed longitudinal depressions on the surface and increased surface roughness. Conclusions: Tooth bleaching with hydrogen peroxide produces subclinical alterations over bovine enamel surface. 15% hydrogen peroxide bleaching agent produced less micromorphology alteration over bovine enamel surface than the 35% hydrogen peroxide agent.

Effect of long-term storage on nanomechanical and morphological properties of dentin-adhesive interfaces

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A novel gastroretentive floating system for zidovudine, based on calcium-silicate beads

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do efeito do plasma não térmico na resistência adesiva à dentina supericial e profunda

Pós-graduação em Odontologia Restauradora - ICT

Pollination of Anemopaegma album (Bignoniaceae) with focus on floral nectar as the mediator of interactions with mutualistic and antagonistic bees

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Colonização bacteriana na região marginal de restaurações cerâmicas: efeito do método de remoção do cimento e do polimento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Colonização bacteriana na região marginal de restaurações cerâmicas: efeito do método de remoção do cimento e do polimento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)