Effects of the “all-fish” GH (growth hormone) transgene expression on resistance to Ichthyophthirius multifiliis infections in common carp, Cyprinus carpio L.

A study was undertaken on the susceptibility of the F-4 generation of "all-fish" growth hormone transgenic carp, Cyprinus carpio L, against Ichthyophthirius multifiliis infections. When 1-year old, transgenic carp, with non-transgenic carp and non-manipulated carp (controls) were split into three batches, and experimental infections were performed throughout the 3-month period. All 72 fish were successfully infected. It was shown that there was a significant difference (P<0.01) on infection level between transgenics and non-transgenics, and transgenics and controls. It possibly resulted from transgenics that had stronger non-specific immune functions. In addition, fish surface area affected significantly infection level (P<0.001). Carp with larger surface area harboured more parasites for each type of fish, but transgenic with larger surface area than non-transgenics and controls (P<0.01), loaded fewer parasites than others. Besides, the time of infection also greatly influenced (P<0.001) infection level. Results showed that there was a significant decline in parasite infectivity through October to November (P<0.001). It was likely to suggest that there existed senescence resulted in failure of any I. multifiliis isolate maintenance. Significant difference in infectivity between isolate G from grass carp and isolate H from gold fish suggested that different parasite strains may exist. (C) 2009 Elsevier B.V. All rights reserved.

Lagarosiphon major (Ridley) Moss ex Wager (curly water weed)

Curley water weed is a southern African submerged macrophyte that has become a serious water weed in several countries including New Zealand after its introduction by the aquarium industry. It has been recorded in Australia, including Queensland, but is not considered to have established. The chapter describes the ecology and management of this weed. Control of further dispersal is considered critical to its management. It has also been considered for classical biological control and manipulation of grass carp densities has also been studied. Issues relating to the use of herbicides in freshwater systems are also discussed.

Fish production from aquatic weeds

Irrigation in the eleven River Basin Authorities and in particular in the South Chad Irrigation Project and the Baga Polder Project is increasing and demands high yearly investments for aquatic weed control in canals and drains. If the weeds are biologically controlled by the grass carp (Ctenopharyngodon idella Val.), the costs will be turned into profit, particularly when the fish production (resident fish plus grass carp) is harvested and sold for food. The use of irrigation canals and drains for aquaculture in the form of fish polyculture may be a wise step towards increased fish production. This paper highlights the concept of fish production from aquatic weed control and concludes that it is a proven profitable venture in several countries

Panama City Fisheries Resources Office: FY 2005 Annual Report

HIGHLIGHTS FOR FY 2005 1. Assisted with a study to assess hurricane impacts to Gulf sturgeon critical foraging habitat. 2. Documented Gulf sturgeon marine movement and habitat use in the Gulf of Mexico. 3. Documented Gulf sturgeon spawning with the collection of fertilized eggs in the Apalachicola River, Florida. 4. Documented Gulf sturgeon spawning with the collection of fertilized eggs in the Yellow River, Florida. 5. Assisted with benthic invertebrate survey at Gulf sturgeon marine foraging grounds. 6. Implemented Gulf Striped Bass Restoration Plan by coordinating the 22nd Annual Morone Workshop, leading the technical committee, transporting broodfish, and coordinating the stocking on the Apalachicola-Chattahoochee-Flint (ACF) river system. 7. Over 87,000 Phase II Gulf striped bass were marked with sequential coded wire tags and stocked in the Apalachicola River. Post-stocking evaluations were conducted at 45 sites in the fall and spring and 8 thermal refuges in the summer. 8. Completed fishery surveys on 4 ponds on Eglin AFB totaling 53 acres, and completed a report with recommendations for future recreational fishery needs. 9. Completed final report for aquatic monitoring at Eglin AFB from 1999 to 2004. 10. Completed a field collection of the endangered Okaloosa darter to be incorporated into a status review to be completed in FY06. 11. Provided technical assistance to the Region 4 National Wildlife Refuge (NWR) program on changes to the fishery conservation targets for the region. Also provided technical assistance to four NWRs (i.e., Okefenokee NWR, Banks Lake NWR, St. Vincent NWR, and St. Marks NWR) relative to hurricanes and recreational fishing. 12. A draft mussel sampling protocol was tested in wadeable streams in Northwest Florida and southwest Georgia, and an associated field guide, poster, and Freshwater Mussel Survey Protocol and Identification workshop were completed in FY05. 13. Implemented recovery plan and candidate conservation actions for 14 listed and candidate freshwater mussels in the Northeast Gulf Watersheds. 14. Initiated or completed multiple stream restoration and watershed management projects. A total of 7.5 stream miles were restored for stream fishes, and 11 miles of coastline were enhanced for sea turtle lighting. A total of 630 acres of wetlands and 2,401 acres of understory habitat were restored. 15. Conducted a watershed assessment to develop a threats analysis for prioritizing restoration, protection, and enhancement to natural resources of Spring Creek, Georgia and Canoe Creek, Florida. 16. Continued the formation of an Unpaved Road Interagency Team of Federal, State, and local agencies in Northwest Florida to promote stream protection and restoration from unpaved road sediment runoff. Began the development of a technical committee agreement. 17. Conducted Alabama Unpaved Road Inventory within the Northeast Gulf Ecosystem. Data collection will be completed during FY06. 18. Finalized the development of two North Florida hydrophysiographic regional curves for use by the Florida Department of Transportation (DOT) and others involved with stream restoration and protection. Initiated the development of the Alabama Coastal Plain Riparian Reference Reach and Regional Curves for use by the Alabama Department of Environmental Management (ADEM). 19. Provided technical assistance in collecting data, analysis, and thesis formulation with Troy University, Alabama, to identify the influence of large woody debris in southeastern coastal plain streams. 20. Completed pre- and post-restoration fish community monitoring at several restoration projects including Big Escambia Creek, Magnolia Creek, and Oyster Lake, Florida. 21. Established a watershed partnership for the Chipola River in Alabama and Florida and expanded development and participation in the Spring Creek Watershed Partnership, Georgia. 22. Continued to identify barriers which inhibit the movement of aquatic species within the Northeast Gulf Ecoregion. 23. Completed a report on road crossing structures in Okaloosa darter streams to guide the closure/repair/maintenance of roads to contribute to recovery of the endangered species. In cooperation with Three Rivers RC&D Council, fish passage sites identified in the report were prioritized for restoration. 24. Monitored Aquatic Nuisance Species in the Apalachicola River and tested the sterility of exotic grass carp. 25. Multiple outreach projects were completed to detail aquatic resources conservation needs and opportunities. Participated in National Fishing Week event, several festivals, and school outreach.

A computer software package for assessing and managing risks posed by experiments with genetically modified fish and shellfish

Assessment and management of risk is needed for sustainable use of genetically modified aquatic organisms (aquatic GMOs). A computer software package for safely conducting research with genetically modified fish and shellfish is described. By answering a series of questions about the organism and the accessible aquatic ecosystem, a researcher or oversight authority can either identify specific risks or conclude that there is a specific reason for safety of the experiment. Risk assessment protocols with examples involving transgenic coho salmon, triploid grass carp and hybrid tilapia are described. In case a specific risk is identified, the user is led to consider risk management measures, involving culture methods, facilities design and operations management, to minimize the risk. Key features of the software are its user-friendly organization; easy access to explanatory text, literature citations and glossary; and automated completion of a worksheet. Documented completion of the Performance Standards can facilitate approval of a well designed experiment by oversight authorities.

Mono- and polyculture of silver barb (Puntius gonionotus) in deepwater rice systems in Bangladesh

Experiments with fish enclosures were conducted at the Deepwater Rice Farming Systems Research Site at Shuvullah, Mirzapur, Bangladesh. The objective was to study the performance of silver barb (Puntius gonionotus) called Thai sharputi or rajputi in Bangladesh in mono-and-polyculture with grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), catla (Catla catla) and rohu (Labeo rohita). Each enclosure measured 21 m x 21 m with an approximate net height of 3.5 m. The stocking densities per cubic meter were 1 fingerling for Thai sharputi monoculture (enclosure 1), and 2 fingerlings for the polyculture systems (enclosure 2 and 3). The species ratio for enclosure 2 was 0.37:0.27:0.02:0.34 (grass carp:Thai sharputi:common carp:catla) and for enclosure 3, 0.4:0.4:0.2 (catla:rohu:Thai sharputi). In monoculture (enclosure 1), Thai sharputi performed well. This relatively good production was mainly attributed to the use of appropriately sized fingerlings and rapid growth from consumption of an abundant supply of azolla in addition to feed given. For the polyculture in enclosure 2, the average weight gain of common carp was the highest (673 g) followed by grass carp (475 g) and Thai sharputi (286 g). For the polyculture in enclosure 3, the length and weight gains for Thai sharputi were almost the same as for the monoculture.

Regional Fisheries Advisory Committee Meeting 15th October, 1984

This is the report from the Regional Fisheries Advisory Committee meeting, which was held on the 15th October, 1984. The report contains sections on Grass Carp Field Trials in the Lancaster Canal, liaisons with Sea Fisheries Committees, Rivers Leven and Crake migratory fish stocking, and migratory fish stocking policy. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Regional Fisheries Advisory Committee 4th February, 1985

This is the report from the Regional Fisheries Advisory Committee meeting, which was held on the 4th February, 1985. The report contains information on fishing licence duties (1986), Lead and Swans, fyke Nets, Grass Carp Field Trials in the Lancaster Canal and the total available residual chlorine (TARC) in the River Lune. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Increasing farm income by introducing fish culture in deepwater rice environment

Fish culture in deep-water-rice (DWR) environment using net pen and polder systems was evaluated. In net pen rohu and Thai silver barb were cultured, whereas a 5-species combination (rohu, mrigal, common carp, grass carp and Thai silver barb) were cultured with BR3 rice variety and DWR. Boro-fish production system produced 2.8 t/ha of fish and 7.33 t/ha of rice in polder system with 5-species combinations.

A Preliminary survey of 21 Ceylon lakes. 3. Parasites and predators, food of fish, and marginal fauna

Three nematodes, two acanthocephalans and three cestodes were collected by dissecting 15 species of fish. Of these parasites only Hedruris sp. a larval nematode is responsible directly for the death of fish, since it is found in predatory species a reduction of these might prove of considerable consequence to fish, production. Bothriocephalus gowleonensis, a cestode probably introduced from China with Chinese carp, was recorded in an indigenous carp Puntius sarana. This parasite causes heavy mortality in grass carp in China and is a potential danger to local species. A single crustacean parasite was recorded. It is likely that they are widespread. No protozoa were recorded but there can be little doubt that they occur in local freshwater species and may be of considerable importance in fisheries. Several predators (insects, fish and other vertebrates) were recorded and are doubtless of considerable importance. However, the role of parasites and of predators (especially fishes) in relation to the productivity of fisheries can hardly be assessed at present and needs further study. A study of the fauna found at the shallow edges of 21 irrigation reservoirs showed an abundance of fauna in the low-country reservoirs whilst the up-country reservoirs had a poor fauna. The fauna showed insects, crustacea and mollusca in this order of abundance. The presence of insect and molluscs feeding fish in our fauna shows that these rich sources of food are being utilised.

Induced spawning of two major species of Chinese carps, Ctenopharyngodon idellus and Aristychthys nobilis in Sri Lanka

Induced spawning of grass carp (Ctenopharyngodon idellus) and bighead carp (Aristychthys nobilis) was carried out successfully in Sri Lanka for the first time in 1977 and 1978, respectively. This article describes the techniques involved in induced spawning in Sri Lanka at the Freshwater Fish Breeding and Experimental Station in Udawalawa.

Polyculture of freshwater prawn, Macrobrachium rosenbergii, de Man with carps: effects of prawn stocking density

Effects of three stocking densities, viz., 35, 50 and 65/decimal (1 decimal = 40.48 m2) of juvenile freshwater prawn (Macrobrachium rosenbergii) on prawn and fish production were tested in a polycuture system with silver carp (Hypophthalmichthys molitrix), catla (Catla catla), Grass carp (Ctenophmyngodon idella) and silver barb (Barbodes gonionotus). The fish stocking density was 19/decimal with the species combination of silver carp-10, catlac-6, grass carp-1 and silver barb-2. In a 8-month culture period, the prawn yield 423 ± 144 kg/ha was significantly lower (P< 0.5) with the prawn stocking density of 35/decimal than that of 548 ± 178 kg/ha and 662 ± 243 kg/ha with 50 and 65/decimal respectively. The fish production (1844-1891 kg/ha) did not differ significantly (p <0.05) among the three treatments indicating that prawn stocking densities had no influence on fish yield. The lower mean harvest weight (62 g) and survival rate (67 g) and higher yield (2.67 kg/decimal) with the highest stocking rate of prawn reveals that as density was increased, prawn survival and individual weight at harvest decreased but total yield increased.

Culture potentials of bata, Labeo bata under semi-intensive management system

Small indigenous fish species (SIS) provide food, nutrition, subsistence and supplemental income to a great majority of the people particularly the poor and disadvantaged people of Bangladesh. To date nobody explored the possibilities of culturing these species in combination with the indigenous major carps viz. catla, Catla catla; rohu, Labeo rohita, and mrigal Cirrhinus cirrhosus and grass carp, Ctenopharyngodon idellus. An experiment on the polyculture of carps with a popular SIS, bata (Labeo bata) was carried out to evaluate the production performance of carp-SIS culture in on-farm condition during 15 March to 15 September 2003. Three treatments each having three replications with different stocking densities of bata were tested keeping the carp species combination and stocking density similar in all the treatments viz. treatment 1 (T1), bata (5,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); treatment 2 (T2), bata (7,500/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); and treatment 3 (T3), bata (1 0,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha). After six months rearing, the production obtained were 2,466±77, 2,395±85 and 2,074±72 kg/ha from T1, T2 and T3, respectively. The highest production was obtained from T1, where the stocking density of bata was the minimum (5,000/ha) while the lowest production was obtained from T1 [sic], where the stocking density of bata was maximum (10,000/ha). Significant difference (P<0.05) exists in the production levels as obtained from different treatments. The contribution of bata to total production was 10.31%, 13.96% and 14.38% in case of T1, T2 and T3, respectively.

On farm trial of Bangladesh Fisheries Research Institute (BFRI) evolved two aquaculture technologies

Two BFRI evolved aquaculture technologies - integrated rice fish farming and carp polyculture with over-wintered fingerlings under different stocking densities were tested during 2003-04. The study was coordinated with two local NGOs namely NICHAITA and JNDP, Muktagacha, Mymensingh. Integrated rice fish farming technology was demonstrated in 9 plots each having an area between 60-100 dec. during boro season. Fifteen days after transplantation of rice seedlings, fingerlings of rajpunti (Barbodes gonionotus) of 7-10 g of individual weight were stocked in the rice fields at the density of 3,000 (T1), 3,750 (T2) and 4,500/ha (T3). The corresponding final weight of fish after three and half months in treatments 1, 2 and 3 were 110±14.21, 101±16.55 and 86±22.28 g, respectively. The mean weight of fish in treatments 1 and 2 was significantly higher than treatment 3. Fish production obtained from treatments 1, 2 and 3 were 218.16±18.29, 239.70±25.11 and 236±24.66 kg/ha, respectively. On-farm demonstrations of carp polyculture using over-wintered fingerlings rohu (25-28 g), catla (24-26 g), mrigal (21-26 g) and grass carp (20-24 g) under different stocking densities were undertaken in nine earthen ponds (1,200-1,600 square meters) for a period of six months at three different stocking densities. The stocking densities of treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) were 2,000, 3,000 and 4,000/ha, respectively. Fish were fed with rice bran and mustard oil cake (3:1). Soft green grass and banana leaves were provided mainly for grass carp. At harvest, the production obtained in treatments 1, 2 and 3 were 2,325±74.75, 2,620±49.66 and 2,982± 171.52 kg/ha, respectively. The results demonstrated higher growth of fish in treatment 1 than those of treatments 2 and 3. However, treatment 3 contributed relatively higher production than those of treatments 1 and 2, whereas, highest net benefit was received from treatment 2.

Shifting rice farming to fish culture in some selected areas of Mymensingh, Bangladesh: the process, conflicts and impacts

The evolutionary process of converting low-lying paddy fields into fish farms and its impact on agrarian communities in some selected areas of Mymensingh district were studied. This study was conducted through participatory rural appraisal (PRA) covering 12 villages from each of selected upazillas viz. Fulpur and Haluaghat of Mymensing [sic] district. A total of 12 PRA sessions were conducted where 90 farmers participated during 29 July to 26 August 2004. It is seen that the use of low-lying paddy fields was mostly confined to Broadcast Aman (B. Aman) rice production until 1960s. With the introduction of modern rice farming technology, the farmers started to produce Boro rice in Rabi season and B. Aman rice in Kharif season. With the passage of time, aquaculture technologies have been evolved and the farmers realized that fish farming is more profitable than rice cultivation, and then they started to utilize their paddy fields for alternate rice-fish farming and rice-cum-fish farming. Now a days, aquaculture based crop production system is in practice in more than 25% of the low-lying paddy fields. Conversion of rice fields in to fish ponds has brought up a change in the livelihood patterns of the rural farmers. The areas where the farmers involved themselves in the new production systems were fingerling collection, transportation and marketing of fry and fingerlings. During 1960s to 1970s, a few people used to culture fish in the permanent ponds for their own consumption, the species produced were rohu, catla, mrigal, ghainna, long whiskered catfish, freshwater shark (boal), snake head (shol) etc. Small fishes like climbing perch, stinging catfish, walking catfish, barb, minnows etc. were available in the rice fields during monsoon season. In 1980s to mid 1990s, some rice fields were converted into fish ponds and the people started to produce fish for commercial purposes. When rice-fish farming became profitable, a large number of people started converting their rice fields in to rice-fish culture ponds. Culture of some exotic fishes like silver carp, tilapia, grass carp, silver barb etc. also started in the paddy fields. Higher income from fish farming contributed positively in improving the housing, sanitation and education system in the study areas. It is seen that the medium and medium high lands were only used for alternate rice fish farming. The net income was high in any fish based cropping system that motivated the farmers to introduce fish based cropping system in the low-lying inland areas. As a result, the regional as well as communal income disparities occurred. However, the extraction of ground water became common during the dry period as the water was used for both rice and fish farming. Mass conversion of paddy fields into rice-fish culture ponds caused water logging in the study areas. In most cases, the participated farmers mentioned that they could be easily benefited by producing fish with T. Aman or only fish during the monsoon season. They agreed that this was an impressive technology to them and they could generate employment opportunities throughout the year. Finally, the social, economic and technical problems which are acting as constraints to rapid expansion of fish production system were reported from the interviewee.