Growth and survival of Clarias batrachus (Lin.) larvae fed on formulated diets

An experiment was conducted for a period of 28 days using 7-day old C. batrachus larvae of initial total length (±S.D.) of 7.4 ± 0.49 mm and weight (±S.D.) of 2.8 ± 0.75 mg. Five test diets viz. diets I, II, III, IV and V were prepared. Diet I was formulated using 30% fish meal (FM), 20% soybean meal (SM) and 20% Baker's yeast (BY), diet III using 15% FM, 20% SM, 15% cotton seed meal and 30% BY and diet IV using 20% FM, 30% BY and 30% powdered milk. The remaining two diets (diets II and V) were prepared using minced clam meat (96%) and BY (90%) respectively as the sole source of protein. Three replications were used for each treatment. The larvae fed on diet II exhibited significantly (P<0.05) better length gain and specific growth rate (SGR) than those of the larvae fed on diet III and there was no significant difference among the length gain and SGR of the larvae fed on diet I,II,IV and V. The larvae fed on diet II also showed significantly better weight gain and survival than those of the larvae fed on other diets. There was no significant difference between the condition factor of the larvae fed on diet II and IV. However, the larvae fed on diet II showed better condition factor than those of the larvae fed on other diets.

Effects of duckweed (Lemna minor) as dietary fishmeal substitute for silver barb (Barbodes gonionotus Bleeker)

A 60-day long growth trial was conducted to evaluate the suitability of duckweed Lemna minor as dietary fish meal substitute for silver barb (Borbodes gonionotus Bleeker). Five iso-nitrogenous diets were formulated to contain 35% protein and each treatment had three replicates with 15 fish in each aquarium with a mean initial weight of 1.5 ± 0.2 g. Duckweed was used in the experiment to replace 10, 20, 30 and 35% of the dietary fish meal in diet 2, 3, 4 and 5 respectively. Fish meal was used as the sole source of protein in control diet (Diet 1). Fish were fed three times daily at satiation level. In terms of growth, food conversion and protein utilization, the control diet and diet containing 17.07% duckweed showed the best (P<0.05) performance followed by diets containing 34.14%, 51.21% and 59.24% duckweed. Fish fed diets containing higher levels of duckweed had higher carcass moisture and lower lipid content compared to the control diet. Histopathological examination revealed abnormalities in the liver of fish fed diets containing higher inclusion of duckweed. It was noted that 10% of the dietary fish meal protein could be replaced by duckweed (L. minor) in the diet of silver barb (B. gonionotus).

Nutrient digestibility coefficients of diets with varying energy to protein ratio for Japanese flounder, Paralichthys olivaceus

A laboratory trial was conducted in a sea water recirculatory system to study the nutrient digestibility coefficients of diets with varying energy to protein ratios in Japanese flounder Paralicthys olivaceus. Six different experimental diets with two protein levels (45 and 55%) having six different energy to protein ratio of 87, 90, 94, 107, 110 and 114 were formulated using white fish meal and casein as protein sources. The results of the study showed that the apparent protein digestibility (APD) value ranged between 90.59 to 91.61% and there were no significant differences (P>0.05) between the APD values of diets 1, 2, 3, 4 and 6. The apparent lipid digestibility (ALD) values of diets ranged between 88.24 to 90.18%. The apparent energy digestibility (AED) values ranged between 80.55 to 87.52% with diet 3 producing significantly the highest AED value. In general, except in diet 1 the ALD and AED values increased with the increase of dietary lipid at both protein levels. The results of the present investigation indicated that Japanese flounder can efficiently digest the dietary nutrients at varying energy to protein ratios.

Effects of animal and plant protein diets on growth of Indian major carp (Labeo rohita Ham.) fingerlings

An experiment was conducted with Labeo rohita fingerlings in an indoor static fish rearing water system of glass made aquaria. Five experimental diets A, B, C, D and E were formulated containing 33% dietary protein level in five treatments each having two replicates containing 12 fingerlings of mean total initial weight of 13.00±0.2g. Sixty days of feeding trial in this experiment showed that fish fed on diet 'A' containing fish meal and diet 'E' containing mixed plant sources protein had significantly highest and lowest growth respectively. However, no significant difference of growth was found in fish fed on diets C and D containing meat and bone meal, and mix of animal protein source diets respectively. The result showed that the apparent protein digestibility (APD) of diets 'A' and 'E' had significantly best and least values respectively. Food conversion ratio (FCR) and protein efficiency ratio (PER) ranged between 1.37 to 2.17 and 1.38 to 2.18 respectively. On the basis of observed FCR and PER diets 'A' and 'E' produced significantly highest and lowest growth respectively.

Effects of physiological doses of vitamin D sub(3) (Cholecalciferol) on induced molting and growth in giant freshwater prawn, Macrobrachium rosenbergii (de Man)

Effects of three different doses of vitamin D sub(3) on molting, growth, and calcium and phosphate composition of tissue and molt during the grow-out of the giant freshwater prawn Macrobrachium rosenbergii (average weight 10.56 ± 0.20 g), obtained from a grow-out pond, were studied. Intramuscular doses of vitamin D sub(3) (100, 500 and 2000 IU/kg body weight) were given on the 1st, 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 20th, 25th and 30th days. All the experimental animals were fed with a basal diet containing fish meal, shrimp meal, wheat flour, groundnut de-oiled cake, soybean meal and wheat bran at 3% of the body weight. The numbers of molts were recorded as 20±0.50, 29±1.16, 51±1.87, and 30±1.60 in control, 100, 500 and 2000 IU/kg body weight physiological doses, respectively. Maximum growth was recorded in prawns given 500 IU/ kg dose. Survival was between 58.33 ± 9.13 and 77.77 ± 8.61%. The ash content and calcium level increased significantly (p<0.05) and recorded the highest values in 500 IU/kg physiological dose. However, the inorganic phosphate (P sub(i)) content recorded the highest values in tissue in 2000 IU/kg dose (p<0.05, F = 50.60613). There is no significant difference in calcium contents (p>0.05) in both tissue and molt at 500 and 2000 IU/kg doses. It was found that a higher physiological dose (2000 IU/kg) of vitamin D sub(3) increased the rate of mortality. Results have shown that vitamin D sub(3) has a positive impact on the growth and survival of M. rosenbergii and it interferes with the metabolism of Ca and P sub(i), in tissue, and alters molting frequency. Results on physiological dose suggest an alternative and effective dietary supplementation method of vitamin D sub(3) in the grow-out phase of M. rosenbergii.

Effect of farm made feeds on polyculture of shrimp (Penaeus monodon) and three brackishwater finfish species

A study was conducted to evaluate the effects of feed made from locally available ingredients on polyculture of shrimp and three brackishwater finfish species. Hatchery produced post-larvae (PL) of shrimp Penaeus monodon (0.005g) were stocked at the rate of 15,000 PLs/ha. Brackishwater finfish species Liza parsia, Mugil cephalus and Rhinomugil corsula of 0.63-1.4lg collected from local rivers were stocked at the rate 8,000, 1,000 and 2,000/ha, respectively in four treatments. Shrimp and finfishes were fed four different experimental diets composed of fish meal, mustard oil cake, rice bran, oyster shell power and vitamin premixes at the rate of 3-5% estimated crop/day for 195 days. Among four treatments, P. monodon showed comparative better growth in T4 and T3. Finfish L. parsia showed its better performance in treatment T2. Species M. cephalus and R. corsula showed insignificant production. P. monodon showed better growth with diet of fish meal and mustard oil cake@ 28.84 and 33.65%, respectively in T 3 and 19.22 and 43.27%, respectively in treatment T4.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Influence of stocking density on the culture potential of freshwater catfish Pangasius pangasius in pond

Influence of stocking density on the production of freshwater catfish Pangasius pangasius with formulated feed in ponds was studied. The fish fry were collected from the Meghna river near Chandpur which is a natural breeding ground of this fish. Three stocking densities chosen during this experiment were 5000, 8000 and 11000 fry/ha with an initial weight of 42.57 ±2.51 g. The formulated feed was prepared in the laboratory which contained 45% fish meal, 30% mustard oil cake, 15% wheat bran and 10% rice bran to supply 40.48% protein in feed. The growth of P. pangasius in terms of net weight gain was 409.49, 266.49 and 236.37 g at the 5000/ha, 8000/ha and 11000/ha stocking densities respectively, which was statistically significant (p<0.05). High food conversion ratio (FCR) was also observed during this research study which ranged between 7.06 to 7.72. A production of 2.6 tons/ha at the stocking density of 11000/ha; 2.13 tons/ha at the stocking density of 8000/ha and 2.04 tons/ha at the stocking density of 5000/ha was obtained, while the growth of individual fish at the end of experiment was in opposite order. The result of this experiment indicates that stocking density has significant influence on the culture potential of freshwater catfish P.pangasius.

Development of low cost feed using local ingredients for culture of freshwater prawn, Macrobrachium rosenbergii in ponds

Two experiments were conducted to formulate and evaluate test diets using locally available ingredients to find out suitable diets for mono and polyculture of Macrobrachium rosenbergii in ponds. The first experiment was conducted from 1 July 2003 to 29 September 2003 in 12 experimental ponds each measuring 30 square meters behind the Fisheries Faculty Building, BAU campus, Mymensingh. Three experimental diets containing 30% protein were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran and assigned to treatments 1, 2 and 3, respectively. A special shrimp feed (Starter-II) from Saudi-Bangla Fish Feed Ltd. was assigned to treatment 4 (Control). Each treatment had three replications. Juveniles of M. rosenbergii (2.90±0.81g) were stocked at the rate of 4/square meter. Prawns were fed three times daily at the rate of 15% of their body weight at the beginning, which was gradually reduced to 10% and 5% for the last two months. There was no significant (P>0.05) difference between the weight gains of prawns fed diets 1 and 4 (control), but they were significantly (P<0.05) higher than those of diets 2 and 3. The FCR values of diets ranged between 2.61 to 3.36 with diets 1 and 4 showing significantly (P<0.05) lower FCR values. The survival rate of prawns ranged between 68 to 78% with prawns fed diets 1 and 4 showing significantly higher survival rate. The production of prawn ranged from 921 to 1,428 kg/ha/90 days and diet 1 gave the highest production. Treatment 1 gave the highest net profit ofTk. 161,980/ha/90 days. The second experiment was conducted from December, 2003 to April, 2004 to see the growth of over wintered M. rosenbergii juveniles in polyculture with indigenous major carps (catla and rohu) in ponds using formulated diets. Three isoenergetic experimental diets formulated using fish meal, meat and bone meal, mustard oil cake, rice bran, wheat bran and molasses, and a shrimp feed 'Golda special feed' from Saudi-Bangla Fish Feed Ltd. were assigned to treatments 1, 2, 3 and 4 (control), respectively, each treatment had two replications. Eight experimental ponds each measuring 80 square meters in the Field Laboratory Complex of the Faculty of Fisheries, BAU campus, Mymensingh were used. The mean initial weights of M. rosenbergii, catla and rohu were 1.60±0.01, 30.0±0.09 and 25.0±0.08 g, respectively. A total of 160 fish and prawn (20,000/ha) were stocked in each pond at the ratio of 2:1:1 (prawn: catla: rohu). Fish were fed at the rate of 3-5% of their body weight. Prawns fed diet 1, 2 and 4 showed higher weight gains compared to diet 3. The weight gain of catla was significantly higher in T1 while in case of rohu was higher in T1 and T4, respectively. There were no significant differences (P>0.05) in the survival rate of fish as obtained from different treatments. The overall total fish production ranged from 2196 to 2679 kg/ha/5 months. The highest production and the highest profit (Tk. 56,531/ha/5 months) was obtained from T1 and the lowest (Tk. 24,932/ha/5 months) from T4.

Effect of feeding frequency on the growth of common carp (Cyprinus carpio L.) fry

A laboratory-feeding trail was conducted for 45 days with fry of common carp Cyprinus carpio L. (0.45±0.03g) in aquaria in a static indoor fish rearing system. The fry were fed on a pelleted diet containing 33% crude protein having fishmeal as major protein source. The fish fry in five treatments A, B, C, D, and E, each with two replicates were fed on 5% daily ration divided into different feeding frequencies of 2, 3, 4, 5 and 6 times a day respectively in order to observe the growth performance. Each replicate contained 15 fry having total initial weight of 6.87±0.31g. At the end of the feeding trial, significantly different and higher (p<0.05) growth response was observed in treatment C having a feeding frequencies of 4 times a day. Significantly the highest and the lowest percent growth of 334.30 and 218.91% were observed in fish fed on the diet (Treatment C) with 4 times and (Treatment A) 2 times feeding frequencies per day, respectively. Food conversion ratio (FCR) of 1.78 was significantly higher (pfish fed on the diet having 2 times feeding frequencies whereas, the least value of 1.22 was obtained in fish fed on the diet with 4 times daily feeding. Protein efficiency ratio (PER) ranged from 1.68 in fish in treatment A having a feeding frequencies of 2 times per day to 2.48 in fish in treatment C fed on the diet with 4 times feeding frequencies. Other growth parameters viz, specific growth rate (SGR), apparent protein digestibility (ADP) were also higher in treatment C than the other treatments. The results of the present study demonstrated that the growth performance of C. carpio was the best at 4 times feeding in a day using 33% dietary protein containing fish meal as major protein source.

Input-output relationship and economics of pangas monoculture and carp-pangas polyculture in two districts of Bangladesh

An attempt was made to study the input-output relationships and economics of pangas monoculture and carp-pangas polyculture in Bangladesh. By analyzing the data collected from 50 pangas farms and 55 carp-pangas farms, the study has investigated the production systems of two technologies and the effects of fingerling stocking and applications of feed and fertilizer on fisheries income. The data were collected from the fishermen of Trishal and Bhaluka of Mymensingh district, and Kahaloo and Adamdighee of Bogra district during 2001-02. For pangas monoculture, the stocking density was 31,561 per ha while it was 55,017 per ha in carp-pangas polyculture. Most of the farmers used urea, TSP and lime before stocking. Rice and wheat bran happened to be the most common feed ingredients for both types of culture in general. Other important ingredients used were mustard oil-cakes, rice polish, wheat flour, fish meal, bone meal, soybean meal and poultry litter. In terms of quantities, rice bran and wheat bran dominated the farmers list. Rice and wheat bran together constituted about 60% of all studied feeds. Feed cost constituted 59.13% of total costs for pangas monoculture and 67.44% for carp-pangas polyculture. Per ha productions of pangas and carp-pangas in a single culture cycle were 15,508 kg and 19,745 kg, respectively. Per ha gross profits were estimated to be Tk 310,311 and Tk 464,418 for pangas monoculture and carp-pangas polyculture, respectively. Net profit appeared to be Tk 264,216 per ha for pangas monoculture and Tk 416,509 per ha for carp-pangas polyculture. The BCRs calculated were 1.46 and 1.68 for monoculture and polyculture, respectively. The break-even costs per kg of fish were estimated at Tk 36.93 for pangas and Tk 30.93 for mixed species which was much lower than the prices the producers received. Break-even productions were estimated at 10,702 kg per ha for pangas monoculture and 11,784 kg per ha for carp-pangas polyculture. Fingerling and feed cost, and pond size significantly explained the variation of income from pangas monoculture. These factors have significantly influenced the income from the crop. Functional analysis shows that 1% increase in the feed cost might increase 0.51% of pangas income and 0.41% in carp-pangas income. No other inputs had shown this much of responses to increasing income from a fish.

HACCP based management system for improving quality of freshwater giant prawn, Macrobrachium rosenbergii

The study was conducted on the present status of HACCP based quality management system of golda, Macrobrachium rosenbergii farms in Fulpur region of Mymensingh. Information was collected on general condition of farms, culture systems and post-harvest quality management. In almost all farms, there is no or inadequate infrastructure facilities such as, road access, electric supply, telecommunications, ice, feed storage facility, vehicle for golda transportation, washing and toilet facilities. The problems associated with sanitation and hygiene was: widespread use of cow dung, poultry manure and construction of open toilet within the vicinity of prawn culture pond. Different grades of commercially available and locally prepared feeds were used for golda culture in the pond. Golda post-larvae (PL) of 40-50 days old were stocked with carp species. The price of golda PL ranged from Tk. 1.00 to Tk. 1.25/piece. The pond size varied from 50 decimal (0.2 ha) to 2.5 acre (1.0 ha) with an average depth of 2-2.5 m. The culture period of golda varied from April-May to November-December and survival rate ranged between 75 and 80%. Production of golda varied from 250-500 kg/acre (625-1,250 kg/ha). Harvested golda were transported to city market within 4 h. Two size grading were generally followed during pricing, e.g. Tk. 500 to 550/kg for >100 g size and Tk. 300/kg for <100 g size. The cost-benefit ratio was found to remain around 1:1.25 depending on availability of PL. Water quality parameters such as, water temperature, pH, dissolved oxygen, total alkalinity and chlorophyll a in five golda farms in Fulpur region were monitored. Water temperature ranged from 29°C to 33°C, dissolved oxygen from 2.28 to 4.13 mg/l, pH between 6.65 and 7.94, alkalinity from 44 to 70 mg/l and chlorophyll a concentration from 61.88 to 102.34 µg/l in the five investigated ponds. The Aerobic Plate Count (APC) of the water sample was within the range of 2.0x10^6 - 2.96x10^7 CFU/ml and of soil samples within the range of 6.9x10^6 - 7.73x10^6 CFU/g. Streptococcus sp., Bacillus sp., Escherichia coli, Staphylococcus sp., Pseudomonas sp. and Salmonella sp. were isolated from pond water and sediment. Different feed samples used for golda was analyzed for proximate composition. Moisture content ranged around 14.14-21.22%, crude protein 20.55-44.1%, lipid 4.67-12.54% and ash 9.7-27.69%. The TVB-N values and peroxide values of feeds used as starter, grower and fish meal were found within the acceptable ranges and samples were free from pathogenic organisms. A training was organized for the golda farmers on HACCP, water quality and post-harvest quality management of prawn.

Utilization of frog waste

Commercial frog waste samples have been converted into meals by cooking at 0.7 kg/sq. cm for 30 min, draining off the stick water and drying the press cake either in the sun, tunnel dryer under controlled conditions or hot air oven. Yield of the meal varied between 18.6 to 21.5% of the fresh frog waste. Chemical analyses of the meals have shown that the meals prepared from frog waste conform to standards prescribed for fish meal and livestock feed and can therefore be used for supplementation of poultry/animal feed.

Formulation, stability and keeping quality of three pelleted feeds used in carp culture

Three dry pelleted feeds incorporating fish meal, fish silage or a mixture of colocasia leaf powder and fish meal were formulated for use in carp culture. The diets formulated were tested for water stability and also for changes in their quality parameters over storage of three months. The different pellets showed satisfactory water stability. The variations recorded in the proximate composition during the period of storage did not bring about any drastic change in the overall keeping quality of the feeds. Therefore, the three formulated feeds are considered suitable for use in the culture of carps.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon , were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not signficantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.