Interactions between adult migratory striped bass (Morone saxatilis) and their prey during winter off the Virginia and North Carolina Atlantic coast from 1994 through 2007

The migratory population of striped bass (Morone saxatilis) (>400 mm total length[TL]) spends winter in the Atlantic Ocean off the Virginia and North Carolina coasts of the United States. Information on trophic dynamics for these large adults during winter is limited. Feeding habits and prey were described from stomach contents of 1154 striped bass ranging from 373 to 1250 mm TL, collected from trawls during winters of 1994-96, 2000, and 2002-03, and from the recreational fishery during 2005-07. Nineteen prey species were present in the diet. Overall, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli) dominated the diet by boimass (67.9%) and numerically (68.6%). The percent biomass of Atlantic menhaden during 1994-2003 to 87.0% during 2005-07. Demersal fish species such as Atlantic croaker (Micropogonias undulatus) and spot (Leiostomus xanthurus) represented <15% of the diet biomass, whereas alosines (Alosa spp.) were rarely observed. Invertebrates were least important, contributing <1.0% by biomass and numerically. Striped bass are capable of feeding on a wide range of prey sizes (2% to 43% of their total length). This study outlines the importance of clupeoid fishes to striped bass winter production and also shows that predation may be exerting pressure on one of their dominant prey, the Atlantic menhaden.

El arte en la enfermería: referencias visuales de la Metodología Enfermera Aplicadas al P.A.E., según el modelo de Virginia Henderson

[ES]Con este trabajo se procedió a determinar la fidelidad con la que la cinematografía española recoge la esencia de la asistencia de enfermería teniendo en cuenta el modelo de Virginia Henderson. Para llevar a cabo la investigación del papel de enfermería se analizaron siete película con origen español cuya filmación transcurre en centros sanitarios. Tras analizar los largometrajes se llegó a la conclusión de que la cinematografía española recoge con fidelidad la esencia de las necesidades básicas de Virginia Henderson.

Estimating consumption rates of juvenile sandbar sharks (Carcharhinus plumbeus) in Chesapeake Bay, Virginia, using a bioenergetics model*

Using a bioenergetics model, we estimated daily ration and seasonal prey consumption rates for six age classes of juvenile sandbar sharks (Carcharhinus plumbeus) in the lower Chesapeake Bay summer nursery area. The model, incorporating habitat and species-specific data on growth rates, metabolic rate, diet composition, water temperature (range 16.8−27.9°C), and population structure, predicted mean daily rations between 2.17 ±0.03 (age-0) and 1.30 ±0.02 (age-5) % body mass/day. These daily rations are higher than earlier predictions for sandbar sharks but are comparable to those for ecologically similar shark species. The total nursery population of sandbar sharks was predicted to consume ~124,000 kg of prey during their 4.5 month stay in the Chesapeake Bay nursery. The predicted consumption rates support the conclusion that juvenile sandbar sharks exert a lesser top-down effect on the Chesapeake Bay ecosystem than do teleost piscivores and hu

The Bait Purse-seine Fishery for Atlantic Menhaden, Brevoortia tyrannus, in the Virginia Portion of Chesapeake Bay

Through the mid 1990’s, the bait purse-seine fishery for Atlantic menhaden, Brevoortia tyrannus, in the Virginia portion of Chesapeake Bay was essentially undocumented. Beginning in 1995, captains of Virginia bait vessels maintained deck logs of their daily fishing activities; concurrently, we sampled the bait landings for size and age composition of the catch. Herein, we summarize 15 years (1995–2009) of data from the deck logbooks, including information on total bait landings by purse seine, proportion of fishing to nonfishing days, proportion of purse-seine sets assisted by spotter pilots, nominal fishing effort, median catches, and temporal and areal trends in catch. Age and size composition of the catch are described, as well as vessel and gear characteristics and disposition of the catch.

Reproductive seasonality, fecundity, and spawning frequency of tautog (Tautoga onitis) in the lower Chesapeake Bay and coastal waters of Virginia

The tautog, Tautoga onitis (Linnaeus), ranges from Nova Scotia to South Carolina and has become a popular target for recreational and commercial fisheries. Although tautog are a multiple spawning species, reproductive potential, measured as annual fecundity, has not been estimated previously with methods (batch fecundity, spawning frequency) necessary for a species with indeterminate annual fecundity. A total of 960 tautog were collected from the mouth of the Rappahannock River in the lower Chesapeake Bay to 45 km offshore of Virginia’s coastline to investigate tautog reproductive biology in the southern portion of the species range. Tautog did not exhibit a 1:1 sex ratio; 56% were females. Male tautog reached 50% maturity at 218 mm TL, females at 224 mm TL. Tautog spawned from 7 April 1995 to 15 June 1995, at locations from the York River to 45 km offshore. Batch fecundity estimates ranged from 2800 to 181,200 eggs per spawning for female tautog age 3–9, total length 259– 516 mm. Mean batch fecundity ±SEM for female tautog ages 4–6 was 54,243 ±2472 eggs and 106,256 ±3837 eggs for females ages 7–9. Spawning frequency was estimated at 1.2 days, resulting in 58 spawning days per female in 1995. Estimates of potential annual fecundity for tautog ages 3–9 ranged from 160,000 to 10,510,000 eggs.

Reconhecimento oficial de área livre do nematóide Anguina tritici (Steinbuch, 1799) Chitwood, 1935, no estado de Virginia; EUA, para exportação de trigo para o Brasil.

2002

The church's mission to the mountaineers of the South / by Archdeacon Neve, of Virginia, Archdeacon Spurr, of West Virginia, Archdeacon Wentworth, diocese of Lexington, Reverend S.C. Hughson, O.H.C., of Sewanee, Tenn., Reverend E.N. Joyner, diocese of Asheville, and Reverend W.S. Claiborne ; compiled by the Rev. Walter Hughson of the district of Asheville.

http://purl.oclc.org/KUK/KDL/B92-151-29579531

Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia.

BACKGROUND: West Virginia has the worst oral health in the United States, but the reasons for this are unclear. This pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. METHODS: Bacteria in subgingival plaque samples from twelve participants in two independent West Virginia dental-related studies were characterized using 16S rRNA gene sequencing and Human Oral Microbe Identification Microarray (HOMIM) analysis. Unifrac analysis was used to characterize phylogenetic differences between bacterial communities obtained from plaque of participants with low or high oral disease, which was further evaluated using clustering and Principal Coordinate Analysis. RESULTS: Statistically different bacterial signatures (P<0.001) were identified in subgingival plaque of individuals with low or high oral disease in West Virginia based on 16S rRNA gene sequencing. Low disease contained a high frequency of Veillonella and Streptococcus, with a moderate number of Capnocytophaga. High disease exhibited substantially increased bacterial diversity and included a large proportion of Clostridiales cluster bacteria (Selenomonas, Eubacterium, Dialister). Phylogenetic trees constructed using 16S rRNA gene sequencing revealed that Clostridiales were repeated colonizers in plaque associated with high oral disease, providing evidence that the oral environment is somehow influencing the bacterial signature linked to disease. CONCLUSIONS: Culture-independent analyses identified an atypical bacterial signature associated with high oral disease in West Virginians and provided evidence that the oral environment influenced this signature. Both findings provide insight into the etiology of the oral disparity in West Virginia.

Impacts of Mountaintop Removal Coal Mining on the Mud River, West Virginia: Selenium Accumulation, Trophic Transfer, and Toxicity in Fish

Selenium (Se) is a micronutrient necessary for the function of a variety of important enzymes; Se also exhibits a narrow range in concentrations between essentiality and toxicity. Oviparous vertebrates such as birds and fish are especially sensitive to Se toxicity, which causes reproductive impairment and defects in embryo development. Selenium occurs naturally in the Earth's crust, but it can be mobilized by a variety of anthropogenic activities, including agricultural practices, coal burning, and mining.

Mountaintop removal/valley fill (MTR/VF) coal mining is a form of surface mining found throughout central Appalachia in the United States that involves blasting off the tops of mountains to access underlying coal seams. Spoil rock from the mountain is placed into adjacent valleys, forming valley fills, which bury stream headwaters and negatively impact surface water quality. This research focused on the biological impacts of Se leached from MTR/VF coal mining operations located around the Mud River, West Virginia.

In order to assess the status of Se in a lotic (flowing) system such as the Mud River, surface water, insects, and fish samples including creek chub (Semotilus atromaculatus) and green sunfish (Lepomis cyanellus) were collected from a mining impacted site as well as from a reference site not impacted by mining. Analysis of samples from the mined site showed increased conductivity and Se in the surface waters compared to the reference site in addition to increased concentrations of Se in insects and fish. Histological analysis of mined site fish gills showed a lack of normal parasites, suggesting parasite populations may be disrupted due to poor water quality. X-ray absorption near edge spectroscopy techniques were used to determine the speciation of Se in insect and creek chub samples. Insects contained approximately 40-50% inorganic Se (selenate and selenite) and 50-60% organic Se (Se-methionine and Se-cystine) while fish tissues contained lower proportions of inorganic Se than insects, instead having higher proportions of organic Se in the forms of methyl-Se-cysteine, Se-cystine, and Se-methionine.

Otoliths, calcified inner ear structures, were also collected from Mud River creek chubs and green sunfish and analyzed for Se content using laser ablation inductively couple mass spectrometry (LA-ICP-MS). Significant differences were found between the two species of fish, based on the concentrations of otolith Se. Green sunfish otoliths from all sites contained background or low concentrations of otolith Se (< 1 µg/g) that were not significantly different between mined and unmined sites. In contrast creek chub otoliths from the historically mined site contained much higher (≥ 5 µg/g, up to approximately 68 µg/g) concentrations of Se than for the same species in the unmined site or for the green sunfish. Otolith Se concentrations were related to muscle Se concentrations for creek chubs (R2 = 0.54, p = 0.0002 for the last 20% of the otolith Se versus muscle Se) while no relationship was observed for green sunfish.

Additional experiments using biofilms grown in the Mud River showed increased Se in mined site biofilms compared to the reference site. When we fed fathead minnows (Pimephales promelas) on these biofilms in the laboratory they accumulated higher concentrations of Se in liver and ovary tissues compared to fathead minnows fed on reference site biofilms. No differences in Se accumulation were found in muscle from either treatment group. Biofilms were also centrifuged and separated into filamentous green algae and the remaining diatom fraction. The majority of Se was found in the diatom fraction with only about 1/3rd of total biofilm Se concentration present in the filamentous green algae fraction

Finally, zebrafish (Danio rerio) embryos were exposed to aqueous Se in the form of selenate, selenite, and L-selenomethionine in an attempt to determine if oxidative stress plays a role in selenium embryo toxicity. Selenate and selenite exposure did not induce embryo deformities (lordosis and craniofacial malformation). L-selenomethionine, however, induced significantly higher deformity rates at 100 µg/L compared to controls. Antioxidant rescue of L-selenomethionime induced deformities was attempted in embryos using N-acetylcysteine (NAC). Pretreatment with NAC significantly reduced deformities in the zebrafish embryos secondarily treated with L-selenomethionine, suggesting that oxidative stress may play a role in Se toxicity. Selenite exposure also induced a 6.6-fold increase in glutathione-S-transferase pi class 2 gene expression, which is involved in xenobiotic transformation. No changes in gene expression were observed for selenate or L-selenomethionine-exposed embryos.

The findings in this dissertation contribute to the understanding of how Se bioaccumulates in a lotic system and is transferred through a simulated foodweb in addition to further exploring oxidative stress as a potential mechanism for Se-induced embryo toxicity. Future studies should continue to pursue the role of oxidative stress and other mechanisms in Se toxicity and the biotransformation of Se in aquatic ecosystems.

Optimización en el uso de los recursos hídricos y nutricionales por el tabaco Virginia en el departamento de Chicoana (Salta)

El aprovechamiento de los nutrientes y el agua por el tabaco en lotes de pequeños productores de Chicoana (Salta) puede ser optimizado según lo sugieren diagnósticos preliminares. El objetivo de esta tesis fue investigar la dinámica de los nutrientes y el agua con el fin de optimizar el uso de dichos recursos. La dinámica de los nutrientes y el agua se investigó en condiciones de campo mientras que el ajuste de dichos factores para el tabaco se desarrolló en condiciones controladas. Los estudios de campo se realizaron en 13 lotes de pequeños productores los cuales fueron separados en dos grupos AP y BP (alta y baja producción). Antes y durante el cultivo se determinaron en suelo: textura, N-nitratos, N, P y K total y en planta: N, P y K absorbido y materia seca en planta entera, hoja, tallo y raíz del tabaco. En condiciones controladas se aplicó un diseño factorial (n=3) : MO inicial, fertilización según modelo zonal y nivel de riego. A cosecha, se evaluaron MS y N, P y K absorbidos. Los resultados mostraron que la MS, N, P y K absorbido en AP resultaron significativamente superiores a BP. La oferta de nutrientes fue muy superior a la demanda generándose excedentes en el balance de N. Las dosis de K y P aplicadas fueron superiores a las recomendadas aunque no se observaron excedentes en el suelo. El muy bajo nivel de P absorbido pudo deberse al escaso desarrollo radicular. El estudio de la dinámica del agua mostró que la disminución del 30 por ciento de rendimiento pudo relacionarse con el riego deficitario. El estudio en condiciones controladas demostró que los criterios utilizados (modelo generado en la zona y umbrales de humedad) permiten optimizar el uso de los nutrientes y el agua por el tabaco en lotes de pequeños productores de Chicoana.