Structural studies on tobacco streak virus coat protein: Insights into the pleomorphic nature of ilarviruses

Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33 nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from protecting the viral genome, CPs of ilarviruses play several key roles in the life cycle of these viruses. Unlike the related bromo and cucumoviruses, ilarvirus particles are labile and pleomorphic, which has posed difficulties in their crystallization and structure determination. In the current study, a truncated TSV-CP was crystallized in two distinct forms and their structures were determined at resolutions of 2.4 angstrom and 2.1 angstrom, respectively. The core of TSV CP was found to possess the canonical beta-barrel jelly roll tertiary structure observed in several other viruses. Dimers of CP with swapped C-terminal arms (C-arm) were observed in both the crystal forms. The C-arm was found to be flexible and is likely to be responsible for the polymorphic and pleomorphic nature of TSV capsids. Consistent with this observation, mutations in the hinge region of the C-arm that reduce the flexibility resulted in the formation of more uniform particles. TSV CP was found to be structurally similar to that of Alfalfa mosaic virus (AMV) accounting for similar mechanism of genome activation in alfamo and ilar viruses. This communication represents the first report on the structure of the CP from an ilarvirus. (C) 2015 Elsevier Inc. All rights reserved.

Strategies for the establishment of profitable fish farms in Nigeria

Cost projections for the establishment of a fish farm in Nigeria was conducted. It is shown that fish farming should be a lucrative venture. However, many private fish farms are not making the desired profits because of a variety of problems. Besides managerial incompetence, availability of fingerlings for stocking in the ponds is shown to be a serious handicap to the success of investments in a fish farm. It is suggested that where the funds are available, each farm should be equipped with facilities for raising its needs of fingerlings. Since useful capital would be involved and knowledgeable experts to run the hatcheries are few, it is recommended that fish farms within a state or adjacent states should combine to establish viable cooperative hatcheries

Development of a new modelling framework to estimate the C footprint from Basque dairy farms

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Applying systems ecology to the analysis of integrated agriculture-aquaculture farms

The article suggests a preliminary list of properties as a point of departure for quantifying various ecological facets of the integrated agriculture-aquaculture farms.

Effect of planting pattern on shading and phytoplankton photosynthesis in Bangladesh rice-fish farms

In the present study, a Border Planting (BP) pattern recommended by the Bangladesh Agricultural Research Council was tested against Regular Planting (RP) to assess the effect, if any, on gross phytoplankton photosynthesis and shading. A Wide Planting (WP) pattern was also included in the trial, as an extreme case, to assess the level of photosynthesis that might be obtained if some of the rice yield was sacrificed. Three rice-planting treatments, each with four replicates, using the rice variety BR16, were undertaken: (1) RP, 26 cm between rows N to S and E to W; (2) BP, 20 cm between rows N to S and alternately 21 cm and 36 cm between rows E, to W; and (3) WP, 66 cm between rows N to S and E to W. BP showed no advantage compared to RP, possibly because the rice variety used was short and thus produced relatively little shading. WP did show a benefit for phytoplankton photosynthesis, but this may not generate an increase in fish yield sufficient to justify the negative impact of rice production.

Rice-freshwater prawn (Macrobrachium rosenbergii) farms in the Mekong Delta, Vietnam

Aquaculture in riceland has been practiced in Mekong Delta, Vietnam for a long time and integrated rice-freshwater prawn (Macrobrachium rosenbergii) farming has become more and more popular. The integrated farming systems developed and practiced by farmers in the area to produce more food and more cash crops are presented and discussed.