Impact of preservation solution on the extent of blood-air barrier damage and edema formation in experimental lung transplantation

A major aim in lung transplantation is to prevent the loss of structural integrity due to ischemia and reperfusion (I/R) injury. Preservation solutions protect the lung against I/R injury to a variable extent. We compared the influence of two extracellular-type preservation solutions (Perfadex, or PX, and Celsior, or CE) on the morphological alterations induced by I/R. Pigs were randomly assigned to sham (n = 4), PX (n = 5), or CE (n = 2) group. After flush perfusion with PX or CE, donor lungs were excised and stored for 27 hr at 4 degrees C. The left donor lung was implanted into the recipient, reperfused for 6 hr, and, afterward, prepared for light and electron microscopy. Intra-alveolar, septal, and peribronchovascular edema as well as the integrity of the blood-air barrier were determined stereologically. Intra-alveolar edema was more pronounced in CE (219.80 +/- 207.55 ml) than in PX (31.46 +/- 15.75 ml). Peribronchovascular (sham: 13.20 +/- 4.99 ml; PX: 15.57 +/- 5.53 ml; CE: 31.56 +/- 5.78 ml) and septal edema (thickness of alveolar septal interstitium, sham: 98 +/- 33 nm; PX: 84 +/- 8 nm; CE: 249 +/- 85 nm) were only found in CE. The blood-air barrier was similarly well preserved in sham and PX but showed larger areas of swollen and fragmented epithelium or endothelium in CE. The present study shows that Perfadex effectively prevents intra-alveolar, septal, and peribronchovascular edema formation as well as injury of the blood-air barrier during I/R. Celsior was not effective in preserving the lung from morphological I/R injury.

Early Intermediate and Middle Horizon ceramic styles of the Cuzco Valley / Brian S. Bauer, Bradford M. Jones.

n.s. no.34(2003)

The grammar of ornament : illustrated by examples from various styles of ornament /

With contributions by J.B. Waring, J.O. Westwood and M.D. Wyatt.

An attempt to discriminate the styles of architecture in England, from the conquest to the reformation : with a sketch of the Grecian and Roman orders.

Mode of access: Internet.

Examples of ancient doorways and windows, arranged to illustrate the different styles of church architecture from the Conquest to the Reformation, from existing examples /

Sheet divided into 9 sections and mounted on cloth. Bound in red bookcloth, stamped in blind and gilt.

Old clocks and watches and their makers; being an historical and descriptive account of the different styles of clocks and watches of the past, in England and abroad, to which is added a list of eight thousand makers;

Mode of access: Internet.

The effect of solid solution additions on the thermal conductivity of UO₂ /

"Contract No. AT-30-1 Gen-366."

The life styles of nine American cocaine users : trips to the Land of Cockaigne /

Mode of access: Internet.

Styles of address, a manual of usage in writing and in speech ...

On cover: A Crowell reference book.

An attempt to discriminate the styles of architecture in England,

Mode of access: Internet.