A question of a voice: with what voice will I cry my sad fado?

This work is licensed under a Creative Commons Attribution 3.0 License.

Deformability Measurement of Single-Cells at High-Throughput With Imaging Flow Cytometry

Disease conditions like malaria, sickle cell anemia, diabetes mellitus, cancer, etc., are known to significantly alter the deformability of certain types of cells (red blood cells, white blood cells, circulating tumor cells, etc.). To determine the cellular deformability, techniques like micropipette aspiration, atomic force microscopy, optical tweezers, quantitative phase imaging have been developed. Many of these techniques have an advantage of determining the single cell deformability with ultrahigh precision. However, the suitability of these techniques for the realization of a deformability based diagnostic tool is questionable as they are expensive and extremely slow to operate on a huge population of cells. In this paper, we propose a technique for high-throughput (800 cells/s) determination of cellular deformability on a single cell basis. This technique involves capturing the image(s) of cells in flow that have undergone deformation under the influence of shear gradient generated by the fluid flowing through the microfluidic channels. Deformability indices of these cells can be computed by performing morphological operations on these images. We demonstrate the applicability of this technique for examining the deformability index on healthy, diabetic, and sphered red blood cells. We believe that this technique has a strong role to play in the realization of a potential tool that uses deformability as one of the important criteria in disease diagnosis.

The status of nine former C.I.D. students who are attending public high schools with hearing peers

This paper documents nine former CID students to evaluate the effectiveness of CID's program of preparation for mainstreaming. NOTE: Access to thesis is restricted. Contact Archives and Rare Books.

Early Planting of Tomatoes in a High Tunnel with Plant Coverings

High tunnels have been successfully used in Iowa to modify the climate and extend the growing season for tomatoes and other crops. Without the use of supplemental heat these ventilated, single layered plastic structures have typically increased average inside air temperatures by 10°F or more over outside temperatures for the growing season. The same tunnel, however, will only increase the daily low temperature by about 1 or 2°F, thus making early season high tunnel plantings without additional heat or plant coverings risky in Iowa. Fabric row covers are commonly used in high tunnels to provide for an additional 2-4°F frost protection during cold evenings. The recommended planting date for high tunnel tomatoes in Iowa has been about April 16 (4 to 5 weeks ahead of the recommended outside planting date). Producers are also advised to have some sort of plant covering material available to protect plants during a late spring frost.

Juncker raises high expectations with ambitious agenda. CEPS Commentary, 16 July 2014

Based on his speech to the European Parliament on July 15th, following his election as President of the European Commission, Jean-Claude Juncker has clearly opted for ‘more Union’ during his five-year term, offering up an ambitious agenda that raises a host of expectations. In this CEPS Commentary, Karel Lannoo outlines the most salient items on Juncker’s agenda, focusing on the most laudable and those that will pose the greatest challenges.

Gems of German song (revised edition) for low voice.

English and German words with piano accompaniment.

Tamul hymns set to German tunes : arranged for the voice with an accompaniment for the organ or piano = Krietucapaiyil valak̃iya ñāṉappāṭṭukaḷukkaṭutta almāṇiyarañkañkaḷ /

Mode of access: Internet.

Kindertotenlieder = Songs on the death of infants = Chants sur la mort d'enfants /

Duration: 20 min.