955 resultados para Soluble protein
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Este trabalho foi conduzido no Laboratório de Análise de Sementes, do Departamento de Produção Vegetal, da Universidade Estadual Paulista (Botucatu/SP), com o objetivo de avaliar os efeitos causados por diferentes períodos de envelhecimento acelerado na lixiviação de íons e de proteínas solúveis em sementes de milho. Sementes de milho do híbrido BR 3123 foram colocadas sobre tela em gerbox, contendo 40mL de água destilada, e mantidas a temperatura de 42°C por períodos de 0, 24, 48, 72, 96, 120, 144 e 166 horas. Após o envelhecimento, parte das sementes foi colocada para germinar, empregando quatro repetições para cada período, obtendo-se a porcentagem de plântulas normais no quarto dia. em outra parte realizou-se, após manutenção em copos plásticos com 75mL de H2O destilada por 24 horas à 25°C, leituras de condutividade elétrica (µS.cm-1.g-1), determinação de íons (mg.L-1.g de semente-1) e de proteínas solúveis totais (µproteina.g-1.mL-1) lixiviadas na solução. Os resultados indicaram que a elevação dos valores de condutividade elétrica e de lixiviado de proteínas totais ocorreu a partir de 72 horas de envelhecimento acelerado. A lixiviação de potássio foi mais acentuada que a dos íons cálcio, zinco, manganês, cobre, ferro e magnésio.
Resumo:
O objetivo do estudo foi determinar os conteúdos de saponinas, taninos e a solubilidade da proteína (SP) de 28 cultivares de alfafa: Crioula, Monarca, BR 4, Alto Great, MH 4, SW 9210 A, 5929, BR 1, EL Grande, 5715, MH 15, Valley Plus, BR 2, Rio, SW 8210, Maricopa, ICI 990, 5888, P 30, Alfa-200, WL 516, SW 8112 A, BR 3, Florida 77, Araucana, Falcon, Semit 921 e Sutter. O material analisado foi obtido do 10º e 14º corte, respectivamente; em 08/12/97 e 16/04/98, de um experimento desenvolvido na Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu. Amostras de cada cultivar foram colhidas, pesadas e secas em estufa a 52ºC. As saponinas foram extraídas com solvente hidrofílico e lipofílico e o conteúdo foi calculado pela equação y=0,8121x-1,4759, R² = 1,00. A extração dos taninos totais e condensados foi efetuada por meio de ultra-som (12 min), sendo os taninos totais determinados pela equação: y=44,978 + 0,5644 com R²=0,9977 e os condensados, multiplicando-se a absorbância por 78,26 dividido pelo teor de matéria seca. A SP foi determinada pelo método de KOH, de acordo com a seguinte fórmula: SP (%) = proteína solúvel x 100/proteína bruta da amostra. Os teores de saponinas, taninos totais e condensados e a solubilidade da proteína não diferiram (P>0,05) entre as cultivares. Houve efeito (P<0,05) da época de corte apenas sobre o teor de taninos totais. Os teores médios de saponinas de 1,00% aliados à baixa solubilidade média da proteína bruta (34,11%) não se constituem em fatores limitantes para uso dos cultivares de alfafa estudadas.
Resumo:
Tabebuia roseo-alba is a native Brazilian tree species with wide occurrence, particularly in semi-deciduous forest. The tree has lush flowering and is used in landscaping, arboriculture and for reforestation of dry and rocky soils. Its seeds are small, lightweight and winged, and are wind-dispersed. The objective of this study was to investigate the biochemical changes in T. roseo-alba seeds during germination. We determined the content of starch, structural polysaccharides, lipid and soluble protein, and analysed the proteins by SDS-PAGE. There was a rapid consumption of starch, structural polysaccharides, lipids and proteins during germination of T. roseo-alba seeds. The rapid mobilisation of protein during germination, was accompanied by the synthesis of high molecular mass proteins and degradation of low molecular mass proteins.
Resumo:
Although mineral nutrition affects maize (Zea mays L.) yield by controlling starch deposition in kernels, the mechanisms involved are largely unknown. Our objectives were to examine this relationship by nutritionally and genetically altering starch production in the endosperm. Kernels of W64A and two starch-deficient mutants, shrunken-1 and brittle-2, were grown in vitro with varying supplies of N (0-50 mM) or P (0-6 mM) to produce different degrees of endosperm starch production, and the levels of enzyme activities and metabolites associated with carbohydrate and N metabolism were examined. In vitro grown kernels exhibited the expected starch phenotypes, and a minimum level of media N (25 mM) and P (2 mM) was required for optimal growth. However, increasing the availability of N or P could not overcome the genetically induced decrease in starch deposition of the mutants. Nitrogen deficiency enhanced sugar accumulation, but decreased amino acid levels, soluble protein, enzyme activity, starch synthesis, and endosperm dry weight. Phosphorous deficiency also decreased starch production and endosperm dry weight, but with only a minimal effect on the activities of ADP-glucose pyrophosphorylase and alanine transaminase. Genotypic differences in endosperm starch, and the increases induced by N and P supply, Here closely associated with the level of endosperm N, but not endosperm P. Thus, while both N and P are crucial for optimal yield of maize grain, they appear to act by different means, and with different importance in governing starch deposition in the endosperm.
Resumo:
The aim of this work was to evaluate the effects of aluminum on the growth of Eucalyptus shoots cultivated in vitro through nutrient and total soluble protein content. The trial had a totally randomized design with four treatments and four replicates. The treatments were: 0.0; 0.25; 0.5 and 1.0 mM of AlCl 3.6H 2O. Shoots without roots of a Eucalyptus grandis x E urophylla clone were used for the in vitro culture. Evaluations were made on the 4th, 8th, 12th, 16th, 20th, 24th and 28th day of culture. The Al addition to the culture media reduced mainly Ca, P and K availability and absorptions by the shoots. The cellular metabolism was affected, conducted to morphological alterations in shoots (browning, mass calluses formation and shoots not friable), dry matter increased and a decreased in total protein soluble.
Resumo:
Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 → 6)β-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/ 11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. Copyright by the Brazilian Society of Genetics.
Resumo:
The present work was carried out at the Faculdade de Ciências Agronômicas - UNESP, Botucatu, SP. The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. Sweet pepper plants were grown for 230 days after transplanting of seedlings. The experiment was arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated, as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving the plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.
Resumo:
The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11. 1.7) activity as an indicator of water stress in plants. The experiment was carried out at the Faculdade de Ciências Agronômicas UNESP, Botucatu, SP. Sweet pepper plants were grown for 230 days after transplanting of seedlings and arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants.
Resumo:
Nitrogen limitation in the common bean (Phaseolus vulgaris L.) produces reduced productivity and seeds with low protein content and physiological quality. We assessed the effects of nitrogen fertilizer side dressing on the protein content and physiological quality of the seeds of P. vulgaris L. cultivars (cv.) IPR Juriti and Pérola grown in plots (soil type = Dystrophic Red Latosol) using a no-tillage system under a thick mulch of millet residues at the Experimental Station of UNESP-Ilha Solteira campus, located in Selvíria, MS (Köppen climate type = Aw) during autumn (March/June) and winter (June/September) 2005. For each cultivar, a randomized block experimental design was used with four replicates and factorially arranged treatments equivalent to 0, 30, 60, 90 and 120 kg.ha-1 added nitrogen (as urea, containing 45% of N) applied as a side dressing during the V4-3 and V4-6 phenological stage corresponding to the 3rd and 6th completely opened trifoliolate leaf on the main stem. Supplementation with up to 120 kg.ha-1 nitrogen promoted a greater increase in crude protein at V4-3 (unsupplemented = 17.6% and 16.3%; 120 kg.ha-1 N = 24.1% and 22.3% for cv. IPR Juriti and Pérola, respectively) than at V4-6 (unsupplemented = 19.2% and 18.3%; 120 kg.ha-1 N = 21.3% and 20.3% for cv. IPR Juriti and Pérola, respectively). About 90% of the crude protein from cv. IPR Juriti was composed of soluble protein compared to 72% for the cv. Pérola. Albumins and globulins represented about 80% of the soluble protein and prolamins were lower at 0.6%. In conclusion, nitrogen fertilization up to 120 kg.ha-1 applied as a side dressing at the V4-3 phenological stage in no-tillage under a thick mulch of millet promoted a greater increase of crude protein in common bean seeds than at the V4-6 stage. The highest accumulation of soluble protein occurred at 90 kg.ha-1 applied nitrogen without having a significant influence on the physiological quality of the seeds.
Resumo:
The aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures.
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
